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The map label for this gene is aes [H]

Identifier: 238920842

GI number: 238920842

Start: 2856051

End: 2857022

Strand: Reverse

Name: aes [H]

Synonym: NT01EI_2968

Alternate gene names: 238920842

Gene position: 2857022-2856051 (Counterclockwise)

Preceding gene: 238920844

Following gene: 238920835

Centisome position: 74.94

GC content: 59.67

Gene sequence:

>972_bases
ATGCAACAGAAGAATAAAGTAAATGTTCCTGAGCGTATTTCTGATGAAATGAAGCGTGTTATGCAATTTCAGCAGCAGAA
TACAACGGATGAACAGATTTGCTTAGATTATTCGTCGATGCGTCAGGCCTATATTCAGGAACGGCAGTATTGGAATCAGG
GCGGCGTCGAAATGGCCCGCTGTGAGCAGGTCAGCGTGGAGACGCCCCGCGGCCCGGTTGCGACGCGTATCTATTTTCCG
CGTCATCCGGCGCAGGCGGTGCTGTTCTATCTGCACGGCGGCGGATTTATTGTGGGTAATCTGGATACCCACGATCGCAT
TATGCGTCTGCTGGCCGACTACAGCCGCTGCGCGGTGATCGGTATAGATTACAGCCTGTCGCCGGAGGCGCGCTTCCCGC
AGGCTATCGAGGAGAGCGTCGCCGTCTGTGACTTCTTTCATCGCCGCGCCGGTGAGTACGGGCTGCCGATGGCGCGGATT
GGCTTTGCCGGCGACTCCGCCGGGGCCATGCTGGCGATGGCGACGGTGCTGTGGATGCGCGATCGCGGGATCGACTGCGG
CGAGGTGCGCGGGGTGCTGCTCTACTACGGTCTGTATGGTCTGCGGGATTCGGCCAGCCGTCGTCTGTACGGCGGCGTTT
GGGATGGGCTCACCCAGGCGGATCTGTTGAGCTATGAGCAGGCCTATCTGCCGGATGCGGCGTCGCGGGAGTCGCCCTAT
TACTGTCTGTTTAACAACGATCTCACCCATGACATTCCCCCCTGCTTTATCGCCGGGGCGCAGTTCGATCCGCTGTTGGA
CGACAGCGTGGCGCTGTACCAGACGCTGCGCGAACACCGCCAGCCCTGCAGCTACCACATGTATCCCGGCGTGATGCACG
CCTTTCTGCACTACTCCCGCATGATGGAGAGCGCCGACCGGGCCCTGCGCGACGGCGCTGACTATTTTTACCGTCAGCTT
CAGATTGCCTGA

Upstream 100 bases:

>100_bases
TAATCCTATTATTTATAATGGTTTTTTCTGCTTATGGCGTAGCTCACAGAACCCATTTCTGTATATCATAAAATATCAGT
TAATCGGTTGGGAGAACGGT

Downstream 100 bases:

>100_bases
TGCGCAGCGGGTAGCCCTGGGGCTACCCGCCGATTCGTGGCCGACCTGCCCGCCCACAGGGCGGGACGGCGTTAAAACAG
TTTCATCCGGCTGGTTTGCT

Product: acetyl esterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MQQKNKVNVPERISDEMKRVMQFQQQNTTDEQICLDYSSMRQAYIQERQYWNQGGVEMARCEQVSVETPRGPVATRIYFP
RHPAQAVLFYLHGGGFIVGNLDTHDRIMRLLADYSRCAVIGIDYSLSPEARFPQAIEESVAVCDFFHRRAGEYGLPMARI
GFAGDSAGAMLAMATVLWMRDRGIDCGEVRGVLLYYGLYGLRDSASRRLYGGVWDGLTQADLLSYEQAYLPDAASRESPY
YCLFNNDLTHDIPPCFIAGAQFDPLLDDSVALYQTLREHRQPCSYHMYPGVMHAFLHYSRMMESADRALRDGADYFYRQL
QIA

Sequences:

>Translated_323_residues
MQQKNKVNVPERISDEMKRVMQFQQQNTTDEQICLDYSSMRQAYIQERQYWNQGGVEMARCEQVSVETPRGPVATRIYFP
RHPAQAVLFYLHGGGFIVGNLDTHDRIMRLLADYSRCAVIGIDYSLSPEARFPQAIEESVAVCDFFHRRAGEYGLPMARI
GFAGDSAGAMLAMATVLWMRDRGIDCGEVRGVLLYYGLYGLRDSASRRLYGGVWDGLTQADLLSYEQAYLPDAASRESPY
YCLFNNDLTHDIPPCFIAGAQFDPLLDDSVALYQTLREHRQPCSYHMYPGVMHAFLHYSRMMESADRALRDGADYFYRQL
QIA
>Mature_323_residues
MQQKNKVNVPERISDEMKRVMQFQQQNTTDEQICLDYSSMRQAYIQERQYWNQGGVEMARCEQVSVETPRGPVATRIYFP
RHPAQAVLFYLHGGGFIVGNLDTHDRIMRLLADYSRCAVIGIDYSLSPEARFPQAIEESVAVCDFFHRRAGEYGLPMARI
GFAGDSAGAMLAMATVLWMRDRGIDCGEVRGVLLYYGLYGLRDSASRRLYGGVWDGLTQADLLSYEQAYLPDAASRESPY
YCLFNNDLTHDIPPCFIAGAQFDPLLDDSVALYQTLREHRQPCSYHMYPGVMHAFLHYSRMMESADRALRDGADYFYRQL
QIA

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=123, Percent_Identity=36.5853658536585, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI206597554, Length=134, Percent_Identity=33.5820895522388, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786682, Length=320, Percent_Identity=63.75, Blast_Score=420, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71996133, Length=245, Percent_Identity=25.7142857142857, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17540028, Length=198, Percent_Identity=29.2929292929293, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI72001146, Length=127, Percent_Identity=31.496062992126, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24656084, Length=117, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24656076, Length=117, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI20130169, Length=117, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.-

Molecular weight: Translated: 36909; Mature: 36909

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS01173 LIPASE_GDXG_HIS ; PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQKNKVNVPERISDEMKRVMQFQQQNTTDEQICLDYSSMRQAYIQERQYWNQGGVEMAR
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
CEQVSVETPRGPVATRIYFPRHPAQAVLFYLHGGGFIVGNLDTHDRIMRLLADYSRCAVI
HCCCCCCCCCCCCEEEEECCCCCHHEEEEEEECCEEEEECCCHHHHHHHHHHHCCCEEEE
GIDYSLSPEARFPQAIEESVAVCDFFHRRAGEYGLPMARIGFAGDSAGAMLAMATVLWMR
EECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHEECCCCCCHHHHHHHHHHHHH
DRGIDCGEVRGVLLYYGLYGLRDSASRRLYGGVWDGLTQADLLSYEQAYLPDAASRESPY
HCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCHHHCCCHHHHHHHHHHHHCCCCCCCCCCE
YCLFNNDLTHDIPPCFIAGAQFDPLLDDSVALYQTLREHRQPCSYHMYPGVMHAFLHYSR
EEEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
MMESADRALRDGADYFYRQLQIA
HHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MQQKNKVNVPERISDEMKRVMQFQQQNTTDEQICLDYSSMRQAYIQERQYWNQGGVEMAR
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
CEQVSVETPRGPVATRIYFPRHPAQAVLFYLHGGGFIVGNLDTHDRIMRLLADYSRCAVI
HCCCCCCCCCCCCEEEEECCCCCHHEEEEEEECCEEEEECCCHHHHHHHHHHHCCCEEEE
GIDYSLSPEARFPQAIEESVAVCDFFHRRAGEYGLPMARIGFAGDSAGAMLAMATVLWMR
EECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHEECCCCCCHHHHHHHHHHHHH
DRGIDCGEVRGVLLYYGLYGLRDSASRRLYGGVWDGLTQADLLSYEQAYLPDAASRESPY
HCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCHHHCCCHHHHHHHHHHHHCCCCCCCCCCE
YCLFNNDLTHDIPPCFIAGAQFDPLLDDSVALYQTLREHRQPCSYHMYPGVMHAFLHYSR
EEEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
MMESADRALRDGADYFYRQLQIA
HHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA