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The map label for this gene is caiA [H]

Identifier: 238920833

GI number: 238920833

Start: 2847827

End: 2848969

Strand: Reverse

Name: caiA [H]

Synonym: NT01EI_2959

Alternate gene names: 238920833

Gene position: 2848969-2847827 (Counterclockwise)

Preceding gene: 238920834

Following gene: 238920832

Centisome position: 74.73

GC content: 61.42

Gene sequence:

>1143_bases
ATGGATTTTACACTGACTGACGAGCAGGAGCTGTTCGTTGCCGGGATTCGCGATCTGATGGCGCAGGAGAACTGGGAAGC
CTACTTTGCCGAGTGCGATCGCAACAGCGAATACCCTGAGCGTTTTGTTAAGGCACTGGCGGATATGGGCATCGACTCAC
TGCTGCTGCCGGAGGAGCAGGGCGGACTGGACGCCGGGTTTGTCACCCTGGCCGCGGTCTGGATGGAGCTGGGCCGTCTG
GGGGCGCCGACCTATGTGCTGTACCAGCTGCCGGGCGGGTTTAACACCGTCTTGCGCGAGGGCACCGCAGAGCAGATCGA
CCGCATCATGGCCTTCCGCGGTACCGGCAAGCAAATGTGGAACTCTGCGATCACCGAGCCGGGCGCTGGCTCCGACGTGG
GCAGCCTGCAGACCACCTATACGCGCAAGAACGGCAAGGTCTACCTCAACGGCAGCAAGTGCTTTATCACCAGCAGCGCC
TATACCCCGTATCTGGTGGTGATGGCGCGCGACGGCGCCTCGCCGGAGAAAGCGGTCTACTCCGAGTGGTTTGTCGATAT
GAGCCTGCCGGGCATCAAGGTCAACAAGCTGGAGAAGCTGGGCCTGCGCATGGACAGCTGCTGTGAGGTGGTATTCGACA
ACGTCGAGCTGGAGGAGAAGGATCTGTTCGGGCGCGAAGGCAACGGCTTCAACCGGGTGAAAGAGGAGTTCGATCATGAA
CGCTTCCTGGTGGCGCTGACCAACTATGGCACCGCGCTGTGCGCCTTTGAGGACGCCGCGCGCTACGCCAATCAGCGCGT
GCAGTTCGGCGAAACCATCGGCCGCTTCCAGTTGATCCAGGAGAAGTTCGCCCACATGGCGATCAAGCTGAACTCCATGA
AGAACATGCTGCTGGAGTCTGCCTGGAAGAGCGACAACGGCTTGATGACCTCCGGCGATGCGGCGATGTGTAAATACTTC
TGCGCCAACGCCGCCTTCGAGGTCGTGGATACCGCGATGCAAGTCCTGGGTGGAGTAGGGATCGCCGGCGATCACCGCAT
CACCCGCTTCTGGCGCGATCTGCGCGTGGACCGCGTTTCCGGCGGCTCCGACGAGATGCAGATCCTGACCCTGGGCCGCG
CGGTGCTGAAGCAGTATCGCTAA

Upstream 100 bases:

>100_bases
TCTTTGTCAACATATTGATAACCATATCGTTCTTGAAAGATGCCAAGTCCACGTACTGGAAATATTAACCCGTTAATTTG
CCAAACAAGAGGTCTGACAC

Downstream 100 bases:

>100_bases
GGGCCGCAGACGGCCGCCGCGGCGACCGTCTGTCTGCCATGACACCCGGGATCAGCCTGTATGCCCGGCCCCGGGTCCGC
TGATTTTTTTAAGAAGGATC

Product: crotonobetainyl-CoA dehydrogenase

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA reductase [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MDFTLTDEQELFVAGIRDLMAQENWEAYFAECDRNSEYPERFVKALADMGIDSLLLPEEQGGLDAGFVTLAAVWMELGRL
GAPTYVLYQLPGGFNTVLREGTAEQIDRIMAFRGTGKQMWNSAITEPGAGSDVGSLQTTYTRKNGKVYLNGSKCFITSSA
YTPYLVVMARDGASPEKAVYSEWFVDMSLPGIKVNKLEKLGLRMDSCCEVVFDNVELEEKDLFGREGNGFNRVKEEFDHE
RFLVALTNYGTALCAFEDAARYANQRVQFGETIGRFQLIQEKFAHMAIKLNSMKNMLLESAWKSDNGLMTSGDAAMCKYF
CANAAFEVVDTAMQVLGGVGIAGDHRITRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR

Sequences:

>Translated_380_residues
MDFTLTDEQELFVAGIRDLMAQENWEAYFAECDRNSEYPERFVKALADMGIDSLLLPEEQGGLDAGFVTLAAVWMELGRL
GAPTYVLYQLPGGFNTVLREGTAEQIDRIMAFRGTGKQMWNSAITEPGAGSDVGSLQTTYTRKNGKVYLNGSKCFITSSA
YTPYLVVMARDGASPEKAVYSEWFVDMSLPGIKVNKLEKLGLRMDSCCEVVFDNVELEEKDLFGREGNGFNRVKEEFDHE
RFLVALTNYGTALCAFEDAARYANQRVQFGETIGRFQLIQEKFAHMAIKLNSMKNMLLESAWKSDNGLMTSGDAAMCKYF
CANAAFEVVDTAMQVLGGVGIAGDHRITRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR
>Mature_380_residues
MDFTLTDEQELFVAGIRDLMAQENWEAYFAECDRNSEYPERFVKALADMGIDSLLLPEEQGGLDAGFVTLAAVWMELGRL
GAPTYVLYQLPGGFNTVLREGTAEQIDRIMAFRGTGKQMWNSAITEPGAGSDVGSLQTTYTRKNGKVYLNGSKCFITSSA
YTPYLVVMARDGASPEKAVYSEWFVDMSLPGIKVNKLEKLGLRMDSCCEVVFDNVELEEKDLFGREGNGFNRVKEEFDHE
RFLVALTNYGTALCAFEDAARYANQRVQFGETIGRFQLIQEKFAHMAIKLNSMKNMLLESAWKSDNGLMTSGDAAMCKYF
CANAAFEVVDTAMQVLGGVGIAGDHRITRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma- butyrobetainyl-CoA [H]

COG id: COG1960

COG function: function code I; Acyl-CoA dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557233, Length=379, Percent_Identity=28.7598944591029, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI4501859, Length=378, Percent_Identity=26.4550264550265, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI226958412, Length=380, Percent_Identity=28.4210526315789, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI4557231, Length=388, Percent_Identity=27.319587628866, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI187960098, Length=388, Percent_Identity=27.319587628866, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI226958414, Length=345, Percent_Identity=28.1159420289855, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI21361497, Length=363, Percent_Identity=30.8539944903581, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI4501857, Length=380, Percent_Identity=25.5263157894737, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI76496475, Length=337, Percent_Identity=29.080118694362, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4557235, Length=337, Percent_Identity=29.080118694362, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI7656849, Length=381, Percent_Identity=25.7217847769029, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI4503943, Length=373, Percent_Identity=24.3967828418231, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI7669494, Length=352, Percent_Identity=24.1477272727273, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI38505218, Length=344, Percent_Identity=24.1279069767442, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI48976061, Length=296, Percent_Identity=24.3243243243243, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI211971008, Length=296, Percent_Identity=24.3243243243243, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1786223, Length=380, Percent_Identity=90.7894736842105, Blast_Score=733, Evalue=0.0,
Organism=Escherichia coli, GI87081958, Length=383, Percent_Identity=48.8250652741514, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI87082384, Length=268, Percent_Identity=28.3582089552239, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17538396, Length=382, Percent_Identity=31.151832460733, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17569725, Length=368, Percent_Identity=26.9021739130435, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17534899, Length=393, Percent_Identity=26.2086513994911, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17508101, Length=380, Percent_Identity=25.7894736842105, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17570075, Length=384, Percent_Identity=26.8229166666667, Blast_Score=137, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17506239, Length=381, Percent_Identity=26.509186351706, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71985184, Length=380, Percent_Identity=27.1052631578947, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17533517, Length=362, Percent_Identity=25.6906077348066, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17551932, Length=370, Percent_Identity=25.4054054054054, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17505929, Length=362, Percent_Identity=24.8618784530387, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71990804, Length=328, Percent_Identity=25.609756097561, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71985192, Length=321, Percent_Identity=26.1682242990654, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17534353, Length=378, Percent_Identity=25.9259259259259, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI32563615, Length=231, Percent_Identity=30.7359307359307, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71982178, Length=276, Percent_Identity=21.7391304347826, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17562446, Length=334, Percent_Identity=22.7544910179641, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17531909, Length=248, Percent_Identity=20.9677419354839, Blast_Score=65, Evalue=8e-11,
Organism=Drosophila melanogaster, GI21356377, Length=379, Percent_Identity=29.287598944591, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24646207, Length=348, Percent_Identity=29.3103448275862, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI21355753, Length=344, Percent_Identity=30.2325581395349, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24666513, Length=380, Percent_Identity=27.6315789473684, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI281363737, Length=376, Percent_Identity=29.5212765957447, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24660351, Length=382, Percent_Identity=27.4869109947644, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI19920834, Length=280, Percent_Identity=27.1428571428571, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 42465; Mature: 42465

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00072 ACYL_COA_DH_1 ; PS00073 ACYL_COA_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFTLTDEQELFVAGIRDLMAQENWEAYFAECDRNSEYPERFVKALADMGIDSLLLPEEQ
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCEECCCCC
GGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTVLREGTAEQIDRIMAFRGTGKQMW
CCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHCCCCHHHH
NSAITEPGAGSDVGSLQTTYTRKNGKVYLNGSKCFITSSAYTPYLVVMARDGASPEKAVY
HHHCCCCCCCCCCCCCEEEEEECCCEEEECCCEEEEEECCCCCEEEEEECCCCCHHHHHH
SEWFVDMSLPGIKVNKLEKLGLRMDSCCEVVFDNVELEEKDLFGREGNGFNRVKEEFDHE
HHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHCCCC
RFLVALTNYGTALCAFEDAARYANQRVQFGETIGRFQLIQEKFAHMAIKLNSMKNMLLES
EEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWKSDNGLMTSGDAAMCKYFCANAAFEVVDTAMQVLGGVGIAGDHRITRFWRDLRVDRVS
HHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
GGSDEMQILTLGRAVLKQYR
CCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDFTLTDEQELFVAGIRDLMAQENWEAYFAECDRNSEYPERFVKALADMGIDSLLLPEEQ
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCEECCCCC
GGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTVLREGTAEQIDRIMAFRGTGKQMW
CCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHCCCCHHHH
NSAITEPGAGSDVGSLQTTYTRKNGKVYLNGSKCFITSSAYTPYLVVMARDGASPEKAVY
HHHCCCCCCCCCCCCCEEEEEECCCEEEECCCEEEEEECCCCCEEEEEECCCCCHHHHHH
SEWFVDMSLPGIKVNKLEKLGLRMDSCCEVVFDNVELEEKDLFGREGNGFNRVKEEFDHE
HHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHCCCC
RFLVALTNYGTALCAFEDAARYANQRVQFGETIGRFQLIQEKFAHMAIKLNSMKNMLLES
EEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWKSDNGLMTSGDAAMCKYFCANAAFEVVDTAMQVLGGVGIAGDHRITRFWRDLRVDRVS
HHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
GGSDEMQILTLGRAVLKQYR
CCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA