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The map label for this gene is asnB [H]

Identifier: 238920794

GI number: 238920794

Start: 2807029

End: 2808690

Strand: Direct

Name: asnB [H]

Synonym: NT01EI_2913

Alternate gene names: 238920794

Gene position: 2807029-2808690 (Clockwise)

Preceding gene: 238920793

Following gene: 238920795

Centisome position: 73.63

GC content: 61.67

Gene sequence:

>1662_bases
ATGTGTTCGATCTTTGGAGTGCTCGACCTGAAAACCGACCCACAGGCGCTGCGCAAAACAGCGCTGGAGATGTCCCGCCT
GATGCGCCACCGCGGCCCGGACTGGTCCGGCATCTATGCCGGTGAAAACGCCATTCTGGCCCACGAGCGCCTGTCTATCG
TCGACGTCAACACCGGCGCCCAGCCGCTGTATAACGCACAGCGCACCCATGTTCTCGCGGTCAACGGCGAAATTTACAAC
CACCAGGCGCTGCGCGAGCGCCTCGGCGATCGCTATGCGTTTCAGACCGGCTCCGACTGTGAGGTGATCCTGGCGCTGTA
CCAGGAGTATGGTCCGGACTTCCTCGACCAGCTACGCGGCATGTTTGCCTTTATTCTGTATGACGCCGAACGGGATGCCT
ATCTGATCGGCCGCGATCACCTGGGGATCATCCCGCTGTACATGGGACACGATGAACACGGCAATCTATTTGTCGCCTCA
GAGATGAAGGCGCTGGTGCCGGTATGTCGGACGCTGCGCGAATTCCCGGCCGGCAGTGCCCTGTGGAGCCGCGACGGCGA
AATCCGCCGCTACTACAGGCGCGACTGGTTTGACTACGCCGCCGTCGAACATAACCCTACCGACGCTGACGTGCTGCGCT
GTGCGCTGGAAGAGTCAGTCAAAAGCCATCTGATGTCTGACGTGCCCTACGGCGTACTGCTCTCCGGCGGGCTGGACTCT
TCGATTATCTCTGCCATCACCAAGAAGTTCGCCGCTCGCCGCGTCGAGGACGGCGAGCGCAGCGCCGCCTGGTGGCCACA
GCTGCACTCCTTTGCCATCGGCCTACAGGGCGCGCCGGATCTGAAGGCGGCTCAAGAGGTCGCCAACCATCTCGGCACGG
TACACCATGAGATCCACTTCACCGAGCAGGAGGGAATGGACGCCATCCGCGACGTGATTTATCACATCGAAACCTACGAC
GTCACCACCATCCGCGCCTCGACACCGATGTACCTAATGGCGCGCAAGATCAAGGCGATGGGGATCAAGATGGTGCTCTC
CGGCGAAGGTGCCGATGAGGTGTTCGGCGGCTACCTCTATTTCCATAAGGCGCCCAACGCCAAAGAGTTTCATGACGAGA
CGGTGCGCAAACTGCTGGCGCTGCACATGTATGACTGCGCGCGCGCCAACAAGGCGATGGCCGCCTGGGGCGTCGAAGCC
CGGGTACCGTTCCTCGATAAGACATTTCTCGACGTGGCCATGCGCCTCAACCCGCGCGACAAGATGTGCGGCAACGGACG
CATCGAAAAGCAGCTGCTGCGCGAATGTTTCGAATCCTATCTGCCGGCCAGCATCGCCTGGCGTCAGAAAGAGCAGTTCT
CCGACGGCGTTGGCTACAGCTGGATAGACTCGCTGAAAGCCGCCGCCGCCGCCCAGGTCAGCGACCAGACGCTGGCCAAT
GCCCGCTTCCGTTTTCCCTACAACACGCCAACCAGCAAGGAGGCCTACCTGTACCGCGATATCTTTGAGCAGCTGTTCCC
GCTGCCGAGTGCCGCAGAGTGCGTCCCCGGCGGCCCCTCCGTCGCGTGCTCCTCCGCCAAGGCCATCGAGTGGGACGAAT
CCCTGCGTAAAATGGACGACCCTTCCGGCCGCGCCGTCGGCGTACACAAGTCCGCCTATTAA

Upstream 100 bases:

>100_bases
ATTTACGTTCAGCTGACGGTGCATGTTGATATCATACGCAGCGCCGCAAGGGCGCCGTACCGTCGCAATCTGATACCGTC
AATGCCGTAGGGGAAACGCT

Downstream 100 bases:

>100_bases
CTCCTGCGGCGTGGCGTCTCCCCTGCGGGTTCGCCGCGCCGACTCCCTCTGCCATGCCGGTGTTAGCCTACTTTTCACCG
AGTTTTGCTGCTTTTCACGC

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MCSIFGVLDLKTDPQALRKTALEMSRLMRHRGPDWSGIYAGENAILAHERLSIVDVNTGAQPLYNAQRTHVLAVNGEIYN
HQALRERLGDRYAFQTGSDCEVILALYQEYGPDFLDQLRGMFAFILYDAERDAYLIGRDHLGIIPLYMGHDEHGNLFVAS
EMKALVPVCRTLREFPAGSALWSRDGEIRRYYRRDWFDYAAVEHNPTDADVLRCALEESVKSHLMSDVPYGVLLSGGLDS
SIISAITKKFAARRVEDGERSAAWWPQLHSFAIGLQGAPDLKAAQEVANHLGTVHHEIHFTEQEGMDAIRDVIYHIETYD
VTTIRASTPMYLMARKIKAMGIKMVLSGEGADEVFGGYLYFHKAPNAKEFHDETVRKLLALHMYDCARANKAMAAWGVEA
RVPFLDKTFLDVAMRLNPRDKMCGNGRIEKQLLRECFESYLPASIAWRQKEQFSDGVGYSWIDSLKAAAAAQVSDQTLAN
ARFRFPYNTPTSKEAYLYRDIFEQLFPLPSAAECVPGGPSVACSSAKAIEWDESLRKMDDPSGRAVGVHKSAY

Sequences:

>Translated_553_residues
MCSIFGVLDLKTDPQALRKTALEMSRLMRHRGPDWSGIYAGENAILAHERLSIVDVNTGAQPLYNAQRTHVLAVNGEIYN
HQALRERLGDRYAFQTGSDCEVILALYQEYGPDFLDQLRGMFAFILYDAERDAYLIGRDHLGIIPLYMGHDEHGNLFVAS
EMKALVPVCRTLREFPAGSALWSRDGEIRRYYRRDWFDYAAVEHNPTDADVLRCALEESVKSHLMSDVPYGVLLSGGLDS
SIISAITKKFAARRVEDGERSAAWWPQLHSFAIGLQGAPDLKAAQEVANHLGTVHHEIHFTEQEGMDAIRDVIYHIETYD
VTTIRASTPMYLMARKIKAMGIKMVLSGEGADEVFGGYLYFHKAPNAKEFHDETVRKLLALHMYDCARANKAMAAWGVEA
RVPFLDKTFLDVAMRLNPRDKMCGNGRIEKQLLRECFESYLPASIAWRQKEQFSDGVGYSWIDSLKAAAAAQVSDQTLAN
ARFRFPYNTPTSKEAYLYRDIFEQLFPLPSAAECVPGGPSVACSSAKAIEWDESLRKMDDPSGRAVGVHKSAY
>Mature_553_residues
MCSIFGVLDLKTDPQALRKTALEMSRLMRHRGPDWSGIYAGENAILAHERLSIVDVNTGAQPLYNAQRTHVLAVNGEIYN
HQALRERLGDRYAFQTGSDCEVILALYQEYGPDFLDQLRGMFAFILYDAERDAYLIGRDHLGIIPLYMGHDEHGNLFVAS
EMKALVPVCRTLREFPAGSALWSRDGEIRRYYRRDWFDYAAVEHNPTDADVLRCALEESVKSHLMSDVPYGVLLSGGLDS
SIISAITKKFAARRVEDGERSAAWWPQLHSFAIGLQGAPDLKAAQEVANHLGTVHHEIHFTEQEGMDAIRDVIYHIETYD
VTTIRASTPMYLMARKIKAMGIKMVLSGEGADEVFGGYLYFHKAPNAKEFHDETVRKLLALHMYDCARANKAMAAWGVEA
RVPFLDKTFLDVAMRLNPRDKMCGNGRIEKQLLRECFESYLPASIAWRQKEQFSDGVGYSWIDSLKAAAAAQVSDQTLAN
ARFRFPYNTPTSKEAYLYRDIFEQLFPLPSAAECVPGGPSVACSSAKAIEWDESLRKMDDPSGRAVGVHKSAY

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI296010848, Length=520, Percent_Identity=37.6923076923077, Blast_Score=303, Evalue=3e-82,
Organism=Homo sapiens, GI168229248, Length=520, Percent_Identity=37.6923076923077, Blast_Score=303, Evalue=4e-82,
Organism=Homo sapiens, GI168229252, Length=520, Percent_Identity=37.6923076923077, Blast_Score=303, Evalue=4e-82,
Organism=Homo sapiens, GI168229250, Length=520, Percent_Identity=37.6923076923077, Blast_Score=303, Evalue=4e-82,
Organism=Homo sapiens, GI296010852, Length=454, Percent_Identity=38.5462555066079, Blast_Score=273, Evalue=4e-73,
Organism=Homo sapiens, GI296010850, Length=454, Percent_Identity=38.5462555066079, Blast_Score=273, Evalue=4e-73,
Organism=Escherichia coli, GI1786889, Length=553, Percent_Identity=86.2567811934901, Blast_Score=1016, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147557, Length=569, Percent_Identity=43.2337434094903, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71993933, Length=560, Percent_Identity=43.2142857142857, Blast_Score=423, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17560178, Length=522, Percent_Identity=33.5249042145594, Blast_Score=281, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI25147560, Length=310, Percent_Identity=38.7096774193548, Blast_Score=191, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321563, Length=585, Percent_Identity=48.3760683760684, Blast_Score=530, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6325403, Length=586, Percent_Identity=47.2696245733788, Blast_Score=522, Evalue=1e-149,
Organism=Drosophila melanogaster, GI45553209, Length=576, Percent_Identity=45.1388888888889, Blast_Score=481, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 62142; Mature: 62142

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSIFGVLDLKTDPQALRKTALEMSRLMRHRGPDWSGIYAGENAILAHERLSIVDVNTGA
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEECCEEEEEECCCC
QPLYNAQRTHVLAVNGEIYNHQALRERLGDRYAFQTGSDCEVILALYQEYGPDFLDQLRG
CCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHH
MFAFILYDAERDAYLIGRDHLGIIPLYMGHDEHGNLFVASEMKALVPVCRTLREFPAGSA
HHEEEEEECCCCEEEEECCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCC
LWSRDGEIRRYYRRDWFDYAAVEHNPTDADVLRCALEESVKSHLMSDVPYGVLLSGGLDS
HHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCH
SIISAITKKFAARRVEDGERSAAWWPQLHSFAIGLQGAPDLKAAQEVANHLGTVHHEIHF
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHEEEE
TEQEGMDAIRDVIYHIETYDVTTIRASTPMYLMARKIKAMGIKMVLSGEGADEVFGGYLY
CHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCEEEEEECCCCHHHHCCEEE
FHKAPNAKEFHDETVRKLLALHMYDCARANKAMAAWGVEARVPFLDKTFLDVAMRLNPRD
EECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCC
KMCGNGRIEKQLLRECFESYLPASIAWRQKEQFSDGVGYSWIDSLKAAAAAQVSDQTLAN
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
ARFRFPYNTPTSKEAYLYRDIFEQLFPLPSAAECVPGGPSVACSSAKAIEWDESLRKMDD
CEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCC
PSGRAVGVHKSAY
CCCCEECCCCCCC
>Mature Secondary Structure
MCSIFGVLDLKTDPQALRKTALEMSRLMRHRGPDWSGIYAGENAILAHERLSIVDVNTGA
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEECCEEEEEECCCC
QPLYNAQRTHVLAVNGEIYNHQALRERLGDRYAFQTGSDCEVILALYQEYGPDFLDQLRG
CCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHH
MFAFILYDAERDAYLIGRDHLGIIPLYMGHDEHGNLFVASEMKALVPVCRTLREFPAGSA
HHEEEEEECCCCEEEEECCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCC
LWSRDGEIRRYYRRDWFDYAAVEHNPTDADVLRCALEESVKSHLMSDVPYGVLLSGGLDS
HHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCH
SIISAITKKFAARRVEDGERSAAWWPQLHSFAIGLQGAPDLKAAQEVANHLGTVHHEIHF
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHEEEE
TEQEGMDAIRDVIYHIETYDVTTIRASTPMYLMARKIKAMGIKMVLSGEGADEVFGGYLY
CHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCEEEEEECCCCHHHHCCEEE
FHKAPNAKEFHDETVRKLLALHMYDCARANKAMAAWGVEARVPFLDKTFLDVAMRLNPRD
EECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCC
KMCGNGRIEKQLLRECFESYLPASIAWRQKEQFSDGVGYSWIDSLKAAAAAQVSDQTLAN
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
ARFRFPYNTPTSKEAYLYRDIFEQLFPLPSAAECVPGGPSVACSSAKAIEWDESLRKMDD
CEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCC
PSGRAVGVHKSAY
CCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]