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The map label for this gene is ybfF [H]

Identifier: 238920776

GI number: 238920776

Start: 2787325

End: 2788113

Strand: Direct

Name: ybfF [H]

Synonym: NT01EI_2895

Alternate gene names: 238920776

Gene position: 2787325-2788113 (Clockwise)

Preceding gene: 238920773

Following gene: 238920777

Centisome position: 73.11

GC content: 66.54

Gene sequence:

>789_bases
ATGATCCTGACGCTGAATCACCTGCTGCTGCGGGCGCCGCAGCCGCCGAGCCAACCGCCACTGCTGCTGATCCACGGTCT
GTTCGGTTCTCTGGACAACCTGGGTCAGCTCGCCCGCTCGCTCAACCCCCAGCGCGACGTGCTGTTGGTCGATTTGCGCA
ACCACGGCCAGTCCCCCCATACCCCCGAGATGAACTATGATCTGCTCGTTGGGGATCTCATCGCGCTGATCGACGCCTAC
GGCCTGGCGCCGCTGGATATTATTGGCCACTCCATGGGTGGCAAAGCGGCGATGCGTCTGGCGGCGCTTGCTCCGCAGCG
CCTGCGCCGCCTGGTGGTGCTGGATATAGCGCCGGTGGCCTACCCCACCCGCCGTCACGACACCGTCTTTGCCGCCCTGA
ACGCAGTGACGGCGGCAGCGGCACAGACGCGCCCGCAGGCGGCGCAGATCCTGCGCCGCTTTCTGCCGCAGGAGGGAGTG
GTGCAGTTTCTGCTCAAATCCTTTCACCAGGGGCGCTGGCGCTTTAATCTGGCGGCCCTGCAGCAGCATTACCCACAGAT
CCTCGGCTGGCAGCCGCAGGGCCCCCATCAGGGGGCGACGCTGTTCATCAAGGGCGCCGACTCCCCCTATATTCTCGATG
ACTATCGCGGGGCCATAGCCCGCCAGTTTCCCCGCGCCCGCGCCCACGTGGTAAGCGGCAGCGGCCACTGGCTGCACGCC
GAACAGCCAGACAACGTCCTGCGTGCGGTGCGCCGCTTTCTCGATACGCCCCCCGACGCAGGGGCATAG

Upstream 100 bases:

>100_bases
CCGCCGAGCGCGGTGCGAACTCACGGGCACTCGGCCAGCGGACAATTCAATGTCGTCGTGGTAACCTGTGCGCAACATTA
GCTGAACAATTTATCTCGCC

Downstream 100 bases:

>100_bases
CGTAAAAAGCCGGGCTAAGCGGATTGTGCCATCGACGGTGTGGGAATGCGCACCGACGCCGATAAGGGATTGTCGCTGAT
CCACTCCCTGGGGTATGATG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MILTLNHLLLRAPQPPSQPPLLLIHGLFGSLDNLGQLARSLNPQRDVLLVDLRNHGQSPHTPEMNYDLLVGDLIALIDAY
GLAPLDIIGHSMGGKAAMRLAALAPQRLRRLVVLDIAPVAYPTRRHDTVFAALNAVTAAAAQTRPQAAQILRRFLPQEGV
VQFLLKSFHQGRWRFNLAALQQHYPQILGWQPQGPHQGATLFIKGADSPYILDDYRGAIARQFPRARAHVVSGSGHWLHA
EQPDNVLRAVRRFLDTPPDAGA

Sequences:

>Translated_262_residues
MILTLNHLLLRAPQPPSQPPLLLIHGLFGSLDNLGQLARSLNPQRDVLLVDLRNHGQSPHTPEMNYDLLVGDLIALIDAY
GLAPLDIIGHSMGGKAAMRLAALAPQRLRRLVVLDIAPVAYPTRRHDTVFAALNAVTAAAAQTRPQAAQILRRFLPQEGV
VQFLLKSFHQGRWRFNLAALQQHYPQILGWQPQGPHQGATLFIKGADSPYILDDYRGAIARQFPRARAHVVSGSGHWLHA
EQPDNVLRAVRRFLDTPPDAGA
>Mature_262_residues
MILTLNHLLLRAPQPPSQPPLLLIHGLFGSLDNLGQLARSLNPQRDVLLVDLRNHGQSPHTPEMNYDLLVGDLIALIDAY
GLAPLDIIGHSMGGKAAMRLAALAPQRLRRLVVLDIAPVAYPTRRHDTVFAALNAVTAAAAQTRPQAAQILRRFLPQEGV
VQFLLKSFHQGRWRFNLAALQQHYPQILGWQPQGPHQGATLFIKGADSPYILDDYRGAIARQFPRARAHVVSGSGHWLHA
EQPDNVLRAVRRFLDTPPDAGA

Specific function: Displays esterase activity toward palmitoyl-CoA, malonyl-CoA and pNP-butyrate [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200008, Length=248, Percent_Identity=40.3225806451613, Blast_Score=164, Evalue=9e-41,
Organism=Homo sapiens, GI23200012, Length=248, Percent_Identity=40.3225806451613, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI223941839, Length=239, Percent_Identity=34.3096234309623, Blast_Score=127, Evalue=8e-30,
Organism=Escherichia coli, GI1786902, Length=253, Percent_Identity=52.5691699604743, Blast_Score=271, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI17508535, Length=260, Percent_Identity=33.4615384615385, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17507063, Length=261, Percent_Identity=27.2030651340996, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6321468, Length=272, Percent_Identity=31.6176470588235, Blast_Score=114, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24640348, Length=259, Percent_Identity=29.3436293436293, Blast_Score=121, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24646055, Length=266, Percent_Identity=26.3157894736842, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 28892; Mature: 28892

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILTLNHLLLRAPQPPSQPPLLLIHGLFGSLDNLGQLARSLNPQRDVLLVDLRNHGQSPH
CEEEECHHHHCCCCCCCCCCEEEEEHHHCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCC
TPEMNYDLLVGDLIALIDAYGLAPLDIIGHSMGGKAAMRLAALAPQRLRRLVVLDIAPVA
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHEEECCCC
YPTRRHDTVFAALNAVTAAAAQTRPQAAQILRRFLPQEGVVQFLLKSFHQGRWRFNLAAL
CCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEHHHHH
QQHYPQILGWQPQGPHQGATLFIKGADSPYILDDYRGAIARQFPRARAHVVSGSGHWLHA
HHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEHHHHHHHHHHCCHHHEEEECCCCCEEEC
EQPDNVLRAVRRFLDTPPDAGA
CCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MILTLNHLLLRAPQPPSQPPLLLIHGLFGSLDNLGQLARSLNPQRDVLLVDLRNHGQSPH
CEEEECHHHHCCCCCCCCCCEEEEEHHHCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCC
TPEMNYDLLVGDLIALIDAYGLAPLDIIGHSMGGKAAMRLAALAPQRLRRLVVLDIAPVA
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHEEECCCC
YPTRRHDTVFAALNAVTAAAAQTRPQAAQILRRFLPQEGVVQFLLKSFHQGRWRFNLAAL
CCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEHHHHH
QQHYPQILGWQPQGPHQGATLFIKGADSPYILDDYRGAIARQFPRARAHVVSGSGHWLHA
HHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEHHHHHHHHHHCCHHHEEEECCCCCEEEC
EQPDNVLRAVRRFLDTPPDAGA
CCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]