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The map label for this gene is seqA [H]

Identifier: 238920775

GI number: 238920775

Start: 2786495

End: 2787034

Strand: Reverse

Name: seqA [H]

Synonym: NT01EI_2894

Alternate gene names: 238920775

Gene position: 2787034-2786495 (Counterclockwise)

Preceding gene: 238920781

Following gene: 238920774

Centisome position: 73.11

GC content: 60.56

Gene sequence:

>540_bases
ATGAAAACGATTGAAGTCGATGAGGAGCTCTACCGCTATATTGCCAGCCACACCCAGCGGATTGGCGAGAGTGCTTCCGA
CATTCTGCGCCGGCTGCTGAAAGTCGGTATGGAGAGCAAGACGGCCGCCGTCGCTGGCGCGGCGTTGCCGACGCTGGTGA
CTCCCCCCGCCACGGCGGTATCCCGGCCGCAGGATCGCGTGCGCACCATGCGTGAGCTGCTGTTGTCGGATGAGTATGCT
GAACAGAACAGGGCGGTCAGCCGCTTTATGCTGATCCTGACCACGCTGTACGCCATTGACGCCGATGGGTTTGCGGCGGC
AACGGATGCCCTGCACGGGCGCACCCGTGTCTACTTTGCCGGCGATCGCAATACGCTGCTGCAGAGTGGCAATCACACCA
AGCCCAAGCAGGTACCCGGTACCCCTTATTGGGTGATCACCAATACCAACTCAGGCCGCAAGCGCGCGATGGTCGAACAC
ATCATGCTGGCGATGCAGTTCCCCATGGAGCTGATCGAGAAGGTTTGCGGCACCATTTGA

Upstream 100 bases:

>100_bases
AATGGGCGTTAATCACCCTGCCGTGGCGCTAAACGCTATCCGCTCAGGGCGTTTAACCTTATAATCCAATCTGACCACGT
AGCAAAAGTACTGGATAACG

Downstream 100 bases:

>100_bases
TAAATAACAAGCGTTAGGGAGAAGCGCAGATGGCAAATCACCCGCGTGCCGGGCAGCCCGCCCGACAGAGCGATTTGATC
AATGTGGCTCAGCTGGCTGC

Product: SeqA protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MKTIEVDEELYRYIASHTQRIGESASDILRRLLKVGMESKTAAVAGAALPTLVTPPATAVSRPQDRVRTMRELLLSDEYA
EQNRAVSRFMLILTTLYAIDADGFAAATDALHGRTRVYFAGDRNTLLQSGNHTKPKQVPGTPYWVITNTNSGRKRAMVEH
IMLAMQFPMELIEKVCGTI

Sequences:

>Translated_179_residues
MKTIEVDEELYRYIASHTQRIGESASDILRRLLKVGMESKTAAVAGAALPTLVTPPATAVSRPQDRVRTMRELLLSDEYA
EQNRAVSRFMLILTTLYAIDADGFAAATDALHGRTRVYFAGDRNTLLQSGNHTKPKQVPGTPYWVITNTNSGRKRAMVEH
IMLAMQFPMELIEKVCGTI
>Mature_179_residues
MKTIEVDEELYRYIASHTQRIGESASDILRRLLKVGMESKTAAVAGAALPTLVTPPATAVSRPQDRVRTMRELLLSDEYA
EQNRAVSRFMLILTTLYAIDADGFAAATDALHGRTRVYFAGDRNTLLQSGNHTKPKQVPGTPYWVITNTNSGRKRAMVEH
IMLAMQFPMELIEKVCGTI

Specific function: Negative regulator of replication initiation. Required for sequestration, a process that blocks secondary initiation events. Could block the allosteric transition of dnaA from an inactive form to an active form. Binds hemimethylated oriC. This process has

COG id: COG3057

COG function: function code L; Negative regulator of replication initiationR

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786903, Length=183, Percent_Identity=67.7595628415301, Blast_Score=257, Evalue=4e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005621
- InterPro:   IPR010985 [H]

Pfam domain/function: PF03925 SeqA [H]

EC number: NA

Molecular weight: Translated: 19910; Mature: 19910

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIEVDEELYRYIASHTQRIGESASDILRRLLKVGMESKTAAVAGAALPTLVTPPATAV
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCCHHCCCCCHHH
SRPQDRVRTMRELLLSDEYAEQNRAVSRFMLILTTLYAIDADGFAAATDALHGRTRVYFA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEE
GDRNTLLQSGNHTKPKQVPGTPYWVITNTNSGRKRAMVEHIMLAMQFPMELIEKVCGTI
CCCHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTIEVDEELYRYIASHTQRIGESASDILRRLLKVGMESKTAAVAGAALPTLVTPPATAV
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCCHHCCCCCHHH
SRPQDRVRTMRELLLSDEYAEQNRAVSRFMLILTTLYAIDADGFAAATDALHGRTRVYFA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEE
GDRNTLLQSGNHTKPKQVPGTPYWVITNTNSGRKRAMVEHIMLAMQFPMELIEKVCGTI
CCCHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]