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The map label for this gene is phrB [H]

Identifier: 238920766

GI number: 238920766

Start: 2773018

End: 2774433

Strand: Reverse

Name: phrB [H]

Synonym: NT01EI_2885

Alternate gene names: 238920766

Gene position: 2774433-2773018 (Counterclockwise)

Preceding gene: 238920767

Following gene: 238920765

Centisome position: 72.78

GC content: 66.24

Gene sequence:

>1416_bases
GTGTGGCTGCGCAGCGATCTGCGCATGACGGATAACCGGGCGCTGCACGCGGCCTGCGCCGATGTCCGGGCCCGGGTGGT
GGCGCTGTATGTGGCGACACCGCGCCAGTGGCTGGCGCAGGAGATGGCGCCGCGTCAGGCGCAGTTTATCTGGCAGAATC
TGTTGGCGCTGCAGGTGACGCTGGGGCAGCGCCAGATCCCGCTGTATTGTCTGAGCGTGGACGACTATGCGGCCCAGGCG
CGGGCGGTGCGCGAGGTGTGCCTGCGTGAGCGTGCCGATGCGCTGTTTGTCAACCGGCAGTATGAGCTGAATGAGCGGCG
CCGCGACGATGCGCTGGCCGCCGCGCTGACCATCCCGTGCCATTTCTTTGACGACGCGCTGTTGCTGGCGCCGGGGACGG
TGCTGACCGGCGCGGGACAGATGTTTAAGGTCTTTACGCCGTTTAAACGCGCCTTTCTGCGCGGTCTGATGTCAGCCGAT
CTGCGCGTGCTACCCGCGCCGTCGCCGCGCCAGCCAGGCTCTGCGCCACCGGTGCAGGCCGAGCGCTTCAATTACCCGGA
TGCTGAGGTGGATAGCGCGCTGTTTCCAGCCGGTGAGGCGGCAGCCCAGACGCGTCTGGTGCAGTTTTGTCACGGAGCGC
TGGCGGCGTATGCGGCACGGCGCGATTTTCCTGCACAGGCGGGAACCAGCCAGCTCTCCCCTTACCTGACGTTGGGAGTG
TTATCGCCACGCCAGTGCCTGGCAGCGCTGCAGCCGCAGGCACTGGTGTCGCCGGGCCTGTCGAGCGGCGCTGAGGCCTG
GTTAAATGAGCTGATTTGGCGTGAGTTTTACCGCCACCTGCTGGTGGCCTGGCCGGATCTCTGTCGCCATCGTCCCTTTA
TTCCCTGGACGGCTCGGATCCGCTGGCGGGACGATCCGGCGGGACTGCTGCGCTGGCAGCAGGGGATGACAGGCTTTCCG
CTGGTGGATGCCGCCATGCGTCAGCTGAACCGCTGCGGCTGGATGCATAACCGGCTGCGCATGCTGACGGCCAGCTTTCT
GGTCAAAGATCTGCTTATCGACTGGCGCCTTGGCGAGCGTTATTTTCTGCGTCAGCTGCTCGACGGCGATCTGGCCGCGA
ACAACGGCGGCTGGCAGTGGGCCGCTTCAACGGGCAGCGATGCTGCGCCCTATTTCCGCATCTTTAATCCGCGTGTACAG
GGGCAGCGCTATGATCCGCAGGGGCAGTTTATCCGCCGGTGGCTCCCTGAACTGCGCGATGTCCCGGACAGTGACATTCA
TCACCCTCAGCGCTGGGCGCTGCGGCGGCAGCGCGTGCTAGACTATCCCGATCCGCAGGTCGATCACGCCCGCGCGCGGC
AGGACACGCTGTTGGCCTTTGCCCGCGCCCGCGCCGGGGACGACGGCGCGGCATGA

Upstream 100 bases:

>100_bases
ACCCGGACGACTATCTGGCATCGCAGCGCTATTTCTCCCCCTATCCGGACGCCCTGCGTCTGCGCTACGGTCATTAGGCG
GCGCGATGAGGACGCACCTG

Downstream 100 bases:

>100_bases
GGCGCAGGCGCCGCAGCGTGGCAGAGACAGGGAGAGTGGCAGATGATGCAGAGGATGTGGCGCAGATGGGTGGCCGGCGG
CGCGCTGCTGTGCGGCCTGA

Product: deoxyribodipyrimidine photo-lyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MWLRSDLRMTDNRALHAACADVRARVVALYVATPRQWLAQEMAPRQAQFIWQNLLALQVTLGQRQIPLYCLSVDDYAAQA
RAVREVCLRERADALFVNRQYELNERRRDDALAAALTIPCHFFDDALLLAPGTVLTGAGQMFKVFTPFKRAFLRGLMSAD
LRVLPAPSPRQPGSAPPVQAERFNYPDAEVDSALFPAGEAAAQTRLVQFCHGALAAYAARRDFPAQAGTSQLSPYLTLGV
LSPRQCLAALQPQALVSPGLSSGAEAWLNELIWREFYRHLLVAWPDLCRHRPFIPWTARIRWRDDPAGLLRWQQGMTGFP
LVDAAMRQLNRCGWMHNRLRMLTASFLVKDLLIDWRLGERYFLRQLLDGDLAANNGGWQWAASTGSDAAPYFRIFNPRVQ
GQRYDPQGQFIRRWLPELRDVPDSDIHHPQRWALRRQRVLDYPDPQVDHARARQDTLLAFARARAGDDGAA

Sequences:

>Translated_471_residues
MWLRSDLRMTDNRALHAACADVRARVVALYVATPRQWLAQEMAPRQAQFIWQNLLALQVTLGQRQIPLYCLSVDDYAAQA
RAVREVCLRERADALFVNRQYELNERRRDDALAAALTIPCHFFDDALLLAPGTVLTGAGQMFKVFTPFKRAFLRGLMSAD
LRVLPAPSPRQPGSAPPVQAERFNYPDAEVDSALFPAGEAAAQTRLVQFCHGALAAYAARRDFPAQAGTSQLSPYLTLGV
LSPRQCLAALQPQALVSPGLSSGAEAWLNELIWREFYRHLLVAWPDLCRHRPFIPWTARIRWRDDPAGLLRWQQGMTGFP
LVDAAMRQLNRCGWMHNRLRMLTASFLVKDLLIDWRLGERYFLRQLLDGDLAANNGGWQWAASTGSDAAPYFRIFNPRVQ
GQRYDPQGQFIRRWLPELRDVPDSDIHHPQRWALRRQRVLDYPDPQVDHARARQDTLLAFARARAGDDGAA
>Mature_471_residues
MWLRSDLRMTDNRALHAACADVRARVVALYVATPRQWLAQEMAPRQAQFIWQNLLALQVTLGQRQIPLYCLSVDDYAAQA
RAVREVCLRERADALFVNRQYELNERRRDDALAAALTIPCHFFDDALLLAPGTVLTGAGQMFKVFTPFKRAFLRGLMSAD
LRVLPAPSPRQPGSAPPVQAERFNYPDAEVDSALFPAGEAAAQTRLVQFCHGALAAYAARRDFPAQAGTSQLSPYLTLGV
LSPRQCLAALQPQALVSPGLSSGAEAWLNELIWREFYRHLLVAWPDLCRHRPFIPWTARIRWRDDPAGLLRWQQGMTGFP
LVDAAMRQLNRCGWMHNRLRMLTASFLVKDLLIDWRLGERYFLRQLLDGDLAANNGGWQWAASTGSDAAPYFRIFNPRVQ
GQRYDPQGQFIRRWLPELRDVPDSDIHHPQRWALRRQRVLDYPDPQVDHARARQDTLLAFARARAGDDGAA

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI4758072, Length=285, Percent_Identity=34.3859649122807, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI188536100, Length=285, Percent_Identity=32.6315789473684, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI188536103, Length=285, Percent_Identity=32.6315789473684, Blast_Score=129, Evalue=5e-30,
Organism=Escherichia coli, GI1786926, Length=470, Percent_Identity=55.7446808510638, Blast_Score=531, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6324962, Length=501, Percent_Identity=30.5389221556886, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI17137248, Length=282, Percent_Identity=34.0425531914894, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24585455, Length=282, Percent_Identity=34.0425531914894, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24648152, Length=258, Percent_Identity=30.6201550387597, Blast_Score=112, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 53457; Mature: 53457

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWLRSDLRMTDNRALHAACADVRARVVALYVATPRQWLAQEMAPRQAQFIWQNLLALQVT
CCCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHH
LGQRQIPLYCLSVDDYAAQARAVREVCLRERADALFVNRQYELNERRRDDALAAALTIPC
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHH
HFFDDALLLAPGTVLTGAGQMFKVFTPFKRAFLRGLMSADLRVLPAPSPRQPGSAPPVQA
HHHCCCHHHCCCHHEECCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCH
ERFNYPDAEVDSALFPAGEAAAQTRLVQFCHGALAAYAARRDFPAQAGTSQLSPYLTLGV
HHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEC
LSPRQCLAALQPQALVSPGLSSGAEAWLNELIWREFYRHLLVAWPDLCRHRPFIPWTARI
CCHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCEEEE
RWRDDPAGLLRWQQGMTGFPLVDAAMRQLNRCGWMHNRLRMLTASFLVKDLLIDWRLGER
EECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
YFLRQLLDGDLAANNGGWQWAASTGSDAAPYFRIFNPRVQGQRYDPQGQFIRRWLPELRD
HHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHC
VPDSDIHHPQRWALRRQRVLDYPDPQVDHARARQDTLLAFARARAGDDGAA
CCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MWLRSDLRMTDNRALHAACADVRARVVALYVATPRQWLAQEMAPRQAQFIWQNLLALQVT
CCCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHH
LGQRQIPLYCLSVDDYAAQARAVREVCLRERADALFVNRQYELNERRRDDALAAALTIPC
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHH
HFFDDALLLAPGTVLTGAGQMFKVFTPFKRAFLRGLMSADLRVLPAPSPRQPGSAPPVQA
HHHCCCHHHCCCHHEECCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCH
ERFNYPDAEVDSALFPAGEAAAQTRLVQFCHGALAAYAARRDFPAQAGTSQLSPYLTLGV
HHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEC
LSPRQCLAALQPQALVSPGLSSGAEAWLNELIWREFYRHLLVAWPDLCRHRPFIPWTARI
CCHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCEEEE
RWRDDPAGLLRWQQGMTGFPLVDAAMRQLNRCGWMHNRLRMLTASFLVKDLLIDWRLGER
EECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
YFLRQLLDGDLAANNGGWQWAASTGSDAAPYFRIFNPRVQGQRYDPQGQFIRRWLPELRD
HHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHC
VPDSDIHHPQRWALRRQRVLDYPDPQVDHARARQDTLLAFARARAGDDGAA
CCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6325460; 8905232; 9278503; 2200511; 7604260 [H]