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The map label for this gene is dusC [H]

Identifier: 238920710

GI number: 238920710

Start: 2714838

End: 2715794

Strand: Reverse

Name: dusC [H]

Synonym: NT01EI_2823

Alternate gene names: 238920710

Gene position: 2715794-2714838 (Counterclockwise)

Preceding gene: 238920711

Following gene: 238920707

Centisome position: 71.24

GC content: 63.74

Gene sequence:

>957_bases
ATGCTGAAAGTGAAACGGGTGCTGCTGGCGCCGATGGAGGGGGTGCTGGATGCCTTGGTGCGCGAGCTGCTCAGCGAGGT
GAACGACTACGATCTGTGCATCACCGAGTTTGTGCGGGTGGTGGACAGGCTGCTGCCGGAGAGGGTGTTCTACCGCCTGT
GTCCGGAGCTGTACCGTCGCAGTCAGACCCTGAGCGGCACGCCGGTGCGTATTCAGCTGCTGGGACAGTCGCCGCATTGG
CTGGCGGAGAACGCCCTGCGCGCGGTGACGCTGGGCTCGCCGGGGGTGGACTTCAACTGCGGTTGCCCTTCTAAGGCGGT
CAATGGCAGCGGCGGCGGTGCCACGCTGTTAAAAGATCCGGCGTTGATTTATGCCGGAGTGCAGGCGATGCGTCAGGCGA
TACCGGCACACTTGCCGCTGACGGTGAAGGTGCGTCTGGGCTGGGCGTGCGACAGCCAGCGTTTTGAAATCGCCGATGCG
GTACAGCAGGGTGGGGCGACGGAGCTGGTGGTACACGGTCGCACCAAAGAGGATGGCTACCGCGCCGATCGGATCAATTG
GGAGGCGATCGGCGAGATCCGTCGCCGTCTGCGGATCCCGGTGATCGCCAACGGCGAGATCTGGGACTACGCCAGCGCCC
AGGCGTGTCTGGCGCAGAGCGGGTGTGATGCGCTGATGATCGGGCGCGGCGCGCTGAATATCCCCAACCTGAGTCGGGTT
ATCAAAGCGAATGCACCGAAGATGCCGTGGCTGCAGGTGGTCACGCTGCTACAGCGCTACGTCCGGCTGGAGAAGCAGGG
AGATACCGGGCTGTATCACGTGGCGCGCATCAAGCAGTGGCTGAGCTATCTGCGTAAGGAGTATCCCCAGGCCGATGCCC
TGTTCCGTCAGATTCGCCCGCTGATGGATAGCCGCGCGATCGTAACGGCCATCGGCACGCTGGAGAGCGTCCGATAA

Upstream 100 bases:

>100_bases
GCGGCGACGGCGAGTGAATCCCTGTTGCGGACGGATGGGGAGCTCAGTACTCTCTGCGCCGGTCACGCATGGCGTGAGGG
GAGAATCTGAGGATAATGCG

Downstream 100 bases:

>100_bases
CGGGGTGGAGTATCAGGTCGGTCTCCCCGCCTCTTTGCCCCGCGAACCCAGGCGCGGTATCAGCCGAATATAAATCAGCT
CGCTCGCCAGTTCGACGACC

Product: tRNA-dihydrouridine synthase C

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MLKVKRVLLAPMEGVLDALVRELLSEVNDYDLCITEFVRVVDRLLPERVFYRLCPELYRRSQTLSGTPVRIQLLGQSPHW
LAENALRAVTLGSPGVDFNCGCPSKAVNGSGGGATLLKDPALIYAGVQAMRQAIPAHLPLTVKVRLGWACDSQRFEIADA
VQQGGATELVVHGRTKEDGYRADRINWEAIGEIRRRLRIPVIANGEIWDYASAQACLAQSGCDALMIGRGALNIPNLSRV
IKANAPKMPWLQVVTLLQRYVRLEKQGDTGLYHVARIKQWLSYLRKEYPQADALFRQIRPLMDSRAIVTAIGTLESVR

Sequences:

>Translated_318_residues
MLKVKRVLLAPMEGVLDALVRELLSEVNDYDLCITEFVRVVDRLLPERVFYRLCPELYRRSQTLSGTPVRIQLLGQSPHW
LAENALRAVTLGSPGVDFNCGCPSKAVNGSGGGATLLKDPALIYAGVQAMRQAIPAHLPLTVKVRLGWACDSQRFEIADA
VQQGGATELVVHGRTKEDGYRADRINWEAIGEIRRRLRIPVIANGEIWDYASAQACLAQSGCDALMIGRGALNIPNLSRV
IKANAPKMPWLQVVTLLQRYVRLEKQGDTGLYHVARIKQWLSYLRKEYPQADALFRQIRPLMDSRAIVTAIGTLESVR
>Mature_318_residues
MLKVKRVLLAPMEGVLDALVRELLSEVNDYDLCITEFVRVVDRLLPERVFYRLCPELYRRSQTLSGTPVRIQLLGQSPHW
LAENALRAVTLGSPGVDFNCGCPSKAVNGSGGGATLLKDPALIYAGVQAMRQAIPAHLPLTVKVRLGWACDSQRFEIADA
VQQGGATELVVHGRTKEDGYRADRINWEAIGEIRRRLRIPVIANGEIWDYASAQACLAQSGCDALMIGRGALNIPNLSRV
IKANAPKMPWLQVVTLLQRYVRLEKQGDTGLYHVARIKQWLSYLRKEYPQADALFRQIRPLMDSRAIVTAIGTLESVR

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=172, Percent_Identity=29.6511627906977, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI40807366, Length=243, Percent_Identity=28.3950617283951, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI239788483, Length=178, Percent_Identity=31.4606741573034, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI8923374, Length=287, Percent_Identity=26.8292682926829, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI239788462, Length=178, Percent_Identity=31.4606741573034, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1788462, Length=310, Percent_Identity=75.1612903225806, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI1789660, Length=317, Percent_Identity=29.6529968454259, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI145693211, Length=254, Percent_Identity=25.9842519685039, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25144369, Length=219, Percent_Identity=30.5936073059361, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323560, Length=202, Percent_Identity=26.7326732673267, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19921524, Length=169, Percent_Identity=30.1775147928994, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585320, Length=239, Percent_Identity=32.2175732217573, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24580595, Length=192, Percent_Identity=28.6458333333333, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI19920448, Length=192, Percent_Identity=28.6458333333333, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 35396; Mature: 35396

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVKRVLLAPMEGVLDALVRELLSEVNDYDLCITEFVRVVDRLLPERVFYRLCPELYRR
CCCHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SQTLSGTPVRIQLLGQSPHWLAENALRAVTLGSPGVDFNCGCPSKAVNGSGGGATLLKDP
CCCCCCCCEEEEEECCCCCHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHCCC
ALIYAGVQAMRQAIPAHLPLTVKVRLGWACDSQRFEIADAVQQGGATELVVHGRTKEDGY
HHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCCCCC
RADRINWEAIGEIRRRLRIPVIANGEIWDYASAQACLAQSGCDALMIGRGALNIPNLSRV
CCCCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHH
IKANAPKMPWLQVVTLLQRYVRLEKQGDTGLYHVARIKQWLSYLRKEYPQADALFRQIRP
HHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LMDSRAIVTAIGTLESVR
HHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLKVKRVLLAPMEGVLDALVRELLSEVNDYDLCITEFVRVVDRLLPERVFYRLCPELYRR
CCCHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SQTLSGTPVRIQLLGQSPHWLAENALRAVTLGSPGVDFNCGCPSKAVNGSGGGATLLKDP
CCCCCCCCEEEEEECCCCCHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHCCC
ALIYAGVQAMRQAIPAHLPLTVKVRLGWACDSQRFEIADAVQQGGATELVVHGRTKEDGY
HHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCCCCC
RADRINWEAIGEIRRRLRIPVIANGEIWDYASAQACLAQSGCDALMIGRGALNIPNLSRV
CCCCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHH
IKANAPKMPWLQVVTLLQRYVRLEKQGDTGLYHVARIKQWLSYLRKEYPQADALFRQIRP
HHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LMDSRAIVTAIGTLESVR
HHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]