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The map label for this gene is mltB [H]

Identifier: 238920674

GI number: 238920674

Start: 2679324

End: 2680412

Strand: Reverse

Name: mltB [H]

Synonym: NT01EI_2787

Alternate gene names: 238920674

Gene position: 2680412-2679324 (Counterclockwise)

Preceding gene: 238920679

Following gene: 238920673

Centisome position: 70.31

GC content: 64.28

Gene sequence:

>1089_bases
ATGCGTCAACTAATTGTGCTGCTCCCCCTGGTGGTAATGCTGAGCGCCTGTAGCAGCCATAAACCGAAAATCGCGGCACC
GGAGACGGCGGAGCAGGCGCTGAGCCGTAGCCAGTCGGGGGGATTTACGCTGGCGCCCGAGCACGCGCAGTATCAGCCGA
CCGGCGACTTTGTCCATAGCCCCGCCGCCGCCCAGTTTATCGATACGATGGTGCGTGAGCATGGATTCGAGCGCCGCCAG
CTGGAGACGGTGCTGTCGCAGACCCAGCGCCTGGACTATGTGCTGCGCCTGATGGATCGGCAGGCGCCGACCAGCCGCCC
GCCCGCCGGGCCAAACGGTGCCTGGCTGCGCTATCGCGACAAGTTTATTACGCCGGACAATATTCAGAACGGCGTGCAGT
TTTATAACCAGTATCATGCCGAACTGCTGCGGGCGCAGCAGATCTATGGCGTGCCGCCGGAGATCATCGTCGGCATCATC
GGGGTAGAGACCCGCTGGGGGCGGGTGATGGGCAAGACGCGCACTATTGATGCACTGGCCACGCTGGCCTTCGCCTATCC
GCGTCGCGCCGACTACTTCCGCGGCGAGCTGCAAACCTACCTGCTGATGGCGCGTGAGGAGGGTAAGGATCCGCTGACGC
TGCGCGGCTCGTTCGCCGGTGCCATGGGCTACGGCCAGTTTATGCCCTCGGCGTTTCAGGCCTATGCGGTCGACTTCAAC
GGCGACGGCCACGTGAACCTGTGGGACCCGGTGGACGCCATCGGCAGCGTGGCCAGCTATTTTAAAGGGCACGGCTGGAC
GCCTGGCGCCGCGGTCGCGGTGCAGGCCAACGGTCAGGCGCCGGGGCTGGATAACGGCTTTAAGACCCGCTACTCGGTGA
GCGAGCTGGCGCAGGCGGGGTTGTCACCCCGGAGCGCGCTGGAGGATGGGCATCAGGTCAGCCTGCTGCGCCTTGATGTC
GGCAGCGGTTACCAGTACTGGTACGGTCTGTCCAACTTCTACACCATTACCCGCTATAACCACAGCACCCACTACGCCAT
GGCCGTATGGCTGCTGGGCGAGTCGGTGGTCCGTTCCGCGGGACGCTGA

Upstream 100 bases:

>100_bases
TGCATGGCGCATCAGGGAACCCCTTGTAGGGATGTCTTCGTCGCGGGCAATGTTCTACCATAGTGATACCCTATTTTTGA
CCTCGTTAAATGGAAGTCCA

Downstream 100 bases:

>100_bases
TCCCGCCGTGTCCCCGCGCGGCGTCAGGGCCTGCCGCGGGGACGTTCTCTACGCTGTCCCGTTTTCTCGCCGCCGCGCCG
ATGCCTCGACGCTTGCGCTT

Product: murein hydrolase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MRQLIVLLPLVVMLSACSSHKPKIAAPETAEQALSRSQSGGFTLAPEHAQYQPTGDFVHSPAAAQFIDTMVREHGFERRQ
LETVLSQTQRLDYVLRLMDRQAPTSRPPAGPNGAWLRYRDKFITPDNIQNGVQFYNQYHAELLRAQQIYGVPPEIIVGII
GVETRWGRVMGKTRTIDALATLAFAYPRRADYFRGELQTYLLMAREEGKDPLTLRGSFAGAMGYGQFMPSAFQAYAVDFN
GDGHVNLWDPVDAIGSVASYFKGHGWTPGAAVAVQANGQAPGLDNGFKTRYSVSELAQAGLSPRSALEDGHQVSLLRLDV
GSGYQYWYGLSNFYTITRYNHSTHYAMAVWLLGESVVRSAGR

Sequences:

>Translated_362_residues
MRQLIVLLPLVVMLSACSSHKPKIAAPETAEQALSRSQSGGFTLAPEHAQYQPTGDFVHSPAAAQFIDTMVREHGFERRQ
LETVLSQTQRLDYVLRLMDRQAPTSRPPAGPNGAWLRYRDKFITPDNIQNGVQFYNQYHAELLRAQQIYGVPPEIIVGII
GVETRWGRVMGKTRTIDALATLAFAYPRRADYFRGELQTYLLMAREEGKDPLTLRGSFAGAMGYGQFMPSAFQAYAVDFN
GDGHVNLWDPVDAIGSVASYFKGHGWTPGAAVAVQANGQAPGLDNGFKTRYSVSELAQAGLSPRSALEDGHQVSLLRLDV
GSGYQYWYGLSNFYTITRYNHSTHYAMAVWLLGESVVRSAGR
>Mature_362_residues
MRQLIVLLPLVVMLSACSSHKPKIAAPETAEQALSRSQSGGFTLAPEHAQYQPTGDFVHSPAAAQFIDTMVREHGFERRQ
LETVLSQTQRLDYVLRLMDRQAPTSRPPAGPNGAWLRYRDKFITPDNIQNGVQFYNQYHAELLRAQQIYGVPPEIIVGII
GVETRWGRVMGKTRTIDALATLAFAYPRRADYFRGELQTYLLMAREEGKDPLTLRGSFAGAMGYGQFMPSAFQAYAVDFN
GDGHVNLWDPVDAIGSVASYFKGHGWTPGAAVAVQANGQAPGLDNGFKTRYSVSELAQAGLSPRSALEDGHQVSLLRLDV
GSGYQYWYGLSNFYTITRYNHSTHYAMAVWLLGESVVRSAGR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=357, Percent_Identity=70.0280112044818, Blast_Score=519, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 40086; Mature: 40086

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQLIVLLPLVVMLSACSSHKPKIAAPETAEQALSRSQSGGFTLAPEHAQYQPTGDFVHS
CCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHCC
PAAAQFIDTMVREHGFERRQLETVLSQTQRLDYVLRLMDRQAPTSRPPAGPNGAWLRYRD
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEC
KFITPDNIQNGVQFYNQYHAELLRAQQIYGVPPEIIVGIIGVETRWGRVMGKTRTIDALA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEECCCCCHHHHHHCCCHHHHHHH
TLAFAYPRRADYFRGELQTYLLMAREEGKDPLTLRGSFAGAMGYGQFMPSAFQAYAVDFN
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHCCHHHEEEEEEEC
GDGHVNLWDPVDAIGSVASYFKGHGWTPGAAVAVQANGQAPGLDNGFKTRYSVSELAQAG
CCCCEEECCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHCC
LSPRSALEDGHQVSLLRLDVGSGYQYWYGLSNFYTITRYNHSTHYAMAVWLLGESVVRSA
CCHHHHHCCCCEEEEEEEECCCCCCEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHC
GR
CC
>Mature Secondary Structure
MRQLIVLLPLVVMLSACSSHKPKIAAPETAEQALSRSQSGGFTLAPEHAQYQPTGDFVHS
CCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHCC
PAAAQFIDTMVREHGFERRQLETVLSQTQRLDYVLRLMDRQAPTSRPPAGPNGAWLRYRD
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEC
KFITPDNIQNGVQFYNQYHAELLRAQQIYGVPPEIIVGIIGVETRWGRVMGKTRTIDALA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEECCCCCHHHHHHCCCHHHHHHH
TLAFAYPRRADYFRGELQTYLLMAREEGKDPLTLRGSFAGAMGYGQFMPSAFQAYAVDFN
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHCCHHHEEEEEEEC
GDGHVNLWDPVDAIGSVASYFKGHGWTPGAAVAVQANGQAPGLDNGFKTRYSVSELAQAG
CCCCEEECCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHCC
LSPRSALEDGHQVSLLRLDVGSGYQYWYGLSNFYTITRYNHSTHYAMAVWLLGESVVRSA
CCHHHHHCCCCEEEEEEEECCCCCCEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHC
GR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]