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The map label for this gene is ackA [H]

Identifier: 238920582

GI number: 238920582

Start: 2588564

End: 2589763

Strand: Direct

Name: ackA [H]

Synonym: NT01EI_2694

Alternate gene names: 238920582

Gene position: 2588564-2589763 (Clockwise)

Preceding gene: 238920576

Following gene: 238920583

Centisome position: 67.9

GC content: 58.5

Gene sequence:

>1200_bases
ATGTCGAAGCTGGTACTGGTTCTTAACTGCGGCAGTTCTTCCCTGAAATTCGCCATCATCAACGCGGCAAACGGTGAAGA
GCACCTGTCTGGTTTAGCCGAATGTTTTAACCTCCCTGAAGCCCGTATTAAATGGAAAATGGACGGTGCAAAACACGAAG
CGGCGCTGGGCGCAGGCGCGGCTCATAGCGAAGCCTTAAGCTTCATCGTAAACACCATCCTGGCACAGAAGCCGGAGCTC
TCCGCCTCACTGACTGCCATCGGTCACCGTATCGTTCACGGCGGCGAGAAGTTCACCCACTCTGCTGTCATCAATGACGA
AGTTCTGCAGGGTATCAAAGACGCTATCCCGTTTGCACCACTGCACAACCCGGCGCACCTGATCGGCATCGAAGAAGCGT
TCAAATCCTTCCCGCTGCTGAAAGGCAAGAACGTCGCCGTCTTCGACACCGCATTCCACACCACCATGCCGGAAGAGTCC
TACCTGTATGCCCTGCCGTACCATCTGTACAAAGAGCACGGTATCCGCCGCTACGGCGCTCACGGCACCAGCCACTACTA
TGTCAGCCGCGAAGCAGCCAAAATGCTGAACAAGCCGGTTGAAGAGCTGAACGTGATCACCTGCCACCTGGGTAATGGCG
GCTCCGTCAGCGCCATCCGCAACGGCGAGTGCGTGGACACCTCTATGGGTCTGACTCCGCTGGAAGGACTGGTCATGGGC
ACCCGCTCCGGCGACATCGATCCGGCTATCGTGTTCCACCTGCACGATACCCTGGGCATGAGCGTAGCCGAAATCAACAC
CCTGCTGACCAAAGAATCCGGCCTGCTGGGCCTGACCGAAGTCACCAGCGACTGCCGCTACGTGGAAGACAACTACGACA
CCAAGGCTGACGCCAAGCGCGCCATGGACGTTTACTGCCACCGTCTGGCCAAATATGTTGGCGCCTATACCGCGCTGATG
GACGGCCGTCTGGACGCAGTGATCTTCACCGGCGGCATCGGTGAAAATGCCGCCATGGTGCGCGAGCTGACGCTGGACAA
ACTGGCGCTGCTGGGTCTGGAAATCGACCACCAACGTAACCTGGACGCCCGCTTCGGCAAATCTGGCGTGATCACCAAGG
ACGGCAGCCGCCTGGCCATGGTTATCCCGACCAACGAAGAGCTGGTTATCGCGCAGGACGCCTCCCGTCTGACCGCCTAA

Upstream 100 bases:

>100_bases
ACCGCGCATGATGTTAATCATAAATGCCGACAGTGTATTGCGCTACGCTTAGCGCCTGTCTGGTACATTTTCTAACCACG
CATAACAAGTAGGTACTTCC

Downstream 100 bases:

>100_bases
CCCGCCCACACCGCCTGCTCCGGCAGGCGGTGTTATTCGCCGAGGGCGCCCGTCGCCCCCGGTCGCTAAAGACTCCCTGT
CACCGCCCGGCCTCCCTGGG

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MSKLVLVLNCGSSSLKFAIINAANGEEHLSGLAECFNLPEARIKWKMDGAKHEAALGAGAAHSEALSFIVNTILAQKPEL
SASLTAIGHRIVHGGEKFTHSAVINDEVLQGIKDAIPFAPLHNPAHLIGIEEAFKSFPLLKGKNVAVFDTAFHTTMPEES
YLYALPYHLYKEHGIRRYGAHGTSHYYVSREAAKMLNKPVEELNVITCHLGNGGSVSAIRNGECVDTSMGLTPLEGLVMG
TRSGDIDPAIVFHLHDTLGMSVAEINTLLTKESGLLGLTEVTSDCRYVEDNYDTKADAKRAMDVYCHRLAKYVGAYTALM
DGRLDAVIFTGGIGENAAMVRELTLDKLALLGLEIDHQRNLDARFGKSGVITKDGSRLAMVIPTNEELVIAQDASRLTA

Sequences:

>Translated_399_residues
MSKLVLVLNCGSSSLKFAIINAANGEEHLSGLAECFNLPEARIKWKMDGAKHEAALGAGAAHSEALSFIVNTILAQKPEL
SASLTAIGHRIVHGGEKFTHSAVINDEVLQGIKDAIPFAPLHNPAHLIGIEEAFKSFPLLKGKNVAVFDTAFHTTMPEES
YLYALPYHLYKEHGIRRYGAHGTSHYYVSREAAKMLNKPVEELNVITCHLGNGGSVSAIRNGECVDTSMGLTPLEGLVMG
TRSGDIDPAIVFHLHDTLGMSVAEINTLLTKESGLLGLTEVTSDCRYVEDNYDTKADAKRAMDVYCHRLAKYVGAYTALM
DGRLDAVIFTGGIGENAAMVRELTLDKLALLGLEIDHQRNLDARFGKSGVITKDGSRLAMVIPTNEELVIAQDASRLTA
>Mature_398_residues
SKLVLVLNCGSSSLKFAIINAANGEEHLSGLAECFNLPEARIKWKMDGAKHEAALGAGAAHSEALSFIVNTILAQKPELS
ASLTAIGHRIVHGGEKFTHSAVINDEVLQGIKDAIPFAPLHNPAHLIGIEEAFKSFPLLKGKNVAVFDTAFHTTMPEESY
LYALPYHLYKEHGIRRYGAHGTSHYYVSREAAKMLNKPVEELNVITCHLGNGGSVSAIRNGECVDTSMGLTPLEGLVMGT
RSGDIDPAIVFHLHDTLGMSVAEINTLLTKESGLLGLTEVTSDCRYVEDNYDTKADAKRAMDVYCHRLAKYVGAYTALMD
GRLDAVIFTGGIGENAAMVRELTLDKLALLGLEIDHQRNLDARFGKSGVITKDGSRLAMVIPTNEELVIAQDASRLTA

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=398, Percent_Identity=87.6884422110553, Blast_Score=727, Evalue=0.0,
Organism=Escherichia coli, GI145693184, Length=396, Percent_Identity=44.6969696969697, Blast_Score=324, Evalue=8e-90,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43124; Mature: 42993

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLVLVLNCGSSSLKFAIINAANGEEHLSGLAECFNLPEARIKWKMDGAKHEAALGAGA
CCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHEEEEECCCCHHHHHCCCC
AHSEALSFIVNTILAQKPELSASLTAIGHRIVHGGEKFTHSAVINDEVLQGIKDAIPFAP
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHCCCCC
LHNPAHLIGIEEAFKSFPLLKGKNVAVFDTAFHTTMPEESYLYALPYHLYKEHGIRRYGA
CCCCHHEEEHHHHHHHCCCCCCCCEEEEECHHHCCCCCCCEEEECHHHHHHHCCCEECCC
HGTSHYYVSREAAKMLNKPVEELNVITCHLGNGGSVSAIRNGECVDTSMGLTPLEGLVMG
CCCCEEEECHHHHHHHHCCHHHCEEEEEEECCCCCEEEECCCCEEECCCCCCCCCCEEEE
TRSGDIDPAIVFHLHDTLGMSVAEINTLLTKESGLLGLTEVTSDCRYVEDNYDTKADAKR
CCCCCCCCEEEEEEHHHHCCHHHHHHHHHCCCCCCEEHHHHHCCCEEECCCCCCHHHHHH
AMDVYCHRLAKYVGAYTALMDGRLDAVIFTGGIGENAAMVRELTLDKLALLGLEIDHQRN
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCEEECCCCC
LDARFGKSGVITKDGSRLAMVIPTNEELVIAQDASRLTA
CCHHCCCCCCEEECCCEEEEEEECCCCEEEEECCHHCCC
>Mature Secondary Structure 
SKLVLVLNCGSSSLKFAIINAANGEEHLSGLAECFNLPEARIKWKMDGAKHEAALGAGA
CCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHEEEEECCCCHHHHHCCCC
AHSEALSFIVNTILAQKPELSASLTAIGHRIVHGGEKFTHSAVINDEVLQGIKDAIPFAP
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHCCCCC
LHNPAHLIGIEEAFKSFPLLKGKNVAVFDTAFHTTMPEESYLYALPYHLYKEHGIRRYGA
CCCCHHEEEHHHHHHHCCCCCCCCEEEEECHHHCCCCCCCEEEECHHHHHHHCCCEECCC
HGTSHYYVSREAAKMLNKPVEELNVITCHLGNGGSVSAIRNGECVDTSMGLTPLEGLVMG
CCCCEEEECHHHHHHHHCCHHHCEEEEEEECCCCCEEEECCCCEEECCCCCCCCCCEEEE
TRSGDIDPAIVFHLHDTLGMSVAEINTLLTKESGLLGLTEVTSDCRYVEDNYDTKADAKR
CCCCCCCCEEEEEEHHHHCCHHHHHHHHHCCCCCCEEHHHHHCCCEEECCCCCCHHHHHH
AMDVYCHRLAKYVGAYTALMDGRLDAVIFTGGIGENAAMVRELTLDKLALLGLEIDHQRN
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCEEECCCCC
LDARFGKSGVITKDGSRLAMVIPTNEELVIAQDASRLTA
CCHHCCCCCCEEECCCEEEEEEECCCCEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA