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The map label for this gene is yfbT [H]

Identifier: 238920578

GI number: 238920578

Start: 2586175

End: 2586825

Strand: Reverse

Name: yfbT [H]

Synonym: NT01EI_2690

Alternate gene names: 238920578

Gene position: 2586825-2586175 (Counterclockwise)

Preceding gene: 238920579

Following gene: 238920577

Centisome position: 67.85

GC content: 61.9

Gene sequence:

>651_bases
GTGAAGTGTAAAGGTTTTTTGTTTGACCTCGATGGAACCCTGGTGGATTCATTGCCGGTGGTTGAACGTTCGTGGATTAA
CTGGGCCAAGCGTCAGGGGATCAATCCTCAGCAAGTATTGGAATTTATTCATGGTAAGCAGGCAATCACCTCCCTGCGCC
ACTTTATGCCGGACGCCGATGAGGCGCGCCTGCAGGAGGAGTTTGACTGGCTGGAGGCGCTGGAGTCGCGCGACACCGAC
GGTATCGCCGCGCTGCCTGGTGCCGCCGCGCTGATTGCCCGTCTCGATGCGCTGGCGATCCCTTGGGGGATCGTGACCTC
GGGGTCGGTGCCGGTAGCCTACGCGCGCTGTAAGGCCGCCGGGCTGCCTAAGCCGGCGGTGTTTGTCACCGCAGAGCAGG
TGCAGCGTGGCAAGCCGGAGCCGGATGCCTATTTGCTTGGGGCACAAAGGCTGGGGCTGGCGCCGGGCGCCTGTGCGGTG
GTCGAGGATGCCCCGGCCGGGATCCTGGCCGGATTGACCGCGGGTTGTCAGGTGATCGCCGTCAATGCCCCGTCGGATAC
CCTCAAGCTGGATAAAGTCACGCTCAATCTGCACAGTCTGGCGCAAATCACGCTGACGCGCGTTGATGATGATGCCGAGA
TCACGCTGTAG

Upstream 100 bases:

>100_bases
CGTCTGGCATGCCTGTCCGCGCCAGTACCATTTAAGTGCCGTCGAGATTATGCAAATTTTGAATGCGTAACGGCGGTTGA
TTTTGTCATGAGGAGTACCC

Downstream 100 bases:

>100_bases
ACAATGTGTAAATGGAAAAGTCGATTAACCCCGCCTGTGCGGGGTTTTTTTATGGCATGATTCAGGATAACTAACCCGTT
TTTGTTATTCGTCGATGCCG

Product: phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MKCKGFLFDLDGTLVDSLPVVERSWINWAKRQGINPQQVLEFIHGKQAITSLRHFMPDADEARLQEEFDWLEALESRDTD
GIAALPGAAALIARLDALAIPWGIVTSGSVPVAYARCKAAGLPKPAVFVTAEQVQRGKPEPDAYLLGAQRLGLAPGACAV
VEDAPAGILAGLTAGCQVIAVNAPSDTLKLDKVTLNLHSLAQITLTRVDDDAEITL

Sequences:

>Translated_216_residues
MKCKGFLFDLDGTLVDSLPVVERSWINWAKRQGINPQQVLEFIHGKQAITSLRHFMPDADEARLQEEFDWLEALESRDTD
GIAALPGAAALIARLDALAIPWGIVTSGSVPVAYARCKAAGLPKPAVFVTAEQVQRGKPEPDAYLLGAQRLGLAPGACAV
VEDAPAGILAGLTAGCQVIAVNAPSDTLKLDKVTLNLHSLAQITLTRVDDDAEITL
>Mature_216_residues
MKCKGFLFDLDGTLVDSLPVVERSWINWAKRQGINPQQVLEFIHGKQAITSLRHFMPDADEARLQEEFDWLEALESRDTD
GIAALPGAAALIARLDALAIPWGIVTSGSVPVAYARCKAAGLPKPAVFVTAEQVQRGKPEPDAYLLGAQRLGLAPGACAV
VEDAPAGILAGLTAGCQVIAVNAPSDTLKLDKVTLNLHSLAQITLTRVDDDAEITL

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrate glucose 6-phosphate [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Homo sapiens, GI197382691, Length=208, Percent_Identity=32.6923076923077, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI87082080, Length=207, Percent_Identity=69.5652173913043, Blast_Score=279, Evalue=1e-76,
Organism=Escherichia coli, GI1787576, Length=195, Percent_Identity=30.2564102564103, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789046, Length=191, Percent_Identity=30.3664921465969, Blast_Score=63, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321834, Length=202, Percent_Identity=30.1980198019802, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321833, Length=202, Percent_Identity=29.2079207920792, Blast_Score=90, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320905, Length=197, Percent_Identity=32.48730964467, Blast_Score=78, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322136, Length=197, Percent_Identity=31.4720812182741, Blast_Score=75, Evalue=7e-15,
Organism=Drosophila melanogaster, GI20129151, Length=217, Percent_Identity=29.9539170506912, Blast_Score=74, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 23032; Mature: 23032

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCKGFLFDLDGTLVDSLPVVERSWINWAKRQGINPQQVLEFIHGKQAITSLRHFMPDAD
CCCCEEEEECCCCHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCH
EARLQEEFDWLEALESRDTDGIAALPGAAALIARLDALAIPWGIVTSGSVPVAYARCKAA
HHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHC
GLPKPAVFVTAEQVQRGKPEPDAYLLGAQRLGLAPGACAVVEDAPAGILAGLTAGCQVIA
CCCCCEEEEEHHHHHCCCCCCCEEEECHHHHCCCCCCEEEECCCCCHHHHHHCCCCEEEE
VNAPSDTLKLDKVTLNLHSLAQITLTRVDDDAEITL
EECCCCCEEEEEEEECHHHHEEEEEEEECCCCEECC
>Mature Secondary Structure
MKCKGFLFDLDGTLVDSLPVVERSWINWAKRQGINPQQVLEFIHGKQAITSLRHFMPDAD
CCCCEEEEECCCCHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCH
EARLQEEFDWLEALESRDTDGIAALPGAAALIARLDALAIPWGIVTSGSVPVAYARCKAA
HHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHC
GLPKPAVFVTAEQVQRGKPEPDAYLLGAQRLGLAPGACAVVEDAPAGILAGLTAGCQVIA
CCCCCEEEEEHHHHHCCCCCCCEEEECHHHHCCCCCCEEEECCCCCHHHHHHCCCCEEEE
VNAPSDTLKLDKVTLNLHSLAQITLTRVDDDAEITL
EECCCCCEEEEEEEECHHHHEEEEEEEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 10493123 [H]