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The map label for this gene is nrdA [H]

Identifier: 238920520

GI number: 238920520

Start: 2525544

End: 2527826

Strand: Direct

Name: nrdA [H]

Synonym: NT01EI_2632

Alternate gene names: 238920520

Gene position: 2525544-2527826 (Clockwise)

Preceding gene: 238920519

Following gene: 238920521

Centisome position: 66.25

GC content: 58.43

Gene sequence:

>2283_bases
ATGAATCAGAGTCTGCTCGTCACCAAACGCGACGGAAGCAAAGAACGCATCAATCTCGACAAAATCCACCGGGTTCTCGA
CTGGGCGGCGGAAGGGCTGCAAAACGTCTCCGTCTCCCAGGTTGAGCTGCGTTCCCACATCCAGTTTTATGACGGCATCC
GCACGGCGGACATCCATGAGACCATCATCAAAGCTGCCGCCGATCTGATCTCCCGTGAAACCCCGGACTACCAATACATG
GCCGCGCGCCTGGCGATCTTCCACCTGCGCAAAAAAGCCTATGGTCAGTTCGAGCCGCCCAAACTGTATGACCATGTCGT
ACGCCTGATAGAGATGGGCAAATATGACACTCACCTGCTGACCGACTTCAGCCGCGACGAGTTCGAGCAGATGGATAGCT
TTATCGACCACTGGCGCGATATGAACTTCTCCTATGCCGCGGTAAAGCAGCTGGAGGGCAAATACCTGGTACAGAACCGC
GTCAGCGGTGAGATCTATGAGAGCGCCCAGTTCCTCTATATCCTGGTCGCCGCCTGCCTGTTCTCCCACTACCCGCGCGA
GACCCGGCTGGACTATATCAAGCGCTTCTACGACGCGGTATCAACCTTTAAGATCTCTCTGCCGACGCCCATCATGTCCG
GTGTGCGTACTCCGACGCGTCAGTTCAGCTCCTGTGTGCTGATCGAGTGCGGCGACAGCCTGGATTCAATCAACGCCACC
TCCAGCGCCATTGTGAAGTATGTCTCCCAGCGCGCCGGCATCGGTATCAACGCTGGGCGCCTGCGTGCGTTGGGCAGCCC
GATCCGCGGAGGAGAGGCGTTCCATACCGGCTGTATCCCGTTCTACAAACACTTCCAGACCGCGGTCAAATCCTGCTCTC
AGGGCGGTGTACGCGGCGGTGCGGCAACCCTGTTTTACCCGCTGTGGCACCTGGAGGTAGAGAGCCTGCTGGTGCTGAAG
AATAACCGCGGGGTAGAGGATAACCGGGTTCGCCACATAGACTACGGCGTACAGCTCAACCGCCTGATGTACCAGCGTCT
GATCCAGAGCGGTGAAATCACCCTGTTCAGCCCCTCCGACGTTCCGGGGCTATACGACGCCTTCTTTGCCGATCAGAGCC
AGTTTGAGCGCCTTTATGTGCAGTACGAGCAGGACAACAGTATCCGCCAGCGCAAGATTAAGGCCGTAGATCTGTTCTCG
CTGATGATGCAGGAGCGCGCTTCGACCGGGCGTATCTACATCCAGAACGTCGATCACTGCAATACCCACAGCCCGTTTGA
TGCACACGTCGCGCCGGTACGCCAGTCCAACCTGTGTCTGGAGATCGCGCTGCCAACCAAACCGCTGGAAGATGTCAATG
ATGAAACCGGCGAGATAGCCCTGTGTACCCTGTCGGCCTTCAACCTGGGTGCCATCGACTCACTGGACGACCTGGAGGAG
CTGGCTACCCTGGCGGTCCGCGCGCTGGATGCCCTGCTCGACTACCAGGACTACCCGATCCCGGCCGCTAAGCGCGGCGC
CATGGGACGCCGCACCTTGGGTATCGGGGTGATCAACTTTGCCTACTACCTGGCGAAAAATGGGGTACGTTACTCCGATG
GCAGCGCCAATGCGCTGACCCATCGCACCTTTGAGGCGATCCAGTACTACCTGCTGAAGGCCTCCCACCGCCTGGCACAG
GAGCAGGGCGCCTGCCCGTGGTTCAACGAGACCACCTATGCGCAGGGGATCCTGCCCATCGACAGCTATAAGAAAGATCT
CGACGCTATCTGTAGCGAGCCGTTGCACTATGACTGGGAAACCCTGCGTACCGAGATCGTCCGCGACGGGCTGCGTAACT
CGACCCTGTCAGCCCTGATGCCGTCGGAGACCTCATCGCAAATCTCCAACGCCACCAACGGCATCGAGCCGCCGCGCGGC
TATATCAGTATCAAGGCCTCCAAGGACGGCATTCTGCGCCAGGTGGTGCCGGAGTATGAGCGCCTGAAGGATGCCTACGA
GCTGCTGTGGGCCATGCCGGGTAACGATGGCTATCTGCAGCTGGTCGGCCTGATGCAGAAGTTTATCGATCAATCGATCT
CAGCCAATACCAACTACGATCCGTCACGCTTCCCATCCGGGAAAGTACCGATGAAGCAGCTGTTAAAAGATCTGCTGACG
GCGTATAAACTGGGGGTGAAAACCCTCTATTATCAAAACACCCGCGACGGTGCGGAGGATATGCAGGAAGATCTGGGGAG
CGCGCAGGACGACGGCTGCGAAAGCGGCGCCTGCAAGATTTAA

Upstream 100 bases:

>100_bases
ATTCACCCCCTATATATTGTGTTATCATCGCGTCTACGCTACTAGCGTCAGCGGCGTTAACGCACGTCCAGCGGGGCTCA
CACGGACAGGAACGTATCCT

Downstream 100 bases:

>100_bases
CCTCCCCGCTGCACGGTGTCCCCGACGTGGGACGCCGCATCCGCGCGTTATTTTTGACGCCCCGCGCCAGCGGGGCACAA
CAGGATTAGGGTCATGGCCT

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Protein B1; Ribonucleoside-diphosphate reductase 1 R1 subunit; Ribonucleotide reductase 1 [H]

Number of amino acids: Translated: 760; Mature: 760

Protein sequence:

>760_residues
MNQSLLVTKRDGSKERINLDKIHRVLDWAAEGLQNVSVSQVELRSHIQFYDGIRTADIHETIIKAAADLISRETPDYQYM
AARLAIFHLRKKAYGQFEPPKLYDHVVRLIEMGKYDTHLLTDFSRDEFEQMDSFIDHWRDMNFSYAAVKQLEGKYLVQNR
VSGEIYESAQFLYILVAACLFSHYPRETRLDYIKRFYDAVSTFKISLPTPIMSGVRTPTRQFSSCVLIECGDSLDSINAT
SSAIVKYVSQRAGIGINAGRLRALGSPIRGGEAFHTGCIPFYKHFQTAVKSCSQGGVRGGAATLFYPLWHLEVESLLVLK
NNRGVEDNRVRHIDYGVQLNRLMYQRLIQSGEITLFSPSDVPGLYDAFFADQSQFERLYVQYEQDNSIRQRKIKAVDLFS
LMMQERASTGRIYIQNVDHCNTHSPFDAHVAPVRQSNLCLEIALPTKPLEDVNDETGEIALCTLSAFNLGAIDSLDDLEE
LATLAVRALDALLDYQDYPIPAAKRGAMGRRTLGIGVINFAYYLAKNGVRYSDGSANALTHRTFEAIQYYLLKASHRLAQ
EQGACPWFNETTYAQGILPIDSYKKDLDAICSEPLHYDWETLRTEIVRDGLRNSTLSALMPSETSSQISNATNGIEPPRG
YISIKASKDGILRQVVPEYERLKDAYELLWAMPGNDGYLQLVGLMQKFIDQSISANTNYDPSRFPSGKVPMKQLLKDLLT
AYKLGVKTLYYQNTRDGAEDMQEDLGSAQDDGCESGACKI

Sequences:

>Translated_760_residues
MNQSLLVTKRDGSKERINLDKIHRVLDWAAEGLQNVSVSQVELRSHIQFYDGIRTADIHETIIKAAADLISRETPDYQYM
AARLAIFHLRKKAYGQFEPPKLYDHVVRLIEMGKYDTHLLTDFSRDEFEQMDSFIDHWRDMNFSYAAVKQLEGKYLVQNR
VSGEIYESAQFLYILVAACLFSHYPRETRLDYIKRFYDAVSTFKISLPTPIMSGVRTPTRQFSSCVLIECGDSLDSINAT
SSAIVKYVSQRAGIGINAGRLRALGSPIRGGEAFHTGCIPFYKHFQTAVKSCSQGGVRGGAATLFYPLWHLEVESLLVLK
NNRGVEDNRVRHIDYGVQLNRLMYQRLIQSGEITLFSPSDVPGLYDAFFADQSQFERLYVQYEQDNSIRQRKIKAVDLFS
LMMQERASTGRIYIQNVDHCNTHSPFDAHVAPVRQSNLCLEIALPTKPLEDVNDETGEIALCTLSAFNLGAIDSLDDLEE
LATLAVRALDALLDYQDYPIPAAKRGAMGRRTLGIGVINFAYYLAKNGVRYSDGSANALTHRTFEAIQYYLLKASHRLAQ
EQGACPWFNETTYAQGILPIDSYKKDLDAICSEPLHYDWETLRTEIVRDGLRNSTLSALMPSETSSQISNATNGIEPPRG
YISIKASKDGILRQVVPEYERLKDAYELLWAMPGNDGYLQLVGLMQKFIDQSISANTNYDPSRFPSGKVPMKQLLKDLLT
AYKLGVKTLYYQNTRDGAEDMQEDLGSAQDDGCESGACKI
>Mature_760_residues
MNQSLLVTKRDGSKERINLDKIHRVLDWAAEGLQNVSVSQVELRSHIQFYDGIRTADIHETIIKAAADLISRETPDYQYM
AARLAIFHLRKKAYGQFEPPKLYDHVVRLIEMGKYDTHLLTDFSRDEFEQMDSFIDHWRDMNFSYAAVKQLEGKYLVQNR
VSGEIYESAQFLYILVAACLFSHYPRETRLDYIKRFYDAVSTFKISLPTPIMSGVRTPTRQFSSCVLIECGDSLDSINAT
SSAIVKYVSQRAGIGINAGRLRALGSPIRGGEAFHTGCIPFYKHFQTAVKSCSQGGVRGGAATLFYPLWHLEVESLLVLK
NNRGVEDNRVRHIDYGVQLNRLMYQRLIQSGEITLFSPSDVPGLYDAFFADQSQFERLYVQYEQDNSIRQRKIKAVDLFS
LMMQERASTGRIYIQNVDHCNTHSPFDAHVAPVRQSNLCLEIALPTKPLEDVNDETGEIALCTLSAFNLGAIDSLDDLEE
LATLAVRALDALLDYQDYPIPAAKRGAMGRRTLGIGVINFAYYLAKNGVRYSDGSANALTHRTFEAIQYYLLKASHRLAQ
EQGACPWFNETTYAQGILPIDSYKKDLDAICSEPLHYDWETLRTEIVRDGLRNSTLSALMPSETSSQISNATNGIEPPRG
YISIKASKDGILRQVVPEYERLKDAYELLWAMPGNDGYLQLVGLMQKFIDQSISANTNYDPSRFPSGKVPMKQLLKDLLT
AYKLGVKTLYYQNTRDGAEDMQEDLGSAQDDGCESGACKI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1 contains the binding sites for both substrates and allosteric effectors and carries out the actual reduction

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-cone domain [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=791, Percent_Identity=26.2958280657396, Blast_Score=231, Evalue=3e-60,
Organism=Escherichia coli, GI2367133, Length=761, Percent_Identity=88.173455978975, Blast_Score=1422, Evalue=0.0,
Organism=Escherichia coli, GI1789030, Length=579, Percent_Identity=26.2521588946459, Blast_Score=162, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17555242, Length=699, Percent_Identity=27.3247496423462, Blast_Score=222, Evalue=7e-58,
Organism=Saccharomyces cerevisiae, GI6322123, Length=654, Percent_Identity=29.0519877675841, Blast_Score=246, Evalue=9e-66,
Organism=Saccharomyces cerevisiae, GI6320914, Length=791, Percent_Identity=27.180783817952, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI17352481, Length=794, Percent_Identity=26.7002518891688, Blast_Score=246, Evalue=4e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005144
- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926 [H]

Pfam domain/function: PF03477 ATP-cone; PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 85741; Mature: 85741

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS51161 ATP_CONE ; PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQSLLVTKRDGSKERINLDKIHRVLDWAAEGLQNVSVSQVELRSHIQFYDGIRTADIHE
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHH
TIIKAAADLISRETPDYQYMAARLAIFHLRKKAYGQFEPPKLYDHVVRLIEMGKYDTHLL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHH
TDFSRDEFEQMDSFIDHWRDMNFSYAAVKQLEGKYLVQNRVSGEIYESAQFLYILVAACL
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHH
FSHYPRETRLDYIKRFYDAVSTFKISLPTPIMSGVRTPTRQFSSCVLIECGDSLDSINAT
HHCCCCHHHHHHHHHHHHHHHHEEEECCCHHHHCCCCCHHHHCCEEEEEECCCCCCCCCH
SSAIVKYVSQRAGIGINAGRLRALGSPIRGGEAFHTGCIPFYKHFQTAVKSCSQGGVRGG
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
AATLFYPLWHLEVESLLVLKNNRGVEDNRVRHIDYGVQLNRLMYQRLIQSGEITLFSPSD
CHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEECCCC
VPGLYDAFFADQSQFERLYVQYEQDNSIRQRKIKAVDLFSLMMQERASTGRIYIQNVDHC
CCHHHHHHHCCHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
NTHSPFDAHVAPVRQSNLCLEIALPTKPLEDVNDETGEIALCTLSAFNLGAIDSLDDLEE
CCCCCCCCCCCCCCCCCEEEEEECCCCCHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHH
LATLAVRALDALLDYQDYPIPAAKRGAMGRRTLGIGVINFAYYLAKNGVRYSDGSANALT
HHHHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCHHH
HRTFEAIQYYLLKASHRLAQEQGACPWFNETTYAQGILPIDSYKKDLDAICSEPLHYDWE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHCCCCCCCHH
TLRTEIVRDGLRNSTLSALMPSETSSQISNATNGIEPPRGYISIKASKDGILRQVVPEYE
HHHHHHHHHHHCCCHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHH
RLKDAYELLWAMPGNDGYLQLVGLMQKFIDQSISANTNYDPSRFPSGKVPMKQLLKDLLT
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AYKLGVKTLYYQNTRDGAEDMQEDLGSAQDDGCESGACKI
HHHHHHHHEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNQSLLVTKRDGSKERINLDKIHRVLDWAAEGLQNVSVSQVELRSHIQFYDGIRTADIHE
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHH
TIIKAAADLISRETPDYQYMAARLAIFHLRKKAYGQFEPPKLYDHVVRLIEMGKYDTHLL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHH
TDFSRDEFEQMDSFIDHWRDMNFSYAAVKQLEGKYLVQNRVSGEIYESAQFLYILVAACL
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHH
FSHYPRETRLDYIKRFYDAVSTFKISLPTPIMSGVRTPTRQFSSCVLIECGDSLDSINAT
HHCCCCHHHHHHHHHHHHHHHHEEEECCCHHHHCCCCCHHHHCCEEEEEECCCCCCCCCH
SSAIVKYVSQRAGIGINAGRLRALGSPIRGGEAFHTGCIPFYKHFQTAVKSCSQGGVRGG
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
AATLFYPLWHLEVESLLVLKNNRGVEDNRVRHIDYGVQLNRLMYQRLIQSGEITLFSPSD
CHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEECCCC
VPGLYDAFFADQSQFERLYVQYEQDNSIRQRKIKAVDLFSLMMQERASTGRIYIQNVDHC
CCHHHHHHHCCHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
NTHSPFDAHVAPVRQSNLCLEIALPTKPLEDVNDETGEIALCTLSAFNLGAIDSLDDLEE
CCCCCCCCCCCCCCCCCEEEEEECCCCCHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHH
LATLAVRALDALLDYQDYPIPAAKRGAMGRRTLGIGVINFAYYLAKNGVRYSDGSANALT
HHHHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCHHH
HRTFEAIQYYLLKASHRLAQEQGACPWFNETTYAQGILPIDSYKKDLDAICSEPLHYDWE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHCCCCCCCHH
TLRTEIVRDGLRNSTLSALMPSETSSQISNATNGIEPPRGYISIKASKDGILRQVVPEYE
HHHHHHHHHHHCCCHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHH
RLKDAYELLWAMPGNDGYLQLVGLMQKFIDQSISANTNYDPSRFPSGKVPMKQLLKDLLT
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AYKLGVKTLYYQNTRDGAEDMQEDLGSAQDDGCESGACKI
HHHHHHHHEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6087316; 3287341; 9205837; 9278503; 3894026; 2663852; 8702814; 8052308; 9309223; 10493864 [H]