Click here to switch to the map view.

The map label for this gene is fruA [H]

Identifier: 238920479

GI number: 238920479

Start: 2484181

End: 2485878

Strand: Reverse

Name: fruA [H]

Synonym: NT01EI_2590

Alternate gene names: 238920479

Gene position: 2485878-2484181 (Counterclockwise)

Preceding gene: 238920480

Following gene: 238920474

Centisome position: 65.21

GC content: 65.49

Gene sequence:

>1698_bases
ATGAATACCCTACTGATTACGGAAGGTGCGCAGGGCGAGGCCCGCTGTTTCCTTGCCAGGCGTATGCTGGAGGCCGTCGC
CGCCCAGCAGCAGATTACCTGGGTTACCCGCCCGCAGGATGCGGCGCTGGTGCTGTTTATCGGTGACGATACCCCGCAGG
ACGCCGCGCTGGAGGGGAAACCGCTGTACCGGGGCTCGGTGGGCGAAGCCATTCGCCAGCCGGCGGAGTTGCTGGCCCGC
GCGCTGCGCGATGCCTCGCCATATCACTGTGCGGCGCCGCGACAGGATCGATCGCAATGCCTGCGGCTGGTCGCGGTTAC
CGCCTGTCCGACGGGGGTTGCGCATACCTTCATGGCGGCGGAGGCGCTGGAGGCAGAGGCGCGCCGTCGCGGCTGGCAGG
TAAAGGTGGAGACGCGGGGATCGGTAGGGGCAGGCAACGCTATCACGCCGCAGGAGGTCGCGGAGGCCGATCTGGTGGTG
GTGGCGGCGGATATTGAGGTGGATCTGGCAAAGTTCGCCGGTAAACCGATGTACCGCACCAGCACAGGCCTGGCGCTGAA
GAAGACGTCTCAGGAGCTGGACAGGGCGCTGCAGGAAGCGGAGATATATCGCCCTCAGACATCGGCGCCGTCCGGGGCCG
CGGCATCGGGGCGCCGGAAAGAGGCGGGCGGTGCGTACCGTCACCTGCTGACCGGCGTCTCCTACATGTTGCCGATGGTG
GTGGCCGGCGGCCTGTGTATCGCGCTTTCGTTCGTCTTTGGTATTCAGGCCTTCAAGGAGCCTGGCACATTGGCGGCCGC
GCTGATGCAGATCGGCGGCGGCACCGCTTTTGCGTTGATGGTGCCGGTGCTGGCGGGTTATATCGCCTTCTCCATCGCCG
ATCGCCCCGGTCTGACGCCGGGACTGATCGGCGGGATGCTGGCGGTTAGCCTGGGCTCGGGCTTTATCGGCGGGATCATC
GCCGGCTTTCTCGCCGGCTATGTTGCCCGGGCCATCAGCAGCAGGCTGACTCTGCCTGCGAGCATGGAGGCACTTAAGCC
GATCCTGATCATTCCGCTGCTGTCGAGCCTGATCGTCGGCCTGGCGATGATTTACATCATCGGTACGCCGGTGGCGAAGC
TCCTGGCCTGGCTGACCCACTGGCTGCAGACCATGGGCACGGCTAATGCGGTGATCCTGGGGGCGGTACTTGGCGCGATG
ATGTGTACCGACATGGGCGGACCGGTGAATAAGGCGGCCTATGCTTTCGGTGTAGGTCTGCTCAGTACCCAGACTTACGG
TCCCATGGCGGCCATCATGGCCGCCGGGATGGTTCCGCCGCTGGCGATGGGGCTGGCGACGCTGGTCGCGCGCCGCAAGT
TTGCCCAGAGCGAGCGTGAGGGCGGTAAGGCGGCCGTGGTGCTGGGGCTGTGCTTCATCACCGAAGGGGCGATCCCCTTT
GCCGCACGGGATCCGATGCGGGTACTGCCGTGCTGCATTTTGGGCGGGGCGCTGACCGGGGCGCTGTCGATGCTGTTTGG
CGCGAAGCTGATGGCGCCGCACGGCGGACTGTTTGTTCTGCTGATCCCCGGCGCGATCAGCCCGGTAGTGCCTTATCTGC
TGGCGATCTTGGCCGGAACGCTGCTGAGTGGGCTGTTGTATGCGCTGCTCAAGCGTCCAGATGTTGCACCACAGGAGCGG
GTGACGAAAACGGCATAG

Upstream 100 bases:

>100_bases
CGGTCAGTCAGAGCAATGTCGGCATCAGCGACCGCACGCAGCTGGCGGCGATGATGGCACGCGTGGAGCTCGATCCTTTT
AACTGATTGTGGAAGGTCAT

Downstream 100 bases:

>100_bases
TCATTTGAATGATAGCGTGTGGGGGAGCCGGGAGGCTCCCCTATTTTTTTAGGCGGCCTCGTCACAGGAAAGCGCCGGGG
GACGCTGGGTGAAACGCCAG

Product: PTS system fructose-specific transporter subunits IIBC

Products: NA

Alternate protein names: EIIBC-Fru; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MNTLLITEGAQGEARCFLARRMLEAVAAQQQITWVTRPQDAALVLFIGDDTPQDAALEGKPLYRGSVGEAIRQPAELLAR
ALRDASPYHCAAPRQDRSQCLRLVAVTACPTGVAHTFMAAEALEAEARRRGWQVKVETRGSVGAGNAITPQEVAEADLVV
VAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDRALQEAEIYRPQTSAPSGAAASGRRKEAGGAYRHLLTGVSYMLPMV
VAGGLCIALSFVFGIQAFKEPGTLAAALMQIGGGTAFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSLGSGFIGGII
AGFLAGYVARAISSRLTLPASMEALKPILIIPLLSSLIVGLAMIYIIGTPVAKLLAWLTHWLQTMGTANAVILGAVLGAM
MCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATLVARRKFAQSEREGGKAAVVLGLCFITEGAIPF
AARDPMRVLPCCILGGALTGALSMLFGAKLMAPHGGLFVLLIPGAISPVVPYLLAILAGTLLSGLLYALLKRPDVAPQER
VTKTA

Sequences:

>Translated_565_residues
MNTLLITEGAQGEARCFLARRMLEAVAAQQQITWVTRPQDAALVLFIGDDTPQDAALEGKPLYRGSVGEAIRQPAELLAR
ALRDASPYHCAAPRQDRSQCLRLVAVTACPTGVAHTFMAAEALEAEARRRGWQVKVETRGSVGAGNAITPQEVAEADLVV
VAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDRALQEAEIYRPQTSAPSGAAASGRRKEAGGAYRHLLTGVSYMLPMV
VAGGLCIALSFVFGIQAFKEPGTLAAALMQIGGGTAFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSLGSGFIGGII
AGFLAGYVARAISSRLTLPASMEALKPILIIPLLSSLIVGLAMIYIIGTPVAKLLAWLTHWLQTMGTANAVILGAVLGAM
MCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATLVARRKFAQSEREGGKAAVVLGLCFITEGAIPF
AARDPMRVLPCCILGGALTGALSMLFGAKLMAPHGGLFVLLIPGAISPVVPYLLAILAGTLLSGLLYALLKRPDVAPQER
VTKTA
>Mature_565_residues
MNTLLITEGAQGEARCFLARRMLEAVAAQQQITWVTRPQDAALVLFIGDDTPQDAALEGKPLYRGSVGEAIRQPAELLAR
ALRDASPYHCAAPRQDRSQCLRLVAVTACPTGVAHTFMAAEALEAEARRRGWQVKVETRGSVGAGNAITPQEVAEADLVV
VAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDRALQEAEIYRPQTSAPSGAAASGRRKEAGGAYRHLLTGVSYMLPMV
VAGGLCIALSFVFGIQAFKEPGTLAAALMQIGGGTAFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSLGSGFIGGII
AGFLAGYVARAISSRLTLPASMEALKPILIIPLLSSLIVGLAMIYIIGTPVAKLLAWLTHWLQTMGTANAVILGAVLGAM
MCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATLVARRKFAQSEREGGKAAVVLGLCFITEGAIPF
AARDPMRVLPCCILGGALTGALSMLFGAKLMAPHGGLFVLLIPGAISPVVPYLLAILAGTLLSGLLYALLKRPDVAPQER
VTKTA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=558, Percent_Identity=73.8351254480287, Blast_Score=817, Evalue=0.0,
Organism=Escherichia coli, GI1786951, Length=501, Percent_Identity=34.9301397205589, Blast_Score=262, Evalue=5e-71,
Organism=Escherichia coli, GI87082348, Length=489, Percent_Identity=33.3333333333333, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1790386, Length=300, Percent_Identity=43, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1788729, Length=403, Percent_Identity=28.0397022332506, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1790387, Length=108, Percent_Identity=45.3703703703704, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788730, Length=91, Percent_Identity=41.7582417582418, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790390, Length=65, Percent_Identity=46.1538461538462, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 58748; Mature: 58748

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLLITEGAQGEARCFLARRMLEAVAAQQQITWVTRPQDAALVLFIGDDTPQDAALEGK
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCCCCCCCCC
PLYRGSVGEAIRQPAELLARALRDASPYHCAAPRQDRSQCLRLVAVTACPTGVAHTFMAA
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
EALEAEARRRGWQVKVETRGSVGAGNAITPQEVAEADLVVVAADIEVDLAKFAGKPMYRT
HHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHCCCCEEEEEECCHHHHHHHCCCCCEEC
STGLALKKTSQELDRALQEAEIYRPQTSAPSGAAASGRRKEAGGAYRHLLTGVSYMLPMV
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VAGGLCIALSFVFGIQAFKEPGTLAAALMQIGGGTAFALMVPVLAGYIAFSIADRPGLTP
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCH
GLIGGMLAVSLGSGFIGGIIAGFLAGYVARAISSRLTLPASMEALKPILIIPLLSSLIVG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LAMIYIIGTPVAKLLAWLTHWLQTMGTANAVILGAVLGAMMCTDMGGPVNKAAYAFGVGL
HHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LSTQTYGPMAAIMAAGMVPPLAMGLATLVARRKFAQSEREGGKAAVVLGLCFITEGAIPF
HCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCC
AARDPMRVLPCCILGGALTGALSMLFGAKLMAPHGGLFVLLIPGAISPVVPYLLAILAGT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHH
LLSGLLYALLKRPDVAPQERVTKTA
HHHHHHHHHHHCCCCCCHHHHCCCC
>Mature Secondary Structure
MNTLLITEGAQGEARCFLARRMLEAVAAQQQITWVTRPQDAALVLFIGDDTPQDAALEGK
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCCCCCCCCC
PLYRGSVGEAIRQPAELLARALRDASPYHCAAPRQDRSQCLRLVAVTACPTGVAHTFMAA
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
EALEAEARRRGWQVKVETRGSVGAGNAITPQEVAEADLVVVAADIEVDLAKFAGKPMYRT
HHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHCCCCEEEEEECCHHHHHHHCCCCCEEC
STGLALKKTSQELDRALQEAEIYRPQTSAPSGAAASGRRKEAGGAYRHLLTGVSYMLPMV
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VAGGLCIALSFVFGIQAFKEPGTLAAALMQIGGGTAFALMVPVLAGYIAFSIADRPGLTP
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCH
GLIGGMLAVSLGSGFIGGIIAGFLAGYVARAISSRLTLPASMEALKPILIIPLLSSLIVG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LAMIYIIGTPVAKLLAWLTHWLQTMGTANAVILGAVLGAMMCTDMGGPVNKAAYAFGVGL
HHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LSTQTYGPMAAIMAAGMVPPLAMGLATLVARRKFAQSEREGGKAAVVLGLCFITEGAIPF
HCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCC
AARDPMRVLPCCILGGALTGALSMLFGAKLMAPHGGLFVLLIPGAISPVVPYLLAILAGT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHH
LLSGLLYALLKRPDVAPQERVTKTA
HHHHHHHHHHHCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3076173; 9278503; 9097040; 1981619 [H]