Click here to switch to the map view.

The map label for this gene is rimO [H]

Identifier: 238920449

GI number: 238920449

Start: 2453083

End: 2454408

Strand: Direct

Name: rimO [H]

Synonym: NT01EI_2560

Alternate gene names: 238920449

Gene position: 2453083-2454408 (Clockwise)

Preceding gene: 238920448

Following gene: 238920450

Centisome position: 64.35

GC content: 61.24

Gene sequence:

>1326_bases
ATGAGCAATCAATCCCGCCAACCGACAATCGGTTTCGTCTCCCTCGGCTGCCCGAAAAACCTGGTCGACTCCGAACGTAT
CCTGACCGAGCTGCGCACCGAAGGCTACCTGGTGGTGCCTACCTATGAAGATGCCGATCTGGTGATCGTTAACACCTGCG
GCTTTATCGACAGCGCGGTACAGGAGTCTCTGGAGGCCATCGGCGAGGCGCTGCATGAAAACGGGAAAGTCATCGTGACT
GGCTGCCTGGGCGCCAAAGAGAACCAGATCCGGGAGGTCCACCCCAAGGTGCTGGAGATCTCCGGGCCGCACAGCTATGA
GCAGGTGCTGCACCACGTGCACAAATACGTGGCCAAACCGCAGCACAACCCTTTCACCAGCCTGGTTCCGGAACAGGGCG
TCAAGCTAACACCCAAGCATTTCGCCTATCTGAAAATCTCGGAGGGCTGTAACCACCGCTGCGCGTTCTGCATTATCCCC
TCCCTGCGCGGCGATCTGGAGAGCCGCTCCATCGGCTCTGTACTGGACGAGGCCAAGCGTCTGGCCGACTCCGGCGTCCG
TGAGCTGCTGGTCATCTCGCAGGATACCGCTGCCTACGGCGCGGATATCCAGCACCGCACCGGCTTTTGGAACGGTCAGC
CGGTCAAAACCAGCATGCTCAGCCTGTGTGAACAGCTGGCGACGCTGGGAGTCTGGATCCGCCTGCACTATGTTTACCCC
TACCCGCACGTGGATGACGTTATCCCGCTGATGGCACAGGGCAAGCTGCTGCCCTATCTGGATATCCCGCTGCAGCACGC
CAGCCCACGTATTCTGAAGCTGATGAAGCGCCCCGGCGCCGTTGAGCGCACGTTGGAGCGGATCAAACGCTGGCGCGAGA
TCTGCCCGCAGCTGACCCTGCGTTCGACCTTTATCGTCGGCTTCCCCGGCGAGACGGAAGAGGATTTCCAGATGCTGCTG
GACTTCCTGCGCGAGGCTCGCCTGGATCGCGTCGGCTGCTTCCAGTACAGCCCGGTTGAAGGCGCCGCCGCCAACGAACT
GCCCGATCAGGTACCCGACGAGATCAAGCAGGCGCGCTTCGATCGCTTTATGCAGCTGCAGCAGCAGATCTCCGCCGAGC
GGCTGCAAGAGAAGGTGGGGCTGACGCTGCCGGTGATCATCGACGAGGTCGATGGGGAAGGCGCCATAGGCCGCAGCATG
GCCGATGCGCCGGAGATCGACGGCGTGGTCTATCTGAATGGCGAGCGCAGCGTGAAACCGGGCGACATCGTCAATGTCAC
CATCGAAAATGCCGATGAGTACGACATGTGGGGCTCTCTGGCCTAA

Upstream 100 bases:

>100_bases
GCGTACGTCGAGCCATCAGGATAAATAGCGGTACAATTTCAACGCAGAGCGCTGTAAAATCCCTGCCCTATCATAACCAG
ACACGATGAATTTTTACGCT

Downstream 100 bases:

>100_bases
TGTCCGTGGCGCACCGTGCCTGCGGTGTGCCGTTTCAGCCACATAAGCTGACTGCCCCTTCTCTCATTAACCCTATCAAA
AAATCTTTTTGCTGGCGTTC

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSNQSRQPTIGFVSLGCPKNLVDSERILTELRTEGYLVVPTYEDADLVIVNTCGFIDSAVQESLEAIGEALHENGKVIVT
GCLGAKENQIREVHPKVLEISGPHSYEQVLHHVHKYVAKPQHNPFTSLVPEQGVKLTPKHFAYLKISEGCNHRCAFCIIP
SLRGDLESRSIGSVLDEAKRLADSGVRELLVISQDTAAYGADIQHRTGFWNGQPVKTSMLSLCEQLATLGVWIRLHYVYP
YPHVDDVIPLMAQGKLLPYLDIPLQHASPRILKLMKRPGAVERTLERIKRWREICPQLTLRSTFIVGFPGETEEDFQMLL
DFLREARLDRVGCFQYSPVEGAAANELPDQVPDEIKQARFDRFMQLQQQISAERLQEKVGLTLPVIIDEVDGEGAIGRSM
ADAPEIDGVVYLNGERSVKPGDIVNVTIENADEYDMWGSLA

Sequences:

>Translated_441_residues
MSNQSRQPTIGFVSLGCPKNLVDSERILTELRTEGYLVVPTYEDADLVIVNTCGFIDSAVQESLEAIGEALHENGKVIVT
GCLGAKENQIREVHPKVLEISGPHSYEQVLHHVHKYVAKPQHNPFTSLVPEQGVKLTPKHFAYLKISEGCNHRCAFCIIP
SLRGDLESRSIGSVLDEAKRLADSGVRELLVISQDTAAYGADIQHRTGFWNGQPVKTSMLSLCEQLATLGVWIRLHYVYP
YPHVDDVIPLMAQGKLLPYLDIPLQHASPRILKLMKRPGAVERTLERIKRWREICPQLTLRSTFIVGFPGETEEDFQMLL
DFLREARLDRVGCFQYSPVEGAAANELPDQVPDEIKQARFDRFMQLQQQISAERLQEKVGLTLPVIIDEVDGEGAIGRSM
ADAPEIDGVVYLNGERSVKPGDIVNVTIENADEYDMWGSLA
>Mature_440_residues
SNQSRQPTIGFVSLGCPKNLVDSERILTELRTEGYLVVPTYEDADLVIVNTCGFIDSAVQESLEAIGEALHENGKVIVTG
CLGAKENQIREVHPKVLEISGPHSYEQVLHHVHKYVAKPQHNPFTSLVPEQGVKLTPKHFAYLKISEGCNHRCAFCIIPS
LRGDLESRSIGSVLDEAKRLADSGVRELLVISQDTAAYGADIQHRTGFWNGQPVKTSMLSLCEQLATLGVWIRLHYVYPY
PHVDDVIPLMAQGKLLPYLDIPLQHASPRILKLMKRPGAVERTLERIKRWREICPQLTLRSTFIVGFPGETEEDFQMLLD
FLREARLDRVGCFQYSPVEGAAANELPDQVPDEIKQARFDRFMQLQQQISAERLQEKVGLTLPVIIDEVDGEGAIGRSMA
DAPEIDGVVYLNGERSVKPGDIVNVTIENADEYDMWGSLA

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=480, Percent_Identity=26.0416666666667, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI28872782, Length=480, Percent_Identity=26.0416666666667, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI93277076, Length=364, Percent_Identity=28.2967032967033, Blast_Score=136, Evalue=3e-32,
Organism=Escherichia coli, GI1787057, Length=439, Percent_Identity=83.5990888382688, Blast_Score=778, Evalue=0.0,
Organism=Escherichia coli, GI1786882, Length=384, Percent_Identity=27.8645833333333, Blast_Score=139, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17553146, Length=472, Percent_Identity=25.2118644067797, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI71996771, Length=259, Percent_Identity=28.1853281853282, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356207, Length=478, Percent_Identity=25.7322175732218, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI19922432, Length=395, Percent_Identity=26.8354430379747, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49261; Mature: 49130

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQSRQPTIGFVSLGCPKNLVDSERILTELRTEGYLVVPTYEDADLVIVNTCGFIDSAV
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCHHHHHH
QESLEAIGEALHENGKVIVTGCLGAKENQIREVHPKVLEISGPHSYEQVLHHVHKYVAKP
HHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCC
QHNPFTSLVPEQGVKLTPKHFAYLKISEGCNHRCAFCIIPSLRGDLESRSIGSVLDEAKR
CCCCCHHCCCCCCCEECCCCEEEEEECCCCCCCEEEEEECCHHCCCHHHHHHHHHHHHHH
LADSGVRELLVISQDTAAYGADIQHRTGFWNGQPVKTSMLSLCEQLATLGVWIRLHYVYP
HHHCCHHEEEEEECCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEEEECC
YPHVDDVIPLMAQGKLLPYLDIPLQHASPRILKLMKRPGAVERTLERIKRWREICPQLTL
CCCHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEE
RSTFIVGFPGETEEDFQMLLDFLREARLDRVGCFQYSPVEGAAANELPDQVPDEIKQARF
CCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHCCCCCCHHHHHHHHH
DRFMQLQQQISAERLQEKVGLTLPVIIDEVDGEGAIGRSMADAPEIDGVVYLNGERSVKP
HHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
GDIVNVTIENADEYDMWGSLA
CCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SNQSRQPTIGFVSLGCPKNLVDSERILTELRTEGYLVVPTYEDADLVIVNTCGFIDSAV
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCHHHHHH
QESLEAIGEALHENGKVIVTGCLGAKENQIREVHPKVLEISGPHSYEQVLHHVHKYVAKP
HHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCC
QHNPFTSLVPEQGVKLTPKHFAYLKISEGCNHRCAFCIIPSLRGDLESRSIGSVLDEAKR
CCCCCHHCCCCCCCEECCCCEEEEEECCCCCCCEEEEEECCHHCCCHHHHHHHHHHHHHH
LADSGVRELLVISQDTAAYGADIQHRTGFWNGQPVKTSMLSLCEQLATLGVWIRLHYVYP
HHHCCHHEEEEEECCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEEEECC
YPHVDDVIPLMAQGKLLPYLDIPLQHASPRILKLMKRPGAVERTLERIKRWREICPQLTL
CCCHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEE
RSTFIVGFPGETEEDFQMLLDFLREARLDRVGCFQYSPVEGAAANELPDQVPDEIKQARF
CCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHCCCCCCHHHHHHHHH
DRFMQLQQQISAERLQEKVGLTLPVIIDEVDGEGAIGRSMADAPEIDGVVYLNGERSVKP
HHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
GDIVNVTIENADEYDMWGSLA
CCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA