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The map label for this gene is ghrB [H]

Identifier: 238920427

GI number: 238920427

Start: 2436603

End: 2437553

Strand: Direct

Name: ghrB [H]

Synonym: NT01EI_2538

Alternate gene names: 238920427

Gene position: 2436603-2437553 (Clockwise)

Preceding gene: 238920425

Following gene: 238920428

Centisome position: 63.91

GC content: 56.89

Gene sequence:

>951_bases
GTGATCTACTATTCCGGGCAGTTATTTAGCTTCGCCACTCTTACCGCCGACAACCGCCATCAGGTACAGGCAGCGCTCGC
CGACGCTATCGGCCTGATCGGCTCCGGTGCCACCATCAATACCGCCCTGTTAGCCCATGCGCCACGACTGCGCGCCATCT
CGACCATCTCTGTTGGCATCGATCAGTTCGACGTCGACGACCTTTCTCAACGCGGCATCGCGCTCATGCATACCCCGGGC
GTTCTGACTGAAACCACCGCCGATACACTGTTTATGTTATTGATGATGACTGCCAGAAGGGCACTGGAACTGGCTGAATG
GGTCAAGGCCGGAGCATGGCAGAACAGCATAGACGCCCGTCATTACGGCATCGATGTGCATCATAAAACCATCGGCATCA
TCGGGATGGGGCGAATCGGCTATGCCTTGGCGCAACGCGCGCATGCCGGCTTTAACATGTCGGTGTGCTATCACAGTAAC
CATGCACATCCCGAAGCAGAGCGTGTATTTTCCGCCCAGCGCTGCTCATTGGAAGCCTTGCTCGCCCGTGCCGATTTCGT
CTGTTTGACACTTCCGCTGACCGCCAAGAGCCAAGGATTGATCAATGCGCAGCGACTTGCCCAGATGAAACCTGGCGCGA
TCCTGATCAACGGTTCCCGGGGGAAAATCGTCGACCAACAGGCCCTAATCCAAGCGCTGCAGTCAGGCCAATTACGCGCT
GCCGGGTTGGATGTTTTTGATCCTGAACCGCTCTTTGCTGACGATCCGCTACTCCATTTAGCCAATGTAATAGCACTGCC
GCACATCGGATCGGCGACGCATGAGACCCGCTATGCGATGGCACGCTGTGCGGTGGAGAATCTGATCGCCGCACTGGCTG
GCAATATCCGGCACAACTGCGCCAATCGCCACTCACTCTGTCAGCTCGCTCACTCTTCACGGGCAGAATAA

Upstream 100 bases:

>100_bases
CTGGGTATTGGTGCGATGATCGGCATTGCTCAGTCCGGCTGGTGATTTGGGATATTCAGCGATTGATCAGATCGCATAAA
CCGGACTGAGTTCCCCCTGA

Downstream 100 bases:

>100_bases
GGTTTCCTTAAGCCTGACCGATTATTCTGTAGTTAAACCCGGCAATAACCACCGCGCATTAACATTGGCGTAGGCGGGCG
CTTCTCCCTATAGTTTAGTG

Product: 2-ketogluconate reductase (2KR)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MIYYSGQLFSFATLTADNRHQVQAALADAIGLIGSGATINTALLAHAPRLRAISTISVGIDQFDVDDLSQRGIALMHTPG
VLTETTADTLFMLLMMTARRALELAEWVKAGAWQNSIDARHYGIDVHHKTIGIIGMGRIGYALAQRAHAGFNMSVCYHSN
HAHPEAERVFSAQRCSLEALLARADFVCLTLPLTAKSQGLINAQRLAQMKPGAILINGSRGKIVDQQALIQALQSGQLRA
AGLDVFDPEPLFADDPLLHLANVIALPHIGSATHETRYAMARCAVENLIAALAGNIRHNCANRHSLCQLAHSSRAE

Sequences:

>Translated_316_residues
MIYYSGQLFSFATLTADNRHQVQAALADAIGLIGSGATINTALLAHAPRLRAISTISVGIDQFDVDDLSQRGIALMHTPG
VLTETTADTLFMLLMMTARRALELAEWVKAGAWQNSIDARHYGIDVHHKTIGIIGMGRIGYALAQRAHAGFNMSVCYHSN
HAHPEAERVFSAQRCSLEALLARADFVCLTLPLTAKSQGLINAQRLAQMKPGAILINGSRGKIVDQQALIQALQSGQLRA
AGLDVFDPEPLFADDPLLHLANVIALPHIGSATHETRYAMARCAVENLIAALAGNIRHNCANRHSLCQLAHSSRAE
>Mature_316_residues
MIYYSGQLFSFATLTADNRHQVQAALADAIGLIGSGATINTALLAHAPRLRAISTISVGIDQFDVDDLSQRGIALMHTPG
VLTETTADTLFMLLMMTARRALELAEWVKAGAWQNSIDARHYGIDVHHKTIGIIGMGRIGYALAQRAHAGFNMSVCYHSN
HAHPEAERVFSAQRCSLEALLARADFVCLTLPLTAKSQGLINAQRLAQMKPGAILINGSRGKIVDQQALIQALQSGQLRA
AGLDVFDPEPLFADDPLLHLANVIALPHIGSATHETRYAMARCAVENLIAALAGNIRHNCANRHSLCQLAHSSRAE

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=246, Percent_Identity=44.3089430894309, Blast_Score=197, Evalue=7e-51,
Organism=Homo sapiens, GI23308577, Length=270, Percent_Identity=34.4444444444444, Blast_Score=151, Evalue=8e-37,
Organism=Homo sapiens, GI61743967, Length=299, Percent_Identity=32.4414715719064, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI4557497, Length=299, Percent_Identity=32.4414715719064, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI145580578, Length=264, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI4557499, Length=264, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI145580575, Length=264, Percent_Identity=33.3333333333333, Blast_Score=128, Evalue=7e-30,
Organism=Escherichia coli, GI87082289, Length=293, Percent_Identity=59.0443686006826, Blast_Score=351, Evalue=4e-98,
Organism=Escherichia coli, GI1789279, Length=295, Percent_Identity=30.8474576271186, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787645, Length=213, Percent_Identity=28.6384976525822, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87081824, Length=169, Percent_Identity=33.7278106508876, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788660, Length=255, Percent_Identity=31.7647058823529, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=267, Percent_Identity=30.7116104868914, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI25147481, Length=274, Percent_Identity=28.1021897810219, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324055, Length=274, Percent_Identity=34.6715328467153, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6320925, Length=268, Percent_Identity=33.2089552238806, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6322116, Length=268, Percent_Identity=32.4626865671642, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324964, Length=257, Percent_Identity=29.1828793774319, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6325144, Length=173, Percent_Identity=31.7919075144509, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6324980, Length=186, Percent_Identity=31.1827956989247, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321253, Length=169, Percent_Identity=30.1775147928994, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28574284, Length=255, Percent_Identity=42.3529411764706, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI45551003, Length=255, Percent_Identity=42.3529411764706, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24585514, Length=255, Percent_Identity=42.3529411764706, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI28574282, Length=255, Percent_Identity=42.3529411764706, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI45552429, Length=255, Percent_Identity=42.3529411764706, Blast_Score=195, Evalue=3e-50,
Organism=Drosophila melanogaster, GI28574286, Length=281, Percent_Identity=39.8576512455516, Blast_Score=189, Evalue=1e-48,
Organism=Drosophila melanogaster, GI28571528, Length=253, Percent_Identity=39.9209486166008, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24585516, Length=258, Percent_Identity=33.7209302325581, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24646446, Length=264, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24646448, Length=264, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24646452, Length=264, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24646450, Length=264, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI62472511, Length=264, Percent_Identity=32.9545454545455, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI19921140, Length=250, Percent_Identity=27.6, Blast_Score=115, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 34078; Mature: 34078

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYYSGQLFSFATLTADNRHQVQAALADAIGLIGSGATINTALLAHAPRLRAISTISVGI
CEEECCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHEEEEEEECC
DQFDVDDLSQRGIALMHTPGVLTETTADTLFMLLMMTARRALELAEWVKAGAWQNSIDAR
CCCCHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
HYGIDVHHKTIGIIGMGRIGYALAQRAHAGFNMSVCYHSNHAHPEAERVFSAQRCSLEAL
CCCEEEECEEEEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
LARADFVCLTLPLTAKSQGLINAQRLAQMKPGAILINGSRGKIVDQQALIQALQSGQLRA
HHHCCEEEEEEECCCCCCCCCCHHHHHHCCCCEEEEECCCCCEECHHHHHHHHHCCCEEE
AGLDVFDPEPLFADDPLLHLANVIALPHIGSATHETRYAMARCAVENLIAALAGNIRHNC
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANRHSLCQLAHSSRAE
CCHHHHHHHHHHCCCC
>Mature Secondary Structure
MIYYSGQLFSFATLTADNRHQVQAALADAIGLIGSGATINTALLAHAPRLRAISTISVGI
CEEECCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHEEEEEEECC
DQFDVDDLSQRGIALMHTPGVLTETTADTLFMLLMMTARRALELAEWVKAGAWQNSIDAR
CCCCHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
HYGIDVHHKTIGIIGMGRIGYALAQRAHAGFNMSVCYHSNHAHPEAERVFSAQRCSLEAL
CCCEEEECEEEEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
LARADFVCLTLPLTAKSQGLINAQRLAQMKPGAILINGSRGKIVDQQALIQALQSGQLRA
HHHCCEEEEEEECCCCCCCCCCHHHHHHCCCCEEEEECCCCCEECHHHHHHHHHCCCEEE
AGLDVFDPEPLFADDPLLHLANVIALPHIGSATHETRYAMARCAVENLIAALAGNIRHNC
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANRHSLCQLAHSSRAE
CCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA