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The map label for this gene is ymfK [C]

Identifier: 238920196

GI number: 238920196

Start: 2201964

End: 2202635

Strand: Direct

Name: ymfK [C]

Synonym: NT01EI_2305

Alternate gene names: 238920196

Gene position: 2201964-2202635 (Clockwise)

Preceding gene: 238920195

Following gene: 238920197

Centisome position: 57.76

GC content: 48.96

Gene sequence:

>672_bases
ATGGATAAATACGAGATAAGAAGGCTTCAGCTGATCAAGCTGAGGGACGAAATGTGTGGAGGGAAAGCTGTTGATTTAGC
AAGAAAAATCAGCAGGGAGCCGTCATACATTTCAAGAATGCTTTATCCTGAAGGGAAAAAGCAGAAGAAGCGAATAGCTG
ATGATATGGTCGAGGTGATCGAGTCTGCTTTTGGCCTTCCCCGCGGCTGGATGGATAGAATTAATGATGGTTATCATGAT
GAAAAATCCATCTCATCAATAAATAAATACCCTCTATTCACTACTGTTCAGGCAGGGGCATTCACCTCTACCCCTGAGTC
ATACGCGGAGAAAGATGCCAAGGCCTGGATAGAGACGTCCAAAAAGGCTGGTTCTCGTTCGTTCTGGCTTGAGGTTGACG
GCGCATCGATGACGGCGCCGGCGGGAAACCGCCCCAGCTTTCCCGAAGGGATGCTGATCTTGGTCGACCCTGATCAGGAT
GTTGAAGTGAACGACTTCTGCATTGCACGCGTGAACGGCAACGAATTCACTTTTAAGAAACTGATCCGCGATAGCGGGAT
CAATTACTTACAGCCGCTCAACCCACAGTTTCCCCTCCTAACGTGTAGTGATGGCTGCACGTTCGTGGGGAAAGTGATCA
TGTCGCAGTGGCCGGAAGAGATGTTTGGCTGA

Upstream 100 bases:

>100_bases
TCTATCTCCCGTTACGATGAAATCAGCTTACCCATAGGTAAATAACCAGTCAATACCCGCAGGTTATTTACCTCTTGGTA
TTTGGAGGTAAACTTTAAAA

Downstream 100 bases:

>100_bases
GTCGCTAGAAGTTATGGGTTACGCCTAGCTCGTAATGGTATCAGACTGGTGTAACGCCATGTATGTATGGACATGGCTCA
TGCATCAATCTGCCACCATT

Product: repressor protein CI

Products: NA

Alternate protein names: ORF-96 protein [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MDKYEIRRLQLIKLRDEMCGGKAVDLARKISREPSYISRMLYPEGKKQKKRIADDMVEVIESAFGLPRGWMDRINDGYHD
EKSISSINKYPLFTTVQAGAFTSTPESYAEKDAKAWIETSKKAGSRSFWLEVDGASMTAPAGNRPSFPEGMLILVDPDQD
VEVNDFCIARVNGNEFTFKKLIRDSGINYLQPLNPQFPLLTCSDGCTFVGKVIMSQWPEEMFG

Sequences:

>Translated_223_residues
MDKYEIRRLQLIKLRDEMCGGKAVDLARKISREPSYISRMLYPEGKKQKKRIADDMVEVIESAFGLPRGWMDRINDGYHD
EKSISSINKYPLFTTVQAGAFTSTPESYAEKDAKAWIETSKKAGSRSFWLEVDGASMTAPAGNRPSFPEGMLILVDPDQD
VEVNDFCIARVNGNEFTFKKLIRDSGINYLQPLNPQFPLLTCSDGCTFVGKVIMSQWPEEMFG
>Mature_223_residues
MDKYEIRRLQLIKLRDEMCGGKAVDLARKISREPSYISRMLYPEGKKQKKRIADDMVEVIESAFGLPRGWMDRINDGYHD
EKSISSINKYPLFTTVQAGAFTSTPESYAEKDAKAWIETSKKAGSRSFWLEVDGASMTAPAGNRPSFPEGMLILVDPDQD
VEVNDFCIARVNGNEFTFKKLIRDSGINYLQPLNPQFPLLTCSDGCTFVGKVIMSQWPEEMFG

Specific function: May control the expression of the integrase and inhibit excision of the mobile element R391, and regulate the expression of other genes as well [H]

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787391, Length=224, Percent_Identity=32.1428571428571, Blast_Score=87, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056 [H]

Pfam domain/function: PF01381 HTH_3; PF00717 Peptidase_S24 [H]

EC number: NA

Molecular weight: Translated: 25227; Mature: 25227

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKYEIRRLQLIKLRDEMCGGKAVDLARKISREPSYISRMLYPEGKKQKKRIADDMVEVI
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
ESAFGLPRGWMDRINDGYHDEKSISSINKYPLFTTVQAGAFTSTPESYAEKDAKAWIETS
HHHHCCCHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
KKAGSRSFWLEVDGASMTAPAGNRPSFPEGMLILVDPDQDVEVNDFCIARVNGNEFTFKK
HCCCCCEEEEEECCCEEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCHHHHH
LIRDSGINYLQPLNPQFPLLTCSDGCTFVGKVIMSQWPEEMFG
HHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCHHHHCC
>Mature Secondary Structure
MDKYEIRRLQLIKLRDEMCGGKAVDLARKISREPSYISRMLYPEGKKQKKRIADDMVEVI
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
ESAFGLPRGWMDRINDGYHDEKSISSINKYPLFTTVQAGAFTSTPESYAEKDAKAWIETS
HHHHCCCHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
KKAGSRSFWLEVDGASMTAPAGNRPSFPEGMLILVDPDQDVEVNDFCIARVNGNEFTFKK
HCCCCCEEEEEECCCEEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCHHHHH
LIRDSGINYLQPLNPQFPLLTCSDGCTFVGKVIMSQWPEEMFG
HHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11157923; 12193633 [H]