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The map label for this gene is insF1 [H]

Identifier: 238920123

GI number: 238920123

Start: 2143785

End: 2144585

Strand: Direct

Name: insF1 [H]

Synonym: NT01EI_2229

Alternate gene names: 238920123

Gene position: 2143785-2144585 (Clockwise)

Preceding gene: 238920122

Following gene: 238920124

Centisome position: 56.23

GC content: 54.06

Gene sequence:

>801_bases
GTGCTCCGTGTCGCCCGTAGTGGCTGGTATGCCTGGCGTCTGCGTCGTCATCAGATAACCCCGCGTCAGCAGTTCCGGCT
CGCTTGTGATGAGGCTGTCCGTAATGCATTCGCTGAGGCAAAACAGCGCTATGGTGCGCCACGCCTTGCTGATGAGTTGC
CGGAGTACAACATTAAAACCATCGCTGCCAGTCTGCGCCGTCAGGGATTACGGGCGAAAGCGAGCCGTAAATTCAGCCCG
GTCAGTTACCATGAACATGGCCTGCCGGTGTCGGAAAACCTGTTGAAGCAGGACGTAACAGCCTGTGGGCCGAATCAGAA
ATGGTCGGGTGACATTATCTACTTACGTACAGACGAAGGTTGGCTTTACCTAGCAGTGGTGATTGACCTGTGGTCGCGGG
CCGTCATCGGCTGGTCGATGTCTTCGCGGATGACGGCACAACTGGCCTGCGATGCGTTGCAAATGGCGTGGTGGAGGCGT
AAACGCCCGGGAAATGCCATTGTTCATACAGACAGAGGCGGCCAGTACTGTTCGGCGGATTATCAGACTTTACTGAAACA
CCATAACCTGCGTGGCAGTATGAGCGCCAAAGGCTGTTGTTACGACAATGCCTGTGCGGAAAGCTTCTTCCACACACTGA
AAGTGGAATGTATCCACGGCAAGCACTTTGCCAGTCGGGAAATGATGCGAACGACTGTGCTTAATTATATCGAGTGCGAT
TACAATCGCTGGCGTCGCCACAATGCCTGTGGTGGTCTTAGCCCGGAACAATTTGAAAAACAGAACCGAGCCTGTACATA
A

Upstream 100 bases:

>100_bases
TTCTCCAAAAGGCCGCGACATACTTTGCGAAGCGCCTGAAATCAAGTATGTTTTTATCGAAAAACATCAGACTGAGTTCA
GCATCAAAGCCATGTGCCGA

Downstream 100 bases:

>100_bases
ATTTGTGTAATTGCCTGATTTTGATATGTTCAATCCAACATCAAAAGCAGGTTACTTTATGAACGAAAAACAGTTGCAGG
CTCTGGCTAACGAACTGGCC

Product: transposase InsF for insertion sequence IS3A/B/C/D/E/fA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MLRVARSGWYAWRLRRHQITPRQQFRLACDEAVRNAFAEAKQRYGAPRLADELPEYNIKTIAASLRRQGLRAKASRKFSP
VSYHEHGLPVSENLLKQDVTACGPNQKWSGDIIYLRTDEGWLYLAVVIDLWSRAVIGWSMSSRMTAQLACDALQMAWWRR
KRPGNAIVHTDRGGQYCSADYQTLLKHHNLRGSMSAKGCCYDNACAESFFHTLKVECIHGKHFASREMMRTTVLNYIECD
YNRWRRHNACGGLSPEQFEKQNRACT

Sequences:

>Translated_266_residues
MLRVARSGWYAWRLRRHQITPRQQFRLACDEAVRNAFAEAKQRYGAPRLADELPEYNIKTIAASLRRQGLRAKASRKFSP
VSYHEHGLPVSENLLKQDVTACGPNQKWSGDIIYLRTDEGWLYLAVVIDLWSRAVIGWSMSSRMTAQLACDALQMAWWRR
KRPGNAIVHTDRGGQYCSADYQTLLKHHNLRGSMSAKGCCYDNACAESFFHTLKVECIHGKHFASREMMRTTVLNYIECD
YNRWRRHNACGGLSPEQFEKQNRACT
>Mature_266_residues
MLRVARSGWYAWRLRRHQITPRQQFRLACDEAVRNAFAEAKQRYGAPRLADELPEYNIKTIAASLRRQGLRAKASRKFSP
VSYHEHGLPVSENLLKQDVTACGPNQKWSGDIIYLRTDEGWLYLAVVIDLWSRAVIGWSMSSRMTAQLACDALQMAWWRR
KRPGNAIVHTDRGGQYCSADYQTLLKHHNLRGSMSAKGCCYDNACAESFFHTLKVECIHGKHFASREMMRTTVLNYIECD
YNRWRRHNACGGLSPEQFEKQNRACT

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=193, Percent_Identity=35.7512953367876, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1788405, Length=268, Percent_Identity=79.8507462686567, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1787263, Length=268, Percent_Identity=79.8507462686567, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1786753, Length=268, Percent_Identity=79.8507462686567, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1786570, Length=268, Percent_Identity=79.8507462686567, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1786490, Length=268, Percent_Identity=79.8507462686567, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1789981, Length=169, Percent_Identity=36.6863905325444, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 30704; Mature: 30704

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRVARSGWYAWRLRRHQITPRQQFRLACDEAVRNAFAEAKQRYGAPRLADELPEYNIKT
CCEECCCCHHEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHH
IAASLRRQGLRAKASRKFSPVSYHEHGLPVSENLLKQDVTACGPNQKWSGDIIYLRTDEG
HHHHHHHHCHHHHHHCCCCCCCHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCC
WLYLAVVIDLWSRAVIGWSMSSRMTAQLACDALQMAWWRRKRPGNAIVHTDRGGQYCSAD
CEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECHH
YQTLLKHHNLRGSMSAKGCCYDNACAESFFHTLKVECIHGKHFASREMMRTTVLNYIECD
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHCC
YNRWRRHNACGGLSPEQFEKQNRACT
HHHHHHCCCCCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MLRVARSGWYAWRLRRHQITPRQQFRLACDEAVRNAFAEAKQRYGAPRLADELPEYNIKT
CCEECCCCHHEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHH
IAASLRRQGLRAKASRKFSPVSYHEHGLPVSENLLKQDVTACGPNQKWSGDIIYLRTDEG
HHHHHHHHCHHHHHHCCCCCCCHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCC
WLYLAVVIDLWSRAVIGWSMSSRMTAQLACDALQMAWWRRKRPGNAIVHTDRGGQYCSAD
CEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECHH
YQTLLKHHNLRGSMSAKGCCYDNACAESFFHTLKVECIHGKHFASREMMRTTVLNYIECD
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHCC
YNRWRRHNACGGLSPEQFEKQNRACT
HHHHHHCCCCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]