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The map label for this gene is amiD [H]

Identifier: 238920114

GI number: 238920114

Start: 2133931

End: 2134815

Strand: Reverse

Name: amiD [H]

Synonym: NT01EI_2220

Alternate gene names: 238920114

Gene position: 2134815-2133931 (Counterclockwise)

Preceding gene: 238920116

Following gene: 238920107

Centisome position: 56.0

GC content: 44.86

Gene sequence:

>885_bases
ATGCGTAAACCTCTGTTTTTACCCTGCTCAACGATGATTGAGAAATTAGTGGTAGTGACCATTCTGGCTTTTATGTTATC
AGCTTGCGTAAGACATTCGGATAAAATTGAAGCGCGCGACGGTTATTTTGCCAGTCATGCTTTTACGGCTGTTGGCCAGA
ATGAAAGGATACGTTTTCTTGTTCTACACTATACGGCAGTGGATGATGTTAGTTCGCTGAATATTTTGACTAAAGAACAA
GTCAGTGCTCATTATCTTATTCCAAAAGAGCCGAAGTCAATACATGGTCAACCCGTCGTCCTTCAGCTCGTTGATGAGAA
TAAGCGGGCATGGCATGCAGGCATCAGCAGCTGGAATGGCCGGTCGGGGCTGAATGATACCTCTATCGGAATAGAAATAG
TCAACCTAGGGTATACTGATAATATGCTGGGGCAACGAACCTGGTATCCCTACACCGAAAAACAGATTGCTGCTGTCGCT
GCATTAGCAAAGGATATTGTTAATCGCTATCATATTGAGCCTGATAATGTGGTTGGACACAGTGACATAGCCCCTCTCAG
AAAACAGGACCCCGGAAAACTATTCCCTTGGGAACACCTGGCTGCAATGGGCATAGGTGCTTGGCCGGACAGAAGTACAG
TAAATAAATATCTTGCTGATCGTTCCCCACATTCCCCTGTAAAAGTAAGTGTTATTCAGGCATTACTGAAGCAGTATGGA
TATGATCAAATCCCCCAGAATGGAGTCTTGGATGAGAATACCAGAAAAACGGTCAGTGCATTCCAGATGCACTTCCGGCC
AAGTGATATCAGCGGTAATGCCGATGCCGAGACTGAAGCCATTGCCCGAGCTTTGCTCGAAAAATATAGAGGAATTTCGT
TTTAG

Upstream 100 bases:

>100_bases
TATCAATCGTTTAATGAAATAGGTGATAATGGCTGCTGTTTTCAATAACTGATTCAAGCAAAGAAGCGGTGGCTTCAATT
CATTAACGGAGTTTAAATTA

Downstream 100 bases:

>100_bases
ACTCGGCCAACAGGGAAATCTTGATGCTGGCAGAGATCAAATTGATTTGATGACAAGGCGCAGTAAACTGACAGAGTTAG
TCATGGGGCTTATTTAAAAA

Product: N-acetylmuramoyl-L-alanine amidase AmiD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MRKPLFLPCSTMIEKLVVVTILAFMLSACVRHSDKIEARDGYFASHAFTAVGQNERIRFLVLHYTAVDDVSSLNILTKEQ
VSAHYLIPKEPKSIHGQPVVLQLVDENKRAWHAGISSWNGRSGLNDTSIGIEIVNLGYTDNMLGQRTWYPYTEKQIAAVA
ALAKDIVNRYHIEPDNVVGHSDIAPLRKQDPGKLFPWEHLAAMGIGAWPDRSTVNKYLADRSPHSPVKVSVIQALLKQYG
YDQIPQNGVLDENTRKTVSAFQMHFRPSDISGNADAETEAIARALLEKYRGISF

Sequences:

>Translated_294_residues
MRKPLFLPCSTMIEKLVVVTILAFMLSACVRHSDKIEARDGYFASHAFTAVGQNERIRFLVLHYTAVDDVSSLNILTKEQ
VSAHYLIPKEPKSIHGQPVVLQLVDENKRAWHAGISSWNGRSGLNDTSIGIEIVNLGYTDNMLGQRTWYPYTEKQIAAVA
ALAKDIVNRYHIEPDNVVGHSDIAPLRKQDPGKLFPWEHLAAMGIGAWPDRSTVNKYLADRSPHSPVKVSVIQALLKQYG
YDQIPQNGVLDENTRKTVSAFQMHFRPSDISGNADAETEAIARALLEKYRGISF
>Mature_294_residues
MRKPLFLPCSTMIEKLVVVTILAFMLSACVRHSDKIEARDGYFASHAFTAVGQNERIRFLVLHYTAVDDVSSLNILTKEQ
VSAHYLIPKEPKSIHGQPVVLQLVDENKRAWHAGISSWNGRSGLNDTSIGIEIVNLGYTDNMLGQRTWYPYTEKQIAAVA
ALAKDIVNRYHIEPDNVVGHSDIAPLRKQDPGKLFPWEHLAAMGIGAWPDRSTVNKYLADRSPHSPVKVSVIQALLKQYG
YDQIPQNGVLDENTRKTVSAFQMHFRPSDISGNADAETEAIARALLEKYRGISF

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=262, Percent_Identity=51.5267175572519, Blast_Score=261, Evalue=3e-71,
Organism=Escherichia coli, GI1786300, Length=124, Percent_Identity=37.9032258064516, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 32880; Mature: 32880

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPLFLPCSTMIEKLVVVTILAFMLSACVRHSDKIEARDGYFASHAFTAVGQNERIRFL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHCCCCCCCEEEE
VLHYTAVDDVSSLNILTKEQVSAHYLIPKEPKSIHGQPVVLQLVDENKRAWHAGISSWNG
EEEEEHHHCHHHHHHEEHHHCCEEEECCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCCC
RSGLNDTSIGIEIVNLGYTDNMLGQRTWYPYTEKQIAAVAALAKDIVNRYHIEPDNVVGH
CCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
SDIAPLRKQDPGKLFPWEHLAAMGIGAWPDRSTVNKYLADRSPHSPVKVSVIQALLKQYG
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
YDQIPQNGVLDENTRKTVSAFQMHFRPSDISGNADAETEAIARALLEKYRGISF
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRKPLFLPCSTMIEKLVVVTILAFMLSACVRHSDKIEARDGYFASHAFTAVGQNERIRFL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHCCCCCCCEEEE
VLHYTAVDDVSSLNILTKEQVSAHYLIPKEPKSIHGQPVVLQLVDENKRAWHAGISSWNG
EEEEEHHHCHHHHHHEEHHHCCEEEECCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCCC
RSGLNDTSIGIEIVNLGYTDNMLGQRTWYPYTEKQIAAVAALAKDIVNRYHIEPDNVVGH
CCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
SDIAPLRKQDPGKLFPWEHLAAMGIGAWPDRSTVNKYLADRSPHSPVKVSVIQALLKQYG
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
YDQIPQNGVLDENTRKTVSAFQMHFRPSDISGNADAETEAIARALLEKYRGISF
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]