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The map label for this gene is rbsB [H]

Identifier: 238920030

GI number: 238920030

Start: 2062352

End: 2063281

Strand: Reverse

Name: rbsB [H]

Synonym: NT01EI_2134

Alternate gene names: 238920030

Gene position: 2063281-2062352 (Counterclockwise)

Preceding gene: 238920031

Following gene: 238920029

Centisome position: 54.12

GC content: 61.18

Gene sequence:

>930_bases
ATGAAAAAACTGATCTTTGCCGCCCTTATCACCGTGATATCCGGAGCGGTTCAGGCCGAGAACGAGCAGATTGTTTTCAG
TACGCCAAACCTGGCGATGCCGTTTGAGGTTCACATGCAGCGCACGGCGGTCAAAGCCGCCAGGGAGATGGGGGTGAAGC
TGCAGGTACTGGACGGGCTGGGTAGTTCGCCAAAGCAGGTGGCTGACCTTGAAAACGCCATCACCCGTGGTGCACAGGGG
TTTATCGTGTCACCCAATGACGTTAACGCCGTTTCCGGCGCGGTTGATGAGATCCAGGCCGCCAGGCTGCCGGTGGTGAC
CCTCGACCGCTCCGTCGACAGTCAGAATAAGGTGCCGCACTTCGGCGCAAACAACTACAAAGGTGGCCAGGCCATTGGCG
ACTTTGTGAAGAGCCAGTTCCCCAACGGCGCGGACATTATTCTGCTGACCGGGCAGCCGGGCTCGTCGTCTAACATCGAG
CGTAGCAAGGGGATCCGCGACAGCCTGAAGGCGGGGGGGGATAAGTACAAAATCGTCACCGATCAGACAGGTAACTGGAT
GCGCTCTGAAGGGATGCGCATTGTCGAGAGCGTCCTGCCATCGCTGGCCCGGCGTCCACAGGTGATCCTCTCTGCCAACG
ATGATATGGCGCTGGGCGCGATTGAGGCGCTGCAGAGCCAGGGACTCAAGCCGGGCGATGTGCTGGTGACGGGCTTTGAC
GCCGTGCCGGAAGCCCTGGCGCGGGTGCGCGACGGCTGGATGGCGGTGACGGCCGATCAGCGTCCGGGCTATGCGGTGCA
GACAGCCATGAGCCAGCTGGTGGCCAGCGTGCGTGAGAAGCAGGCGATCGTTGGCGCAGACTATCCGCCGACGCTTATCA
CCCGTGAAAACCTGCAGCAGGCCGAGCGGATTAACGAGGCCGGTAACTGA

Upstream 100 bases:

>100_bases
CGCTGTATCTGGCGGATAAAGCGTTGGAGTCGCTGCTCTGCCAGCGAGAGGTCTCCCTCTAAGAAGCGTAATAACTTTCC
CTACACAGAGAGACATCACA

Downstream 100 bases:

>100_bases
TTTGTCCTGGGCTGCCACGGCGGTGGCCCACTATGAAGAAGGAGCAGGCCGATGTCGCAACCTTTACTCAATGTCACCCA
TCTGGCGAAAAGCTTCTCTG

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKKLIFAALITVISGAVQAENEQIVFSTPNLAMPFEVHMQRTAVKAAREMGVKLQVLDGLGSSPKQVADLENAITRGAQG
FIVSPNDVNAVSGAVDEIQAARLPVVTLDRSVDSQNKVPHFGANNYKGGQAIGDFVKSQFPNGADIILLTGQPGSSSNIE
RSKGIRDSLKAGGDKYKIVTDQTGNWMRSEGMRIVESVLPSLARRPQVILSANDDMALGAIEALQSQGLKPGDVLVTGFD
AVPEALARVRDGWMAVTADQRPGYAVQTAMSQLVASVREKQAIVGADYPPTLITRENLQQAERINEAGN

Sequences:

>Translated_309_residues
MKKLIFAALITVISGAVQAENEQIVFSTPNLAMPFEVHMQRTAVKAAREMGVKLQVLDGLGSSPKQVADLENAITRGAQG
FIVSPNDVNAVSGAVDEIQAARLPVVTLDRSVDSQNKVPHFGANNYKGGQAIGDFVKSQFPNGADIILLTGQPGSSSNIE
RSKGIRDSLKAGGDKYKIVTDQTGNWMRSEGMRIVESVLPSLARRPQVILSANDDMALGAIEALQSQGLKPGDVLVTGFD
AVPEALARVRDGWMAVTADQRPGYAVQTAMSQLVASVREKQAIVGADYPPTLITRENLQQAERINEAGN
>Mature_309_residues
MKKLIFAALITVISGAVQAENEQIVFSTPNLAMPFEVHMQRTAVKAAREMGVKLQVLDGLGSSPKQVADLENAITRGAQG
FIVSPNDVNAVSGAVDEIQAARLPVVTLDRSVDSQNKVPHFGANNYKGGQAIGDFVKSQFPNGADIILLTGQPGSSSNIE
RSKGIRDSLKAGGDKYKIVTDQTGNWMRSEGMRIVESVLPSLARRPQVILSANDDMALGAIEALQSQGLKPGDVLVTGFD
AVPEALARVRDGWMAVTADQRPGYAVQTAMSQLVASVREKQAIVGADYPPTLITRENLQQAERINEAGN

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=267, Percent_Identity=32.5842696629214, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1790674, Length=227, Percent_Identity=29.0748898678414, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1790526, Length=283, Percent_Identity=26.5017667844523, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789990, Length=217, Percent_Identity=27.6497695852535, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788473, Length=223, Percent_Identity=25.5605381165919, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789068, Length=201, Percent_Identity=26.3681592039801, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32962; Mature: 32962

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLIFAALITVISGAVQAENEQIVFSTPNLAMPFEVHMQRTAVKAAREMGVKLQVLDGL
CHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCC
GSSPKQVADLENAITRGAQGFIVSPNDVNAVSGAVDEIQAARLPVVTLDRSVDSQNKVPH
CCCHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
FGANNYKGGQAIGDFVKSQFPNGADIILLTGQPGSSSNIERSKGIRDSLKAGGDKYKIVT
CCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHCCCHHHHHCCCCEEEEEE
DQTGNWMRSEGMRIVESVLPSLARRPQVILSANDDMALGAIEALQSQGLKPGDVLVTGFD
CCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEEECHH
AVPEALARVRDGWMAVTADQRPGYAVQTAMSQLVASVREKQAIVGADYPPTLITRENLQQ
HHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHH
AERINEAGN
HHHHHCCCC
>Mature Secondary Structure
MKKLIFAALITVISGAVQAENEQIVFSTPNLAMPFEVHMQRTAVKAAREMGVKLQVLDGL
CHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCC
GSSPKQVADLENAITRGAQGFIVSPNDVNAVSGAVDEIQAARLPVVTLDRSVDSQNKVPH
CCCHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
FGANNYKGGQAIGDFVKSQFPNGADIILLTGQPGSSSNIERSKGIRDSLKAGGDKYKIVT
CCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHCCCHHHHHCCCCEEEEEE
DQTGNWMRSEGMRIVESVLPSLARRPQVILSANDDMALGAIEALQSQGLKPGDVLVTGFD
CCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEEECHH
AVPEALARVRDGWMAVTADQRPGYAVQTAMSQLVASVREKQAIVGADYPPTLITRENLQQ
HHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHH
AERINEAGN
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]