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The map label for this gene is cusS [H]

Identifier: 238920017

GI number: 238920017

Start: 2042995

End: 2044470

Strand: Direct

Name: cusS [H]

Synonym: NT01EI_2121

Alternate gene names: 238920017

Gene position: 2042995-2044470 (Clockwise)

Preceding gene: 238920016

Following gene: 238920020

Centisome position: 53.59

GC content: 61.86

Gene sequence:

>1476_bases
GTGCGACGCCCTATCTCCCTGGCAGCCCGCCTGACCCTGACCATCTCGCTGGCGACCAGCCTCGCCTTCGCCCTGTTCAC
CTGGATCATGATCTACTCCGTCGAGCGCCATTTTGCCGAGCTGGACAACGGCACGCTCCAGCAGGTGCGCAGCAGCATCC
TGCGCGTCTTGCAAGAGCCGCCCCGGGCCGGCGTCCAGCGCCTGGAAACGCTGAACCGCGTGCTGGCCGGTTATCAGAAC
ATCAGCGTCGCCCTACGCGCCAGCGACCAGCGTTTTATCTACCGATCGGACTCCGCCACCACCCTCAATGCCGTCTGGCA
GCTACCGGAGTTGCCACACTATCTGCAAAGCGGTCAAGTTTTCCTGTGGCCGAATGAGCGTCACGCCACCGCCAACCCGC
GTGAGGCAACGCCCCCCGAGGCCTATCGCCTCATCGCCGCCACGGTCTATCCGGCGGGTGACCCAGGCGCGCCCTACACC
CTGCTGATCGCCCTCTCTATCAACGTACACCTGCACTATCTTGAGCAGCTGAAGAGGGATCTGATGATCTGCGCGCTCTT
TATCAGCCTGCTGATCATTTTCATCGTGCTGCTCGCCGTCTACAGCGGACACCGGCCACTGCGCGATCTCAGCGGGAAGA
TCAAGGCCATCACCGCGGAGAATCTCGATACCCGTCTCGCCCCCGACACGGTGCCGATCGAGCTGCAACAGCTGGTGCTC
TCCTTCAACCAGATGCTCGCCCGCATCGAAGATGTCTTCACCCGGCAGGCAAACTTTTCTGCCGACATCGCCCACGAGAT
GCGTACCCCGATCACCAATCTGATCACCCAAAGCGAAATCGTGCTGAGCCAGGCACGTACGGAGAACGAACTGAAGGAGG
TAATCTATTCGAACCTCGAAGAGTACCAGCGTATGGCGAAGATGGTGAACGACATGCTATTTCTGGCGCAGGCGGACAGT
CAGCGCCTTCACCTCAGCTGCGTTCCCCTCGATCTGCACCGGGAGATCCTCACGGTATTTGACTTCTTCGAGGCCTGGGC
GGAGGAGCGCCAGGTAACCCTGCGCCTTGACGGCAAGGGCCACGCCGTCTGGGGTGACCCGCCGATGCTGCGGCGGGTCA
TCAGCAATCTGCTCTCCAACGCCATTCGCCATACCCCCGCAGGCCACAGCGTGATCGTACGGCTCAGCGAGACGGCCGAC
AACGTGCTGCTCTGCGTCGCCAATCCCGGCATGCCCATCGCTGCGCAGCACCTGCCGCGCCTGTTCGACCGTTTCTATCG
CGTCGACCCCGCACGCCAACGTAATGGTGAGGGAAGCGGCATCGGCCTGGCCATTGTCAAATCGATCATCGACGCGCACC
GGGGCCAGATCTGGGTTAGCTCGGATCCCGACGCCACCCGCTTTACCTTCTCCCTGCCGCGCGCTGCGCTGGACGAGGCA
CGGGATATCACATCCCATGACAGAATAACATTTTAA

Upstream 100 bases:

>100_bases
CGACTGCGGGCGAAGATCGACCTCGACTTCGAGCCTAAGCTGATCCAGACGGTGCGCGGCGTCGGCTATCTGCTTGAGGC
GCCGGATGAGTGTTAAGCGC

Downstream 100 bases:

>100_bases
TAATCAATGCAATGATTCATTGTACAACCGTTATTCTCTCATTAGATATGACGGTGGATCGTCCCGGTATACCGCGGCGA
TACCTCCGCTGAACCGCAGC

Product: sensor kinase CusS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MRRPISLAARLTLTISLATSLAFALFTWIMIYSVERHFAELDNGTLQQVRSSILRVLQEPPRAGVQRLETLNRVLAGYQN
ISVALRASDQRFIYRSDSATTLNAVWQLPELPHYLQSGQVFLWPNERHATANPREATPPEAYRLIAATVYPAGDPGAPYT
LLIALSINVHLHYLEQLKRDLMICALFISLLIIFIVLLAVYSGHRPLRDLSGKIKAITAENLDTRLAPDTVPIELQQLVL
SFNQMLARIEDVFTRQANFSADIAHEMRTPITNLITQSEIVLSQARTENELKEVIYSNLEEYQRMAKMVNDMLFLAQADS
QRLHLSCVPLDLHREILTVFDFFEAWAEERQVTLRLDGKGHAVWGDPPMLRRVISNLLSNAIRHTPAGHSVIVRLSETAD
NVLLCVANPGMPIAAQHLPRLFDRFYRVDPARQRNGEGSGIGLAIVKSIIDAHRGQIWVSSDPDATRFTFSLPRAALDEA
RDITSHDRITF

Sequences:

>Translated_491_residues
MRRPISLAARLTLTISLATSLAFALFTWIMIYSVERHFAELDNGTLQQVRSSILRVLQEPPRAGVQRLETLNRVLAGYQN
ISVALRASDQRFIYRSDSATTLNAVWQLPELPHYLQSGQVFLWPNERHATANPREATPPEAYRLIAATVYPAGDPGAPYT
LLIALSINVHLHYLEQLKRDLMICALFISLLIIFIVLLAVYSGHRPLRDLSGKIKAITAENLDTRLAPDTVPIELQQLVL
SFNQMLARIEDVFTRQANFSADIAHEMRTPITNLITQSEIVLSQARTENELKEVIYSNLEEYQRMAKMVNDMLFLAQADS
QRLHLSCVPLDLHREILTVFDFFEAWAEERQVTLRLDGKGHAVWGDPPMLRRVISNLLSNAIRHTPAGHSVIVRLSETAD
NVLLCVANPGMPIAAQHLPRLFDRFYRVDPARQRNGEGSGIGLAIVKSIIDAHRGQIWVSSDPDATRFTFSLPRAALDEA
RDITSHDRITF
>Mature_491_residues
MRRPISLAARLTLTISLATSLAFALFTWIMIYSVERHFAELDNGTLQQVRSSILRVLQEPPRAGVQRLETLNRVLAGYQN
ISVALRASDQRFIYRSDSATTLNAVWQLPELPHYLQSGQVFLWPNERHATANPREATPPEAYRLIAATVYPAGDPGAPYT
LLIALSINVHLHYLEQLKRDLMICALFISLLIIFIVLLAVYSGHRPLRDLSGKIKAITAENLDTRLAPDTVPIELQQLVL
SFNQMLARIEDVFTRQANFSADIAHEMRTPITNLITQSEIVLSQARTENELKEVIYSNLEEYQRMAKMVNDMLFLAQADS
QRLHLSCVPLDLHREILTVFDFFEAWAEERQVTLRLDGKGHAVWGDPPMLRRVISNLLSNAIRHTPAGHSVIVRLSETAD
NVLLCVANPGMPIAAQHLPRLFDRFYRVDPARQRNGEGSGIGLAIVKSIIDAHRGQIWVSSDPDATRFTFSLPRAALDEA
RDITSHDRITF

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=479, Percent_Identity=53.4446764091858, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI1788279, Length=305, Percent_Identity=30.1639344262295, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI1786600, Length=279, Percent_Identity=30.8243727598566, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788393, Length=372, Percent_Identity=26.8817204301075, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790346, Length=261, Percent_Identity=28.735632183908, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1790551, Length=289, Percent_Identity=29.0657439446367, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787894, Length=310, Percent_Identity=27.741935483871, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1789403, Length=271, Percent_Identity=32.1033210332103, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI145693157, Length=250, Percent_Identity=28.4, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1789149, Length=243, Percent_Identity=25.9259259259259, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI87081816, Length=280, Percent_Identity=25.7142857142857, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=26.8181818181818, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788713, Length=226, Percent_Identity=25.2212389380531, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI48994928, Length=248, Percent_Identity=25.8064516129032, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1786912, Length=246, Percent_Identity=26.8292682926829, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1787374, Length=326, Percent_Identity=23.6196319018405, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789808, Length=281, Percent_Identity=26.3345195729537, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI87082128, Length=252, Percent_Identity=25, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1790861, Length=231, Percent_Identity=24.2424242424242, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55389; Mature: 55389

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRPISLAARLTLTISLATSLAFALFTWIMIYSVERHFAELDNGTLQQVRSSILRVLQEP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
PRAGVQRLETLNRVLAGYQNISVALRASDQRFIYRSDSATTLNAVWQLPELPHYLQSGQV
CHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEECCCCCCHHHHHCCCCCHHHHHCCCE
FLWPNERHATANPREATPPEAYRLIAATVYPAGDPGAPYTLLIALSINVHLHYLEQLKRD
EEECCCCCCCCCCCCCCCHHHHHEEHEEEECCCCCCCCEEEEEEEEEHHHHHHHHHHHHH
LMICALFISLLIIFIVLLAVYSGHRPLRDLSGKIKAITAENLDTRLAPDTVPIELQQLVL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
SFNQMLARIEDVFTRQANFSADIAHEMRTPITNLITQSEIVLSQARTENELKEVIYSNLE
HHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYQRMAKMVNDMLFLAQADSQRLHLSCVPLDLHREILTVFDFFEAWAEERQVTLRLDGKG
HHHHHHHHHHHHHHHEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
HAVWGDPPMLRRVISNLLSNAIRHTPAGHSVIVRLSETADNVLLCVANPGMPIAAQHLPR
CEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCHHHHHHHH
LFDRFYRVDPARQRNGEGSGIGLAIVKSIIDAHRGQIWVSSDPDATRFTFSLPRAALDEA
HHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCHHHHHHH
RDITSHDRITF
HCCCCCCCCCC
>Mature Secondary Structure
MRRPISLAARLTLTISLATSLAFALFTWIMIYSVERHFAELDNGTLQQVRSSILRVLQEP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
PRAGVQRLETLNRVLAGYQNISVALRASDQRFIYRSDSATTLNAVWQLPELPHYLQSGQV
CHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEECCCCCCHHHHHCCCCCHHHHHCCCE
FLWPNERHATANPREATPPEAYRLIAATVYPAGDPGAPYTLLIALSINVHLHYLEQLKRD
EEECCCCCCCCCCCCCCCHHHHHEEHEEEECCCCCCCCEEEEEEEEEHHHHHHHHHHHHH
LMICALFISLLIIFIVLLAVYSGHRPLRDLSGKIKAITAENLDTRLAPDTVPIELQQLVL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
SFNQMLARIEDVFTRQANFSADIAHEMRTPITNLITQSEIVLSQARTENELKEVIYSNLE
HHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYQRMAKMVNDMLFLAQADSQRLHLSCVPLDLHREILTVFDFFEAWAEERQVTLRLDGKG
HHHHHHHHHHHHHHHEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
HAVWGDPPMLRRVISNLLSNAIRHTPAGHSVIVRLSETADNVLLCVANPGMPIAAQHLPR
CEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCHHHHHHHH
LFDRFYRVDPARQRNGEGSGIGLAIVKSIIDAHRGQIWVSSDPDATRFTFSLPRAALDEA
HHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCHHHHHHH
RDITSHDRITF
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]