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The map label for this gene is fumC [H]

Identifier: 238919974

GI number: 238919974

Start: 1997470

End: 1998876

Strand: Reverse

Name: fumC [H]

Synonym: NT01EI_2078

Alternate gene names: 238919974

Gene position: 1998876-1997470 (Counterclockwise)

Preceding gene: 238919978

Following gene: 238919972

Centisome position: 52.43

GC content: 60.98

Gene sequence:

>1407_bases
ATGACGGCGATGCGTATAGAGAGAGATGCGATGGGGCAGGTGAAGGTGCCTCAGGAGCGTATGTGGGGCGCCCAAACACA
GCGCTCTCTTGAGCATTTCTGTATCTCGGTGGAGAAGATGCCGCCAGCGCTGATTGCAGCGCTGGCGCTGGTTAAGCTGT
GCGCCGCACGGGTGAATCGAGACTATGGGCACCTGACGCCGGAGAAGGCAAACGCCATTATTTTGGCGGCGCAGGAGGTT
CTCGACGGGCAGTGCCCCGGAGAGTTTCCGCTGTCGGTCTGGCAAACGGGTTCCGGCACGCAGACCAATATGAATATGAA
CGAAGTGCTGGCGAACCGGGCCAGTGAGCTGCTGGGTGGCGTCCGCGGCGAGCAGCGGCTGGTGCATCCCAACGATGACG
TCAATTGCGCTCAAAGCTCCAACGATGTATTTCCAACGGCGATGCATGTGGCGGTGGTGCTGGGTATTGAGCGCGATCTG
CTGCCGCGTATCGCCCAGTTGAGCGATACGCTACGGCATAAGGCGCAACGCTTTGCCTCCATTGTGAAGATTGGACGCAC
CCATCTGCAGGATGCCACGCCGCTGACGCTGGGACAGGAGATCTCCGGCTGGGTGGCGATGCTGGAGCACAATGAGCGGC
ATATCAGACACACGCTGCCGCACCTGTATGAGCTGGCACTGGGGGGGACAGCGGTTGGAACCGGGTTGAATACGCCGCCG
GGATTTGCCCAGCGCATTGCCGCCGAGCTTGCCGGACAGATTGGTCTGCCGTTTGTCAGCGCCCCCAATAAGTTTGAGGC
GCTGGCGACCTGTGATGCCCTGGTGCAAGGGCACGGTGCCCTGAAGGGGCTGGCCTGCTCTTTGATGAAGATTGCCAACG
ACGTGCGCTGGCTGGCATCGGGGCCCCGCTGTGGGATAGGGGAGCTGAGACTGCCGGAGAACGAGCCGGGCAGTTCCATC
ATGCCAGGTAAGGTCAACCCGACACAGTGTGAGGCGCTGACCATGGTGTGCGCGCAGATACTGGGGAATGATACCGCCGT
AAATATCGGTGGCGCCTCCGGTAACTTTGAGCTGAACGTCTTTCGCCCGCTGGTGATCCACAATATCTTACAGTCCCTGC
GGCTGTTGGCCGATGGCATGGAGAGCTTTAACCGGCACTGTGCGCAGGGACTGATGCCGAACCGGGAGCGTATCGACGAA
CTGTTGCATCGTTCGCTGATGCTGGTGACGGCGCTGGCCCCGCATATTGGCTACGATCGGGCGGCAGAGATCGCCAAGAA
GGCGCACCGGGAGGGGACGACGCTGCGGGAGGCGGCGCTGACGCTCGGCTATGTCAGCGCACCGGCGTTTGATGAATGGG
TTCAACCTGCACAGATGGTGGGTGATTCCGCGCAAGGTGAAAAGTGA

Upstream 100 bases:

>100_bases
GCATTCACAATGAGGCCATTGACAAGGCAGTATACTGAAAAAGAAGCGGTTAGTTTTTCACCGTAAAGGTGGCGGTCCAG
TGGAGACACGAGGAGAGATA

Downstream 100 bases:

>100_bases
TCATATTGTGACAACGTCACAATTAGTTTTCTAACTCATCGGCCTCACGGTAAAGGTGCAGCCGGGGGATCACCGGGATC
AGCTTCTTGGCCCGTGGGCG

Product: fumarate hydratase, class II

Products: NA

Alternate protein names: Fumarase C [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTAMRIERDAMGQVKVPQERMWGAQTQRSLEHFCISVEKMPPALIAALALVKLCAARVNRDYGHLTPEKANAIILAAQEV
LDGQCPGEFPLSVWQTGSGTQTNMNMNEVLANRASELLGGVRGEQRLVHPNDDVNCAQSSNDVFPTAMHVAVVLGIERDL
LPRIAQLSDTLRHKAQRFASIVKIGRTHLQDATPLTLGQEISGWVAMLEHNERHIRHTLPHLYELALGGTAVGTGLNTPP
GFAQRIAAELAGQIGLPFVSAPNKFEALATCDALVQGHGALKGLACSLMKIANDVRWLASGPRCGIGELRLPENEPGSSI
MPGKVNPTQCEALTMVCAQILGNDTAVNIGGASGNFELNVFRPLVIHNILQSLRLLADGMESFNRHCAQGLMPNRERIDE
LLHRSLMLVTALAPHIGYDRAAEIAKKAHREGTTLREAALTLGYVSAPAFDEWVQPAQMVGDSAQGEK

Sequences:

>Translated_468_residues
MTAMRIERDAMGQVKVPQERMWGAQTQRSLEHFCISVEKMPPALIAALALVKLCAARVNRDYGHLTPEKANAIILAAQEV
LDGQCPGEFPLSVWQTGSGTQTNMNMNEVLANRASELLGGVRGEQRLVHPNDDVNCAQSSNDVFPTAMHVAVVLGIERDL
LPRIAQLSDTLRHKAQRFASIVKIGRTHLQDATPLTLGQEISGWVAMLEHNERHIRHTLPHLYELALGGTAVGTGLNTPP
GFAQRIAAELAGQIGLPFVSAPNKFEALATCDALVQGHGALKGLACSLMKIANDVRWLASGPRCGIGELRLPENEPGSSI
MPGKVNPTQCEALTMVCAQILGNDTAVNIGGASGNFELNVFRPLVIHNILQSLRLLADGMESFNRHCAQGLMPNRERIDE
LLHRSLMLVTALAPHIGYDRAAEIAKKAHREGTTLREAALTLGYVSAPAFDEWVQPAQMVGDSAQGEK
>Mature_467_residues
TAMRIERDAMGQVKVPQERMWGAQTQRSLEHFCISVEKMPPALIAALALVKLCAARVNRDYGHLTPEKANAIILAAQEVL
DGQCPGEFPLSVWQTGSGTQTNMNMNEVLANRASELLGGVRGEQRLVHPNDDVNCAQSSNDVFPTAMHVAVVLGIERDLL
PRIAQLSDTLRHKAQRFASIVKIGRTHLQDATPLTLGQEISGWVAMLEHNERHIRHTLPHLYELALGGTAVGTGLNTPPG
FAQRIAAELAGQIGLPFVSAPNKFEALATCDALVQGHGALKGLACSLMKIANDVRWLASGPRCGIGELRLPENEPGSSIM
PGKVNPTQCEALTMVCAQILGNDTAVNIGGASGNFELNVFRPLVIHNILQSLRLLADGMESFNRHCAQGLMPNRERIDEL
LHRSLMLVTALAPHIGYDRAAEIAKKAHREGTTLREAALTLGYVSAPAFDEWVQPAQMVGDSAQGEK

Specific function: Tricarboxylic acid cycle [C]

COG id: COG0114

COG function: function code C; Fumarase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Fumarase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=461, Percent_Identity=59.4360086767896, Blast_Score=556, Evalue=1e-158,
Organism=Homo sapiens, GI266458395, Length=63, Percent_Identity=53.968253968254, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1787896, Length=466, Percent_Identity=73.3905579399142, Blast_Score=703, Evalue=0.0,
Organism=Escherichia coli, GI87082375, Length=452, Percent_Identity=39.8230088495575, Blast_Score=331, Evalue=7e-92,
Organism=Caenorhabditis elegans, GI17553882, Length=463, Percent_Identity=57.2354211663067, Blast_Score=550, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI32565146, Length=325, Percent_Identity=60.3076923076923, Blast_Score=421, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6324993, Length=462, Percent_Identity=55.8441558441558, Blast_Score=527, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24640179, Length=461, Percent_Identity=57.0498915401301, Blast_Score=555, Evalue=1e-158,
Organism=Drosophila melanogaster, GI24640177, Length=461, Percent_Identity=57.0498915401301, Blast_Score=554, Evalue=1e-158,
Organism=Drosophila melanogaster, GI78710009, Length=462, Percent_Identity=56.2770562770563, Blast_Score=541, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24662684, Length=463, Percent_Identity=56.3714902807775, Blast_Score=530, Evalue=1e-151,
Organism=Drosophila melanogaster, GI24583245, Length=461, Percent_Identity=50.1084598698482, Blast_Score=463, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 50618; Mature: 50487

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAMRIERDAMGQVKVPQERMWGAQTQRSLEHFCISVEKMPPALIAALALVKLCAARVNR
CCCCCCCHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
DYGHLTPEKANAIILAAQEVLDGQCPGEFPLSVWQTGSGTQTNMNMNEVLANRASELLGG
CCCCCCCCCCCEEEEEEHHHHCCCCCCCCCHHHEECCCCCCCCCCHHHHHHHHHHHHHCC
VRGEQRLVHPNDDVNCAQSSNDVFPTAMHVAVVLGIERDLLPRIAQLSDTLRHKAQRFAS
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
IVKIGRTHLQDATPLTLGQEISGWVAMLEHNERHIRHTLPHLYELALGGTAVGTGLNTPP
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GFAQRIAAELAGQIGLPFVSAPNKFEALATCDALVQGHGALKGLACSLMKIANDVRWLAS
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
GPRCGIGELRLPENEPGSSIMPGKVNPTQCEALTMVCAQILGNDTAVNIGGASGNFELNV
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEH
FRPLVIHNILQSLRLLADGMESFNRHCAQGLMPNRERIDELLHRSLMLVTALAPHIGYDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHH
AAEIAKKAHREGTTLREAALTLGYVSAPAFDEWVQPAQMVGDSAQGEK
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHCCHHHHHCCCCCCCC
>Mature Secondary Structure 
TAMRIERDAMGQVKVPQERMWGAQTQRSLEHFCISVEKMPPALIAALALVKLCAARVNR
CCCCCCHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
DYGHLTPEKANAIILAAQEVLDGQCPGEFPLSVWQTGSGTQTNMNMNEVLANRASELLGG
CCCCCCCCCCCEEEEEEHHHHCCCCCCCCCHHHEECCCCCCCCCCHHHHHHHHHHHHHCC
VRGEQRLVHPNDDVNCAQSSNDVFPTAMHVAVVLGIERDLLPRIAQLSDTLRHKAQRFAS
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
IVKIGRTHLQDATPLTLGQEISGWVAMLEHNERHIRHTLPHLYELALGGTAVGTGLNTPP
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GFAQRIAAELAGQIGLPFVSAPNKFEALATCDALVQGHGALKGLACSLMKIANDVRWLAS
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
GPRCGIGELRLPENEPGSSIMPGKVNPTQCEALTMVCAQILGNDTAVNIGGASGNFELNV
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEH
FRPLVIHNILQSLRLLADGMESFNRHCAQGLMPNRERIDELLHRSLMLVTALAPHIGYDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHH
AAEIAKKAHREGTTLREAALTLGYVSAPAFDEWVQPAQMVGDSAQGEK
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]