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The map label for this gene is intE [H]

Identifier: 238919949

GI number: 238919949

Start: 1973860

End: 1974954

Strand: Direct

Name: intE [H]

Synonym: NT01EI_2053

Alternate gene names: 238919949

Gene position: 1973860-1974954 (Clockwise)

Preceding gene: 238919948

Following gene: 238919950

Centisome position: 51.78

GC content: 60.27

Gene sequence:

>1095_bases
ATGGTCCAAAATAACGATGACAGCGCTACGCCAAAATTACCGGACAACCTGTACTGTAAGGGCGGGTTTTTCATCTGGCG
CAATCCCCTCAGTGGGCAGGAGATGCCCCTGGGCTATATCAAAAAAAACGAGGCCGTTCGCCAGGCGCAAGAGGCCAACC
AGTATATCGACACCCAGCGACCGATGGTGGAAGCGTGCCCCAGCGTCAGCCAATGGATGGCGGAGTATCTGCGGATCCTC
GGCTACCGCGGCGTAGCCGCCAATACCCTGCGCGCCAGGCGCTCACAGATCAAGGCCGTACAAACGGTGATCGGGCAGAA
GAATATCGCTCAGGTCACCACCCGCGACATCGCCTTGCTGCTACACCACTATGTCCTGATCGGCCACCTTTCCGCCGCGG
GTCTGATGCGCTCCTTTCTCAACGCCCTATTTCGCGAGGCCATCGCCGCGGGCCTCATCGACTACAATCCGGTGACGCAG
ACCCGTACACCGCCGATCCGGGTCAAGCGCCGCCGCCTGACAGAAACGATGCTGCAGACCGTTTACGCGCAGGCGCTCCA
GCTGGAGCCGCATAAGCCATGGCTACCGCGCAGCATTGAACTGGCCCTGCTCACGGGGCAACGGCGGGAAGATCTCTGCG
CGCTCCGCTGGCAGGATATCCGGGAAGATAAACTGTGGGTGATCCAGCGCAAGACCGGCGCCAGACTGGCGATGACCCTC
GATCTCCATCTGCAGCTCGGTGACTATCCGCTGCGACTGCGCGAGGTACTGGCGCGCTGCCGGATACCGGGGCCGAGCGA
CTACCTGCTCAACTCCCAGCGCCCGCGCCTCAATAGGAGGCCGGGGGGATCTCTGGTGCCCGATACCCTGACCAAAGGTT
TCTCCCGGCTGATGGATAAGTGCGGACTGGTTCAAGAGATCTCCCCACCCTCTTTTCATGAGATCCGCAGTCTTTCCTCA
CGGATGTACCAGGCGCAGTATGGAACGGAATTCTGCCAGCGGCTGCTGGGGCATAAAAACCGGCATATGACCGAGAAATA
CCTCGATCTGAGGGAGGCACTCTGGCAGATGATAGAGCTCCCCAAAAACCGGTGA

Upstream 100 bases:

>100_bases
CGTATTCAGAGCGATGCCCGCTACCTGCCACCCTGGCAGCAGCCCACCGTTCCCGACGGCACGGCGGCGTCTACCCCCAC
CTTGCTGGAGAGGCTAAAAA

Downstream 100 bases:

>100_bases
TCGGGAGCCAGAATTGGCGGGAGACGCTTTAATGCCTGACACAGGAATCGTGACGGGTCTTTTTTTATTATAATAAAATC
AAATTATTACAAATATATTC

Product: integrase

Products: NA

Alternate protein names: Int(Lambda); Prophage e14 integrase [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MVQNNDDSATPKLPDNLYCKGGFFIWRNPLSGQEMPLGYIKKNEAVRQAQEANQYIDTQRPMVEACPSVSQWMAEYLRIL
GYRGVAANTLRARRSQIKAVQTVIGQKNIAQVTTRDIALLLHHYVLIGHLSAAGLMRSFLNALFREAIAAGLIDYNPVTQ
TRTPPIRVKRRRLTETMLQTVYAQALQLEPHKPWLPRSIELALLTGQRREDLCALRWQDIREDKLWVIQRKTGARLAMTL
DLHLQLGDYPLRLREVLARCRIPGPSDYLLNSQRPRLNRRPGGSLVPDTLTKGFSRLMDKCGLVQEISPPSFHEIRSLSS
RMYQAQYGTEFCQRLLGHKNRHMTEKYLDLREALWQMIELPKNR

Sequences:

>Translated_364_residues
MVQNNDDSATPKLPDNLYCKGGFFIWRNPLSGQEMPLGYIKKNEAVRQAQEANQYIDTQRPMVEACPSVSQWMAEYLRIL
GYRGVAANTLRARRSQIKAVQTVIGQKNIAQVTTRDIALLLHHYVLIGHLSAAGLMRSFLNALFREAIAAGLIDYNPVTQ
TRTPPIRVKRRRLTETMLQTVYAQALQLEPHKPWLPRSIELALLTGQRREDLCALRWQDIREDKLWVIQRKTGARLAMTL
DLHLQLGDYPLRLREVLARCRIPGPSDYLLNSQRPRLNRRPGGSLVPDTLTKGFSRLMDKCGLVQEISPPSFHEIRSLSS
RMYQAQYGTEFCQRLLGHKNRHMTEKYLDLREALWQMIELPKNR
>Mature_364_residues
MVQNNDDSATPKLPDNLYCKGGFFIWRNPLSGQEMPLGYIKKNEAVRQAQEANQYIDTQRPMVEACPSVSQWMAEYLRIL
GYRGVAANTLRARRSQIKAVQTVIGQKNIAQVTTRDIALLLHHYVLIGHLSAAGLMRSFLNALFREAIAAGLIDYNPVTQ
TRTPPIRVKRRRLTETMLQTVYAQALQLEPHKPWLPRSIELALLTGQRREDLCALRWQDIREDKLWVIQRKTGARLAMTL
DLHLQLGDYPLRLREVLARCRIPGPSDYLLNSQRPRLNRRPGGSLVPDTLTKGFSRLMDKCGLVQEISPPSFHEIRSLSS
RMYQAQYGTEFCQRLLGHKNRHMTEKYLDLREALWQMIELPKNR

Specific function: Integrase from the cryptic lambdoic prophage e14. Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the h

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1787386, Length=300, Percent_Identity=34, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016177
- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR015094
- InterPro:   IPR023109
- InterPro:   IPR013087 [H]

Pfam domain/function: PF09003 Phage_integ_N; PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 41937; Mature: 41937

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQNNDDSATPKLPDNLYCKGGFFIWRNPLSGQEMPLGYIKKNEAVRQAQEANQYIDTQR
CCCCCCCCCCCCCCCCCEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PMVEACPSVSQWMAEYLRILGYRGVAANTLRARRSQIKAVQTVIGQKNIAQVTTRDIALL
CHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LHHYVLIGHLSAAGLMRSFLNALFREAIAAGLIDYNPVTQTRTPPIRVKRRRLTETMLQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VYAQALQLEPHKPWLPRSIELALLTGQRREDLCALRWQDIREDKLWVIQRKTGARLAMTL
HHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEE
DLHLQLGDYPLRLREVLARCRIPGPSDYLLNSQRPRLNRRPGGSLVPDTLTKGFSRLMDK
EEEEEECCCCHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
CGLVQEISPPSFHEIRSLSSRMYQAQYGTEFCQRLLGHKNRHMTEKYLDLREALWQMIEL
CCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
PKNR
CCCC
>Mature Secondary Structure
MVQNNDDSATPKLPDNLYCKGGFFIWRNPLSGQEMPLGYIKKNEAVRQAQEANQYIDTQR
CCCCCCCCCCCCCCCCCEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PMVEACPSVSQWMAEYLRILGYRGVAANTLRARRSQIKAVQTVIGQKNIAQVTTRDIALL
CHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LHHYVLIGHLSAAGLMRSFLNALFREAIAAGLIDYNPVTQTRTPPIRVKRRRLTETMLQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VYAQALQLEPHKPWLPRSIELALLTGQRREDLCALRWQDIREDKLWVIQRKTGARLAMTL
HHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEE
DLHLQLGDYPLRLREVLARCRIPGPSDYLLNSQRPRLNRRPGGSLVPDTLTKGFSRLMDK
EEEEEECCCCHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
CGLVQEISPPSFHEIRSLSSRMYQAQYGTEFCQRLLGHKNRHMTEKYLDLREALWQMIEL
CCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
PKNR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]