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The map label for this gene is bmr3 [H]

Identifier: 238919883

GI number: 238919883

Start: 1911434

End: 1912912

Strand: Reverse

Name: bmr3 [H]

Synonym: NT01EI_1987

Alternate gene names: 238919883

Gene position: 1912912-1911434 (Counterclockwise)

Preceding gene: 238919884

Following gene: 238919878

Centisome position: 50.18

GC content: 59.77

Gene sequence:

>1479_bases
ATGAGTACGGATACGACACCGATAAGCCCGAAGCGGGTACTGGTCTTGACAGCCTGCATGCTTGCCACCTTCATGGCTGC
GATTGAGGTAACGATTGTCTCTACCGCCATGCCAACCATCATTGCCGATTTGGGCGGCTTTTCCCTGCTGGGGTGGGTGT
TTGCCGCCTATTTGCTGACACAGGCGATCTCTATCCCTATCTATGGGCGGTTAGCAGATCTGCATGGCCGCAAGCGGATG
TTTTATATTGGTGCCTCGCTGTTCCTTCTGGGCTCGGTGCTCTGCGGTTTCGCCCACAGTATGTTGTGGCTGATCGCGTT
CCGCGCGCTGCAGGGGGCCGGTGCCGGGGCGATTACGCCGATCGCTGTCACCATTATCGCCGATGTTTATGGTCCCCTTG
AACGTCCCCGTATTCAGGGATATCTCTCCAGCGTCTGGGGCGTATCGGCGATCGTCGGGCCATTGATGGGGGCATTTATC
GTTCAGCATTTCCACTGGTCCCTGGTCTTTTGGATCAATTTGCCCATTGGCTGCTGCGCGATGTATTTGCTATGGCGCTA
TTTTCCCGAACAACGGCATCCGCGGCGCCATACGCTGGATCTGGCTGGAACCGCCTGGCTCGCCATTGGTATCAGCGCGC
TGTTGCTATCACTGCTTCAGGCCGAGTGGCTGGGGCTGTGGGTGCTGCTGCTGCTGGCCATTGCCGTGCTGAGCGGTTTT
TTACTGTGGCGTCAGGAGTTGCGCGCGGCAGAGCCCCTGTTCCCGGCCGTACTCTGGCGCCAGCGCATCATGGTGGCCGG
TAACATCGGCGGGCTGGTCGTTGGGGCCGCCATGATGGGGGTTGCTGCATTTTTGCCCACCTATGTGCAGGGCGTCATGG
GGTATTCGGCTCTGGAAGCGGGCACAACGCTGGCTCTGATGTCGATTGGCTGGCCTATCGCGAGCACACTCAGTGGTCGG
CTGATGGTCTATACGTCATACCGGACCACGGCGCTACTGGGCGCCCTGTTGCTGATCGCGGGAAGCCTGTGTCTGCTGTG
GCAGACTCCGCAGGGGAGCCTGTGGTGGGGGCGTATTTCGGCCTTCATCATCGGTGCGGCGATGGGAATGACCAATACCA
CCTTCCTGGTCTCCGTCCAGAACAGTGCGCCGCACTCCATGCGCGGTATTGCGACGGCCTGCACGGTATTTACCCGAATG
CTTGGCTCTGCGCTGGGCACTGCAATTCTCGGCGCGACGCTAAATCTGAATTTGGCCTGGCGCCTACCGCAGATAAACGA
TCCGGTACAGCAGTTGATGACTTTGCGTACCCAGACTGCACCGGAGGACGTCGGCGCGCTGCTGGCGCCGGTTGCAGCAT
CGTTGCACGCGGTGTTTATTGCTGCGGCACTATTGGCGTGGCTTGCCCTGCTGGCAGCATGGCTGATCCCGGCGGGCATA
GGGCTGCGGGAGCGGAGAGAAGAGGTTGTTGAGAAGTAA

Upstream 100 bases:

>100_bases
TGGTAATGACGGCGGAAAGGTGCAGGCAAGGAAAGTAAGTGGGAATCGTCTTATCTCTCCATGTTAGTATAGTCGCAGGA
TTTTCTATGACATAGGCCCT

Downstream 100 bases:

>100_bases
TTCAGGCCGCCTTCGGCGGCCTGAATTACTTAGCGTGGATTCGCGTAGGCGCCGGGCTCGGCGCCGTCGGTACGAATATA
GATAATTTTCTGTGTGTCGT

Product: hypothetical protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 3 [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MSTDTTPISPKRVLVLTACMLATFMAAIEVTIVSTAMPTIIADLGGFSLLGWVFAAYLLTQAISIPIYGRLADLHGRKRM
FYIGASLFLLGSVLCGFAHSMLWLIAFRALQGAGAGAITPIAVTIIADVYGPLERPRIQGYLSSVWGVSAIVGPLMGAFI
VQHFHWSLVFWINLPIGCCAMYLLWRYFPEQRHPRRHTLDLAGTAWLAIGISALLLSLLQAEWLGLWVLLLLAIAVLSGF
LLWRQELRAAEPLFPAVLWRQRIMVAGNIGGLVVGAAMMGVAAFLPTYVQGVMGYSALEAGTTLALMSIGWPIASTLSGR
LMVYTSYRTTALLGALLLIAGSLCLLWQTPQGSLWWGRISAFIIGAAMGMTNTTFLVSVQNSAPHSMRGIATACTVFTRM
LGSALGTAILGATLNLNLAWRLPQINDPVQQLMTLRTQTAPEDVGALLAPVAASLHAVFIAAALLAWLALLAAWLIPAGI
GLRERREEVVEK

Sequences:

>Translated_492_residues
MSTDTTPISPKRVLVLTACMLATFMAAIEVTIVSTAMPTIIADLGGFSLLGWVFAAYLLTQAISIPIYGRLADLHGRKRM
FYIGASLFLLGSVLCGFAHSMLWLIAFRALQGAGAGAITPIAVTIIADVYGPLERPRIQGYLSSVWGVSAIVGPLMGAFI
VQHFHWSLVFWINLPIGCCAMYLLWRYFPEQRHPRRHTLDLAGTAWLAIGISALLLSLLQAEWLGLWVLLLLAIAVLSGF
LLWRQELRAAEPLFPAVLWRQRIMVAGNIGGLVVGAAMMGVAAFLPTYVQGVMGYSALEAGTTLALMSIGWPIASTLSGR
LMVYTSYRTTALLGALLLIAGSLCLLWQTPQGSLWWGRISAFIIGAAMGMTNTTFLVSVQNSAPHSMRGIATACTVFTRM
LGSALGTAILGATLNLNLAWRLPQINDPVQQLMTLRTQTAPEDVGALLAPVAASLHAVFIAAALLAWLALLAAWLIPAGI
GLRERREEVVEK
>Mature_491_residues
STDTTPISPKRVLVLTACMLATFMAAIEVTIVSTAMPTIIADLGGFSLLGWVFAAYLLTQAISIPIYGRLADLHGRKRMF
YIGASLFLLGSVLCGFAHSMLWLIAFRALQGAGAGAITPIAVTIIADVYGPLERPRIQGYLSSVWGVSAIVGPLMGAFIV
QHFHWSLVFWINLPIGCCAMYLLWRYFPEQRHPRRHTLDLAGTAWLAIGISALLLSLLQAEWLGLWVLLLLAIAVLSGFL
LWRQELRAAEPLFPAVLWRQRIMVAGNIGGLVVGAAMMGVAAFLPTYVQGVMGYSALEAGTTLALMSIGWPIASTLSGRL
MVYTSYRTTALLGALLLIAGSLCLLWQTPQGSLWWGRISAFIIGAAMGMTNTTFLVSVQNSAPHSMRGIATACTVFTRML
GSALGTAILGATLNLNLAWRLPQINDPVQQLMTLRTQTAPEDVGALLAPVAASLHAVFIAAALLAWLALLAAWLIPAGIG
LRERREEVVEK

Specific function: Confers resistance to puromycin, tosufloxacin and norfloxacin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=407, Percent_Identity=28.2555282555283, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1789042, Length=424, Percent_Identity=29.7169811320755, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87081983, Length=424, Percent_Identity=29.0094339622642, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1788710, Length=343, Percent_Identity=28.2798833819242, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1788392, Length=295, Percent_Identity=28.4745762711864, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1790146, Length=207, Percent_Identity=27.536231884058, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322958, Length=204, Percent_Identity=31.3725490196078, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6319770, Length=395, Percent_Identity=26.8354430379747, Blast_Score=93, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6323735, Length=310, Percent_Identity=25.8064516129032, Blast_Score=90, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6321663, Length=172, Percent_Identity=28.4883720930233, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=178, Percent_Identity=29.2134831460674, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324954, Length=367, Percent_Identity=25.6130790190736, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52969; Mature: 52838

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDTTPISPKRVLVLTACMLATFMAAIEVTIVSTAMPTIIADLGGFSLLGWVFAAYLLT
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
QAISIPIYGRLADLHGRKRMFYIGASLFLLGSVLCGFAHSMLWLIAFRALQGAGAGAITP
HHHCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
IAVTIIADVYGPLERPRIQGYLSSVWGVSAIVGPLMGAFIVQHFHWSLVFWINLPIGCCA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHH
MYLLWRYFPEQRHPRRHTLDLAGTAWLAIGISALLLSLLQAEWLGLWVLLLLAIAVLSGF
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLWRQELRAAEPLFPAVLWRQRIMVAGNIGGLVVGAAMMGVAAFLPTYVQGVMGYSALEA
HHHHHHHHHCCCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GTTLALMSIGWPIASTLSGRLMVYTSYRTTALLGALLLIAGSLCLLWQTPQGSLWWGRIS
CCHHHHHHHCCCHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
AFIIGAAMGMTNTTFLVSVQNSAPHSMRGIATACTVFTRMLGSALGTAILGATLNLNLAW
HHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEE
RLPQINDPVQQLMTLRTQTAPEDVGALLAPVAASLHAVFIAAALLAWLALLAAWLIPAGI
ECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GLRERREEVVEK
CHHHHHHHHHCC
>Mature Secondary Structure 
STDTTPISPKRVLVLTACMLATFMAAIEVTIVSTAMPTIIADLGGFSLLGWVFAAYLLT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH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CHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023234; 9384377 [H]