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The map label for this gene is ttcA [H]

Identifier: 238919821

GI number: 238919821

Start: 1854192

End: 1855190

Strand: Reverse

Name: ttcA [H]

Synonym: NT01EI_1925

Alternate gene names: 238919821

Gene position: 1855190-1854192 (Counterclockwise)

Preceding gene: 238919824

Following gene: 238919817

Centisome position: 48.66

GC content: 58.26

Gene sequence:

>999_bases
ATGATTGCGCCGTCGCCCAAGGCGCCGCCGCAACCCTTTTGTCTATGCCGGGTAACAGAAACGATGTCAGAGAATCAAGA
GATCAGCAAAAAAGAGCAGTACAACCTGAACAAACTGCAGAAGCGCCTGCGCCGCTGCGTCGGTGAAGCCATCGCCGACT
TCAATATGATCGAAGAGGGCGATCGGGTGATGGTTTGCCTGTCCGGCGGCAAAGACAGCTATACCATGCTGGAGATCCTG
CGTAACCTGCAGCAGAGCGCACCGGTGGACTTTACCCTGGTCGCGGTCAACCTTGACCAGAAACAGCCCGGCTTCCCGGA
GACCGTTCTGCCGGCCTACCTGGAGAACCAGGGCATCGAATACCGCATCGTCGAAGAGAATACCTACGGTATCGTCAAGG
AGAAAATTCCGGAGGGAAAAACCACCTGCTCTCTCTGTTCTCGCCTGCGCCGCGGCATTCTCTATCGCACCGCCAACGAG
CTGCACTGCAATAAGATCGCGCTGGGTCACCACCGTGACGACATGCTGGAAACCCTGTTTCTCAACATGTTCTACGGCGG
CAAGCTCAAAGGGATGCCCCCCAAGCTGATGAGCGATGACGGCCAGCATATTGTTATCCGACCACTCGCCTACTGCCGCG
AAAAGGACATTGAGCGCTTCGCCGCCGCCAAGGCTTTCCCGATTATTCCGTGTAATCTGTGCGGCTCCCAGCCTAACCTG
CAGCGCCAGGTGATTAAAGAGATGCTGCGCGACTGGGACAAGCGCTATCCGGGGCGCATCGAAACCATGTTCCGCGCCAT
GCAGAACGTGGTGCCGTCGCATCTGGCGGACGGTGCCCTGTTTGACTTTAAGGGACTGCTGCAGGGCGGCGAGATTATCG
ACGGCGGCGATTTGGCCTTCGATCGCCAGATAATTCCGCTGCAGCCGGTCGGCCTGAGCGACGACGCGGATCGTCCACAG
ACGGACGGTATGCGCCTGGACGTCGTCGAGGTGAAGTGA

Upstream 100 bases:

>100_bases
TCCTCTCGCCCCTGAAGCGTCGGTGCCACATAATTCCTGCTGGCATTCGCCGCCGTAATCCGTACCATACACGCCACGCC
GTCCACGATGACAGCCCCCG

Downstream 100 bases:

>100_bases
TCTCCCCCTGCCGGGCGTCTGGAGGCCCGCAGGATTCAGAGATAAAAAAAATGCCGGTAATCACCGGCATATGACGAATT
AAATTGTGCTATGCAATAAT

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MIAPSPKAPPQPFCLCRVTETMSENQEISKKEQYNLNKLQKRLRRCVGEAIADFNMIEEGDRVMVCLSGGKDSYTMLEIL
RNLQQSAPVDFTLVAVNLDQKQPGFPETVLPAYLENQGIEYRIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTANE
LHCNKIALGHHRDDMLETLFLNMFYGGKLKGMPPKLMSDDGQHIVIRPLAYCREKDIERFAAAKAFPIIPCNLCGSQPNL
QRQVIKEMLRDWDKRYPGRIETMFRAMQNVVPSHLADGALFDFKGLLQGGEIIDGGDLAFDRQIIPLQPVGLSDDADRPQ
TDGMRLDVVEVK

Sequences:

>Translated_332_residues
MIAPSPKAPPQPFCLCRVTETMSENQEISKKEQYNLNKLQKRLRRCVGEAIADFNMIEEGDRVMVCLSGGKDSYTMLEIL
RNLQQSAPVDFTLVAVNLDQKQPGFPETVLPAYLENQGIEYRIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTANE
LHCNKIALGHHRDDMLETLFLNMFYGGKLKGMPPKLMSDDGQHIVIRPLAYCREKDIERFAAAKAFPIIPCNLCGSQPNL
QRQVIKEMLRDWDKRYPGRIETMFRAMQNVVPSHLADGALFDFKGLLQGGEIIDGGDLAFDRQIIPLQPVGLSDDADRPQ
TDGMRLDVVEVK
>Mature_332_residues
MIAPSPKAPPQPFCLCRVTETMSENQEISKKEQYNLNKLQKRLRRCVGEAIADFNMIEEGDRVMVCLSGGKDSYTMLEIL
RNLQQSAPVDFTLVAVNLDQKQPGFPETVLPAYLENQGIEYRIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTANE
LHCNKIALGHHRDDMLETLFLNMFYGGKLKGMPPKLMSDDGQHIVIRPLAYCREKDIERFAAAKAFPIIPCNLCGSQPNL
QRQVIKEMLRDWDKRYPGRIETMFRAMQNVVPSHLADGALFDFKGLLQGGEIIDGGDLAFDRQIIPLQPVGLSDDADRPQ
TDGMRLDVVEVK

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=311, Percent_Identity=83.2797427652733, Blast_Score=545, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI193211090, Length=271, Percent_Identity=31.3653136531365, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17540090, Length=234, Percent_Identity=27.7777777777778, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24651972, Length=225, Percent_Identity=27.1111111111111, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 37562; Mature: 37562

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAPSPKAPPQPFCLCRVTETMSENQEISKKEQYNLNKLQKRLRRCVGEAIADFNMIEEG
CCCCCCCCCCCCEEEEEEHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
DRVMVCLSGGKDSYTMLEILRNLQQSAPVDFTLVAVNLDQKQPGFPETVLPAYLENQGIE
CEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCE
YRIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTANELHCNKIALGHHRDDMLETLF
EEEEECCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHH
LNMFYGGKLKGMPPKLMSDDGQHIVIRPLAYCREKDIERFAAAKAFPIIPCNLCGSQPNL
HHHHCCCCCCCCCCHHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEHHCCCCCCCH
QRQVIKEMLRDWDKRYPGRIETMFRAMQNVVPSHLADGALFDFKGLLQGGEIIDGGDLAF
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEECCCCCEE
DRQIIPLQPVGLSDDADRPQTDGMRLDVVEVK
CCEECCCCCCCCCCCCCCCCCCCEEEEEEEEC
>Mature Secondary Structure
MIAPSPKAPPQPFCLCRVTETMSENQEISKKEQYNLNKLQKRLRRCVGEAIADFNMIEEG
CCCCCCCCCCCCEEEEEEHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
DRVMVCLSGGKDSYTMLEILRNLQQSAPVDFTLVAVNLDQKQPGFPETVLPAYLENQGIE
CEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCE
YRIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTANELHCNKIALGHHRDDMLETLF
EEEEECCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHH
LNMFYGGKLKGMPPKLMSDDGQHIVIRPLAYCREKDIERFAAAKAFPIIPCNLCGSQPNL
HHHHCCCCCCCCCCHHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEHHCCCCCCCH
QRQVIKEMLRDWDKRYPGRIETMFRAMQNVVPSHLADGALFDFKGLLQGGEIIDGGDLAF
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEECCCCCEE
DRQIIPLQPVGLSDDADRPQTDGMRLDVVEVK
CCEECCCCCCCCCCCCCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA