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The map label for this gene is btuD [H]

Identifier: 238919791

GI number: 238919791

Start: 1824230

End: 1825024

Strand: Direct

Name: btuD [H]

Synonym: NT01EI_1895

Alternate gene names: 238919791

Gene position: 1824230-1825024 (Clockwise)

Preceding gene: 238919790

Following gene: 238919797

Centisome position: 47.85

GC content: 60.13

Gene sequence:

>795_bases
ATGTACGATAATGGGCTGCGTATCGATGGTGTGGCAGTTTCCGGCCGGTTGGCGCCCCTGAGTGCGTTGGTGGCGCGCGG
ACGGTTGGTGCATATCATCGGCCCGAACGGTGCGGGTAAAAGCACACTGTTGCTGCGGTTAGCCGGTTTATTGAGCGGGG
AAGGCGAGGTATGGCTGGCGGGGCGGCCGCTGCTGTGCCAGACTCCCGCACAGCAGGCGCGTTGGCGCGCGTGTCTGCTA
CAGCAACAGCTACCGACCGGGCTTATGCCTGTATTTGCTTATCTGGGACTACATGCGCCGCGTACTGCCCCTATCTCGGG
GGTCGATACCGTTCTGGCGATGCTGAGTGAGTGTCTGGGGCTCAGTGACAAACTGAGTCGGCCGATGAGCCATTTGTCCG
GTGGTGAGTGGCAGCGGGTACGGCTGGCGGCTGTGCTATTGCAAATTTGGCCGACCTTAAATCCGCAGGGGTATCTGCTG
CTGCTGGATGAGCCGATGAGCAGCCTGGATATCCGGCAGCAGGCCGCATTGGATGGCCTGCTGAGTGAACTGTGCGCTGC
TGGGGTTACTGTGATTGCCAGTGCGCACGATCTTAATCACACGCTATTCCATGCCGACGATGTCTGGATGCTTAGCCAGG
GGCAGGTGATGGCGCAGGGCACGGCGCAGGCCGTGATGCAGGTGGCGGCCCTGAGTACGTTGTTTGGCGTACCTTTTCGC
CGTATCCGCGGCGGCGACCGTGACTGGCTCTATTATCACAATGATGAACAGCCTGAAAGCGCATCGGGAAAATGA

Upstream 100 bases:

>100_bases
GCACTGTTTTCTGCCGAGTTGCCGATCGGGGTGGTGACCTCATCAATTGGTGCACCGCTGTTTATCTGGATGCTACTGCG
GCGCTCATCAGGGGGAATCA

Downstream 100 bases:

>100_bases
AGCCCAATAAGCATATCCACACTGATAGCTGCCACCATATCATCAGCGTATGAAAAACGATCATGTGACATGATAAATCC
CTGTATTTTCTAGATGCCGG

Product: vitamin B12 import ATP-binding protein BtuD

Products: NA

Alternate protein names: Vitamin B12-transporting ATPase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MYDNGLRIDGVAVSGRLAPLSALVARGRLVHIIGPNGAGKSTLLLRLAGLLSGEGEVWLAGRPLLCQTPAQQARWRACLL
QQQLPTGLMPVFAYLGLHAPRTAPISGVDTVLAMLSECLGLSDKLSRPMSHLSGGEWQRVRLAAVLLQIWPTLNPQGYLL
LLDEPMSSLDIRQQAALDGLLSELCAAGVTVIASAHDLNHTLFHADDVWMLSQGQVMAQGTAQAVMQVAALSTLFGVPFR
RIRGGDRDWLYYHNDEQPESASGK

Sequences:

>Translated_264_residues
MYDNGLRIDGVAVSGRLAPLSALVARGRLVHIIGPNGAGKSTLLLRLAGLLSGEGEVWLAGRPLLCQTPAQQARWRACLL
QQQLPTGLMPVFAYLGLHAPRTAPISGVDTVLAMLSECLGLSDKLSRPMSHLSGGEWQRVRLAAVLLQIWPTLNPQGYLL
LLDEPMSSLDIRQQAALDGLLSELCAAGVTVIASAHDLNHTLFHADDVWMLSQGQVMAQGTAQAVMQVAALSTLFGVPFR
RIRGGDRDWLYYHNDEQPESASGK
>Mature_264_residues
MYDNGLRIDGVAVSGRLAPLSALVARGRLVHIIGPNGAGKSTLLLRLAGLLSGEGEVWLAGRPLLCQTPAQQARWRACLL
QQQLPTGLMPVFAYLGLHAPRTAPISGVDTVLAMLSECLGLSDKLSRPMSHLSGGEWQRVRLAAVLLQIWPTLNPQGYLL
LLDEPMSSLDIRQQAALDGLLSELCAAGVTVIASAHDLNHTLFHADDVWMLSQGQVMAQGTAQAVMQVAALSTLFGVPFR
RIRGGDRDWLYYHNDEQPESASGK

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Responsible for energy coupling to the transport system [H]

COG id: COG4138

COG function: function code H; ABC-type cobalamin transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI215820660, Length=260, Percent_Identity=30.3846153846154, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI20143975, Length=260, Percent_Identity=30.3846153846154, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1788002, Length=237, Percent_Identity=48.9451476793249, Blast_Score=215, Evalue=2e-57,
Organism=Escherichia coli, GI1790739, Length=245, Percent_Identity=35.1020408163265, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786345, Length=250, Percent_Identity=33.6, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786803, Length=225, Percent_Identity=31.1111111111111, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788165, Length=250, Percent_Identity=29.2, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081709, Length=209, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789586, Length=216, Percent_Identity=32.8703703703704, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786563, Length=202, Percent_Identity=30.6930693069307, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081834, Length=200, Percent_Identity=33.5, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787370, Length=228, Percent_Identity=25.8771929824561, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786253, Length=222, Percent_Identity=28.8288288288288, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789962, Length=218, Percent_Identity=27.5229357798165, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1786398, Length=217, Percent_Identity=30.4147465437788, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17541710, Length=223, Percent_Identity=28.6995515695067, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI85724858, Length=211, Percent_Identity=32.2274881516588, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI85724856, Length=211, Percent_Identity=32.2274881516588, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24580547, Length=211, Percent_Identity=32.2274881516588, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24580551, Length=211, Percent_Identity=32.2274881516588, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24580549, Length=211, Percent_Identity=32.2274881516588, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24580545, Length=211, Percent_Identity=32.2274881516588, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19920532, Length=216, Percent_Identity=27.7777777777778, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24580930, Length=216, Percent_Identity=27.7777777777778, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI161076682, Length=230, Percent_Identity=30.4347826086957, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI161076684, Length=230, Percent_Identity=30.4347826086957, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24581617, Length=230, Percent_Identity=30.4347826086957, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24581615, Length=230, Percent_Identity=30.4347826086957, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: =3.6.3.33 [H]

Molecular weight: Translated: 28445; Mature: 28445

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDNGLRIDGVAVSGRLAPLSALVARGRLVHIIGPNGAGKSTLLLRLAGLLSGEGEVWLA
CCCCCEEEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEC
GRPLLCQTPAQQARWRACLLQQQLPTGLMPVFAYLGLHAPRTAPISGVDTVLAMLSECLG
CCCCEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
LSDKLSRPMSHLSGGEWQRVRLAAVLLQIWPTLNPQGYLLLLDEPMSSLDIRQQAALDGL
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
LSELCAAGVTVIASAHDLNHTLFHADDVWMLSQGQVMAQGTAQAVMQVAALSTLFGVPFR
HHHHHHCCHHEEEECCCCCCEEEECCCEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCHH
RIRGGDRDWLYYHNDEQPESASGK
HHCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MYDNGLRIDGVAVSGRLAPLSALVARGRLVHIIGPNGAGKSTLLLRLAGLLSGEGEVWLA
CCCCCEEEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEC
GRPLLCQTPAQQARWRACLLQQQLPTGLMPVFAYLGLHAPRTAPISGVDTVLAMLSECLG
CCCCEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
LSDKLSRPMSHLSGGEWQRVRLAAVLLQIWPTLNPQGYLLLLDEPMSSLDIRQQAALDGL
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
LSELCAAGVTVIASAHDLNHTLFHADDVWMLSQGQVMAQGTAQAVMQVAALSTLFGVPFR
HHHHHHCCHHEEEECCCCCCEEEECCCEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCHH
RIRGGDRDWLYYHNDEQPESASGK
HHCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]