Click here to switch to the map view.

The map label for this gene is ribE [H]

Identifier: 238919628

GI number: 238919628

Start: 1683307

End: 1683942

Strand: Direct

Name: ribE [H]

Synonym: NT01EI_1729

Alternate gene names: 238919628

Gene position: 1683307-1683942 (Clockwise)

Preceding gene: 238919626

Following gene: 238919630

Centisome position: 44.15

GC content: 54.87

Gene sequence:

>636_bases
ATGTTTACCGGTATTGTTCAGGGCACCGCATCGGTGGTGGCGATCGATGAAAAAACCAATTTCCGTACCCACATAGTACA
GTTGCCGCCTGATATGTTGCCGGGATTGGCGCTGGGTGCTTCGGTGGCCAATAACGGCGTGTGTCTGACCGTCACCGGGA
TTGAGGATGATCGGGTCAGCTTTGACCTGATGAAAGAGACGCTGCGGGTGACGAATCTGGGAAGCCTGCGGGTGGGTGAT
CGGGTTAACCTGGAGCGGGCCGCCAAGTTTAATGATGAGATCGGCGGCCATTTGATGTCCGGTCATGTTATGGGGATGGC
GGAGATCGTCAAAATTCTCAGTTCAGAGCACAATCGCCAGATTTGGTTCCGTATCAGCGACAGTGAAATGATGAAATATG
TGCTGCATAAAGGGTTTATCGGCATTGACGGTATCAGTCTGACCCTCGGTGAGGTGAGTAAGACCCGCTTCTGCGTACAT
CTAATCCCGGAGACCCTGCAGCGTACCACGCTGGGAGGGAAGCGCCTGGGTGATTGGGTAAATATTGAAATCGACCCGCA
GACTCAGGCGGTCGTCGATACCGTTGAGCGGGTGTTGGCCAGCCGTGCTGCCGTTGACGGCGCTCAGTCTGCCTGA

Upstream 100 bases:

>100_bases
AAATGGGGTCTATAGCGTAATACTTTGGTTTACGAATGATTTAGGGCAAACTCATAGACAGTAATCCTAACTGAATATCT
CATATATAGAGGAAGGCATT

Downstream 100 bases:

>100_bases
CCTCCGCGGGCTAGCGCTCTGCGTGGGCGAGTCCGCGGGTGCGCATCATGCTGATAATTGCGGCCAGCGCGGCGCATGCG
GCGCCCAGCAGCAGGGCGCC

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MFTGIVQGTASVVAIDEKTNFRTHIVQLPPDMLPGLALGASVANNGVCLTVTGIEDDRVSFDLMKETLRVTNLGSLRVGD
RVNLERAAKFNDEIGGHLMSGHVMGMAEIVKILSSEHNRQIWFRISDSEMMKYVLHKGFIGIDGISLTLGEVSKTRFCVH
LIPETLQRTTLGGKRLGDWVNIEIDPQTQAVVDTVERVLASRAAVDGAQSA

Sequences:

>Translated_211_residues
MFTGIVQGTASVVAIDEKTNFRTHIVQLPPDMLPGLALGASVANNGVCLTVTGIEDDRVSFDLMKETLRVTNLGSLRVGD
RVNLERAAKFNDEIGGHLMSGHVMGMAEIVKILSSEHNRQIWFRISDSEMMKYVLHKGFIGIDGISLTLGEVSKTRFCVH
LIPETLQRTTLGGKRLGDWVNIEIDPQTQAVVDTVERVLASRAAVDGAQSA
>Mature_211_residues
MFTGIVQGTASVVAIDEKTNFRTHIVQLPPDMLPGLALGASVANNGVCLTVTGIEDDRVSFDLMKETLRVTNLGSLRVGD
RVNLERAAKFNDEIGGHLMSGHVMGMAEIVKILSSEHNRQIWFRISDSEMMKYVLHKGFIGIDGISLTLGEVSKTRFCVH
LIPETLQRTTLGGKRLGDWVNIEIDPQTQAVVDTVERVLASRAAVDGAQSA

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=202, Percent_Identity=76.7326732673267, Blast_Score=325, Evalue=1e-90,
Organism=Saccharomyces cerevisiae, GI6319733, Length=196, Percent_Identity=35.7142857142857, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 22986; Mature: 22986

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIVQGTASVVAIDEKTNFRTHIVQLPPDMLPGLALGASVANNGVCLTVTGIEDDRVS
CCCCEECCCEEEEEEECCCCCEEEEEECCHHHCCCHHHHHHHCCCCEEEEEECCCCCHHH
FDLMKETLRVTNLGSLRVGDRVNLERAAKFNDEIGGHLMSGHVMGMAEIVKILSSEHNRQ
HHHHHHHHHHCCCCCEECCCCCCHHHHHHCCHHCCCEEECCHHHHHHHHHHHHHCCCCCE
IWFRISDSEMMKYVLHKGFIGIDGISLTLGEVSKTRFCVHLIPETLQRTTLGGKRLGDWV
EEEEECHHHHHHHHHHCCCEECCCCEEEECCCCHHHHHEEHHHHHHHHHCCCCCCCCCEE
NIEIDPQTQAVVDTVERVLASRAAVDGAQSA
EEEECCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFTGIVQGTASVVAIDEKTNFRTHIVQLPPDMLPGLALGASVANNGVCLTVTGIEDDRVS
CCCCEECCCEEEEEEECCCCCEEEEEECCHHHCCCHHHHHHHCCCCEEEEEECCCCCHHH
FDLMKETLRVTNLGSLRVGDRVNLERAAKFNDEIGGHLMSGHVMGMAEIVKILSSEHNRQ
HHHHHHHHHHCCCCCEECCCCCCHHHHHHCCHHCCCEEECCHHHHHHHHHHHHHCCCCCE
IWFRISDSEMMKYVLHKGFIGIDGISLTLGEVSKTRFCVHLIPETLQRTTLGGKRLGDWV
EEEEECHHHHHHHHHHCCCEECCCCEEEECCCCHHHHHEEHHHHHHHHHCCCCCCCCCEE
NIEIDPQTQAVVDTVERVLASRAAVDGAQSA
EEEECCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9022701; 9023191; 9097039; 9278503; 11377200 [H]