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The map label for this gene is xthA [H]

Identifier: 238919557

GI number: 238919557

Start: 1615229

End: 1616038

Strand: Reverse

Name: xthA [H]

Synonym: NT01EI_1653

Alternate gene names: 238919557

Gene position: 1616038-1615229 (Counterclockwise)

Preceding gene: 238919558

Following gene: 238919556

Centisome position: 42.39

GC content: 59.26

Gene sequence:

>810_bases
ATGAAGTTTATCTCTTTCAACATCAACGGGCTGCGTGCCCGCCCCCATCAGCTCGCGGCGATCGTTGAGAAGCATAGCCC
GGACGTGATCGGTCTGCAGGAAACCAAGGTACATGACGATATGTTTCCGCTGCAGGATCTGGCACATCTGGGGTATCAGC
TGCACTACCATGGCCAGAAAGGCCACTACGGCGTCGCCCTGCTGACCCGAATGGCACCGTTGGCGGTGCGTAAAGGCTTC
CCGGGTGACGATGCCGAAGCACAGCGGCGCGTCATCATGGCGGATATCCCCACCGAGCTGGGCACGCTGACCGTCATTAA
CGGCTACTTTCCGCAGGGCGAAAGTCGCGATCACCCGATAAAGTTTCCGGCAAAGAGCAAATTCTACGCCGATCTGCAAC
GCTACCTGCAGGATTCACTCACGCCGCAGAACCCGGTGCTAATCATGGGTGATATGAACATCAGCCCACGCGATATTGAC
ATCGGGATCGGTGATGAGAGTCGACGGCGCTGGCTGCGCAGCGGCAAGTGCTCCTTCCTGCCGGAGGAGCGGGAGTGGCT
GGATCGGCTGCTGGCGTGGGGTCTGGTCGATACCTACCGTCAGGCCAACCCGCAGGGTGACGACCATTTCTCGTGGTTTG
ACTACCGCTCCAAAGGGTTTGATGATAACCGCGGTCTACGCATCGATCTGCTGCTGGCCAGTCAGCCGCTGGCCCAGCGC
TGTACCGATACCGGCATCGACTATGAGATCCGCGCCATGGAGAAACCCTCGGATCACGCCCCGATCTGGGCCGAGTTCGC
ACTTTCATGA

Upstream 100 bases:

>100_bases
CTGAACGCCCTATCGCCCGATTACCCCCACGCAGGACAAGGTTCAGCTTGCGACCGCCATCTGCTACCATGCGGGCACCT
TTATTCAACGGCCAGAATCC

Downstream 100 bases:

>100_bases
GCGCGCAGGCAGCGGAAAAAATCCTGCATGTCGTCGCCGCCATCATTGAGCGGCGCGGTGCTATCCTGCTAGCTCAGCGC
GGCCAGGGGCAGGATCAGGC

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MKFISFNINGLRARPHQLAAIVEKHSPDVIGLQETKVHDDMFPLQDLAHLGYQLHYHGQKGHYGVALLTRMAPLAVRKGF
PGDDAEAQRRVIMADIPTELGTLTVINGYFPQGESRDHPIKFPAKSKFYADLQRYLQDSLTPQNPVLIMGDMNISPRDID
IGIGDESRRRWLRSGKCSFLPEEREWLDRLLAWGLVDTYRQANPQGDDHFSWFDYRSKGFDDNRGLRIDLLLASQPLAQR
CTDTGIDYEIRAMEKPSDHAPIWAEFALS

Sequences:

>Translated_269_residues
MKFISFNINGLRARPHQLAAIVEKHSPDVIGLQETKVHDDMFPLQDLAHLGYQLHYHGQKGHYGVALLTRMAPLAVRKGF
PGDDAEAQRRVIMADIPTELGTLTVINGYFPQGESRDHPIKFPAKSKFYADLQRYLQDSLTPQNPVLIMGDMNISPRDID
IGIGDESRRRWLRSGKCSFLPEEREWLDRLLAWGLVDTYRQANPQGDDHFSWFDYRSKGFDDNRGLRIDLLLASQPLAQR
CTDTGIDYEIRAMEKPSDHAPIWAEFALS
>Mature_269_residues
MKFISFNINGLRARPHQLAAIVEKHSPDVIGLQETKVHDDMFPLQDLAHLGYQLHYHGQKGHYGVALLTRMAPLAVRKGF
PGDDAEAQRRVIMADIPTELGTLTVINGYFPQGESRDHPIKFPAKSKFYADLQRYLQDSLTPQNPVLIMGDMNISPRDID
IGIGDESRRRWLRSGKCSFLPEEREWLDRLLAWGLVDTYRQANPQGDDHFSWFDYRSKGFDDNRGLRIDLLLASQPLAQR
CTDTGIDYEIRAMEKPSDHAPIWAEFALS

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=274, Percent_Identity=27.3722627737226, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI18375503, Length=274, Percent_Identity=27.3722627737226, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI18375501, Length=274, Percent_Identity=27.3722627737226, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=79.6992481203008, Blast_Score=465, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI71989536, Length=267, Percent_Identity=23.5955056179775, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI221330655, Length=265, Percent_Identity=29.4339622641509, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17136678, Length=265, Percent_Identity=29.4339622641509, Blast_Score=91, Evalue=7e-19,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30716; Mature: 30716

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00727 AP_NUCLEASE_F1_2 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFISFNINGLRARPHQLAAIVEKHSPDVIGLQETKVHDDMFPLQDLAHLGYQLHYHGQK
CEEEEEECCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHCCEEEEECCCC
GHYGVALLTRMAPLAVRKGFPGDDAEAQRRVIMADIPTELGTLTVINGYFPQGESRDHPI
CCHHHHHHHHHHHHHHHCCCCCCCHHHHCEEEEEECCCCCCEEEEEECCCCCCCCCCCCE
KFPAKSKFYADLQRYLQDSLTPQNPVLIMGDMNISPRDIDIGIGDESRRRWLRSGKCSFL
ECCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHCCCCCCC
PEEREWLDRLLAWGLVDTYRQANPQGDDHFSWFDYRSKGFDDNRGLRIDLLLASQPLAQR
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCEEEEEECCCHHHHH
CTDTGIDYEIRAMEKPSDHAPIWAEFALS
HHHCCCCEEEEEECCCCCCCCEEEEEECC
>Mature Secondary Structure
MKFISFNINGLRARPHQLAAIVEKHSPDVIGLQETKVHDDMFPLQDLAHLGYQLHYHGQK
CEEEEEECCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHCCEEEEECCCC
GHYGVALLTRMAPLAVRKGFPGDDAEAQRRVIMADIPTELGTLTVINGYFPQGESRDHPI
CCHHHHHHHHHHHHHHHCCCCCCCHHHHCEEEEEECCCCCCEEEEEECCCCCCCCCCCCE
KFPAKSKFYADLQRYLQDSLTPQNPVLIMGDMNISPRDIDIGIGDESRRRWLRSGKCSFL
ECCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHCCCCCCC
PEEREWLDRLLAWGLVDTYRQANPQGDDHFSWFDYRSKGFDDNRGLRIDLLLASQPLAQR
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCEEEEEECCCHHHHH
CTDTGIDYEIRAMEKPSDHAPIWAEFALS
HHHCCCCEEEEEECCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]