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The map label for this gene is ghrA [H]

Identifier: 238919479

GI number: 238919479

Start: 1536152

End: 1537093

Strand: Direct

Name: ghrA [H]

Synonym: NT01EI_1576

Alternate gene names: 238919479

Gene position: 1536152-1537093 (Clockwise)

Preceding gene: 238919478

Following gene: 238919481

Centisome position: 40.29

GC content: 62.42

Gene sequence:

>942_bases
ATGGAGATCATCTACTACCACCCTTTTTTTAATGCCCAGACCTGGCTTGACGGTATGCGCCAGCGTCTGCCGCAGGCCGA
TATCCGCCAGTGGCAGCCGGGCGATAACCGGTCCGCCGACTATGCGCTGGTCTGGCAGCCCCCCTATGAGATGCTGGCTC
AGCGTCAGGGGCTGCGTGCCATATTTGCCCTTGGCGCGGGCGTGGATGCCATTCTGTCGCAGGCGCGCGCGCATCCCGGG
ATGTTGCCGGCGGGCGTGCCGCTGGTGCGTCTGGAGGACACCGGAATGGCCCAGCAGATGGAGGAGTACGCCGTCGCGAC
GGTGATGCGCTATTTCCGCCGCTTTGATGAGTATGCGTTGCAGCAGCGTCAGGGGATCTGGCGTTATCTGACACCTCATG
AGGCTGGTACGTTTAGCGTGGGGGTGCTCGGCGCCGGTGTTCTGGGGGGAAGGGTGGCGCGTCGCCTGGCTTCCTTTGGC
ATGCCGGTGCGCTGCTGGAGTCGCACGGTGAAGGATTATCCGCAGGTGCAAAGCTTTTATGGCAGTGGGCAGCTGCCTGC
CTTTTTGCAGGGGTTGCAGCTGCTGATTAACCTACTGCCGAACACGCCACAGACCGTGGGCATACTGAATCAGTCCCTGT
TTGCCCGGATGAAGGCCGGCGCCTATATCATCAATCTGGCCCGCGGGGTACATCTGGATCAAGATGCGCTGCTGGCGGCG
CTGGATAATGGTCAGGTGGCGGCGGCAACGCTGGACGTATTCGATCAGGAGCCACTGGCAGCGGATCATCCGTTCTGGCA
GCATCCGCGGGTGACCATGACGCCGCATATCGCGGCGATCACCCTGCCGCAGGTGGCGATGGACTATATTGCCGATAATA
TCCATGCGATCGAGGCCGGGCGGCGCCCAGAAGGGGGGGTTGACGTCGCGCGTGGCTACTGA

Upstream 100 bases:

>100_bases
CGTTGACAGACGGCGGAGATCCTGACAAAAGGGTATTCGCCGTTTTTTTATCCGCGAGGCAAACGGTGGAGAGCGTCGTC
AACACGATCAGGAGGCCGAC

Downstream 100 bases:

>100_bases
TGAGGCGGGGGCGGCGCGCCCTCCGCCCGTCGGGGCGGCAGGGTAACGCAGTGTTTGCCCCGGTGCTTGCTGATATACAG
CCGATTGTCCGCCAGGGTCT

Product: 2-hydroxyacid dehydrogenase GhrA

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MEIIYYHPFFNAQTWLDGMRQRLPQADIRQWQPGDNRSADYALVWQPPYEMLAQRQGLRAIFALGAGVDAILSQARAHPG
MLPAGVPLVRLEDTGMAQQMEEYAVATVMRYFRRFDEYALQQRQGIWRYLTPHEAGTFSVGVLGAGVLGGRVARRLASFG
MPVRCWSRTVKDYPQVQSFYGSGQLPAFLQGLQLLINLLPNTPQTVGILNQSLFARMKAGAYIINLARGVHLDQDALLAA
LDNGQVAAATLDVFDQEPLAADHPFWQHPRVTMTPHIAAITLPQVAMDYIADNIHAIEAGRRPEGGVDVARGY

Sequences:

>Translated_313_residues
MEIIYYHPFFNAQTWLDGMRQRLPQADIRQWQPGDNRSADYALVWQPPYEMLAQRQGLRAIFALGAGVDAILSQARAHPG
MLPAGVPLVRLEDTGMAQQMEEYAVATVMRYFRRFDEYALQQRQGIWRYLTPHEAGTFSVGVLGAGVLGGRVARRLASFG
MPVRCWSRTVKDYPQVQSFYGSGQLPAFLQGLQLLINLLPNTPQTVGILNQSLFARMKAGAYIINLARGVHLDQDALLAA
LDNGQVAAATLDVFDQEPLAADHPFWQHPRVTMTPHIAAITLPQVAMDYIADNIHAIEAGRRPEGGVDVARGY
>Mature_313_residues
MEIIYYHPFFNAQTWLDGMRQRLPQADIRQWQPGDNRSADYALVWQPPYEMLAQRQGLRAIFALGAGVDAILSQARAHPG
MLPAGVPLVRLEDTGMAQQMEEYAVATVMRYFRRFDEYALQQRQGIWRYLTPHEAGTFSVGVLGAGVLGGRVARRLASFG
MPVRCWSRTVKDYPQVQSFYGSGQLPAFLQGLQLLINLLPNTPQTVGILNQSLFARMKAGAYIINLARGVHLDQDALLAA
LDNGQVAAATLDVFDQEPLAADHPFWQHPRVTMTPHIAAITLPQVAMDYIADNIHAIEAGRRPEGGVDVARGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=232, Percent_Identity=31.4655172413793, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI6912396, Length=230, Percent_Identity=27.8260869565217, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI145580578, Length=243, Percent_Identity=25.9259259259259, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI4557499, Length=243, Percent_Identity=25.9259259259259, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI145580575, Length=243, Percent_Identity=25.9259259259259, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI87081824, Length=313, Percent_Identity=57.8274760383387, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI87082289, Length=257, Percent_Identity=29.9610894941634, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789279, Length=197, Percent_Identity=26.9035532994924, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=307, Percent_Identity=26.7100977198697, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6325144, Length=148, Percent_Identity=31.7567567567568, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320925, Length=202, Percent_Identity=24.2574257425743, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322116, Length=202, Percent_Identity=23.2673267326733, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI28574286, Length=236, Percent_Identity=25.8474576271186, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI45552429, Length=231, Percent_Identity=25.1082251082251, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24585514, Length=231, Percent_Identity=25.1082251082251, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28574282, Length=231, Percent_Identity=25.1082251082251, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28574284, Length=231, Percent_Identity=25.1082251082251, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI45551003, Length=231, Percent_Identity=25.1082251082251, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI28571528, Length=199, Percent_Identity=31.1557788944724, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19921140, Length=164, Percent_Identity=28.6585365853659, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24585516, Length=256, Percent_Identity=25, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 34799; Mature: 34799

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIIYYHPFFNAQTWLDGMRQRLPQADIRQWQPGDNRSADYALVWQPPYEMLAQRQGLRA
CEEEEECCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHCCHHH
IFALGAGVDAILSQARAHPGMLPAGVPLVRLEDTGMAQQMEEYAVATVMRYFRRFDEYAL
HHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
QQRQGIWRYLTPHEAGTFSVGVLGAGVLGGRVARRLASFGMPVRCWSRTVKDYPQVQSFY
HHHCCCEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
GSGQLPAFLQGLQLLINLLPNTPQTVGILNQSLFARMKAGAYIINLARGVHLDQDALLAA
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHEEEHHHCCCCCHHHHHEE
LDNGQVAAATLDVFDQEPLAADHPFWQHPRVTMTPHIAAITLPQVAMDYIADNIHAIEAG
CCCCCEEEEEEHHHCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCHHEECCC
RRPEGGVDVARGY
CCCCCCCCCCCCC
>Mature Secondary Structure
MEIIYYHPFFNAQTWLDGMRQRLPQADIRQWQPGDNRSADYALVWQPPYEMLAQRQGLRA
CEEEEECCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHCCHHH
IFALGAGVDAILSQARAHPGMLPAGVPLVRLEDTGMAQQMEEYAVATVMRYFRRFDEYAL
HHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
QQRQGIWRYLTPHEAGTFSVGVLGAGVLGGRVARRLASFGMPVRCWSRTVKDYPQVQSFY
HHHCCCEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
GSGQLPAFLQGLQLLINLLPNTPQTVGILNQSLFARMKAGAYIINLARGVHLDQDALLAA
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHEEEHHHCCCCCHHHHHEE
LDNGQVAAATLDVFDQEPLAADHPFWQHPRVTMTPHIAAITLPQVAMDYIADNIHAIEAG
CCCCCEEEEEEHHHCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCHHEECCC
RRPEGGVDVARGY
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA