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The map label for this gene is insA [H]

Identifier: 238919455

GI number: 238919455

Start: 1513602

End: 1513874

Strand: Direct

Name: insA [H]

Synonym: NT01EI_1549

Alternate gene names: 238919455

Gene position: 1513602-1513874 (Clockwise)

Preceding gene: 238919452

Following gene: 238919456

Centisome position: 39.7

GC content: 52.01

Gene sequence:

>273_bases
ATGGTCAGCGTTAACGTTCATTGTCCCCGTTGTCAGTCTGCTCAGGTTTACCGCCATGGCCAGAATCCTAAAGGCCATGA
CAGATTTCGCTGCCGTGACGGTCGCCGCGTCTTTCAACTTACTTACGCCTATGCAGCCCGTAAACCGGGTATTAAAGAGC
AGATCACTGAAATGGCCTTCAAGGGGGCCGGGGTACGCGATACCGCCAGTACACTGAAAATTGGCATTAACACCGTCATC
CGGGCGTTAAAAAACGTGCGCCAAAGCGAATAA

Upstream 100 bases:

>100_bases
TGCATTCATCCAGCGCTTTATGGATTGCCGTCACCTTTTCATCGGTAATGCCTCTAATTAGTTGAATCTGATGTACAATG
TTCTCTTTTGAGGTTCTTTC

Downstream 100 bases:

>100_bases
CGTCATTCACCCGTCGCCCATACTGATGGAGCGCTTATCTGCGAACTTGATGAGCAATGGAGCTTCGTGGGCAGTAAAGC
CCGGGAGCGACTGGCACGGC

Product: insertion element iso-IS1N protein InsA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 90; Mature: 90

Protein sequence:

>90_residues
MVSVNVHCPRCQSAQVYRHGQNPKGHDRFRCRDGRRVFQLTYAYAARKPGIKEQITEMAFKGAGVRDTASTLKIGINTVI
RALKNVRQSE

Sequences:

>Translated_90_residues
MVSVNVHCPRCQSAQVYRHGQNPKGHDRFRCRDGRRVFQLTYAYAARKPGIKEQITEMAFKGAGVRDTASTLKIGINTVI
RALKNVRQSE
>Mature_90_residues
MVSVNVHCPRCQSAQVYRHGQNPKGHDRFRCRDGRRVFQLTYAYAARKPGIKEQITEMAFKGAGVRDTASTLKIGINTVI
RALKNVRQSE

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG3677

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS1 elements insA family [H]

Homologues:

Organism=Escherichia coli, GI1789852, Length=86, Percent_Identity=40.6976744186046, Blast_Score=82, Evalue=1e-17,
Organism=Escherichia coli, GI1788204, Length=86, Percent_Identity=40.6976744186046, Blast_Score=82, Evalue=1e-17,
Organism=Escherichia coli, GI1786204, Length=86, Percent_Identity=40.6976744186046, Blast_Score=82, Evalue=1e-17,
Organism=Escherichia coli, GI87081814, Length=86, Percent_Identity=40.6976744186046, Blast_Score=80, Evalue=2e-17,
Organism=Escherichia coli, GI1786471, Length=86, Percent_Identity=40.6976744186046, Blast_Score=80, Evalue=2e-17,
Organism=Escherichia coli, GI1786460, Length=86, Percent_Identity=40.6976744186046, Blast_Score=80, Evalue=2e-17,
Organism=Escherichia coli, GI1790747, Length=86, Percent_Identity=39.5348837209302, Blast_Score=80, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003220 [H]

Pfam domain/function: PF03811 Ins_element1 [H]

EC number: NA

Molecular weight: Translated: 10189; Mature: 10189

Theoretical pI: Translated: 11.07; Mature: 11.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSVNVHCPRCQSAQVYRHGQNPKGHDRFRCRDGRRVFQLTYAYAARKPGIKEQITEMAF
CEECCCCCCCCCCCHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHH
KGAGVRDTASTLKIGINTVIRALKNVRQSE
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVSVNVHCPRCQSAQVYRHGQNPKGHDRFRCRDGRRVFQLTYAYAARKPGIKEQITEMAF
CEECCCCCCCCCCCHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHH
KGAGVRDTASTLKIGINTVIRALKNVRQSE
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]