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The map label for this gene is gltX [H]

Identifier: 238919180

GI number: 238919180

Start: 1244244

End: 1245659

Strand: Direct

Name: gltX [H]

Synonym: NT01EI_1258

Alternate gene names: 238919180

Gene position: 1244244-1245659 (Clockwise)

Preceding gene: 238919179

Following gene: 238919184

Centisome position: 32.64

GC content: 58.33

Gene sequence:

>1416_bases
ATGAAAATCAAAACCCGTTTTGCGCCCAGCCCGACCGGCTATCTGCACGTCGGTGGCGCCCGTACTGCGCTTTACTCCTG
GCTGTATGCCCGCCATAACCAGGGCGAGTTTGTCCTGCGTATTGAAGATACCGATCTGGAGCGCTCCACGCAGGAAGCTA
TCGATGCCATTATGGATGGCATGAACTGGCTGAATCTGGATTGGGATGAGGGTCCGTACTACCAGACCAAGCGTTTCGAT
CGCTACAATCAGGTGATCGATCAGATGTTGGAGCAGGGGACCGCCTATAAATGTTATTGTTCCAAAGAGCACCTGGAGCA
GCTGCGCGAAGATCAGATGGCCCGAGGAGAGAAGCCGCGCTATGACGGTCGCTGCCGCCATGACCACTGCCACCATGCCG
ATGATGAGCCGCACGTGGTGCGTTTCCTCAACCCGCAGGAAGGCTCCGTCATTTTTGATGACAAGATCCGTGGCCCGATC
GAATTCAGTAACAAGGAGCTGGACGATCTGATCATCCGCCGTACCGATGGCTCACCGACCTATAACTTCTGTGTGGTGAT
CGATGACTGGGATATGGGGATCACCCACGTTATCCGCGGTGAGGATCACATCAACAATACGCCACGCCAGATCAACATCC
TGAAGGCGCTCGGCGCGCCGGTGCCGGAGTATGCCCACGTTTCGATGATCCTGGGCGATGATGGCAAGAAGCTGTCCAAA
CGCCACGGCGCCGTCGGCGTCATGCAGTACCGTGATGATGGCTATCTGCCGGAAGCGCTGCTGAACTACCTGGTGCGTCT
GGGCTGGGCCCACGGCGATCAGGAGATCTTTAGCGTGGCGGAAATGACGGAGTTCTTCACCCTGGACGCCATCAGCAAGT
CAGCCAGCGCCTTTAATACCGAAAAGTTGCAGTGGCTGAACCACCACTATATTAACCATCTGCCGCCGGAGCAGGTCGCC
GGACACCTGGCATGGCACGTGGCGCAGGCTGGCTATGATATCGCCAACGGTCCGCAGCTGACCGAGATCGTGACCCTGCT
GGGTGAGCGCTGTAAGACACTGAAAGAGATGGCGCAGCAGGCTCGCTATTTCTACGAGGAGTTTAGCGCGTTTGATGCCG
ACGCCGCGAAGAAGCACCTGCGTCCGGTCGCACGTCAGCCGTTGGAGCTGGCTCGCGCCAAACTGGCCGCTATCGGTGAC
TGGCAGGTGGCGTCTATCCACCATGCGATTCAGTCAACGGCCGATGAGCTGGAGGTCGGGATGGGCAAGGTTGGGATGCC
GCTGCGTGTCGCGGTGACCGGTCAGGGGCAATCGCCGAGCGTTGACGTGACTATCCACGCCATCGGTCAGCAGCGTGCGT
TGACGCGTATTGATCGCGCGTTGGCGTTTATCGATGAGCGTTCGGCGCAGCAGTAA

Upstream 100 bases:

>100_bases
GAATTGGGGGGTAATGGTAAAATGCCGCCCACAGATATTTTGCAACCCGCTTACCTATACTGGGAATGGTGGGTAACCTG
CGTAACAAGGCCACGTTCAC

Downstream 100 bases:

>100_bases
GCGTTGAGGACATAATAGATAGTCAAACCGGCTCTGGCCGGTTTTTTCTTGCCCGTCGCCGGCACAGGTTCAGTCACCCA
GACGGCGTAGAAACTCGTTG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLYARHNQGEFVLRIEDTDLERSTQEAIDAIMDGMNWLNLDWDEGPYYQTKRFD
RYNQVIDQMLEQGTAYKCYCSKEHLEQLREDQMARGEKPRYDGRCRHDHCHHADDEPHVVRFLNPQEGSVIFDDKIRGPI
EFSNKELDDLIIRRTDGSPTYNFCVVIDDWDMGITHVIRGEDHINNTPRQINILKALGAPVPEYAHVSMILGDDGKKLSK
RHGAVGVMQYRDDGYLPEALLNYLVRLGWAHGDQEIFSVAEMTEFFTLDAISKSASAFNTEKLQWLNHHYINHLPPEQVA
GHLAWHVAQAGYDIANGPQLTEIVTLLGERCKTLKEMAQQARYFYEEFSAFDADAAKKHLRPVARQPLELARAKLAAIGD
WQVASIHHAIQSTADELEVGMGKVGMPLRVAVTGQGQSPSVDVTIHAIGQQRALTRIDRALAFIDERSAQQ

Sequences:

>Translated_471_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLYARHNQGEFVLRIEDTDLERSTQEAIDAIMDGMNWLNLDWDEGPYYQTKRFD
RYNQVIDQMLEQGTAYKCYCSKEHLEQLREDQMARGEKPRYDGRCRHDHCHHADDEPHVVRFLNPQEGSVIFDDKIRGPI
EFSNKELDDLIIRRTDGSPTYNFCVVIDDWDMGITHVIRGEDHINNTPRQINILKALGAPVPEYAHVSMILGDDGKKLSK
RHGAVGVMQYRDDGYLPEALLNYLVRLGWAHGDQEIFSVAEMTEFFTLDAISKSASAFNTEKLQWLNHHYINHLPPEQVA
GHLAWHVAQAGYDIANGPQLTEIVTLLGERCKTLKEMAQQARYFYEEFSAFDADAAKKHLRPVARQPLELARAKLAAIGD
WQVASIHHAIQSTADELEVGMGKVGMPLRVAVTGQGQSPSVDVTIHAIGQQRALTRIDRALAFIDERSAQQ
>Mature_471_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLYARHNQGEFVLRIEDTDLERSTQEAIDAIMDGMNWLNLDWDEGPYYQTKRFD
RYNQVIDQMLEQGTAYKCYCSKEHLEQLREDQMARGEKPRYDGRCRHDHCHHADDEPHVVRFLNPQEGSVIFDDKIRGPI
EFSNKELDDLIIRRTDGSPTYNFCVVIDDWDMGITHVIRGEDHINNTPRQINILKALGAPVPEYAHVSMILGDDGKKLSK
RHGAVGVMQYRDDGYLPEALLNYLVRLGWAHGDQEIFSVAEMTEFFTLDAISKSASAFNTEKLQWLNHHYINHLPPEQVA
GHLAWHVAQAGYDIANGPQLTEIVTLLGERCKTLKEMAQQARYFYEEFSAFDADAAKKHLRPVARQPLELARAKLAAIGD
WQVASIHHAIQSTADELEVGMGKVGMPLRVAVTGQGQSPSVDVTIHAIGQQRALTRIDRALAFIDERSAQQ

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=494, Percent_Identity=30.9716599190283, Blast_Score=230, Evalue=3e-60,
Organism=Escherichia coli, GI1788743, Length=471, Percent_Identity=82.3779193205945, Blast_Score=830, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=240, Percent_Identity=37.0833333333333, Blast_Score=148, Evalue=8e-37,
Organism=Escherichia coli, GI1786895, Length=122, Percent_Identity=27.0491803278689, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=475, Percent_Identity=30.1052631578947, Blast_Score=207, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6324540, Length=502, Percent_Identity=31.8725099601594, Blast_Score=243, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6321192, Length=230, Percent_Identity=27.8260869565217, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324742, Length=213, Percent_Identity=26.2910798122066, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24665321, Length=492, Percent_Identity=30.8943089430894, Blast_Score=243, Evalue=3e-64,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 53491; Mature: 53491

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKTRFAPSPTGYLHVGGARTALYSWLYARHNQGEFVLRIEDTDLERSTQEAIDAIMDG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCC
MNWLNLDWDEGPYYQTKRFDRYNQVIDQMLEQGTAYKCYCSKEHLEQLREDQMARGEKPR
CCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCCCC
YDGRCRHDHCHHADDEPHVVRFLNPQEGSVIFDDKIRGPIEFSNKELDDLIIRRTDGSPT
CCCCCCCCCCCCCCCCCCEEEEECCCCCCEEECCCCCCCCCCCCCCCCHHEEEECCCCCC
YNFCVVIDDWDMGITHVIRGEDHINNTPRQINILKALGAPVPEYAHVSMILGDDGKKLSK
EEEEEEEECCCCCCEEEECCCHHCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHH
RHGAVGVMQYRDDGYLPEALLNYLVRLGWAHGDQEIFSVAEMTEFFTLDAISKSASAFNT
HCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCH
EKLQWLNHHYINHLPPEQVAGHLAWHVAQAGYDIANGPQLTEIVTLLGERCKTLKEMAQQ
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ARYFYEEFSAFDADAAKKHLRPVARQPLELARAKLAAIGDWQVASIHHAIQSTADELEVG
HHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
MGKVGMPLRVAVTGQGQSPSVDVTIHAIGQQRALTRIDRALAFIDERSAQQ
CCCCCCCEEEEEECCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIKTRFAPSPTGYLHVGGARTALYSWLYARHNQGEFVLRIEDTDLERSTQEAIDAIMDG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCC
MNWLNLDWDEGPYYQTKRFDRYNQVIDQMLEQGTAYKCYCSKEHLEQLREDQMARGEKPR
CCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCCCC
YDGRCRHDHCHHADDEPHVVRFLNPQEGSVIFDDKIRGPIEFSNKELDDLIIRRTDGSPT
CCCCCCCCCCCCCCCCCCEEEEECCCCCCEEECCCCCCCCCCCCCCCCHHEEEECCCCCC
YNFCVVIDDWDMGITHVIRGEDHINNTPRQINILKALGAPVPEYAHVSMILGDDGKKLSK
EEEEEEEECCCCCCEEEECCCHHCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHH
RHGAVGVMQYRDDGYLPEALLNYLVRLGWAHGDQEIFSVAEMTEFFTLDAISKSASAFNT
HCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCH
EKLQWLNHHYINHLPPEQVAGHLAWHVAQAGYDIANGPQLTEIVTLLGERCKTLKEMAQQ
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ARYFYEEFSAFDADAAKKHLRPVARQPLELARAKLAAIGDWQVASIHHAIQSTADELEVG
HHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
MGKVGMPLRVAVTGQGQSPSVDVTIHAIGQQRALTRIDRALAFIDERSAQQ
CCCCCCCEEEEEECCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA