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The map label for this gene is ligA [H]

Identifier: 238919178

GI number: 238919178

Start: 1240104

End: 1242122

Strand: Direct

Name: ligA [H]

Synonym: NT01EI_1249

Alternate gene names: 238919178

Gene position: 1240104-1242122 (Clockwise)

Preceding gene: 238919177

Following gene: 238919179

Centisome position: 32.53

GC content: 62.75

Gene sequence:

>2019_bases
ATGATTGAACAACAACTCGAACAGCTGCGTGATGCGCTGCGCCATCATGAGTATCAATACTACGTTTTGGACGATCCGCA
GATTACCGATGCAGAGTACGATCGCCTGATGATGCAGCTTAAGCAGCTGGAGGCCGTGCACCCGGCGCTGGTGACGGTGG
ATTCCCCGAGCCAGCGCGTCGGTGGCGCACCGCTGGCAGCCTTTGGCCAGGTGCACCATGAGGTGCCGATGCTCTCGTTG
GATAACGTATTTGATGAGGAAAGTTATCAGGCGTTTGATAAGCGCCTGCATGAGCGGCTGAAAAGTGACGAACCGCTGAC
CTTCTGCTGTGAGCCAAAGCTGGACGGATTGGCGGTAAGCCTGCTGTATGAGCAGGGGGTACTGGTACGCGCGGCGACGC
GCGGTGATGGGACAACCGGCGAAGATATTACCAGTAATGTGAAAACCATTCGCGCCATCCCACTGCGTTTGCAGGGAGAG
GCTATCCCTGCACGCATTGAGGTTCGTGGCGAGGTCTTCATGCCGCAGGATGGCTTTGAGGCGATGAATGAGGAGGCGCG
CCGGCGCGGCGCGAAAATTTTTGCCAATCCGCGCAACGCCGCTGCCGGATCGCTGCGACAGCTTGACCCGCGCATCACCG
CGCAGCGCCCGCTGACCTTCTACTGCTACGGCACAGGCCAGCTGGAGGGGGGCGCGCTGCCGGACAGTCACTATCAGTGC
CTGCAGCGCTTTAAGTCCTGGGGACTGCCGGTCAGCGAACGGGTGAGGCTGTGCTGCGGTAGCGCAGAGGTGCTGGCCTA
CTATCGCCAGATCGGGCAGGCGCGACCGACGTTAGGCTTTGATATCGACGGCGTGGTGATCAAGGTGGACGATATCGCCA
TCCAGGAGCGGCTGGGCTTTGTCGCCCGCGCGCCGCGCTGGGCGACGGCGTTCAAGTTTCCGGCACAGGAGCAGATGACG
CGGCTGAACGGGGTTGAGTTTCAGGTCGGTCGCACCGGGGCCATCACGCCGGTCGCGCGCCTGGAGCCGGTGCTGGTGGC
CGGGGTGACCGTCAGCAACGCGACGCTGCACAATGCGGATGAAATTGAGCGTCTGGATCTGCGGATCGGCGATACGGTGA
TCGTCCGCCGCGCCGGTGACGTGATCCCGCAGGTGGTTGGGGTGGTGGAGGCGATGCGTCCGCGCGATGCCCACGAGATC
GGCTTTCCGACCCACTGTCCGGTATGTGGCTCTGAGGTGGAGCGGACGGAGGGCGAGGCGGTGGCACGCTGCAGCGGTGG
CCTGATCTGTGCCGCGCAGCGCAAGGAGTCGCTGAAGCATTTTGTATCGCGCCGCGCGATGGATGTCGAGGGGATGGGCG
ATAAGGTCATTGAACAGCTGGTGGATAAAGAGTATGTCGCTACGCCCGCCGATCTGTACCGCCTGAGCGCCGGTAAGCTG
ACCGGCCTGGAGCGGATGGGGCCCAAGTCGGCCACCAAGCTTATTGCTGCCCTCGATCGCTCGCGGGAAACCACGCTGGC
CCGTTTTCTCTTTGCGCTGGGCATCCGCGAGGTGGGGGAGGCGACGGCGGCCAATCTGGCCAGCCACTTTGGTTCACTGC
AGGCGCTGCGCGCGGCGGATCTGGAGAGCCTCAAGCGCGTTCCCGAGGTGGGTGAGGTGGTTGCCAGCCATATCGTCCAC
TTTTTGAGCGAGGAACATAACCGCCAGGTGATCGATGAGCTGATCTCCGACGAAGTTGGCATCCGCTGGCCGGAGGGGGC
ACCGGCGCAGTCTGCGCCCGCAGTCGACAGCCCGTTTGCCGGAAAGACCGTGGTATTGACCGGCTCACTGAGCGTACTGT
CGCGCGATGAGGCGAAGGATCGTTTGACAGCATTGGGCGCCCGGGTCAGCGGCAGCGTATCGAAGAAGACCGATCTGGTG
ATCGCCGGCGATGCGGCGGGCTCCAAGTTGGCGAAGGCCAATATTCTGGGCATCGAGGTGATCGATGAGGCGCAGATGCT
GCGCATGCTGGGGGCATAA

Upstream 100 bases:

>100_bases
AGAACTTGGGCCGGACCGCGTGACGTGATCGTGCCAGCGTCTTACCAAAACCCCCGCCCGTCGGGGGTTTTTTATCATTA
AATGGTGGCTTATGACTCCC

Downstream 100 bases:

>100_bases
TGGCTCCCGTCACCAACCATAGGGCACCGCGCGCTGTTTCTGTGAGCAGACAGAGGGCTGCGTTGATGGGATAGGTTAAA
GTTGACGTGCATTTGCGCCG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 672; Mature: 672

Protein sequence:

>672_residues
MIEQQLEQLRDALRHHEYQYYVLDDPQITDAEYDRLMMQLKQLEAVHPALVTVDSPSQRVGGAPLAAFGQVHHEVPMLSL
DNVFDEESYQAFDKRLHERLKSDEPLTFCCEPKLDGLAVSLLYEQGVLVRAATRGDGTTGEDITSNVKTIRAIPLRLQGE
AIPARIEVRGEVFMPQDGFEAMNEEARRRGAKIFANPRNAAAGSLRQLDPRITAQRPLTFYCYGTGQLEGGALPDSHYQC
LQRFKSWGLPVSERVRLCCGSAEVLAYYRQIGQARPTLGFDIDGVVIKVDDIAIQERLGFVARAPRWATAFKFPAQEQMT
RLNGVEFQVGRTGAITPVARLEPVLVAGVTVSNATLHNADEIERLDLRIGDTVIVRRAGDVIPQVVGVVEAMRPRDAHEI
GFPTHCPVCGSEVERTEGEAVARCSGGLICAAQRKESLKHFVSRRAMDVEGMGDKVIEQLVDKEYVATPADLYRLSAGKL
TGLERMGPKSATKLIAALDRSRETTLARFLFALGIREVGEATAANLASHFGSLQALRAADLESLKRVPEVGEVVASHIVH
FLSEEHNRQVIDELISDEVGIRWPEGAPAQSAPAVDSPFAGKTVVLTGSLSVLSRDEAKDRLTALGARVSGSVSKKTDLV
IAGDAAGSKLAKANILGIEVIDEAQMLRMLGA

Sequences:

>Translated_672_residues
MIEQQLEQLRDALRHHEYQYYVLDDPQITDAEYDRLMMQLKQLEAVHPALVTVDSPSQRVGGAPLAAFGQVHHEVPMLSL
DNVFDEESYQAFDKRLHERLKSDEPLTFCCEPKLDGLAVSLLYEQGVLVRAATRGDGTTGEDITSNVKTIRAIPLRLQGE
AIPARIEVRGEVFMPQDGFEAMNEEARRRGAKIFANPRNAAAGSLRQLDPRITAQRPLTFYCYGTGQLEGGALPDSHYQC
LQRFKSWGLPVSERVRLCCGSAEVLAYYRQIGQARPTLGFDIDGVVIKVDDIAIQERLGFVARAPRWATAFKFPAQEQMT
RLNGVEFQVGRTGAITPVARLEPVLVAGVTVSNATLHNADEIERLDLRIGDTVIVRRAGDVIPQVVGVVEAMRPRDAHEI
GFPTHCPVCGSEVERTEGEAVARCSGGLICAAQRKESLKHFVSRRAMDVEGMGDKVIEQLVDKEYVATPADLYRLSAGKL
TGLERMGPKSATKLIAALDRSRETTLARFLFALGIREVGEATAANLASHFGSLQALRAADLESLKRVPEVGEVVASHIVH
FLSEEHNRQVIDELISDEVGIRWPEGAPAQSAPAVDSPFAGKTVVLTGSLSVLSRDEAKDRLTALGARVSGSVSKKTDLV
IAGDAAGSKLAKANILGIEVIDEAQMLRMLGA
>Mature_672_residues
MIEQQLEQLRDALRHHEYQYYVLDDPQITDAEYDRLMMQLKQLEAVHPALVTVDSPSQRVGGAPLAAFGQVHHEVPMLSL
DNVFDEESYQAFDKRLHERLKSDEPLTFCCEPKLDGLAVSLLYEQGVLVRAATRGDGTTGEDITSNVKTIRAIPLRLQGE
AIPARIEVRGEVFMPQDGFEAMNEEARRRGAKIFANPRNAAAGSLRQLDPRITAQRPLTFYCYGTGQLEGGALPDSHYQC
LQRFKSWGLPVSERVRLCCGSAEVLAYYRQIGQARPTLGFDIDGVVIKVDDIAIQERLGFVARAPRWATAFKFPAQEQMT
RLNGVEFQVGRTGAITPVARLEPVLVAGVTVSNATLHNADEIERLDLRIGDTVIVRRAGDVIPQVVGVVEAMRPRDAHEI
GFPTHCPVCGSEVERTEGEAVARCSGGLICAAQRKESLKHFVSRRAMDVEGMGDKVIEQLVDKEYVATPADLYRLSAGKL
TGLERMGPKSATKLIAALDRSRETTLARFLFALGIREVGEATAANLASHFGSLQALRAADLESLKRVPEVGEVVASHIVH
FLSEEHNRQVIDELISDEVGIRWPEGAPAQSAPAVDSPFAGKTVVLTGSLSVLSRDEAKDRLTALGARVSGSVSKKTDLV
IAGDAAGSKLAKANILGIEVIDEAQMLRMLGA

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=672, Percent_Identity=73.8095238095238, Blast_Score=1021, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=521, Percent_Identity=22.4568138195777, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 73408; Mature: 73408

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEQQLEQLRDALRHHEYQYYVLDDPQITDAEYDRLMMQLKQLEAVHPALVTVDSPSQRV
CCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCHHHC
GGAPLAAFGQVHHEVPMLSLDNVFDEESYQAFDKRLHERLKSDEPLTFCCEPKLDGLAVS
CCCCHHHHHHHHHCCCCEEHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEE
LLYEQGVLVRAATRGDGTTGEDITSNVKTIRAIPLRLQGEAIPARIEVRGEVFMPQDGFE
EEHHCCEEEEEECCCCCCCCHHHHHCCCEEEEECEEEECCCCCEEEEEECEEECCCCCHH
AMNEEARRRGAKIFANPRNAAAGSLRQLDPRITAQRPLTFYCYGTGQLEGGALPDSHYQC
HHHHHHHHCCCEEEECCCCCHHCCHHHCCCCCCCCCCEEEEEEECCEECCCCCCHHHHHH
LQRFKSWGLPVSERVRLCCGSAEVLAYYRQIGQARPTLGFDIDGVVIKVDDIAIQERLGF
HHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHH
VARAPRWATAFKFPAQEQMTRLNGVEFQVGRTGAITPVARLEPVLVAGVTVSNATLHNAD
HHCCCCHHHHCCCCCHHHHHHHCCCEEEECCCCCCCHHHHCCCEEEEEEEECCCCCCCHH
EIERLDLRIGDTVIVRRAGDVIPQVVGVVEAMRPRDAHEIGFPTHCPVCGSEVERTEGEA
HHHHHHEEECCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCH
VARCSGGLICAAQRKESLKHFVSRRAMDVEGMGDKVIEQLVDKEYVATPADLYRLSAGKL
HHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCHHHCCCCHHHHHCCCCCC
TGLERMGPKSATKLIAALDRSRETTLARFLFALGIREVGEATAANLASHFGSLQALRAAD
CCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LESLKRVPEVGEVVASHIVHFLSEEHNRQVIDELISDEVGIRWPEGAPAQSAPAVDSPFA
HHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
GKTVVLTGSLSVLSRDEAKDRLTALGARVSGSVSKKTDLVIAGDAAGSKLAKANILGIEV
CCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHCEEEEEEE
IDEAQMLRMLGA
HHHHHHHHHHCC
>Mature Secondary Structure
MIEQQLEQLRDALRHHEYQYYVLDDPQITDAEYDRLMMQLKQLEAVHPALVTVDSPSQRV
CCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCHHHC
GGAPLAAFGQVHHEVPMLSLDNVFDEESYQAFDKRLHERLKSDEPLTFCCEPKLDGLAVS
CCCCHHHHHHHHHCCCCEEHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEE
LLYEQGVLVRAATRGDGTTGEDITSNVKTIRAIPLRLQGEAIPARIEVRGEVFMPQDGFE
EEHHCCEEEEEECCCCCCCCHHHHHCCCEEEEECEEEECCCCCEEEEEECEEECCCCCHH
AMNEEARRRGAKIFANPRNAAAGSLRQLDPRITAQRPLTFYCYGTGQLEGGALPDSHYQC
HHHHHHHHCCCEEEECCCCCHHCCHHHCCCCCCCCCCEEEEEEECCEECCCCCCHHHHHH
LQRFKSWGLPVSERVRLCCGSAEVLAYYRQIGQARPTLGFDIDGVVIKVDDIAIQERLGF
HHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHH
VARAPRWATAFKFPAQEQMTRLNGVEFQVGRTGAITPVARLEPVLVAGVTVSNATLHNAD
HHCCCCHHHHCCCCCHHHHHHHCCCEEEECCCCCCCHHHHCCCEEEEEEEECCCCCCCHH
EIERLDLRIGDTVIVRRAGDVIPQVVGVVEAMRPRDAHEIGFPTHCPVCGSEVERTEGEA
HHHHHHEEECCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCH
VARCSGGLICAAQRKESLKHFVSRRAMDVEGMGDKVIEQLVDKEYVATPADLYRLSAGKL
HHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCHHHCCCCHHHHHCCCCCC
TGLERMGPKSATKLIAALDRSRETTLARFLFALGIREVGEATAANLASHFGSLQALRAAD
CCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LESLKRVPEVGEVVASHIVHFLSEEHNRQVIDELISDEVGIRWPEGAPAQSAPAVDSPFA
HHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
GKTVVLTGSLSVLSRDEAKDRLTALGARVSGSVSKKTDLVIAGDAAGSKLAKANILGIEV
CCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHCEEEEEEE
IDEAQMLRMLGA
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA