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The map label for this gene is amtB [H]

Identifier: 238919024

GI number: 238919024

Start: 1086848

End: 1088125

Strand: Direct

Name: amtB [H]

Synonym: NT01EI_1091

Alternate gene names: 238919024

Gene position: 1086848-1088125 (Clockwise)

Preceding gene: 238919023

Following gene: 238919027

Centisome position: 28.51

GC content: 64.08

Gene sequence:

>1278_bases
ATGCGATTGGGCAGGGGGATGGTGAGCGTGGCGCTGCTGCCGGGGGTTGCGCTGGCCGGCGCGCCACAGGTGGATCGGGC
GGATAACGGCTTTATGATGCTAGCGACGGCGCTGGTGCTGTTGATGACCCTGCCGGGGATCGCGCTGTTTTACGGCGGCC
TGCTGCGCGCTAAAAACGTGCTGTCGCTGATGACACAGGTGATGGTGGCCTTCGCCGTCGTGAGCGTGCTGTGGGTGATC
TATGGCTATACGCTGGCATTTGGCCGTGGGAACGCCGTCGTCGGTAATCTGCACGCTCTGTTGCTCAGGGGGATCGGATT
GCACGAGGTGACCGGCTCGATATCGACGCTAGTGCACGTGGCCTTTCAGGGATCGTTTGCCTGTATTACGGTGGCGCTGG
TGACCGGCGCACTGGCTGAGCGGGCCCGTTTTTCGGCGGTGCTGATCTTTGCCGCGCTGTGGTTTTCGCTCTCCTATGTG
CCGCTGGCCCATATGGTCTGGGGCGGGGGCTGGCTGGCCGCCGACGGTGCGCTGGACTTTGCCGGTGGGACGGTAGTGCA
TATTAATGCCGCCGTGGCAGGACTGCTGGGCGCCCGCCTGCTGGGACGGCGCAGCGGCTATGGGCGGGAGTCGTTTAAGC
CGCACAGTGTACCGATGGTTTTTCTTGGCACCTCAATTCTGTATATCGGCTGGTTTGGCTTTAACGCGGGCTCCGCCGGT
GCTGCCAACGATATTGCTGCGCTGGCGTTTATCAACACGATGATCGCCACCGCCGCCGCCATTCTCTCTTGGGTCTTGGC
GGAATGGGGGCTACGCGGGAATCCTTCCCTGCTGGGAGCCTGCTCCGGCTGTATTGCCGGGCTGGTGGCAGTGACGCCGG
CGGCGGGCAGCGTGGGTGTCGGTGGCGCGCTGCTGCTTGGCCTGATCGCGGGTGCGGCAGGGCTATGGGGCGTGGTGGTA
TTGAAACGCTGGCTGCGGACAGATGACGCCTGTGATGTGTTCGGCGTACACGGCGTCTGCGGTATCGTCGGGTGTCTGGC
TACCGGGGTACTGAGTGCTGCCCCGCTGGGCGGTACGGGCTATGCCAGCGGTGTCACCATGGGTCACCAGCTGTGGGTTC
AGATGCTGAGTGTGCTGGTCTGCGTGATCTGGTCCGCGGCGGCGGCGCTGATTGCCTATAAGGCGGCGGATCTCTGCGTG
GGGCTGCGGGTCAGTGAGGATCAGGAGCGCGAGGGGCTGGATGTGACCAGCCACGGTGAGAACGCCTATAACCGCTAG

Upstream 100 bases:

>100_bases
CGGTAAGATTTTTGTTGCCGAACTGACGCGCGTGGTGCGCATTCGCACCGGTGAGTGCGATGACGCGGCGCTGTAATGGC
CCGCATGGAGGTGTGAGATG

Downstream 100 bases:

>100_bases
ACGCGCGGCGCCGTGCTGCGCGCTGCAGGGCGCTTAGCACGCCGGATGCTGCCGAATAACCCCCTCCTGCGCGGCGGAGG
CGACCAGACGTCCCTGGCGG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MRLGRGMVSVALLPGVALAGAPQVDRADNGFMMLATALVLLMTLPGIALFYGGLLRAKNVLSLMTQVMVAFAVVSVLWVI
YGYTLAFGRGNAVVGNLHALLLRGIGLHEVTGSISTLVHVAFQGSFACITVALVTGALAERARFSAVLIFAALWFSLSYV
PLAHMVWGGGWLAADGALDFAGGTVVHINAAVAGLLGARLLGRRSGYGRESFKPHSVPMVFLGTSILYIGWFGFNAGSAG
AANDIAALAFINTMIATAAAILSWVLAEWGLRGNPSLLGACSGCIAGLVAVTPAAGSVGVGGALLLGLIAGAAGLWGVVV
LKRWLRTDDACDVFGVHGVCGIVGCLATGVLSAAPLGGTGYASGVTMGHQLWVQMLSVLVCVIWSAAAALIAYKAADLCV
GLRVSEDQEREGLDVTSHGENAYNR

Sequences:

>Translated_425_residues
MRLGRGMVSVALLPGVALAGAPQVDRADNGFMMLATALVLLMTLPGIALFYGGLLRAKNVLSLMTQVMVAFAVVSVLWVI
YGYTLAFGRGNAVVGNLHALLLRGIGLHEVTGSISTLVHVAFQGSFACITVALVTGALAERARFSAVLIFAALWFSLSYV
PLAHMVWGGGWLAADGALDFAGGTVVHINAAVAGLLGARLLGRRSGYGRESFKPHSVPMVFLGTSILYIGWFGFNAGSAG
AANDIAALAFINTMIATAAAILSWVLAEWGLRGNPSLLGACSGCIAGLVAVTPAAGSVGVGGALLLGLIAGAAGLWGVVV
LKRWLRTDDACDVFGVHGVCGIVGCLATGVLSAAPLGGTGYASGVTMGHQLWVQMLSVLVCVIWSAAAALIAYKAADLCV
GLRVSEDQEREGLDVTSHGENAYNR
>Mature_425_residues
MRLGRGMVSVALLPGVALAGAPQVDRADNGFMMLATALVLLMTLPGIALFYGGLLRAKNVLSLMTQVMVAFAVVSVLWVI
YGYTLAFGRGNAVVGNLHALLLRGIGLHEVTGSISTLVHVAFQGSFACITVALVTGALAERARFSAVLIFAALWFSLSYV
PLAHMVWGGGWLAADGALDFAGGTVVHINAAVAGLLGARLLGRRSGYGRESFKPHSVPMVFLGTSILYIGWFGFNAGSAG
AANDIAALAFINTMIATAAAILSWVLAEWGLRGNPSLLGACSGCIAGLVAVTPAAGSVGVGGALLLGLIAGAAGLWGVVV
LKRWLRTDDACDVFGVHGVCGIVGCLATGVLSAAPLGGTGYASGVTMGHQLWVQMLSVLVCVIWSAAAALIAYKAADLCV
GLRVSEDQEREGLDVTSHGENAYNR

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=419, Percent_Identity=69.2124105011933, Blast_Score=602, Evalue=1e-173,
Organism=Caenorhabditis elegans, GI17550182, Length=439, Percent_Identity=29.1571753986333, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17550180, Length=433, Percent_Identity=28.175519630485, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI32564042, Length=425, Percent_Identity=26.1176470588235, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI133931100, Length=444, Percent_Identity=24.3243243243243, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324187, Length=423, Percent_Identity=37.1158392434988, Blast_Score=224, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6325396, Length=416, Percent_Identity=34.8557692307692, Blast_Score=219, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6321559, Length=407, Percent_Identity=33.4152334152334, Blast_Score=210, Evalue=3e-55,
Organism=Drosophila melanogaster, GI161078314, Length=405, Percent_Identity=27.6543209876543, Blast_Score=114, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 43597; Mature: 43597

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLGRGMVSVALLPGVALAGAPQVDRADNGFMMLATALVLLMTLPGIALFYGGLLRAKNV
CCCCCHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSLMTQVMVAFAVVSVLWVIYGYTLAFGRGNAVVGNLHALLLRGIGLHEVTGSISTLVHV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
AFQGSFACITVALVTGALAERARFSAVLIFAALWFSLSYVPLAHMVWGGGWLAADGALDF
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEC
AGGTVVHINAAVAGLLGARLLGRRSGYGRESFKPHSVPMVFLGTSILYIGWFGFNAGSAG
CCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCHHHHHEEECCCCCCCC
AANDIAALAFINTMIATAAAILSWVLAEWGLRGNPSLLGACSGCIAGLVAVTPAAGSVGV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
GGALLLGLIAGAAGLWGVVVLKRWLRTDDACDVFGVHGVCGIVGCLATGVLSAAPLGGTG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
YASGVTMGHQLWVQMLSVLVCVIWSAAAALIAYKAADLCVGLRVSEDQEREGLDVTSHGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCC
NAYNR
CCCCC
>Mature Secondary Structure
MRLGRGMVSVALLPGVALAGAPQVDRADNGFMMLATALVLLMTLPGIALFYGGLLRAKNV
CCCCCHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSLMTQVMVAFAVVSVLWVIYGYTLAFGRGNAVVGNLHALLLRGIGLHEVTGSISTLVHV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
AFQGSFACITVALVTGALAERARFSAVLIFAALWFSLSYVPLAHMVWGGGWLAADGALDF
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEC
AGGTVVHINAAVAGLLGARLLGRRSGYGRESFKPHSVPMVFLGTSILYIGWFGFNAGSAG
CCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCHHHHHEEECCCCCCCC
AANDIAALAFINTMIATAAAILSWVLAEWGLRGNPSLLGACSGCIAGLVAVTPAAGSVGV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
GGALLLGLIAGAAGLWGVVVLKRWLRTDDACDVFGVHGVCGIVGCLATGVLSAAPLGGTG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
YASGVTMGHQLWVQMLSVLVCVIWSAAAALIAYKAADLCVGLRVSEDQEREGLDVTSHGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCC
NAYNR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]