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The map label for this gene is cof [H]

Identifier: 238919020

GI number: 238919020

Start: 1084759

End: 1085571

Strand: Direct

Name: cof [H]

Synonym: NT01EI_1087

Alternate gene names: 238919020

Gene position: 1084759-1085571 (Clockwise)

Preceding gene: 238919017

Following gene: 238919021

Centisome position: 28.45

GC content: 60.39

Gene sequence:

>813_bases
TTGGCAGCGTTTGATATGGACGGCACCCTGTTGATGCCGGATCACCGCATCGGCGGGGAGACGCTGGATGTCCTGCAGCG
CCTGATGCAACAGCAGGTCGTGCTCACTTTTGCTACCGGGCGTCACTTTTTAGAGGTTAGCCGCATGATCCGCCGCCTGG
GGCTGCGGGGACACCTGATCACCGGCAACGGCACCCGGGTGCATGATCATGACGGCCAGCTGCTGTTCAGTCAGGATCTG
GTGCCGGAGGTGGCCTATGAGCTGATGCACGCCGACTGGAGCGATACTGCCTCGCTACACCTGTTTCGCGACGATGGCTG
GCTGACGGCCAGCGACTGCTCCGAACTGTTGGCGCCCCACCGGGCCGATGGCTTTTCCTACCGCCTGACGGATCTGCGTC
GCCTGCCGGCCTACGGCGTCAGCAAGGTGTGCTTTATCGATCGTCATGAACGTCTACTGTCGCTGCAGCAGCGTTTGCGC
GCCTACTTCGGCAGCGCCGCTGATCTCTGCTTCTCGGGTCATGACTCGCTGGAGGTGATCCCTCCGGGCAGCAATAAGGG
TAGTGCGCTGGCGGCTCTGTGCGATCGCCTGAATATTGGTCTGGATGAATGCATGGCGTTTGGCGATGCAATGAACGATC
GGGAGATGCTTGGCATGGTCGGTCAGGGATTCATCATGGGTAACGCGCTGACACAGCTTCGGCAGTCCTTGCCACACCTG
CAGGTGATCGGCGACTGCTGCCAGCAGGCGGTGGCGCAACAGCTTTCTCTTTGGCTAACACAATCTCACCTCACAATTCC
CCCGAATCACTGA

Upstream 100 bases:

>100_bases
ACCCTGAACTCTTTTTTCTAATTGTTCACTATTAGTTCCGTAAATGCCACGCGATACTGTATGTCATGCAGAAGAGGTCA
CAAACGGAGGCGATATGCGT

Downstream 100 bases:

>100_bases
GGCTATTCAGCCAGCCGGGCTGCGTGTTTGACTGCGCGTACCCGGTTTTCTTTTTATTTTTTCTGGCGTCGGATATCCGA
TGGAACGGGAAAAGATTGAT

Product: HAD hydrolase, IIB family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MAAFDMDGTLLMPDHRIGGETLDVLQRLMQQQVVLTFATGRHFLEVSRMIRRLGLRGHLITGNGTRVHDHDGQLLFSQDL
VPEVAYELMHADWSDTASLHLFRDDGWLTASDCSELLAPHRADGFSYRLTDLRRLPAYGVSKVCFIDRHERLLSLQQRLR
AYFGSAADLCFSGHDSLEVIPPGSNKGSALAALCDRLNIGLDECMAFGDAMNDREMLGMVGQGFIMGNALTQLRQSLPHL
QVIGDCCQQAVAQQLSLWLTQSHLTIPPNH

Sequences:

>Translated_270_residues
MAAFDMDGTLLMPDHRIGGETLDVLQRLMQQQVVLTFATGRHFLEVSRMIRRLGLRGHLITGNGTRVHDHDGQLLFSQDL
VPEVAYELMHADWSDTASLHLFRDDGWLTASDCSELLAPHRADGFSYRLTDLRRLPAYGVSKVCFIDRHERLLSLQQRLR
AYFGSAADLCFSGHDSLEVIPPGSNKGSALAALCDRLNIGLDECMAFGDAMNDREMLGMVGQGFIMGNALTQLRQSLPHL
QVIGDCCQQAVAQQLSLWLTQSHLTIPPNH
>Mature_269_residues
AAFDMDGTLLMPDHRIGGETLDVLQRLMQQQVVLTFATGRHFLEVSRMIRRLGLRGHLITGNGTRVHDHDGQLLFSQDLV
PEVAYELMHADWSDTASLHLFRDDGWLTASDCSELLAPHRADGFSYRLTDLRRLPAYGVSKVCFIDRHERLLSLQQRLRA
YFGSAADLCFSGHDSLEVIPPGSNKGSALAALCDRLNIGLDECMAFGDAMNDREMLGMVGQGFIMGNALTQLRQSLPHLQ
VIGDCCQQAVAQQLSLWLTQSHLTIPPNH

Specific function: Catalyzes the hydrolysis of 4-amino-2-methyl-5- hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2- methyl-5-hydroxymethylpyrimidine phosphate (HMP-P) [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081741, Length=269, Percent_Identity=51.3011152416357, Blast_Score=274, Evalue=5e-75,
Organism=Escherichia coli, GI48994981, Length=264, Percent_Identity=39.0151515151515, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1786982, Length=275, Percent_Identity=25.4545454545455, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF08282 Hydrolase_3 [H]

EC number: NA

Molecular weight: Translated: 30053; Mature: 29922

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAFDMDGTLLMPDHRIGGETLDVLQRLMQQQVVLTFATGRHFLEVSRMIRRLGLRGHLI
CCEECCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEE
TGNGTRVHDHDGQLLFSQDLVPEVAYELMHADWSDTASLHLFRDDGWLTASDCSELLAPH
CCCCCEEECCCCCEEECHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEHHHHHHHHCCC
RADGFSYRLTDLRRLPAYGVSKVCFIDRHERLLSLQQRLRAYFGSAADLCFSGHDSLEVI
CCCCCEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHEECCCCCEEEE
PPGSNKGSALAALCDRLNIGLDECMAFGDAMNDREMLGMVGQGFIMGNALTQLRQSLPHL
CCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCHH
QVIGDCCQQAVAQQLSLWLTQSHLTIPPNH
HHHHHHHHHHHHHHHHHHHHCCCEECCCCC
>Mature Secondary Structure 
AAFDMDGTLLMPDHRIGGETLDVLQRLMQQQVVLTFATGRHFLEVSRMIRRLGLRGHLI
CEECCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEE
TGNGTRVHDHDGQLLFSQDLVPEVAYELMHADWSDTASLHLFRDDGWLTASDCSELLAPH
CCCCCEEECCCCCEEECHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEHHHHHHHHCCC
RADGFSYRLTDLRRLPAYGVSKVCFIDRHERLLSLQQRLRAYFGSAADLCFSGHDSLEVI
CCCCCEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHEECCCCCEEEE
PPGSNKGSALAALCDRLNIGLDECMAFGDAMNDREMLGMVGQGFIMGNALTQLRQSLPHL
CCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCHH
QVIGDCCQQAVAQQLSLWLTQSHLTIPPNH
HHHHHHHHHHHHHHHHHHHHCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA