Click here to switch to the map view.

The map label for this gene is glgB [H]

Identifier: 238917956

GI number: 238917956

Start: 2116115

End: 2118058

Strand: Reverse

Name: glgB [H]

Synonym: EUBELI_02045

Alternate gene names: 238917956

Gene position: 2118058-2116115 (Counterclockwise)

Preceding gene: 238917958

Following gene: 238917955

Centisome position: 98.78

GC content: 38.84

Gene sequence:

>1944_bases
ATGGAGGATTTTATGAAGAATATGCAAAAATACGAGCAGCTGGCAATCACAGAACTGGATGCATATCTTTTTGGACAGGG
AACTCATTATGAGATATATAACAAAATGGGTGCGCATGTTGGAGTAAAAGACGGAAAAGAAGGTGTGTATTTTGCTGTGT
GGGCACCTGCTGCAAGAGAAGTCTATGTTATAGGTGAGTTTAATAACTGGAATGCATATGGTTATGATATGAAGAAGATT
TCAGATGGTGGAATATATGATTTGTTCATACCGGGAGCTAAAGCAGGGGATATGTATAAGTACCTTATTATTTCACAGAG
TGGAGAGGCACTTTACAAGGCAGATCCATATGCTAATCAGGCACAGTTAAGACCAGAGACAGCATCAATAGTTGCTGATC
TTTCAGGTTATGAATGGAAAGATTCTGAATGGGTTGAGAAGAAGAAGACGGAGAATCATCTTAAAGCTCCTCTTTCTATA
TATGAATGTCATCTTGGCTCATGGAAGAAGAAAGATGATGGAACAGAGGACGGATTTTATACATATAGAGAATTGGCACC
TGAGCTTGCAAAATATGTTTCAGATATGGGATACACACATATTGAACTTATGGGAATTGCAGAGTATCCATATGATGGCT
CATGGGGATATCAGGTAACTGGTTATTATGCACCTACAGCAAGATATGGAACACCTAAGGATTTCATGTATTTTGTAGAT
TATATGCATGAAAAAGGAATTGGTGTAATTCTTGACTGGGTACCGGCACATTTTCCAAAGGATGCACATGGACTTGCTAA
TTTCGATGGAAGCTGTGTATATGAGTATGCAGACCCTAGAAAGGGAGAGCATCCTGACTGGGGAACGAAGGTATTTGATT
ATTCCAAGAATGAAGTGTCTAACTTCCTTATTGCTAATGGAATGTTCTGGGTGGACAAGTTCCATATTGATGGTTTAAGA
GTTGATGCTGTTGCTTCAATGCTGTATTTGGATTACGGAAGGACAGAAGGAAACTGGGTTCCTAACAAATATGGTGACAA
CGGTAATCTCGAAGCAATCAGCTTTTTAAAGCATTTTAACAGCATGTTAAAGAAGAGATTCCCACAGGCAATAACTATAG
CTGAGGAGTCAACAGCATGGCCTATGGTAAGTGGTGATCCTGACAATGGGGAATGTCTTGGCTTTACATTTAAGTGGAAT
ATGGGCTGGATGCATGATTTCCTTGAATATATGAAACTCGATCCATATTTCAGAAAGTTTAATCATAGCAAGATGACATT
TTCATTGATGTATGCTTACAGCGAGAATTTCATTCTTGTGCTTTCACATGATGAGGTTGTACATCTTAAGTGTTCTATGC
TTGGTAAAATGCCTGGTGACAGGGAGGACAAGTTCAAGAATCTTAAGCTGGCATATGCATATATGTGTGGACATCCTGGC
AAGAAGCTTTTATTCATGGGACAGGAATTTGGTCAGTGGAATGAGTGGAGTGAGAAGCGTGCTCTTGACTGGTATCTGTT
AGAGGATGAAAGTCATACAGAACTTAAGGATTTTACGAAGAAGTGTCTGAAGCTTAATAAGAATTATCCATGTCTGTATG
CTACTGATTACAGCAGTGAAGGATTCAGATGGATTAATGCCAATGATAAAGATAATAGCGTTTTATCGTTTATGAGAATC
AGTCCGGATGGAAAGAAGAATCTTCTCTTTGTACTTAATTTTACACCAGTTGAAAGAGACAGCTTCAGAATAGGAGTTCC
GTTCAAGACTAAGTATAAGCTGGTTCTTGGAGATAATGAAGCTGACCAGAAGAAGACTCTTACAGCCGTTAAGGGAGATT
GTGATGGATATCCGCAGTCATTGCTTATTGACTTACATAAGTATGGAATAGCTGTGTATGAGTTTAACGGTGATGTTTCT
AAGGTTCATGCTACGAAGATATAG

Upstream 100 bases:

>100_bases
ATGAAATGTAGTAATTAATTTGCTAATGTGGTAATATACTATTAATTAGTGGGATAATCTGAGGGGACCCATGAGTATCG
TGTAGGATAATAATAATATA

Downstream 100 bases:

>100_bases
GAGAATAATATAGAGAAAGGGAGCTGGTTTGGATTTTTATTAATTCTGAATTAGCTTCCTTTTTATTTGCTATAATATAT
CTCTCAATTATCCCACCATA

Product: glycoside hydrolase family 13 protein

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE [H]

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MEDFMKNMQKYEQLAITELDAYLFGQGTHYEIYNKMGAHVGVKDGKEGVYFAVWAPAAREVYVIGEFNNWNAYGYDMKKI
SDGGIYDLFIPGAKAGDMYKYLIISQSGEALYKADPYANQAQLRPETASIVADLSGYEWKDSEWVEKKKTENHLKAPLSI
YECHLGSWKKKDDGTEDGFYTYRELAPELAKYVSDMGYTHIELMGIAEYPYDGSWGYQVTGYYAPTARYGTPKDFMYFVD
YMHEKGIGVILDWVPAHFPKDAHGLANFDGSCVYEYADPRKGEHPDWGTKVFDYSKNEVSNFLIANGMFWVDKFHIDGLR
VDAVASMLYLDYGRTEGNWVPNKYGDNGNLEAISFLKHFNSMLKKRFPQAITIAEESTAWPMVSGDPDNGECLGFTFKWN
MGWMHDFLEYMKLDPYFRKFNHSKMTFSLMYAYSENFILVLSHDEVVHLKCSMLGKMPGDREDKFKNLKLAYAYMCGHPG
KKLLFMGQEFGQWNEWSEKRALDWYLLEDESHTELKDFTKKCLKLNKNYPCLYATDYSSEGFRWINANDKDNSVLSFMRI
SPDGKKNLLFVLNFTPVERDSFRIGVPFKTKYKLVLGDNEADQKKTLTAVKGDCDGYPQSLLIDLHKYGIAVYEFNGDVS
KVHATKI

Sequences:

>Translated_647_residues
MEDFMKNMQKYEQLAITELDAYLFGQGTHYEIYNKMGAHVGVKDGKEGVYFAVWAPAAREVYVIGEFNNWNAYGYDMKKI
SDGGIYDLFIPGAKAGDMYKYLIISQSGEALYKADPYANQAQLRPETASIVADLSGYEWKDSEWVEKKKTENHLKAPLSI
YECHLGSWKKKDDGTEDGFYTYRELAPELAKYVSDMGYTHIELMGIAEYPYDGSWGYQVTGYYAPTARYGTPKDFMYFVD
YMHEKGIGVILDWVPAHFPKDAHGLANFDGSCVYEYADPRKGEHPDWGTKVFDYSKNEVSNFLIANGMFWVDKFHIDGLR
VDAVASMLYLDYGRTEGNWVPNKYGDNGNLEAISFLKHFNSMLKKRFPQAITIAEESTAWPMVSGDPDNGECLGFTFKWN
MGWMHDFLEYMKLDPYFRKFNHSKMTFSLMYAYSENFILVLSHDEVVHLKCSMLGKMPGDREDKFKNLKLAYAYMCGHPG
KKLLFMGQEFGQWNEWSEKRALDWYLLEDESHTELKDFTKKCLKLNKNYPCLYATDYSSEGFRWINANDKDNSVLSFMRI
SPDGKKNLLFVLNFTPVERDSFRIGVPFKTKYKLVLGDNEADQKKTLTAVKGDCDGYPQSLLIDLHKYGIAVYEFNGDVS
KVHATKI
>Mature_647_residues
MEDFMKNMQKYEQLAITELDAYLFGQGTHYEIYNKMGAHVGVKDGKEGVYFAVWAPAAREVYVIGEFNNWNAYGYDMKKI
SDGGIYDLFIPGAKAGDMYKYLIISQSGEALYKADPYANQAQLRPETASIVADLSGYEWKDSEWVEKKKTENHLKAPLSI
YECHLGSWKKKDDGTEDGFYTYRELAPELAKYVSDMGYTHIELMGIAEYPYDGSWGYQVTGYYAPTARYGTPKDFMYFVD
YMHEKGIGVILDWVPAHFPKDAHGLANFDGSCVYEYADPRKGEHPDWGTKVFDYSKNEVSNFLIANGMFWVDKFHIDGLR
VDAVASMLYLDYGRTEGNWVPNKYGDNGNLEAISFLKHFNSMLKKRFPQAITIAEESTAWPMVSGDPDNGECLGFTFKWN
MGWMHDFLEYMKLDPYFRKFNHSKMTFSLMYAYSENFILVLSHDEVVHLKCSMLGKMPGDREDKFKNLKLAYAYMCGHPG
KKLLFMGQEFGQWNEWSEKRALDWYLLEDESHTELKDFTKKCLKLNKNYPCLYATDYSSEGFRWINANDKDNSVLSFMRI
SPDGKKNLLFVLNFTPVERDSFRIGVPFKTKYKLVLGDNEADQKKTLTAVKGDCDGYPQSLLIDLHKYGIAVYEFNGDVS
KVHATKI

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. Transfers chain

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=674, Percent_Identity=27.4480712166172, Blast_Score=199, Evalue=5e-51,
Organism=Escherichia coli, GI1789839, Length=584, Percent_Identity=48.6301369863014, Blast_Score=585, Evalue=1e-168,
Organism=Caenorhabditis elegans, GI17554896, Length=611, Percent_Identity=27.1685761047463, Blast_Score=181, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI32564391, Length=537, Percent_Identity=27.3743016759777, Blast_Score=160, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6320826, Length=627, Percent_Identity=25.9968102073365, Blast_Score=169, Evalue=1e-42,
Organism=Drosophila melanogaster, GI28573410, Length=591, Percent_Identity=27.7495769881557, Blast_Score=196, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48 [H]

EC number: =2.4.1.18 [H]

Molecular weight: Translated: 74532; Mature: 74532

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDFMKNMQKYEQLAITELDAYLFGQGTHYEIYNKMGAHVGVKDGKEGVYFAVWAPAARE
CHHHHHHHHHHHHHHHHHCCHHEECCCCCCHHHHHCCCEECCCCCCCCEEEEEECCCCCE
VYVIGEFNNWNAYGYDMKKISDGGIYDLFIPGAKAGDMYKYLIISQSGEALYKADPYANQ
EEEEEECCCCCEECCCCEECCCCCEEEEEECCCCCCCEEEEEEEECCCCEEEECCCCCCC
AQLRPETASIVADLSGYEWKDSEWVEKKKTENHLKAPLSIYECHLGSWKKKDDGTEDGFY
CCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCH
TYRELAPELAKYVSDMGYTHIELMGIAEYPYDGSWGYQVTGYYAPTARYGTPKDFMYFVD
HHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHH
YMHEKGIGVILDWVPAHFPKDAHGLANFDGSCVYEYADPRKGEHPDWGTKVFDYSKNEVS
HHHCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEECCHHHHC
NFLIANGMFWVDKFHIDGLRVDAVASMLYLDYGRTEGNWVPNKYGDNGNLEAISFLKHFN
CEEEECCEEEEEEEEECCEEHHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH
SMLKKRFPQAITIAEESTAWPMVSGDPDNGECLGFTFKWNMGWMHDFLEYMKLDPYFRKF
HHHHHHCCCEEEEECCCCCCCEECCCCCCCCEEEEEEEECCCHHHHHHHHHHCCHHHHHC
NHSKMTFSLMYAYSENFILVLSHDEVVHLKCSMLGKMPGDREDKFKNLKLAYAYMCGHPG
CCCCEEEEEEEEECCCEEEEEECCCEEEEEEHHHCCCCCCCCHHHCCEEEEEEEECCCCC
KKLLFMGQEFGQWNEWSEKRALDWYLLEDESHTELKDFTKKCLKLNKNYPCLYATDYSSE
CEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCC
GFRWINANDKDNSVLSFMRISPDGKKNLLFVLNFTPVERDSFRIGVPFKTKYKLVLGDNE
CEEEEECCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCEEEECCCCEEEEEEECCCC
ADQKKTLTAVKGDCDGYPQSLLIDLHKYGIAVYEFNGDVSKVHATKI
CCCHHEEEEECCCCCCCCHHHHHHHHHCCEEEEEECCCEEEEEEECC
>Mature Secondary Structure
MEDFMKNMQKYEQLAITELDAYLFGQGTHYEIYNKMGAHVGVKDGKEGVYFAVWAPAARE
CHHHHHHHHHHHHHHHHHCCHHEECCCCCCHHHHHCCCEECCCCCCCCEEEEEECCCCCE
VYVIGEFNNWNAYGYDMKKISDGGIYDLFIPGAKAGDMYKYLIISQSGEALYKADPYANQ
EEEEEECCCCCEECCCCEECCCCCEEEEEECCCCCCCEEEEEEEECCCCEEEECCCCCCC
AQLRPETASIVADLSGYEWKDSEWVEKKKTENHLKAPLSIYECHLGSWKKKDDGTEDGFY
CCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCH
TYRELAPELAKYVSDMGYTHIELMGIAEYPYDGSWGYQVTGYYAPTARYGTPKDFMYFVD
HHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHH
YMHEKGIGVILDWVPAHFPKDAHGLANFDGSCVYEYADPRKGEHPDWGTKVFDYSKNEVS
HHHCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEECCHHHHC
NFLIANGMFWVDKFHIDGLRVDAVASMLYLDYGRTEGNWVPNKYGDNGNLEAISFLKHFN
CEEEECCEEEEEEEEECCEEHHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH
SMLKKRFPQAITIAEESTAWPMVSGDPDNGECLGFTFKWNMGWMHDFLEYMKLDPYFRKF
HHHHHHCCCEEEEECCCCCCCEECCCCCCCCEEEEEEEECCCHHHHHHHHHHCCHHHHHC
NHSKMTFSLMYAYSENFILVLSHDEVVHLKCSMLGKMPGDREDKFKNLKLAYAYMCGHPG
CCCCEEEEEEEEECCCEEEEEECCCEEEEEEHHHCCCCCCCCHHHCCEEEEEEEECCCCC
KKLLFMGQEFGQWNEWSEKRALDWYLLEDESHTELKDFTKKCLKLNKNYPCLYATDYSSE
CEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCC
GFRWINANDKDNSVLSFMRISPDGKKNLLFVLNFTPVERDSFRIGVPFKTKYKLVLGDNE
CEEEEECCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCEEEECCCCEEEEEEECCCC
ADQKKTLTAVKGDCDGYPQSLLIDLHKYGIAVYEFNGDVSKVHATKI
CCCHHEEEEECCCCCCCCHHHHHHHHHCCEEEEEECCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1938880 [H]