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The map label for this gene is adh1 [H]

Identifier: 238917919

GI number: 238917919

Start: 2070112

End: 2071341

Strand: Reverse

Name: adh1 [H]

Synonym: EUBELI_02006

Alternate gene names: 238917919

Gene position: 2071341-2070112 (Counterclockwise)

Preceding gene: 238917920

Following gene: 238917918

Centisome position: 96.6

GC content: 41.54

Gene sequence:

>1230_bases
ATGTACGGAGGTTTTATTATGGTTCGATTTACTTTACCAAGAGATTTGTATCATGGAAAGGGTTCTTTGGAAGCCCTCAA
GACACTTGAAGGAAAGAAAGCTATCATCTGCGTTGGTGGCGGATCTATGAAGCGTAACGGTTTCCTTCAGAAAGCAGAGG
ATTACCTCAAGGAAGCAGGAATGGAAGTTAAGCTTTTCGAGGGTATCGAGTCTGATCCATCAGTTGAGACTGTTATGAAG
GGCGCTGCTGTTATGACAGAGTTTGAGCCAGACTGGATTGTTGCTATCGGCGGAGGTTCTCCTATTGATGCTGCTAAGGC
AATGTGGATTAAGTATGAATATCCTGATACAACATTTGAAGATATGTGCAAGGTATTCGGTCTTCCTAAGCTTCGTAAGA
AAGCTCATTTCTGTGCAGTTTCATCTACATCAGGAACAGCTACAGAGGTAACAGCATTCTCTATTATTACAGATTATGAG
AAAGGTATTAAGTATCCTATAGCAGATTTCGAGATTACTCCTGATGTTGCTATTGTTGATCCTGAGCTTGCAGAGACTAT
GCCGGTTAAGCTTGTAGCACATACAGGTATGGACGCCATGACACATGCAATTGAGGCTTATGTATCAACTGCTAACTGTG
ATTACACAGATCCACTGGCACTTCATGCTATGGAGATGATTCAGGCTAATCTCGTTAAATCATACAACGGAGATATGGCA
GCAAGAGATTCAATGCACAACGCACAATGTCTTGCAGGTATGGCATTCTCTAATGCATTACTTGGTATCGTTCATTCAAT
GGCACATAAGACAGGTGCAGCATTTAAAGGTGGACATATTATCCATGGTGCAGCTAATGCTATGTATCTTCCTAAGGTTA
TCAAGTTCAATGCCAAGGATGAGACAGCAGCTAAGAGATATGCATTTATCGCTGATTTCTTACATCTTGGTGGCAATACA
CAGGATGAGAAGATTGACAATCTTATCGCTATGTTAAGAAAGATGAATGATGAACTTAATATTCCTCACTGCATCAAGAA
TTATGGTGAGGGTGGTCTCCCAGCAGAGACAGGATTTGTATCAGAGGAAGAATTCAAGGCTAAGTTACACGACATCGCAG
CCAATGCTATCCTTGATGCATGTACAGGTTCTAATCCACGTCAGCCAAGTCAGGAAGAGATGGAGAAGCTTCTTACATGC
TGCTACTATGATACAGAAGTTGACTTCTAA

Upstream 100 bases:

>100_bases
ATATTTACAAAAAATTATAATAAAATTTACTTGAATTATTATACGGGCAAGCATATAATAAAAGCATAGGGTTTGTTTAT
AATTTAACAATCAAATATTT

Downstream 100 bases:

>100_bases
TAATTACATATAAGAATAAATGTTAAGCCTTCTGGTAATAATACGGTTGTATTAATTATCAGGAGGCTTTTTATTATGGC
ATTGAATGAAAAAGAAAAGA

Product: hypothetical protein

Products: Acetyl-CoA; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: BDH [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MYGGFIMVRFTLPRDLYHGKGSLEALKTLEGKKAIICVGGGSMKRNGFLQKAEDYLKEAGMEVKLFEGIESDPSVETVMK
GAAVMTEFEPDWIVAIGGGSPIDAAKAMWIKYEYPDTTFEDMCKVFGLPKLRKKAHFCAVSSTSGTATEVTAFSIITDYE
KGIKYPIADFEITPDVAIVDPELAETMPVKLVAHTGMDAMTHAIEAYVSTANCDYTDPLALHAMEMIQANLVKSYNGDMA
ARDSMHNAQCLAGMAFSNALLGIVHSMAHKTGAAFKGGHIIHGAANAMYLPKVIKFNAKDETAAKRYAFIADFLHLGGNT
QDEKIDNLIAMLRKMNDELNIPHCIKNYGEGGLPAETGFVSEEEFKAKLHDIAANAILDACTGSNPRQPSQEEMEKLLTC
CYYDTEVDF

Sequences:

>Translated_409_residues
MYGGFIMVRFTLPRDLYHGKGSLEALKTLEGKKAIICVGGGSMKRNGFLQKAEDYLKEAGMEVKLFEGIESDPSVETVMK
GAAVMTEFEPDWIVAIGGGSPIDAAKAMWIKYEYPDTTFEDMCKVFGLPKLRKKAHFCAVSSTSGTATEVTAFSIITDYE
KGIKYPIADFEITPDVAIVDPELAETMPVKLVAHTGMDAMTHAIEAYVSTANCDYTDPLALHAMEMIQANLVKSYNGDMA
ARDSMHNAQCLAGMAFSNALLGIVHSMAHKTGAAFKGGHIIHGAANAMYLPKVIKFNAKDETAAKRYAFIADFLHLGGNT
QDEKIDNLIAMLRKMNDELNIPHCIKNYGEGGLPAETGFVSEEEFKAKLHDIAANAILDACTGSNPRQPSQEEMEKLLTC
CYYDTEVDF
>Mature_409_residues
MYGGFIMVRFTLPRDLYHGKGSLEALKTLEGKKAIICVGGGSMKRNGFLQKAEDYLKEAGMEVKLFEGIESDPSVETVMK
GAAVMTEFEPDWIVAIGGGSPIDAAKAMWIKYEYPDTTFEDMCKVFGLPKLRKKAHFCAVSSTSGTATEVTAFSIITDYE
KGIKYPIADFEITPDVAIVDPELAETMPVKLVAHTGMDAMTHAIEAYVSTANCDYTDPLALHAMEMIQANLVKSYNGDMA
ARDSMHNAQCLAGMAFSNALLGIVHSMAHKTGAAFKGGHIIHGAANAMYLPKVIKFNAKDETAAKRYAFIADFLHLGGNT
QDEKIDNLIAMLRKMNDELNIPHCIKNYGEGGLPAETGFVSEEEFKAKLHDIAANAILDACTGSNPRQPSQEEMEKLLTC
CYYDTEVDF

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=380, Percent_Identity=25, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1787493, Length=426, Percent_Identity=40.1408450704225, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1789163, Length=400, Percent_Identity=32.5, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI87082107, Length=394, Percent_Identity=34.010152284264, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI48994951, Length=359, Percent_Identity=36.4902506963788, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789386, Length=363, Percent_Identity=27.5482093663912, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17537053, Length=289, Percent_Identity=26.643598615917, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321181, Length=403, Percent_Identity=35.2357320099256, Blast_Score=199, Evalue=5e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1; 1.2.1.10 [C]

Molecular weight: Translated: 44683; Mature: 44683

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYGGFIMVRFTLPRDLYHGKGSLEALKTLEGKKAIICVGGGSMKRNGFLQKAEDYLKEAG
CCCCEEEEEEECCHHHHCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCC
MEVKLFEGIESDPSVETVMKGAAVMTEFEPDWIVAIGGGSPIDAAKAMWIKYEYPDTTFE
CEEEEECCCCCCCCHHHHHHCHHHEEECCCCEEEEECCCCCCCCCEEEEEEEECCCCCHH
DMCKVFGLPKLRKKAHFCAVSSTSGTATEVTAFSIITDYEKGIKYPIADFEITPDVAIVD
HHHHHHCCHHHHHHHHEEEEECCCCCCHHHEEEHHHHHHHHCCCCCCCCEEECCCEEEEC
PELAETMPVKLVAHTGMDAMTHAIEAYVSTANCDYTDPLALHAMEMIQANLVKSYNGDMA
CHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
ARDSMHNAQCLAGMAFSNALLGIVHSMAHKTGAAFKGGHIIHGAANAMYLPKVIKFNAKD
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEECCHHEEECCCC
ETAAKRYAFIADFLHLGGNTQDEKIDNLIAMLRKMNDELNIPHCIKNYGEGGLPAETGFV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCC
SEEEFKAKLHDIAANAILDACTGSNPRQPSQEEMEKLLTCCYYDTEVDF
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MYGGFIMVRFTLPRDLYHGKGSLEALKTLEGKKAIICVGGGSMKRNGFLQKAEDYLKEAG
CCCCEEEEEEECCHHHHCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCC
MEVKLFEGIESDPSVETVMKGAAVMTEFEPDWIVAIGGGSPIDAAKAMWIKYEYPDTTFE
CEEEEECCCCCCCCHHHHHHCHHHEEECCCCEEEEECCCCCCCCCEEEEEEEECCCCCHH
DMCKVFGLPKLRKKAHFCAVSSTSGTATEVTAFSIITDYEKGIKYPIADFEITPDVAIVD
HHHHHHCCHHHHHHHHEEEEECCCCCCHHHEEEHHHHHHHHCCCCCCCCEEECCCEEEEC
PELAETMPVKLVAHTGMDAMTHAIEAYVSTANCDYTDPLALHAMEMIQANLVKSYNGDMA
CHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
ARDSMHNAQCLAGMAFSNALLGIVHSMAHKTGAAFKGGHIIHGAANAMYLPKVIKFNAKD
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEECCHHEEECCCC
ETAAKRYAFIADFLHLGGNTQDEKIDNLIAMLRKMNDELNIPHCIKNYGEGGLPAETGFV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCC
SEEEFKAKLHDIAANAILDACTGSNPRQPSQEEMEKLLTCCYYDTEVDF
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1000 [C]

Specific activity: NA

Km value (mM): 0.15 {ethanol}} 7.05 {acetaldehyde}} 0.1 {acetaldehyde}} [C]

Substrates: Acetaldehyde; Coenzyme A; Nicotinamide adenine dinucleotide [C]

Specific reaction: Acetaldehyde + Coenzyme A + Nicotinamide adenine dinucleotide --> Acetyl-CoA + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: 8-Hydroxy quinoline-5-sulfonic acid; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH; NEM; o-Phenanthroline of Zn2+; PCMB; Pyrazole; Substituted pyrazoles 4-iodo-; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]