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The map label for this gene is gspE [C]

Identifier: 238917899

GI number: 238917899

Start: 2049694

End: 2050914

Strand: Reverse

Name: gspE [C]

Synonym: EUBELI_01986

Alternate gene names: 238917899

Gene position: 2050914-2049694 (Counterclockwise)

Preceding gene: 238917900

Following gene: 238917898

Centisome position: 95.65

GC content: 37.59

Gene sequence:

>1221_bases
ATGGAGGGATTATATCAGACTATACGTCATAAGGTGTACCAGAATCTGGATGTGGGGCATGATATTACAGATGATGAATT
ATATCAGGTCATAGACAGATGCATATATGAGGAAAGCAGGGAAAAAATAATATCAATAAGGCAAAAGGAGGAATTAAGAA
GCAGATTATATAATTCTATTAAAAAACTGGATATTTTACAGGAACTTTTAGATGATGACGAAATTACAGAAATCATGGTT
AATGGTACAGAATGCATTTTTTATGAGAAGCAGGGACGGATTAAAAAATGGGACAGGCAGTTTGAAAGTGAGGAAAAGCT
TGCTGATATTGCTCAAAGGATAGCTGCAATGTCTAACAGAATGGTTAATGAAGCATCTCCAATTGTTGATACAAGGTTAA
ATGATGGCTCACGAGTAAATATCGTATTGCCGCCAGTTGCGATTGATGGACCGGTTATAACAATAAGAAAATTTTATGAT
ACACCATTGACAATAGAACGGCTAATAGAGCTTGGTTCAATAACACCGGAAGCAGCTTGTTTTTTAGAAAAACTTGTTAA
GAGCAGATATAACATATTTATATCTGGCGGAACAGGAAGTGGTAAAACAACATTTCTTAATGTATTGTCAGATTATATAC
CAAGTGATGAAAGGGTTATAACAATTGAAGATTCCGCAGAACTTCAGTTACATAATATCAGTAATATGGTAAGACTTGAA
GCAAGGATGGCTAATGCAGAAGGTGAAAATGCTGTATCAATAAGAGATTTGATTAAATCAAGCCTTCGAATGAGACCAGA
TCGAATTGTCGTAGGTGAGGTAAGAGGAGCGGAAGCTTTGGACATGTTGCAGGCAATGAACACAGGGCATGATGGAAGTT
TGTCAACAGGACACGGCAATAGTCCGAAAGACATGCTTACCAGACTTGAGACAATGGTACTTATGGGAATGAGTATGCCG
GTTGATGCAATCAGAACACAGATAGCTTCGGCAATAGATATTATTGTTCATCTTGCAAGACAGAGAGATAAAAGCAGAAA
GGTGGTACAGATTGCAGAAGTTGGGAATTACCAAAATGGAGAAATTGAACTTACGCCATTATTTAAATATGTTGAAAAAG
GTGAGACAAAAGATGGAAGGATACTTGGTGGGCTTGTCAGGCAGAGTGCCTGTATTAAAAGGACAGAAAAGCTTGAAGCC
GCCGGAATCAGGCTTGAATGA

Upstream 100 bases:

>100_bases
ATGTACAGCCTGATATTCCTGTAGAAGACATATGGGGGAAGTTACCGTTCAGTGAATATTATGAAGATTTGCAGAGGATG
ATAAATGCAGGTGGAATTTA

Downstream 100 bases:

>100_bases
CATAAAATATATTGCAGAAGGAGTAGGAGTAATAATAATTGCAGCATATATTTTTTATGAAAATATATTTATGTCAGTAC
TTTTGATACCGTATGTTGCT

Product: pilus assembly protein CpaF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MEGLYQTIRHKVYQNLDVGHDITDDELYQVIDRCIYEESREKIISIRQKEELRSRLYNSIKKLDILQELLDDDEITEIMV
NGTECIFYEKQGRIKKWDRQFESEEKLADIAQRIAAMSNRMVNEASPIVDTRLNDGSRVNIVLPPVAIDGPVITIRKFYD
TPLTIERLIELGSITPEAACFLEKLVKSRYNIFISGGTGSGKTTFLNVLSDYIPSDERVITIEDSAELQLHNISNMVRLE
ARMANAEGENAVSIRDLIKSSLRMRPDRIVVGEVRGAEALDMLQAMNTGHDGSLSTGHGNSPKDMLTRLETMVLMGMSMP
VDAIRTQIASAIDIIVHLARQRDKSRKVVQIAEVGNYQNGEIELTPLFKYVEKGETKDGRILGGLVRQSACIKRTEKLEA
AGIRLE

Sequences:

>Translated_406_residues
MEGLYQTIRHKVYQNLDVGHDITDDELYQVIDRCIYEESREKIISIRQKEELRSRLYNSIKKLDILQELLDDDEITEIMV
NGTECIFYEKQGRIKKWDRQFESEEKLADIAQRIAAMSNRMVNEASPIVDTRLNDGSRVNIVLPPVAIDGPVITIRKFYD
TPLTIERLIELGSITPEAACFLEKLVKSRYNIFISGGTGSGKTTFLNVLSDYIPSDERVITIEDSAELQLHNISNMVRLE
ARMANAEGENAVSIRDLIKSSLRMRPDRIVVGEVRGAEALDMLQAMNTGHDGSLSTGHGNSPKDMLTRLETMVLMGMSMP
VDAIRTQIASAIDIIVHLARQRDKSRKVVQIAEVGNYQNGEIELTPLFKYVEKGETKDGRILGGLVRQSACIKRTEKLEA
AGIRLE
>Mature_406_residues
MEGLYQTIRHKVYQNLDVGHDITDDELYQVIDRCIYEESREKIISIRQKEELRSRLYNSIKKLDILQELLDDDEITEIMV
NGTECIFYEKQGRIKKWDRQFESEEKLADIAQRIAAMSNRMVNEASPIVDTRLNDGSRVNIVLPPVAIDGPVITIRKFYD
TPLTIERLIELGSITPEAACFLEKLVKSRYNIFISGGTGSGKTTFLNVLSDYIPSDERVITIEDSAELQLHNISNMVRLE
ARMANAEGENAVSIRDLIKSSLRMRPDRIVVGEVRGAEALDMLQAMNTGHDGSLSTGHGNSPKDMLTRLETMVLMGMSMP
VDAIRTQIASAIDIIVHLARQRDKSRKVVQIAEVGNYQNGEIELTPLFKYVEKGETKDGRILGGLVRQSACIKRTEKLEA
AGIRLE

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 45828; Mature: 45828

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00227 TUBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGLYQTIRHKVYQNLDVGHDITDDELYQVIDRCIYEESREKIISIRQKEELRSRLYNSI
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLDILQELLDDDEITEIMVNGTECIFYEKQGRIKKWDRQFESEEKLADIAQRIAAMSNR
HHHHHHHHHHCCCHHHHHEECCCEEEEECCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHH
MVNEASPIVDTRLNDGSRVNIVLPPVAIDGPVITIRKFYDTPLTIERLIELGSITPEAAC
HHHHCCCCCCCCCCCCCEEEEEECCEECCCCEEEEEECCCCCHHHHHHHHHCCCCHHHHH
FLEKLVKSRYNIFISGGTGSGKTTFLNVLSDYIPSDERVITIEDSAELQLHNISNMVRLE
HHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCEEEHHHHHHHHHH
ARMANAEGENAVSIRDLIKSSLRMRPDRIVVGEVRGAEALDMLQAMNTGHDGSLSTGHGN
HHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCC
SPKDMLTRLETMVLMGMSMPVDAIRTQIASAIDIIVHLARQRDKSRKVVQIAEVGNYQNG
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
EIELTPLFKYVEKGETKDGRILGGLVRQSACIKRTEKLEAAGIRLE
CEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEGLYQTIRHKVYQNLDVGHDITDDELYQVIDRCIYEESREKIISIRQKEELRSRLYNSI
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLDILQELLDDDEITEIMVNGTECIFYEKQGRIKKWDRQFESEEKLADIAQRIAAMSNR
HHHHHHHHHHCCCHHHHHEECCCEEEEECCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHH
MVNEASPIVDTRLNDGSRVNIVLPPVAIDGPVITIRKFYDTPLTIERLIELGSITPEAAC
HHHHCCCCCCCCCCCCCEEEEEECCEECCCCEEEEEECCCCCHHHHHHHHHCCCCHHHHH
FLEKLVKSRYNIFISGGTGSGKTTFLNVLSDYIPSDERVITIEDSAELQLHNISNMVRLE
HHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCEEEHHHHHHHHHH
ARMANAEGENAVSIRDLIKSSLRMRPDRIVVGEVRGAEALDMLQAMNTGHDGSLSTGHGN
HHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCC
SPKDMLTRLETMVLMGMSMPVDAIRTQIASAIDIIVHLARQRDKSRKVVQIAEVGNYQNG
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
EIELTPLFKYVEKGETKDGRILGGLVRQSACIKRTEKLEAAGIRLE
CEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]