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The map label for this gene is gltD [H]

Identifier: 238917829

GI number: 238917829

Start: 1975268

End: 1976503

Strand: Direct

Name: gltD [H]

Synonym: EUBELI_01910

Alternate gene names: 238917829

Gene position: 1975268-1976503 (Clockwise)

Preceding gene: 238917820

Following gene: 238917838

Centisome position: 92.12

GC content: 42.96

Gene sequence:

>1236_bases
ATGGCGTTACATGTTATGGACGAGGCCAACCGCTGCTTACAGTGCAAAGTGCCTCAGTGTCAGAAGGGATGTCCTATCAA
CACTAACATTCCTATGGCAATCCGTTTACTTAAAGAGAACAAGCTTAACGAAGCTGGTAAAATGCTTTTCGAGAACAACC
CACTTACTACCGTGTGCAGTCTTATCTGCAATCATGAGAACCAGTGTGAAGGTCACTGTGTACTTGGAAGAAAGGGCGCT
CCTGTACATTTTTCTACTATTGAAAATTATATCTCAAGTACATATGCCAACCAGATGACTGAAGGTCCTAAGCCTTCTAA
CGGCATGAGAGTCGCAATCATCGGTTCGGGCCCTGCCGGTATCACTATTGCAATTATTCTTGCAAGATACGGATATCAGG
TTACTATATTTGAAGGAAAGGACAAGATTGGCGGCGTATTAAGATACGGTATTCCGGAATTCAGACTTCCTAAGACTGTT
CTTGACGATATTGAATACAGACATCTTGCCTTGAAGGGCATTAAGATTCGTCCGAATACAACTATCGGTGGTGCAATCGG
AATTGATGATTTGTTCCGTGATGGCTACAAGTCTATATTTATAGGTACAGGCGTATGGAAGCCTAACTCTCTTCATATTA
AAGGAGAGACTTTCGGTAATGTCCACTTTGGTATCAATTACCTCAATAACCCTGACAGCTATCACCTTGGTGAAACTGTT
ATTGTTATCGGTGCCGGTAATGCCGCTATGGACGTTGCAAGAACTGCCAAGAGAAAAGGCTCCCGCAATGTCATCTGTTT
CTCTATCACTAAGGAAGTTGCTGCAAGCAAATATGAATTCAGCTATGCACAGCTTGAAGGAATCACATTTGAATATAACA
AACGCCCCGTTGAGATTAAGGATGACGGTGTTATATTCAGGGATATTATTGAGAATGAAGATGGAACTTTCACTGATGTA
GAAGGAACTGATACACTTCACCACTCAGACAGTGTTATCATCTCTATCAGTCAGGGACCTCAGAACCGTATTGTTAACAC
AACAACAGGTCTTAAGGCTACTGCAAGAGGACTTCTTGACGCAGACGAGACTGGTCACACAAGCCGTCCGGGTATATTTG
CATCAGGTGATGTTGTCAACGGTGCAAGAACCGTAGTAGAAGCTGTTGCACACAGCAAGATTGTGGCAGAATCAATGCAT
CAGTATATGCAGTCTCTGCCGAAGGATGAAGAATAA

Upstream 100 bases:

>100_bases
CGAGGAAAACCTGAGTTCCAGCGCCACAGACAGGTGTCGGACAGCCTGATACACACACGCAGCTTACTACTACTTAACAT
TTTTTAAAGGGGGATATATT

Downstream 100 bases:

>100_bases
AGATATTATTTTTAGCAATAAAAATCGTCTATGACACAGCTCTTAAATTGAGTTTCTGTCATAGACGATTTTATTTATCT
CTCATTAGCTTTTAATTACA

Product: oxidoreductase

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MALHVMDEANRCLQCKVPQCQKGCPINTNIPMAIRLLKENKLNEAGKMLFENNPLTTVCSLICNHENQCEGHCVLGRKGA
PVHFSTIENYISSTYANQMTEGPKPSNGMRVAIIGSGPAGITIAIILARYGYQVTIFEGKDKIGGVLRYGIPEFRLPKTV
LDDIEYRHLALKGIKIRPNTTIGGAIGIDDLFRDGYKSIFIGTGVWKPNSLHIKGETFGNVHFGINYLNNPDSYHLGETV
IVIGAGNAAMDVARTAKRKGSRNVICFSITKEVAASKYEFSYAQLEGITFEYNKRPVEIKDDGVIFRDIIENEDGTFTDV
EGTDTLHHSDSVIISISQGPQNRIVNTTTGLKATARGLLDADETGHTSRPGIFASGDVVNGARTVVEAVAHSKIVAESMH
QYMQSLPKDEE

Sequences:

>Translated_411_residues
MALHVMDEANRCLQCKVPQCQKGCPINTNIPMAIRLLKENKLNEAGKMLFENNPLTTVCSLICNHENQCEGHCVLGRKGA
PVHFSTIENYISSTYANQMTEGPKPSNGMRVAIIGSGPAGITIAIILARYGYQVTIFEGKDKIGGVLRYGIPEFRLPKTV
LDDIEYRHLALKGIKIRPNTTIGGAIGIDDLFRDGYKSIFIGTGVWKPNSLHIKGETFGNVHFGINYLNNPDSYHLGETV
IVIGAGNAAMDVARTAKRKGSRNVICFSITKEVAASKYEFSYAQLEGITFEYNKRPVEIKDDGVIFRDIIENEDGTFTDV
EGTDTLHHSDSVIISISQGPQNRIVNTTTGLKATARGLLDADETGHTSRPGIFASGDVVNGARTVVEAVAHSKIVAESMH
QYMQSLPKDEE
>Mature_410_residues
ALHVMDEANRCLQCKVPQCQKGCPINTNIPMAIRLLKENKLNEAGKMLFENNPLTTVCSLICNHENQCEGHCVLGRKGAP
VHFSTIENYISSTYANQMTEGPKPSNGMRVAIIGSGPAGITIAIILARYGYQVTIFEGKDKIGGVLRYGIPEFRLPKTVL
DDIEYRHLALKGIKIRPNTTIGGAIGIDDLFRDGYKSIFIGTGVWKPNSLHIKGETFGNVHFGINYLNNPDSYHLGETVI
VIGAGNAAMDVARTAKRKGSRNVICFSITKEVAASKYEFSYAQLEGITFEYNKRPVEIKDDGVIFRDIIENEDGTFTDVE
GTDTLHHSDSVIISISQGPQNRIVNTTTGLKATARGLLDADETGHTSRPGIFASGDVVNGARTVVEAVAHSKIVAESMHQ
YMQSLPKDEE

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=442, Percent_Identity=29.8642533936652, Blast_Score=153, Evalue=2e-37,
Organism=Escherichia coli, GI1789606, Length=432, Percent_Identity=30.5555555555556, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1788811, Length=434, Percent_Identity=29.9539170506912, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1788468, Length=421, Percent_Identity=27.790973871734, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI87082180, Length=428, Percent_Identity=31.7757009345794, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI1789243, Length=405, Percent_Identity=28.641975308642, Blast_Score=135, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI71984108, Length=441, Percent_Identity=28.7981859410431, Blast_Score=151, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17570289, Length=463, Percent_Identity=26.7818574514039, Blast_Score=132, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6320030, Length=447, Percent_Identity=26.3982102908277, Blast_Score=139, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24665547, Length=446, Percent_Identity=27.3542600896861, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24665543, Length=446, Percent_Identity=27.3542600896861, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI28574881, Length=446, Percent_Identity=27.3542600896861, Blast_Score=161, Evalue=8e-40,
Organism=Drosophila melanogaster, GI24665539, Length=446, Percent_Identity=27.3542600896861, Blast_Score=161, Evalue=8e-40,
Organism=Drosophila melanogaster, GI24640763, Length=446, Percent_Identity=27.1300448430493, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI18858217, Length=446, Percent_Identity=27.1300448430493, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 44945; Mature: 44814

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALHVMDEANRCLQCKVPQCQKGCPINTNIPMAIRLLKENKLNEAGKMLFENNPLTTVCS
CEEEEECCCCCEEEECCCCCCCCCCCCCCCCCEEEEEHHCCCCCHHHHEECCCCHHHHHH
LICNHENQCEGHCVLGRKGAPVHFSTIENYISSTYANQMTEGPKPSNGMRVAIIGSGPAG
HHHCCCCCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCC
ITIAIILARYGYQVTIFEGKDKIGGVLRYGIPEFRLPKTVLDDIEYRHLALKGIKIRPNT
HHHEEEEHHCCCEEEEECCCCCCCCEEECCCCCCCCCHHHHHCCCEEEEEEEEEEECCCC
TIGGAIGIDDLFRDGYKSIFIGTGVWKPNSLHIKGETFGNVHFGINYLNNPDSYHLGETV
CCCCCCCHHHHHHCCCCEEEEECCCCCCCEEEEECCCCCEEEEEEEECCCCCCEECCCEE
IVIGAGNAAMDVARTAKRKGSRNVICFSITKEVAASKYEFSYAQLEGITFEYNKRPVEIK
EEEECCCHHHHHHHHHHHCCCCCEEEEEECHHHHHCHHEEEEEEEECEEEEECCCCEEEC
DDGVIFRDIIENEDGTFTDVEGTDTLHHSDSVIISISQGPQNRIVNTTTGLKATARGLLD
CCCEEEEHHHCCCCCCEEECCCCCCEECCCCEEEEECCCCCCCEEECCCCCHHHHHHCCC
ADETGHTSRPGIFASGDVVNGARTVVEAVAHSKIVAESMHQYMQSLPKDEE
CCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ALHVMDEANRCLQCKVPQCQKGCPINTNIPMAIRLLKENKLNEAGKMLFENNPLTTVCS
EEEEECCCCCEEEECCCCCCCCCCCCCCCCCEEEEEHHCCCCCHHHHEECCCCHHHHHH
LICNHENQCEGHCVLGRKGAPVHFSTIENYISSTYANQMTEGPKPSNGMRVAIIGSGPAG
HHHCCCCCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCC
ITIAIILARYGYQVTIFEGKDKIGGVLRYGIPEFRLPKTVLDDIEYRHLALKGIKIRPNT
HHHEEEEHHCCCEEEEECCCCCCCCEEECCCCCCCCCHHHHHCCCEEEEEEEEEEECCCC
TIGGAIGIDDLFRDGYKSIFIGTGVWKPNSLHIKGETFGNVHFGINYLNNPDSYHLGETV
CCCCCCCHHHHHHCCCCEEEEECCCCCCCEEEEECCCCCEEEEEEEECCCCCCEECCCEE
IVIGAGNAAMDVARTAKRKGSRNVICFSITKEVAASKYEFSYAQLEGITFEYNKRPVEIK
EEEECCCHHHHHHHHHHHCCCCCEEEEEECHHHHHCHHEEEEEEEECEEEEECCCCEEEC
DDGVIFRDIIENEDGTFTDVEGTDTLHHSDSVIISISQGPQNRIVNTTTGLKATARGLLD
CCCEEEEHHHCCCCCCEEECCCCCCEECCCCEEEEECCCCCCCEEECCCCCHHHHHHCCC
ADETGHTSRPGIFASGDVVNGARTVVEAVAHSKIVAESMHQYMQSLPKDEE
CCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]