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The map label for this gene is cysU [H]

Identifier: 238917804

GI number: 238917804

Start: 1948905

End: 1949774

Strand: Reverse

Name: cysU [H]

Synonym: EUBELI_01885

Alternate gene names: 238917804

Gene position: 1949774-1948905 (Counterclockwise)

Preceding gene: 238917805

Following gene: 238917803

Centisome position: 90.93

GC content: 41.61

Gene sequence:

>870_bases
GTGATTAAGGGAAGGGGAAACGACAGAATGAAAGAGAAGAAAAGAAAGAAAAAAAGAGTAATACCAGGCTTTGGTCTTTC
ATTTGGAATAACAATTGCAATGTTAGGATTAATTGTTGTTATCCCTTTATGTACCCTTGTGATATTCTCGGCAAAGCTGT
CGTTTACGGAATTCATAACGACAGTTACAAGGCCGAGGGTGCTTTCAAGCTACAGAGTAAGTCTTGAGACTGCGTTAATC
GCGGCACTTATAGACGCAGTTATGGGAACAATTCTTGCATGGGTTCTTGTAAGATATAACTTTCCGGGTAAGCAGATTAT
GAATGGAATAATTGAACTTCCATTTGCACTGCCGACAGCTGTTGCGGGTATAGCACTTACACATCTGACAACAACTAATG
GCTGGCTTGGAGCATTTTTTGGCAAATTTGGAATCCGGATTGCATATACAAGGCTGGGAATTATATTCGCACTTATATTC
GTTGGAATACCATTCGTAGTGAGGGCGGTCCAGCCGGTGCTGGAAAAGGTGGATATCCAGTACGAGGAGGCAGCCAACAT
GCTTGGTGCAACCCACAGACAGACAGTATTCAGGGTAATACTTCCAGAGATACTTCCAGCACTTATCGGTGGATTCACAA
TGTCATTCGCAAGAGGACTTGGCGAATACGGCAGTGTCGTATTCATAGCAGGAAATACACCGTATGAGACAGAGATAGCA
CCGCTTATGATTATGTCAAAATTACAGGAATATGACTACGCAAGTGCAACATCAATAGCGCTAGTAATGCTTTTTATCGC
ATTTATTATTCTCTTTATAAATGCAGTGTTACAGAGCAGGACATCGAAGATTGTAAGTGGAATTTCATAG

Upstream 100 bases:

>100_bases
ATGTAGAACTTACGGACATAGCACATTTTGGAGGTTGGAATGAAGCTACAAAGAAGCACTTCAAGGATGGCGGAGTGTTC
GACAAGATATATGAGAAGAA

Downstream 100 bases:

>100_bases
AAAGGGGTTAGCGTATGAACATCAGAAAAGATTCAGGTCCGTTAAAATGGATTCTAATAGGTATAAGTGTGTTGTTTTTA
TTTGTGATGCTTATTCTGCC

Product: sulfate transport system permease

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MIKGRGNDRMKEKKRKKKRVIPGFGLSFGITIAMLGLIVVIPLCTLVIFSAKLSFTEFITTVTRPRVLSSYRVSLETALI
AALIDAVMGTILAWVLVRYNFPGKQIMNGIIELPFALPTAVAGIALTHLTTTNGWLGAFFGKFGIRIAYTRLGIIFALIF
VGIPFVVRAVQPVLEKVDIQYEEAANMLGATHRQTVFRVILPEILPALIGGFTMSFARGLGEYGSVVFIAGNTPYETEIA
PLMIMSKLQEYDYASATSIALVMLFIAFIILFINAVLQSRTSKIVSGIS

Sequences:

>Translated_289_residues
MIKGRGNDRMKEKKRKKKRVIPGFGLSFGITIAMLGLIVVIPLCTLVIFSAKLSFTEFITTVTRPRVLSSYRVSLETALI
AALIDAVMGTILAWVLVRYNFPGKQIMNGIIELPFALPTAVAGIALTHLTTTNGWLGAFFGKFGIRIAYTRLGIIFALIF
VGIPFVVRAVQPVLEKVDIQYEEAANMLGATHRQTVFRVILPEILPALIGGFTMSFARGLGEYGSVVFIAGNTPYETEIA
PLMIMSKLQEYDYASATSIALVMLFIAFIILFINAVLQSRTSKIVSGIS
>Mature_289_residues
MIKGRGNDRMKEKKRKKKRVIPGFGLSFGITIAMLGLIVVIPLCTLVIFSAKLSFTEFITTVTRPRVLSSYRVSLETALI
AALIDAVMGTILAWVLVRYNFPGKQIMNGIIELPFALPTAVAGIALTHLTTTNGWLGAFFGKFGIRIAYTRLGIIFALIF
VGIPFVVRAVQPVLEKVDIQYEEAANMLGATHRQTVFRVILPEILPALIGGFTMSFARGLGEYGSVVFIAGNTPYETEIA
PLMIMSKLQEYDYASATSIALVMLFIAFIILFINAVLQSRTSKIVSGIS

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=272, Percent_Identity=46.3235294117647, Blast_Score=244, Evalue=4e-66,
Organism=Escherichia coli, GI87082099, Length=277, Percent_Identity=32.129963898917, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1786980, Length=217, Percent_Identity=33.6405529953917, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787369, Length=223, Percent_Identity=27.3542600896861, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787368, Length=222, Percent_Identity=25.6756756756757, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787080, Length=223, Percent_Identity=24.2152466367713, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31646; Mature: 31646

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]