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The map label for this gene is guaA

Identifier: 238917726

GI number: 238917726

Start: 1863570

End: 1865144

Strand: Reverse

Name: guaA

Synonym: EUBELI_01806

Alternate gene names: 238917726

Gene position: 1865144-1863570 (Counterclockwise)

Preceding gene: 238917727

Following gene: 238917724

Centisome position: 86.99

GC content: 45.27

Gene sequence:

>1575_bases
ATGGATCAGGAGAAAGTTATTGTCATTGACTTCGGTGGTCAGTACAACCAGCTGGTTGCGCGCCGTGTCCGTGAATGTAA
TGTATATTGTGAGATCTATTCTTACAAGACAGACCTGGCGCAGATTAAGGCCATGAACCCTAAAGGAATTATCCTTACGG
GTGGTCCTAACAGCTGTTATGAAGATGGCGCGCCAACATGTACTAAAGAACTTTTCGAACTTGGTGTACCTGTACTTGGT
CTTTGCTATGGCGCACAGCTTATGCAGCATGTGCTTGGTGGCAAGGTTGAGAAGGCACCAGTAAGAGAGTATGGAAAGAC
AGAAACATTTGTAGATAAGAGCAGCGCTTTATTCTCAGATGTCTCAGAGAAAACTATTGTGTGGATGAGCCATTTTGACT
ACATCTCACAGATTGCACCAGGATTCAAGATTGTTGCCCACACAGCAGACTGCCCAGTTGCAGCAGCAGAGTGTACAGAG
AAGAATCTTTATGCAATCCAGTTCCACCCAGAGGTTCTTCATACTCAGGAAGGAACCAAGATGCTTCACAACTTTGTTCG
TGGAGTATGCGGATGTGCCGGAACATGGAAGATGGATGCATTTGTTGAGAATACAATTAAGGAAATCCGAGAGAAGGTTG
GCAGTGGCAAGGTTTTACTTGCTCTTTCAGGTGGTGTTGATTCATCTGTTGCAGCCGGACTTCTTTCAAGAGCTATTGGA
AAGCAGCTTACATGCGTATTCGTAGATCACGGTCTTCTCCGTAAAGATGAAGGCGATGAAGTTGAGAGCGTATTTGGACC
AGAAGGACAGTTCGACCTTAACTTTATAAGAGTTAATGCACAGGAGAGATATTACTCTAAGCTTGCAGGCGTTACAGAGC
CAGAAGCTAAGAGAAAGATTATTGGGGAAGAGTTTATCCGTGTATTTGAGGAAGAAGCCAAGAAGATTGGTGCAGTTGAC
TTCCTTGCACAGGGAACTATATATCCAGACGTTGTAGAAAGCGGACTTGGCGGTGAATCAGCCGTAATCAAGTCTCACCA
CAATGTAGGAGGACTTCCTGATTTCGTTGACTTCAAGGAAATCATTGAGCCACTCCGCAACCTTTTCAAGGATGAGGTTC
GTAAGGCTGGTCTTGAGCTTGGAATACCAGAACATCTTGTATTCCGTCAGCCATTCCCAGGTCCGGGACTTGGAATCAGA
ATTATCGGCGAGGTAACAGCTGATAAGGTTCGTATCGTTCAGGATGCTGACTTTATCTACCGTTCAGAGGTTGACAAGGC
CGTAGCTGAGTACAAGAAAGCTAACGGCAAGGCTCCAGCATGGATGCCAAACCAGTATTTCGCAGCCCTCACTAACATGC
GTTCCGTTGGTGTTATGGGTGACGAAAGAACTTACGATTATGCGGTTGCACTTCGTGCAGTCAACACAATTGATTTCATG
ACAGCAGAGAGCGCAGAGATTCCATTTGAGGTTCTCCAGACAGTCATGAGCAGAATTATAAATGAGGTGCGTGGGGTCAA
CAGAGTATTCTATGATCTGACTAGTAAACCACCTGGAACGATTGAGTTTGAATAA

Upstream 100 bases:

>100_bases
GTAGAATTAGCAAGATAGGAATTGGGGAGAATTAAGGCTTGTCATAAGTGGCAAGTTTAACTATAATGTATATATAAATA
CACAAGATTGGAGATTGATC

Downstream 100 bases:

>100_bases
TTTTCAAAAGCTCGCAAAGCCTAGTAAAATGGGCATTTGCGAGCTTGTTTTTTGCCTTGTGGTACTAAAATGGTACTAAA
GTGAGCTGTGCGTGAATAGT

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MDQEKVIVIDFGGQYNQLVARRVRECNVYCEIYSYKTDLAQIKAMNPKGIILTGGPNSCYEDGAPTCTKELFELGVPVLG
LCYGAQLMQHVLGGKVEKAPVREYGKTETFVDKSSALFSDVSEKTIVWMSHFDYISQIAPGFKIVAHTADCPVAAAECTE
KNLYAIQFHPEVLHTQEGTKMLHNFVRGVCGCAGTWKMDAFVENTIKEIREKVGSGKVLLALSGGVDSSVAAGLLSRAIG
KQLTCVFVDHGLLRKDEGDEVESVFGPEGQFDLNFIRVNAQERYYSKLAGVTEPEAKRKIIGEEFIRVFEEEAKKIGAVD
FLAQGTIYPDVVESGLGGESAVIKSHHNVGGLPDFVDFKEIIEPLRNLFKDEVRKAGLELGIPEHLVFRQPFPGPGLGIR
IIGEVTADKVRIVQDADFIYRSEVDKAVAEYKKANGKAPAWMPNQYFAALTNMRSVGVMGDERTYDYAVALRAVNTIDFM
TAESAEIPFEVLQTVMSRIINEVRGVNRVFYDLTSKPPGTIEFE

Sequences:

>Translated_524_residues
MDQEKVIVIDFGGQYNQLVARRVRECNVYCEIYSYKTDLAQIKAMNPKGIILTGGPNSCYEDGAPTCTKELFELGVPVLG
LCYGAQLMQHVLGGKVEKAPVREYGKTETFVDKSSALFSDVSEKTIVWMSHFDYISQIAPGFKIVAHTADCPVAAAECTE
KNLYAIQFHPEVLHTQEGTKMLHNFVRGVCGCAGTWKMDAFVENTIKEIREKVGSGKVLLALSGGVDSSVAAGLLSRAIG
KQLTCVFVDHGLLRKDEGDEVESVFGPEGQFDLNFIRVNAQERYYSKLAGVTEPEAKRKIIGEEFIRVFEEEAKKIGAVD
FLAQGTIYPDVVESGLGGESAVIKSHHNVGGLPDFVDFKEIIEPLRNLFKDEVRKAGLELGIPEHLVFRQPFPGPGLGIR
IIGEVTADKVRIVQDADFIYRSEVDKAVAEYKKANGKAPAWMPNQYFAALTNMRSVGVMGDERTYDYAVALRAVNTIDFM
TAESAEIPFEVLQTVMSRIINEVRGVNRVFYDLTSKPPGTIEFE
>Mature_524_residues
MDQEKVIVIDFGGQYNQLVARRVRECNVYCEIYSYKTDLAQIKAMNPKGIILTGGPNSCYEDGAPTCTKELFELGVPVLG
LCYGAQLMQHVLGGKVEKAPVREYGKTETFVDKSSALFSDVSEKTIVWMSHFDYISQIAPGFKIVAHTADCPVAAAECTE
KNLYAIQFHPEVLHTQEGTKMLHNFVRGVCGCAGTWKMDAFVENTIKEIREKVGSGKVLLALSGGVDSSVAAGLLSRAIG
KQLTCVFVDHGLLRKDEGDEVESVFGPEGQFDLNFIRVNAQERYYSKLAGVTEPEAKRKIIGEEFIRVFEEEAKKIGAVD
FLAQGTIYPDVVESGLGGESAVIKSHHNVGGLPDFVDFKEIIEPLRNLFKDEVRKAGLELGIPEHLVFRQPFPGPGLGIR
IIGEVTADKVRIVQDADFIYRSEVDKAVAEYKKANGKAPAWMPNQYFAALTNMRSVGVMGDERTYDYAVALRAVNTIDFM
TAESAEIPFEVLQTVMSRIINEVRGVNRVFYDLTSKPPGTIEFE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=565, Percent_Identity=35.3982300884956, Blast_Score=309, Evalue=5e-84,
Organism=Escherichia coli, GI1788854, Length=534, Percent_Identity=50.187265917603, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI1789760, Length=167, Percent_Identity=25.1497005988024, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71992710, Length=574, Percent_Identity=36.411149825784, Blast_Score=315, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI133901714, Length=574, Percent_Identity=36.411149825784, Blast_Score=315, Evalue=4e-86,
Organism=Caenorhabditis elegans, GI71992717, Length=574, Percent_Identity=36.411149825784, Blast_Score=315, Evalue=4e-86,
Organism=Saccharomyces cerevisiae, GI6323873, Length=529, Percent_Identity=46.124763705104, Blast_Score=455, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6322638, Length=179, Percent_Identity=27.9329608938547, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI281365319, Length=549, Percent_Identity=36.0655737704918, Blast_Score=293, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_EUBE2 (C4Z3X1)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002931243.1
- ProteinModelPortal:   C4Z3X1
- GeneID:   7957864
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_01806
- OMA:   MKLVEPL
- ProtClustDB:   CLSK2548418
- HAMAP:   MF_00344
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase

EC number: =6.3.5.2

Molecular weight: Translated: 57914; Mature: 57914

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 82-82 ACT_SITE 169-169 ACT_SITE 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQEKVIVIDFGGQYNQLVARRVRECNVYCEIYSYKTDLAQIKAMNPKGIILTGGPNSCY
CCCCCEEEEECCCCHHHHHHHHHHHCEEEEEEEECHHHHHHHEECCCCEEEEECCCCCCC
EDGAPTCTKELFELGVPVLGLCYGAQLMQHVLGGKVEKAPVREYGKTETFVDKSSALFSD
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHC
VSEKTIVWMSHFDYISQIAPGFKIVAHTADCPVAAAECTEKNLYAIQFHPEVLHTQEGTK
CCCCEEEEHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCCCEEEEEECCHHHHCCHHHH
MLHNFVRGVCGCAGTWKMDAFVENTIKEIREKVGSGKVLLALSGGVDSSVAAGLLSRAIG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHC
KQLTCVFVDHGLLRKDEGDEVESVFGPEGQFDLNFIRVNAQERYYSKLAGVTEPEAKRKI
CCEEEEEECCCCCCCCCCCHHHHHCCCCCCEEEEEEEECCHHHHHHHHCCCCCCHHHHHH
IGEEFIRVFEEEAKKIGAVDFLAQGTIYPDVVESGLGGESAVIKSHHNVGGLPDFVDFKE
HHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHCCCCCCHHHEECCCCCCCCCCHHHHHH
IIEPLRNLFKDEVRKAGLELGIPEHLVFRQPFPGPGLGIRIIGEVTADKVRIVQDADFIY
HHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCEEEEEECCCCCEEEEECCHHHH
RSEVDKAVAEYKKANGKAPAWMPNQYFAALTNMRSVGVMGDERTYDYAVALRAVNTIDFM
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHE
TAESAEIPFEVLQTVMSRIINEVRGVNRVFYDLTSKPPGTIEFE
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECC
>Mature Secondary Structure
MDQEKVIVIDFGGQYNQLVARRVRECNVYCEIYSYKTDLAQIKAMNPKGIILTGGPNSCY
CCCCCEEEEECCCCHHHHHHHHHHHCEEEEEEEECHHHHHHHEECCCCEEEEECCCCCCC
EDGAPTCTKELFELGVPVLGLCYGAQLMQHVLGGKVEKAPVREYGKTETFVDKSSALFSD
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHC
VSEKTIVWMSHFDYISQIAPGFKIVAHTADCPVAAAECTEKNLYAIQFHPEVLHTQEGTK
CCCCEEEEHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCCCEEEEEECCHHHHCCHHHH
MLHNFVRGVCGCAGTWKMDAFVENTIKEIREKVGSGKVLLALSGGVDSSVAAGLLSRAIG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHC
KQLTCVFVDHGLLRKDEGDEVESVFGPEGQFDLNFIRVNAQERYYSKLAGVTEPEAKRKI
CCEEEEEECCCCCCCCCCCHHHHHCCCCCCEEEEEEEECCHHHHHHHHCCCCCCHHHHHH
IGEEFIRVFEEEAKKIGAVDFLAQGTIYPDVVESGLGGESAVIKSHHNVGGLPDFVDFKE
HHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHCCCCCCHHHEECCCCCCCCCCHHHHHH
IIEPLRNLFKDEVRKAGLELGIPEHLVFRQPFPGPGLGIRIIGEVTADKVRIVQDADFIY
HHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCEEEEEECCCCCEEEEECCHHHH
RSEVDKAVAEYKKANGKAPAWMPNQYFAALTNMRSVGVMGDERTYDYAVALRAVNTIDFM
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHE
TAESAEIPFEVLQTVMSRIINEVRGVNRVFYDLTSKPPGTIEFE
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA