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The map label for this gene is vsr [H]

Identifier: 238917710

GI number: 238917710

Start: 1850036

End: 1850473

Strand: Reverse

Name: vsr [H]

Synonym: EUBELI_01790

Alternate gene names: 238917710

Gene position: 1850473-1850036 (Counterclockwise)

Preceding gene: 238917711

Following gene: 238917709

Centisome position: 86.3

GC content: 36.3

Gene sequence:

>438_bases
GTGGCGGATGTTTTAACAAAAGAACAGCGGCATAAGAATATGAAAAATATTCATGGAAAGGATACCAAAATAGAAGTTAT
ATTGAGAAAGGCTTTATGGAGCAAAGGATACCGGTATCGTAAGAATTATAAAAAATTGCCGGGCAGCCCGGATATTGCAC
TGACAAAGTATAAGATTGCAATTTTCTGTGATGGAGAATTCTTTCATGGGAAAGATTGGGATACTTTAAAGAAGAGACTG
GAAAAGAGCAACAACAGTGAGTTTTGGATTAATAAAATTTCCCGTAACATAGAGCGGGATGATGAGATAAATAAAAAATT
GGACTTTGAGGGCTGGACTGTAATCCGGTTTTGGGGTGAAGATATAAAGAAACATACGGATGAGTGTGTTAAGGTTGTGG
AAGAGACAATTTTTGATAGTATGGAATTTGAAGAGTAG

Upstream 100 bases:

>100_bases
CTTGATGTAAAGACTCTTATGAGCATAAATGTCAGACAGGAAATGAAAGAGATAGAAAAATTGGCAAAATTGATTGAAAA
CTGATTGTTGAGGTGATTTT

Downstream 100 bases:

>100_bases
AAGAAAAATATAGATGGAGTGGATTTATATGCTAGCGTGATAAAATGGGTAATCATGATGGAAAAAGATATTTTTTGGCA
GAATTACATATGGGCAATAT

Product: hypothetical protein

Products: DNA [C]

Alternate protein names: V.NmeDIP [H]

Number of amino acids: Translated: 145; Mature: 144

Protein sequence:

>145_residues
MADVLTKEQRHKNMKNIHGKDTKIEVILRKALWSKGYRYRKNYKKLPGSPDIALTKYKIAIFCDGEFFHGKDWDTLKKRL
EKSNNSEFWINKISRNIERDDEINKKLDFEGWTVIRFWGEDIKKHTDECVKVVEETIFDSMEFEE

Sequences:

>Translated_145_residues
MADVLTKEQRHKNMKNIHGKDTKIEVILRKALWSKGYRYRKNYKKLPGSPDIALTKYKIAIFCDGEFFHGKDWDTLKKRL
EKSNNSEFWINKISRNIERDDEINKKLDFEGWTVIRFWGEDIKKHTDECVKVVEETIFDSMEFEE
>Mature_144_residues
ADVLTKEQRHKNMKNIHGKDTKIEVILRKALWSKGYRYRKNYKKLPGSPDIALTKYKIAIFCDGEFFHGKDWDTLKKRLE
KSNNSEFWINKISRNIERDDEINKKLDFEGWTVIRFWGEDIKKHTDECVKVVEETIFDSMEFEE

Specific function: May nick NmeDIP sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=118, Percent_Identity=37.2881355932203, Blast_Score=79, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 17435; Mature: 17304

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADVLTKEQRHKNMKNIHGKDTKIEVILRKALWSKGYRYRKNYKKLPGSPDIALTKYKIA
CCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEEEEE
IFCDGEFFHGKDWDTLKKRLEKSNNSEFWINKISRNIERDDEINKKLDFEGWTVIRFWGE
EEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHCCCCCCCEEEEEEHHH
DIKKHTDECVKVVEETIFDSMEFEE
HHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ADVLTKEQRHKNMKNIHGKDTKIEVILRKALWSKGYRYRKNYKKLPGSPDIALTKYKIA
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEEEEE
IFCDGEFFHGKDWDTLKKRLEKSNNSEFWINKISRNIERDDEINKKLDFEGWTVIRFWGE
EEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHCCCCCCCEEEEEEHHH
DIKKHTDECVKVVEETIFDSMEFEE
HHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10671450 [H]