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The map label for this gene is dfrA13 [H]

Identifier: 238917701

GI number: 238917701

Start: 1842139

End: 1842612

Strand: Direct

Name: dfrA13 [H]

Synonym: EUBELI_01781

Alternate gene names: 238917701

Gene position: 1842139-1842612 (Clockwise)

Preceding gene: 238917700

Following gene: 238917702

Centisome position: 85.91

GC content: 32.28

Gene sequence:

>474_bases
ATGATTGGATTGATTGTTGCAAGGTCAAAAAATAATGTTATTGGTAGAAATGGTGAGATTCCTTGGAAAATCAAAGGTGA
GCAAAAATTTTTTAAGGAACTTACAATCGGAAATATTGTAGTTATGGGAAGAAAATCTTATGAAGAAATTGGTCATCCAC
TTCCAAATCGCCAAAATATAATAGTATCAAAGACAATGAAATATGAAGGCGAAAACCTAATTACTGTATCATCTTTGGCA
GAAGCATTGGCACTTTCTGACAATTCAAATATTTATATAGCAGGAGGGTATGGAGTATACAAAGAGGCTATCCCTTTTGT
TGATGTAATGTACATTACAGAAGTAGATATGAATATCGAAGATGGAGATGTATTTTTTCCAGAGTTTGATGTAAATGATT
TCGATTTGATTATAGGTGAAACATTTGGCGAGAATACTAAGTATACGAGGACTACATACATAAGAAAGAAGTAG

Upstream 100 bases:

>100_bases
TGAATTCTTTGCAGAAGAAGATTTATAAGCAATTCCCCAAACCCCTTTTGGGAATCTTTTGTTTACAAAAAAAACAAATA
AAAAACTTTAGAGGTAGAAA

Downstream 100 bases:

>100_bases
CCATTGTAACCAGGACCTTCCAAGAACGTCCAGACAAATACAATATACTGCAATTTGAATTTGATACTGATCAATCTTGC
TTGTTGAGTGTTATTCAAGA

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type XIII; DHFRXIII [H]

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MIGLIVARSKNNVIGRNGEIPWKIKGEQKFFKELTIGNIVVMGRKSYEEIGHPLPNRQNIIVSKTMKYEGENLITVSSLA
EALALSDNSNIYIAGGYGVYKEAIPFVDVMYITEVDMNIEDGDVFFPEFDVNDFDLIIGETFGENTKYTRTTYIRKK

Sequences:

>Translated_157_residues
MIGLIVARSKNNVIGRNGEIPWKIKGEQKFFKELTIGNIVVMGRKSYEEIGHPLPNRQNIIVSKTMKYEGENLITVSSLA
EALALSDNSNIYIAGGYGVYKEAIPFVDVMYITEVDMNIEDGDVFFPEFDVNDFDLIIGETFGENTKYTRTTYIRKK
>Mature_157_residues
MIGLIVARSKNNVIGRNGEIPWKIKGEQKFFKELTIGNIVVMGRKSYEEIGHPLPNRQNIIVSKTMKYEGENLITVSSLA
EALALSDNSNIYIAGGYGVYKEAIPFVDVMYITEVDMNIEDGDVFFPEFDVNDFDLIIGETFGENTKYTRTTYIRKK

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=158, Percent_Identity=31.6455696202532, Blast_Score=75, Evalue=2e-14,
Organism=Homo sapiens, GI28827793, Length=159, Percent_Identity=30.8176100628931, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1786233, Length=152, Percent_Identity=32.8947368421053, Blast_Score=97, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17505899, Length=167, Percent_Identity=29.940119760479, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324810, Length=137, Percent_Identity=32.1167883211679, Blast_Score=65, Evalue=4e-12,
Organism=Drosophila melanogaster, GI116008026, Length=142, Percent_Identity=38.7323943661972, Blast_Score=83, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24647458, Length=142, Percent_Identity=38.7323943661972, Blast_Score=83, Evalue=8e-17,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 17785; Mature: 17785

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGLIVARSKNNVIGRNGEIPWKIKGEQKFFKELTIGNIVVMGRKSYEEIGHPLPNRQNI
CEEEEEEECCCCCCCCCCCCCEEECCHHHHHHHCCCCEEEEEECCCHHHHCCCCCCCCEE
IVSKTMKYEGENLITVSSLAEALALSDNSNIYIAGGYGVYKEAIPFVDVMYITEVDMNIE
EEEEEEEECCCCEEEHHHHHHHHEECCCCCEEEEECCCCHHHCCCEEEEEEEEEECCCCC
DGDVFFPEFDVNDFDLIIGETFGENTKYTRTTYIRKK
CCCEECCCCCCCCEEEEEECCCCCCCCEEEEEEEEEC
>Mature Secondary Structure
MIGLIVARSKNNVIGRNGEIPWKIKGEQKFFKELTIGNIVVMGRKSYEEIGHPLPNRQNI
CEEEEEEECCCCCCCCCCCCCEEECCHHHHHHHCCCCEEEEEECCCHHHHCCCCCCCCEE
IVSKTMKYEGENLITVSSLAEALALSDNSNIYIAGGYGVYKEAIPFVDVMYITEVDMNIE
EEEEEEEECCCCEEEHHHHHHHHEECCCCCEEEEECCCCHHHCCCEEEEEEEEEECCCCC
DGDVFFPEFDVNDFDLIIGETFGENTKYTRTTYIRKK
CCCEECCCCCCCCEEEEEECCCCCCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10639362 [H]