Click here to switch to the map view.

The map label for this gene is spaK [H]

Identifier: 238917579

GI number: 238917579

Start: 1717937

End: 1719313

Strand: Reverse

Name: spaK [H]

Synonym: EUBELI_01658

Alternate gene names: 238917579

Gene position: 1719313-1717937 (Counterclockwise)

Preceding gene: 238917580

Following gene: 238917578

Centisome position: 80.18

GC content: 39.07

Gene sequence:

>1377_bases
ATGGGAAGAGTAAAGAGAAGCAATGCACTCAGCTGGATTTTTATGAGATATGTACTGGTCATGCTTGGATCATTAGTAGG
TTTGGTGATTGTTGCTTGGCTGCTGCTGTCCCTTTTGATTAGTGTGGGCTGTATTTATCCGGCAAATTATGCAGAGCAAA
AGATTAATGAAGCATATGATACGATTCTATGTGCAGATAAAGTGACTGCTGAGATGATTCCCGCACCTTGTGATTATGTA
ATCTTTTCAGAGAATGGAGAGAAAATAGGTGGAGATCTGTCAGAACAATACGAACAGATAGCATGGAATGTTGCAAAGTA
CGGAAACGCATCCGGGAAATATTTTTATAAAGTAATTGTAAGGGAAAATGAATATGTTGTATTGCAATATCGCCTGACAC
CTCAATATCATTCAGCTTTTTTGAGGGAGCATTTTATAGGACCACAGAATGTGATGAGTATTATGTCTGTGATTGGAGCG
GTAGCGATTATCATCATTCCGTCCATACGTTTTGGAAAAAGAATCAAAAAGCAGATGCAGCCTGTATTAGACGCAATCGG
ACAAATAAAGGATCAAAATCTGGAATATGAGACATCCTGTTCCGGTATCAAAGAGTTTGATGACTGTTTATCGGCAATCG
ATGATATGCGAGATGCATTGCGAGAATCTTTAGAAAAGCAGTGGAAAACAGAGCAGGAAAAGAAACAACAGATGTCTGCT
TTGGCGCATGATATTAAAACACCTCTTACGATTGTACGGGGAAATGCAGAACTACTTTCAGAGACTGAACTGACCACAGA
ACAGAGGAATAATATCACTTATGTTTTGAACGGCACAACACAGATACAAAGTTATGTAAAACAGTTGATAGATGTCACAA
AATCATGGAATTGCAGTGATGTTACCTATACAACGGTGAGGTTAGAAGATTTTTTCGCAGATATAAAGGAACAGGCACTC
AGACTGGTAGAAATCTATCACCAGAAAATAGATTGGAAGGCGGGACAAAGTGATAAAAAGGTAACGATTGCTTATGATCC
AATGTTCCGGGCCGTAATGAATATGATTCAGAATGCAGTGGAGCATACAAAAGAAAATGGAATCATTTATATTGACGCAA
AGGAGCAGGATGGCCGGCTGACATTCATTGTGGAGGATAGCGGATCAGGATTTACAAAAGAAGCATTATTGCATGGCACA
GAGCAGTTTTTTATGGATGACACAAGTAGAAATGGCGAAGCCCATTATGGGATGGGACTATTTTTTGCAAAAACCGTGGC
TGAAAAATATGGTGGAGGTATTAAACTTTCAAATTCTGAAAATACAGGTGGGGCGAGGGTAGAAATATTTTTCCTGAGTA
GTCAAGAAACAAGCTAA

Upstream 100 bases:

>100_bases
GTAATGACAGTACTATAATCACGCATATCAAAAATATAAGAGCAAAATTTGCGGATTATGATTATATGCCAATAAAAACA
GTCTGGGGGATTGGATATAA

Downstream 100 bases:

>100_bases
ATAGACCGCAATTAAGCTATGTAAAAGAAATTTTACATGCTTTTATCTAATATGTAAAAGCCTTTTGACAGACATTACTA
CTACGTTTGCTGTAATCTCA

Product: hypothetical protein

Products: NA

Alternate protein names: Subtilin biosynthesis sensor protein spaK [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MGRVKRSNALSWIFMRYVLVMLGSLVGLVIVAWLLLSLLISVGCIYPANYAEQKINEAYDTILCADKVTAEMIPAPCDYV
IFSENGEKIGGDLSEQYEQIAWNVAKYGNASGKYFYKVIVRENEYVVLQYRLTPQYHSAFLREHFIGPQNVMSIMSVIGA
VAIIIIPSIRFGKRIKKQMQPVLDAIGQIKDQNLEYETSCSGIKEFDDCLSAIDDMRDALRESLEKQWKTEQEKKQQMSA
LAHDIKTPLTIVRGNAELLSETELTTEQRNNITYVLNGTTQIQSYVKQLIDVTKSWNCSDVTYTTVRLEDFFADIKEQAL
RLVEIYHQKIDWKAGQSDKKVTIAYDPMFRAVMNMIQNAVEHTKENGIIYIDAKEQDGRLTFIVEDSGSGFTKEALLHGT
EQFFMDDTSRNGEAHYGMGLFFAKTVAEKYGGGIKLSNSENTGGARVEIFFLSSQETS

Sequences:

>Translated_458_residues
MGRVKRSNALSWIFMRYVLVMLGSLVGLVIVAWLLLSLLISVGCIYPANYAEQKINEAYDTILCADKVTAEMIPAPCDYV
IFSENGEKIGGDLSEQYEQIAWNVAKYGNASGKYFYKVIVRENEYVVLQYRLTPQYHSAFLREHFIGPQNVMSIMSVIGA
VAIIIIPSIRFGKRIKKQMQPVLDAIGQIKDQNLEYETSCSGIKEFDDCLSAIDDMRDALRESLEKQWKTEQEKKQQMSA
LAHDIKTPLTIVRGNAELLSETELTTEQRNNITYVLNGTTQIQSYVKQLIDVTKSWNCSDVTYTTVRLEDFFADIKEQAL
RLVEIYHQKIDWKAGQSDKKVTIAYDPMFRAVMNMIQNAVEHTKENGIIYIDAKEQDGRLTFIVEDSGSGFTKEALLHGT
EQFFMDDTSRNGEAHYGMGLFFAKTVAEKYGGGIKLSNSENTGGARVEIFFLSSQETS
>Mature_457_residues
GRVKRSNALSWIFMRYVLVMLGSLVGLVIVAWLLLSLLISVGCIYPANYAEQKINEAYDTILCADKVTAEMIPAPCDYVI
FSENGEKIGGDLSEQYEQIAWNVAKYGNASGKYFYKVIVRENEYVVLQYRLTPQYHSAFLREHFIGPQNVMSIMSVIGAV
AIIIIPSIRFGKRIKKQMQPVLDAIGQIKDQNLEYETSCSGIKEFDDCLSAIDDMRDALRESLEKQWKTEQEKKQQMSAL
AHDIKTPLTIVRGNAELLSETELTTEQRNNITYVLNGTTQIQSYVKQLIDVTKSWNCSDVTYTTVRLEDFFADIKEQALR
LVEIYHQKIDWKAGQSDKKVTIAYDPMFRAVMNMIQNAVEHTKENGIIYIDAKEQDGRLTFIVEDSGSGFTKEALLHGTE
QFFMDDTSRNGEAHYGMGLFFAKTVAEKYGGGIKLSNSENTGGARVEIFFLSSQETS

Specific function: Member of the two-component regulatory system spaK/spaR involved in the regulation of the biosynthesis of lantibiotic subtilin. SpaK may function as a membrane-associated protein kinase that phosphorylates spaR in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790861, Length=227, Percent_Identity=29.0748898678414, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR008358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51825; Mature: 51694

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRVKRSNALSWIFMRYVLVMLGSLVGLVIVAWLLLSLLISVGCIYPANYAEQKINEAYD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
TILCADKVTAEMIPAPCDYVIFSENGEKIGGDLSEQYEQIAWNVAKYGNASGKYFYKVIV
HHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEE
RENEYVVLQYRLTPQYHSAFLREHFIGPQNVMSIMSVIGAVAIIIIPSIRFGKRIKKQMQ
ECCCEEEEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
PVLDAIGQIKDQNLEYETSCSGIKEFDDCLSAIDDMRDALRESLEKQWKTEQEKKQQMSA
HHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LAHDIKTPLTIVRGNAELLSETELTTEQRNNITYVLNGTTQIQSYVKQLIDVTKSWNCSD
HHHHHCCCEEEEECCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCC
VTYTTVRLEDFFADIKEQALRLVEIYHQKIDWKAGQSDKKVTIAYDPMFRAVMNMIQNAV
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHH
EHTKENGIIYIDAKEQDGRLTFIVEDSGSGFTKEALLHGTEQFFMDDTSRNGEAHYGMGL
HHHHCCCEEEEECCCCCCEEEEEEECCCCCCHHHHHHHCCHHHHCCCCCCCCCCCCCHHH
FFAKTVAEKYGGGIKLSNSENTGGARVEIFFLSSQETS
HHHHHHHHHHCCCEEECCCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure 
GRVKRSNALSWIFMRYVLVMLGSLVGLVIVAWLLLSLLISVGCIYPANYAEQKINEAYD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
TILCADKVTAEMIPAPCDYVIFSENGEKIGGDLSEQYEQIAWNVAKYGNASGKYFYKVIV
HHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEE
RENEYVVLQYRLTPQYHSAFLREHFIGPQNVMSIMSVIGAVAIIIIPSIRFGKRIKKQMQ
ECCCEEEEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
PVLDAIGQIKDQNLEYETSCSGIKEFDDCLSAIDDMRDALRESLEKQWKTEQEKKQQMSA
HHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LAHDIKTPLTIVRGNAELLSETELTTEQRNNITYVLNGTTQIQSYVKQLIDVTKSWNCSD
HHHHHCCCEEEEECCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCC
VTYTTVRLEDFFADIKEQALRLVEIYHQKIDWKAGQSDKKVTIAYDPMFRAVMNMIQNAV
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHH
EHTKENGIIYIDAKEQDGRLTFIVEDSGSGFTKEALLHGTEQFFMDDTSRNGEAHYGMGL
HHHHCCCEEEEECCCCCCEEEEEEECCCCCCHHHHHHHCCHHHHCCCCCCCCCCCCCHHH
FFAKTVAEKYGGGIKLSNSENTGGARVEIFFLSSQETS
HHHHHHHHHHCCCEEECCCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8439156; 8085823 [H]