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The map label for this gene is vanS [H]

Identifier: 238917426

GI number: 238917426

Start: 1559469

End: 1560986

Strand: Reverse

Name: vanS [H]

Synonym: EUBELI_01504

Alternate gene names: 238917426

Gene position: 1560986-1559469 (Counterclockwise)

Preceding gene: 238917427

Following gene: 238917425

Centisome position: 72.8

GC content: 34.45

Gene sequence:

>1518_bases
ATGGAGAGAAATATGAACGCACAATATGTACGGAAAAGAATCGGAATATATAAGGAACTTGCGATGGTAATTGTGATATC
AATTACTATCGCAGTTTTTGCTTCATTAGGAGTGTTTATTGTATGCAGCCATTATGGAGTTATGGTCGATGTAATAGAAG
ATACATCTGATTATAAGAAAAGATACGATATTCAGGTAATGAATATGGTAAGAGAATTAGACAGCTCACTTGATACTGCA
ATGGAAGAGATAACTGCCAATAGTGGTGAGGACGAGAAAACAATAGGAGATGCGATGAAGTATCTTCTTGGGAATAACAG
AATGCCATTTGCTTCCGATGAAGTAAGTAATATGATAATCACAATAACGAACAGAAATGGACAGGTATTGTGGAAGACTT
CGGAAGATGGAGAAATCGTTGATTATAAGAAAAATAAAGTCAGTGTTGACATTTCCCAATATATAACTAATTCATATGAC
GAAGAAAGTCTTACATATACATTTGTATATACTAAGAGCGTTTCAAAAATGCTTTACTATATTATATTCGAGACAGAGGT
TACACCGGAATACCTGTATGCTGATATAAAGCTGAAGCTTATGTGTATTGCGATTAGTTCAGTTATATTTATAATAAGCG
TTTTTCTGCTTACAAAGAAAAGAATTGATTATATCAGATATCTGTCAACGGTTGTAGATGAGATATCAAAAGGAAATCTT
AATGCCAGCATAGATAAAAAGGGCAATGACGAGATTACTCTTATAGCTGAACAGATTGATGATATGCAGTACAGTCTGAG
GAAGATGATGCAGGAGGAGCGTGAGAACGACAGAAAGAATATGGAGCTGATTACCAATCTTTCACATGATATCAAGACGC
CAATGACAATAATAACGGGATATCTGGATGTTGTTATAAGTGGCAAGTATTCAACTGATGAGGAGCGAGATGATTATATC
CGGAGGGCATTTGGACAGGTTGAAAAGATTAATGTAATGATTCATAAAATCTTCAGGCTTGCAAGAAATGAACAGGTTTC
CATGAACGAAGAGAACATGGAAAAATGTAATATTTCATTGCTTCTTAAACAGGATATTCTTGAATTTGACGGAATTGCAC
AGAAGGAAGGCAAATGCTTTGAAACAGATATACCTAATGAATCAGTGATGATTAATATTAATATTGACAGGATAAGAGAA
GTATTTGACAATGTTTTAATGAATTCTATCAAGTATTCATCGGACAATTCAACGATAAATGTGTATATGAGTGAGACACC
TGAATCTGTTCATATAAAATTCTGCAACAGGACAGATAGTGATAAAGCACCTGACTGCGAAAGAATATTTGACAAATTCT
ACAGGGCAGACCATGCAAGAAATTCAAGTATATCCGGCAACGGACTTGGACTTTCGATTGTAAAGGAAACAATAGAAAGC
TTTAACGGAAGAGTATGGGCTGAGTATAATGATGGAATATTTGCAATTAATATAGAACTTAAGAAGAATGATATGTGA

Upstream 100 bases:

>100_bases
ACAGTATAAAGTATGATTATACACATCTTAATGGATATGGATATTATGCATATGGTGTAGGTGTATATGAAAAAGGCGTT
GAATTAGGTTACTGGTAATC

Downstream 100 bases:

>100_bases
ATCTGATTATGAAATGAGGAGGATATTATGGCATCTGTTTTAGTGGTTGATGATGAAGAAGATATTAGAGAGCTTGTCGG
AATCTATATAAAAAATGAGG

Product: hypothetical protein

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MERNMNAQYVRKRIGIYKELAMVIVISITIAVFASLGVFIVCSHYGVMVDVIEDTSDYKKRYDIQVMNMVRELDSSLDTA
MEEITANSGEDEKTIGDAMKYLLGNNRMPFASDEVSNMIITITNRNGQVLWKTSEDGEIVDYKKNKVSVDISQYITNSYD
EESLTYTFVYTKSVSKMLYYIIFETEVTPEYLYADIKLKLMCIAISSVIFIISVFLLTKKRIDYIRYLSTVVDEISKGNL
NASIDKKGNDEITLIAEQIDDMQYSLRKMMQEERENDRKNMELITNLSHDIKTPMTIITGYLDVVISGKYSTDEERDDYI
RRAFGQVEKINVMIHKIFRLARNEQVSMNEENMEKCNISLLLKQDILEFDGIAQKEGKCFETDIPNESVMININIDRIRE
VFDNVLMNSIKYSSDNSTINVYMSETPESVHIKFCNRTDSDKAPDCERIFDKFYRADHARNSSISGNGLGLSIVKETIES
FNGRVWAEYNDGIFAINIELKKNDM

Sequences:

>Translated_505_residues
MERNMNAQYVRKRIGIYKELAMVIVISITIAVFASLGVFIVCSHYGVMVDVIEDTSDYKKRYDIQVMNMVRELDSSLDTA
MEEITANSGEDEKTIGDAMKYLLGNNRMPFASDEVSNMIITITNRNGQVLWKTSEDGEIVDYKKNKVSVDISQYITNSYD
EESLTYTFVYTKSVSKMLYYIIFETEVTPEYLYADIKLKLMCIAISSVIFIISVFLLTKKRIDYIRYLSTVVDEISKGNL
NASIDKKGNDEITLIAEQIDDMQYSLRKMMQEERENDRKNMELITNLSHDIKTPMTIITGYLDVVISGKYSTDEERDDYI
RRAFGQVEKINVMIHKIFRLARNEQVSMNEENMEKCNISLLLKQDILEFDGIAQKEGKCFETDIPNESVMININIDRIRE
VFDNVLMNSIKYSSDNSTINVYMSETPESVHIKFCNRTDSDKAPDCERIFDKFYRADHARNSSISGNGLGLSIVKETIES
FNGRVWAEYNDGIFAINIELKKNDM
>Mature_505_residues
MERNMNAQYVRKRIGIYKELAMVIVISITIAVFASLGVFIVCSHYGVMVDVIEDTSDYKKRYDIQVMNMVRELDSSLDTA
MEEITANSGEDEKTIGDAMKYLLGNNRMPFASDEVSNMIITITNRNGQVLWKTSEDGEIVDYKKNKVSVDISQYITNSYD
EESLTYTFVYTKSVSKMLYYIIFETEVTPEYLYADIKLKLMCIAISSVIFIISVFLLTKKRIDYIRYLSTVVDEISKGNL
NASIDKKGNDEITLIAEQIDDMQYSLRKMMQEERENDRKNMELITNLSHDIKTPMTIITGYLDVVISGKYSTDEERDDYI
RRAFGQVEKINVMIHKIFRLARNEQVSMNEENMEKCNISLLLKQDILEFDGIAQKEGKCFETDIPNESVMININIDRIRE
VFDNVLMNSIKYSSDNSTINVYMSETPESVHIKFCNRTDSDKAPDCERIFDKFYRADHARNSSISGNGLGLSIVKETIES
FNGRVWAEYNDGIFAINIELKKNDM

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=286, Percent_Identity=22.7272727272727, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1790346, Length=265, Percent_Identity=24.5283018867925, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786600, Length=263, Percent_Identity=21.6730038022814, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786783, Length=294, Percent_Identity=20.4081632653061, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1788279, Length=297, Percent_Identity=24.9158249158249, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI87082128, Length=311, Percent_Identity=22.508038585209, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 58203; Mature: 58203

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERNMNAQYVRKRIGIYKELAMVIVISITIAVFASLGVFIVCSHYGVMVDVIEDTSDYKK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCHHHHH
RYDIQVMNMVRELDSSLDTAMEEITANSGEDEKTIGDAMKYLLGNNRMPFASDEVSNMII
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
TITNRNGQVLWKTSEDGEIVDYKKNKVSVDISQYITNSYDEESLTYTFVYTKSVSKMLYY
EEECCCCEEEEEECCCCCEEEEECCEEEEEHHHHHCCCCCCCCEEEEEEEHHHHHHEEEE
IIFETEVTPEYLYADIKLKLMCIAISSVIFIISVFLLTKKRIDYIRYLSTVVDEISKGNL
EEEECCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NASIDKKGNDEITLIAEQIDDMQYSLRKMMQEERENDRKNMELITNLSHDIKTPMTIITG
CEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHHHHH
YLDVVISGKYSTDEERDDYIRRAFGQVEKINVMIHKIFRLARNEQVSMNEENMEKCNISL
HHHHEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCEE
LLKQDILEFDGIAQKEGKCFETDIPNESVMININIDRIREVFDNVLMNSIKYSSDNSTIN
EEHHHHHHHCCCCCCCCCEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHEECCCCCEEE
VYMSETPESVHIKFCNRTDSDKAPDCERIFDKFYRADHARNSSISGNGLGLSIVKETIES
EEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
FNGRVWAEYNDGIFAINIELKKNDM
HCCEEEEEECCCEEEEEEEEECCCC
>Mature Secondary Structure
MERNMNAQYVRKRIGIYKELAMVIVISITIAVFASLGVFIVCSHYGVMVDVIEDTSDYKK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCHHHHH
RYDIQVMNMVRELDSSLDTAMEEITANSGEDEKTIGDAMKYLLGNNRMPFASDEVSNMII
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
TITNRNGQVLWKTSEDGEIVDYKKNKVSVDISQYITNSYDEESLTYTFVYTKSVSKMLYY
EEECCCCEEEEEECCCCCEEEEECCEEEEEHHHHHCCCCCCCCEEEEEEEHHHHHHEEEE
IIFETEVTPEYLYADIKLKLMCIAISSVIFIISVFLLTKKRIDYIRYLSTVVDEISKGNL
EEEECCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NASIDKKGNDEITLIAEQIDDMQYSLRKMMQEERENDRKNMELITNLSHDIKTPMTIITG
CEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHHHHH
YLDVVISGKYSTDEERDDYIRRAFGQVEKINVMIHKIFRLARNEQVSMNEENMEKCNISL
HHHHEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCEE
LLKQDILEFDGIAQKEGKCFETDIPNESVMININIDRIREVFDNVLMNSIKYSSDNSTIN
EEHHHHHHHCCCCCCCCCEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHEECCCCCEEE
VYMSETPESVHIKFCNRTDSDKAPDCERIFDKFYRADHARNSSISGNGLGLSIVKETIES
EEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
FNGRVWAEYNDGIFAINIELKKNDM
HCCEEEEEECCCEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]