Click here to switch to the map view.

The map label for this gene is dacF [H]

Identifier: 238917380

GI number: 238917380

Start: 1510967

End: 1512079

Strand: Reverse

Name: dacF [H]

Synonym: EUBELI_01457

Alternate gene names: 238917380

Gene position: 1512079-1510967 (Counterclockwise)

Preceding gene: 238917381

Following gene: 238917379

Centisome position: 70.52

GC content: 37.2

Gene sequence:

>1113_bases
ATGGGATTCGATAATTATGATGAAGAAAAAATACGCCGCAACAGAAAAAGAAAAATGCTGTTGATGAAGAAGAAAAGACA
GCAGATTTTAAGAAGAAGAATTATGATAACGGCTTTAATGGTTATTGCGATTATACTTGCTGTTATTATTATCAATTCTG
TTTCAGGAATAAAGAAAACATTTAAACAGAAGGAAGCATTTGCATCGGATGTGATTAGTGAAACACAGCCAGAGGAAGAA
ACAGAGAAGCCTACAGAGCCGCCTCTTATATATGCCCAGATGGCATCTGATTATTTTGATCTTACATCTGAAAGTAAGGT
TTCTTCGCCATATGCTGCTCTTTTAGATGTTGATAATCATAAAATAATTGCAGGAAAACAGGCAGATACCAGAATGTATC
CGGCGTCTATGACAAAGGTAATGACTCTTATAGTTGTAACTGATAATATAGATAAGATTCCACAGACATATACTTTTGGA
TTTGAAATGCTTAACAGATTATTCAGGGAAGAAGCTTCTGTAGCAGGATTTCTTGATGGAGAGACTGTTGATGTGGAAGA
TTTACTTTATGGATTGATACTTCCGTCAGGTGCTGATGCGGCAGAGGCACTTGCAATAATGGTTGCAGGTTCTAATGAAG
AATTTGCTAAGCTGATGAATGCAAAATGTAATGAACTTGGTTTAAAACATACACGATTTGCAAACCCTACAGGACTTTAT
GACGAGGAACAGTATACAACTCCTGCAGAGATGGCTATGATAATGGAGTATGCAATGAGGGATGAACTAAGGGCAAAAGT
GCTTGGAACATATCAGTATAAAACCAAAGCAACAGCACAGCATCCAGATGGAATACAGCTTACAAGTACAATGTATTCAA
GGATATATGGCAATGAAGCACCCGGGGTTATTGTTAAAGGTGGCAAGACAGGATTTATCAGCCAGGCAGGAAGCTGTCTT
GTAAGCTATGCGGTCACTAATAAAGGGCATGCTTATGTATTCTGCACAGGCGGGGCTACATATAAATGGGCACCGGTTTA
TGACGAAATATATGTATATAAGAATATAATTCCTGAATCAGAGAAAAATTTAGAAGCAGAAACAAAAAAATAA

Upstream 100 bases:

>100_bases
ATTTGGGACTCGAACTTGTAAGACTTATCAAAGGGGAAGAACTGGCAGAAAAATTAAAAGCTTCTACCCAGTATATTTAG
TATGAGGGAGAGAAATTACG

Downstream 100 bases:

>100_bases
CTACTAATAATTAACAGCCAGAATATGTCATAATAATCAGAATTAACAGATACATAATAATATCAAGACGATAAGTTATG
GCGCCATAACTTTTCGGCTT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MGFDNYDEEKIRRNRKRKMLLMKKKRQQILRRRIMITALMVIAIILAVIIINSVSGIKKTFKQKEAFASDVISETQPEEE
TEKPTEPPLIYAQMASDYFDLTSESKVSSPYAALLDVDNHKIIAGKQADTRMYPASMTKVMTLIVVTDNIDKIPQTYTFG
FEMLNRLFREEASVAGFLDGETVDVEDLLYGLILPSGADAAEALAIMVAGSNEEFAKLMNAKCNELGLKHTRFANPTGLY
DEEQYTTPAEMAMIMEYAMRDELRAKVLGTYQYKTKATAQHPDGIQLTSTMYSRIYGNEAPGVIVKGGKTGFISQAGSCL
VSYAVTNKGHAYVFCTGGATYKWAPVYDEIYVYKNIIPESEKNLEAETKK

Sequences:

>Translated_370_residues
MGFDNYDEEKIRRNRKRKMLLMKKKRQQILRRRIMITALMVIAIILAVIIINSVSGIKKTFKQKEAFASDVISETQPEEE
TEKPTEPPLIYAQMASDYFDLTSESKVSSPYAALLDVDNHKIIAGKQADTRMYPASMTKVMTLIVVTDNIDKIPQTYTFG
FEMLNRLFREEASVAGFLDGETVDVEDLLYGLILPSGADAAEALAIMVAGSNEEFAKLMNAKCNELGLKHTRFANPTGLY
DEEQYTTPAEMAMIMEYAMRDELRAKVLGTYQYKTKATAQHPDGIQLTSTMYSRIYGNEAPGVIVKGGKTGFISQAGSCL
VSYAVTNKGHAYVFCTGGATYKWAPVYDEIYVYKNIIPESEKNLEAETKK
>Mature_369_residues
GFDNYDEEKIRRNRKRKMLLMKKKRQQILRRRIMITALMVIAIILAVIIINSVSGIKKTFKQKEAFASDVISETQPEEET
EKPTEPPLIYAQMASDYFDLTSESKVSSPYAALLDVDNHKIIAGKQADTRMYPASMTKVMTLIVVTDNIDKIPQTYTFGF
EMLNRLFREEASVAGFLDGETVDVEDLLYGLILPSGADAAEALAIMVAGSNEEFAKLMNAKCNELGLKHTRFANPTGLYD
EEQYTTPAEMAMIMEYAMRDELRAKVLGTYQYKTKATAQHPDGIQLTSTMYSRIYGNEAPGVIVKGGKTGFISQAGSCLV
SYAVTNKGHAYVFCTGGATYKWAPVYDEIYVYKNIIPESEKNLEAETKK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=238, Percent_Identity=34.0336134453782, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1787062, Length=155, Percent_Identity=38.7096774193548, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87082022, Length=226, Percent_Identity=30.0884955752212, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 41454; Mature: 41323

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFDNYDEEKIRRNRKRKMLLMKKKRQQILRRRIMITALMVIAIILAVIIINSVSGIKKT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKQKEAFASDVISETQPEEETEKPTEPPLIYAQMASDYFDLTSESKVSSPYAALLDVDNH
HHHHHHHHHHHHHCCCCCHHHCCCCCCCEEHHHHHHHHHCCCCCCCCCCCCEEEEECCCC
KIIAGKQADTRMYPASMTKVMTLIVVTDNIDKIPQTYTFGFEMLNRLFREEASVAGFLDG
EEEECCCCCCCCCHHHHHHHEEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHEEECCC
ETVDVEDLLYGLILPSGADAAEALAIMVAGSNEEFAKLMNAKCNELGLKHTRFANPTGLY
CEECHHHHHHHHHCCCCCCHHHEEEEEEECCCHHHHHHHHCCHHHCCCCCCCCCCCCCCC
DEEQYTTPAEMAMIMEYAMRDELRAKVLGTYQYKTKATAQHPDGIQLTSTMYSRIYGNEA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCC
PGVIVKGGKTGFISQAGSCLVSYAVTNKGHAYVFCTGGATYKWAPVYDEIYVYKNIIPES
CCEEEECCCCCCHHHHCCEEEEEEEECCCCEEEEECCCCCEEECCHHHHHHHHHHCCCCC
EKNLEAETKK
CCCCCCCCCC
>Mature Secondary Structure 
GFDNYDEEKIRRNRKRKMLLMKKKRQQILRRRIMITALMVIAIILAVIIINSVSGIKKT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKQKEAFASDVISETQPEEETEKPTEPPLIYAQMASDYFDLTSESKVSSPYAALLDVDNH
HHHHHHHHHHHHHCCCCCHHHCCCCCCCEEHHHHHHHHHCCCCCCCCCCCCEEEEECCCC
KIIAGKQADTRMYPASMTKVMTLIVVTDNIDKIPQTYTFGFEMLNRLFREEASVAGFLDG
EEEECCCCCCCCCHHHHHHHEEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHEEECCC
ETVDVEDLLYGLILPSGADAAEALAIMVAGSNEEFAKLMNAKCNELGLKHTRFANPTGLY
CEECHHHHHHHHHCCCCCCHHHEEEEEEECCCHHHHHHHHCCHHHCCCCCCCCCCCCCCC
DEEQYTTPAEMAMIMEYAMRDELRAKVLGTYQYKTKATAQHPDGIQLTSTMYSRIYGNEA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCC
PGVIVKGGKTGFISQAGSCLVSYAVTNKGHAYVFCTGGATYKWAPVYDEIYVYKNIIPES
CCEEEECCCCCCHHHHCCEEEEEEEECCCCEEEEECCCCCEEECCHHHHHHHHHHCCCCC
EKNLEAETKK
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]