Click here to switch to the map view.

The map label for this gene is tig [H]

Identifier: 238917378

GI number: 238917378

Start: 1509133

End: 1510422

Strand: Reverse

Name: tig [H]

Synonym: EUBELI_01455

Alternate gene names: 238917378

Gene position: 1510422-1509133 (Counterclockwise)

Preceding gene: 238917379

Following gene: 238917377

Centisome position: 70.44

GC content: 38.91

Gene sequence:

>1290_bases
ATGAGTTTCACAGTTGAGAATTTAGAAGAGAAGAATATGGTTAAACTTGTTATTGAGTCTACTGCTGAGGAGTTTGAGGC
AGGTCTTAATACTGCATATAATAAGAATAAGAGCAAGATCAGCCTTCCTGGTTTCAGAAAAGGTAAAGCACCTAGAAAGA
TGATTGAGAAGATGTATGGCGCAGAAGTATTCTACGAAGATGCTGCTAATTCAATCATTCCAGATGCATATGCTAAGGCT
GCTGATGAGTGCGGTCTTGAACTTGTTTCACAGCCTAAGATCAATGTAACACAGTTAGAGGCTGGAAAGCCATTTATCTT
CGAAGCTGTTGTTGCTACTAAGCCAGAAGTTGAACTTGGACAGTACAAGGGTGTTGAGGTAACTAAGGCTGATACAGAAG
CAACAGATGCTGATGTTGAAGAAGAGTTAAAGAGAGTACAGGATCAGAATTCAAGAACAGTAGCTGTAACAGACAGAGCT
GTTAAGGATGGCGATAATACAGTTATTGATTTCGAAGGATTTGTTGATGGTGTTGCATTTGAAGGTGGAAAGGGAACAGA
TTACCCACTTACAATCGGATCTCATTCATTCATTGATACATTCGAGGATCAGATTATCGGTATGAATATCGGTGATGAGA
AAGAAATCAATGTTACATTCCCAGAAGAGTATCATGTAGATGATCTTAAGGGTAAGCCAGCTATGTTTAAGGTTTCTGTT
AAGGAAATTAAGGAGAAGCAGCTTCCAGAGCTTAATGATGAATTTGCTCAGGATGTATCTGATTTTGATACAATTGCTGA
ATATAAGGATGATTTAAAGAAAAAGATTGCTGACAGAAAGTCAAGAGAAGCTAAGGCTAAGCAGGAAGACGAAGCAATCG
CTAAGATTATTGAAGACTCTAAGATGGATATTCCAGATGCTATGGTTGATACACAGGTTAACAGAATGGTTGAGGATTTC
GCTCAGAGATTACAGCAGCAGGGATTATCAGTTGAGCAGTACTTCCAGTACACAGGCATGACAGCTGATAAGATTATGGA
AGAAATGAAGCCGGAGGCTGTTAAGAGAATTCAGTCAAGACTTGTTCTTGAAGCAGTTGTTAAGGCTGAGAATATTGAGA
CTTCAGACGAAGATTTCGAAGCAGAGTTAAAGAAGATGGCTGAAGCATACAAGATGGAACTTGATCAGATTAAGGAATTC
ATGGGAGACTATGAGAAGAAGCAGATTAAGGAAGATCTTGCAATTCAGAAGGCTGTAGATGTTATCGTAGGATCAGTTGT
TGAAAAGTAA

Upstream 100 bases:

>100_bases
TTTAAAAATAGTACTGGTAATCTAAAAACAGTTGTGTTATAATTCGTGGGTATGTTGTACGATAGTAACAATACATATTT
CATAGGAGGATTAATTTATA

Downstream 100 bases:

>100_bases
TTGAATCTTAAAGAAACATTTTAGTATATTGGAGGTTGTGTAATGAGTTTTGTACCTTATGTCATTGAACAGAACAGCAG
AGGCGAAAGATCATATGATA

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSFTVENLEEKNMVKLVIESTAEEFEAGLNTAYNKNKSKISLPGFRKGKAPRKMIEKMYGAEVFYEDAANSIIPDAYAKA
ADECGLELVSQPKINVTQLEAGKPFIFEAVVATKPEVELGQYKGVEVTKADTEATDADVEEELKRVQDQNSRTVAVTDRA
VKDGDNTVIDFEGFVDGVAFEGGKGTDYPLTIGSHSFIDTFEDQIIGMNIGDEKEINVTFPEEYHVDDLKGKPAMFKVSV
KEIKEKQLPELNDEFAQDVSDFDTIAEYKDDLKKKIADRKSREAKAKQEDEAIAKIIEDSKMDIPDAMVDTQVNRMVEDF
AQRLQQQGLSVEQYFQYTGMTADKIMEEMKPEAVKRIQSRLVLEAVVKAENIETSDEDFEAELKKMAEAYKMELDQIKEF
MGDYEKKQIKEDLAIQKAVDVIVGSVVEK

Sequences:

>Translated_429_residues
MSFTVENLEEKNMVKLVIESTAEEFEAGLNTAYNKNKSKISLPGFRKGKAPRKMIEKMYGAEVFYEDAANSIIPDAYAKA
ADECGLELVSQPKINVTQLEAGKPFIFEAVVATKPEVELGQYKGVEVTKADTEATDADVEEELKRVQDQNSRTVAVTDRA
VKDGDNTVIDFEGFVDGVAFEGGKGTDYPLTIGSHSFIDTFEDQIIGMNIGDEKEINVTFPEEYHVDDLKGKPAMFKVSV
KEIKEKQLPELNDEFAQDVSDFDTIAEYKDDLKKKIADRKSREAKAKQEDEAIAKIIEDSKMDIPDAMVDTQVNRMVEDF
AQRLQQQGLSVEQYFQYTGMTADKIMEEMKPEAVKRIQSRLVLEAVVKAENIETSDEDFEAELKKMAEAYKMELDQIKEF
MGDYEKKQIKEDLAIQKAVDVIVGSVVEK
>Mature_428_residues
SFTVENLEEKNMVKLVIESTAEEFEAGLNTAYNKNKSKISLPGFRKGKAPRKMIEKMYGAEVFYEDAANSIIPDAYAKAA
DECGLELVSQPKINVTQLEAGKPFIFEAVVATKPEVELGQYKGVEVTKADTEATDADVEEELKRVQDQNSRTVAVTDRAV
KDGDNTVIDFEGFVDGVAFEGGKGTDYPLTIGSHSFIDTFEDQIIGMNIGDEKEINVTFPEEYHVDDLKGKPAMFKVSVK
EIKEKQLPELNDEFAQDVSDFDTIAEYKDDLKKKIADRKSREAKAKQEDEAIAKIIEDSKMDIPDAMVDTQVNRMVEDFA
QRLQQQGLSVEQYFQYTGMTADKIMEEMKPEAVKRIQSRLVLEAVVKAENIETSDEDFEAELKKMAEAYKMELDQIKEFM
GDYEKKQIKEDLAIQKAVDVIVGSVVEK

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=383, Percent_Identity=30.2872062663185, Blast_Score=164, Evalue=9e-42,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 48274; Mature: 48143

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS50059 FKBP_PPIASE ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFTVENLEEKNMVKLVIESTAEEFEAGLNTAYNKNKSKISLPGFRKGKAPRKMIEKMYG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHC
AEVFYEDAANSIIPDAYAKAADECGLELVSQPKINVTQLEAGKPFIFEAVVATKPEVELG
CHHHHHHHCCCCCCHHHHHHHHHHCHHHHCCCCCCEEEECCCCCCEEHHHHHCCCCCCCC
QYKGVEVTKADTEATDADVEEELKRVQDQNSRTVAVTDRAVKDGDNTVIDFEGFVDGVAF
CCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEECHHHHCCCCCEEEECCCCCCEEE
EGGKGTDYPLTIGSHSFIDTFEDQIIGMNIGDEKEINVTFPEEYHVDDLKGKPAMFKVSV
CCCCCCCCCEECCCCHHHHHHHHHEEECCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEH
KEIKEKQLPELNDEFAQDVSDFDTIAEYKDDLKKKIADRKSREAKAKQEDEAIAKIIEDS
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KMDIPDAMVDTQVNRMVEDFAQRLQQQGLSVEQYFQYTGMTADKIMEEMKPEAVKRIQSR
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHCHHHHHHHHHH
LVLEAVVKAENIETSDEDFEAELKKMAEAYKMELDQIKEFMGDYEKKQIKEDLAIQKAVD
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIVGSVVEK
HHHHHHHCC
>Mature Secondary Structure 
SFTVENLEEKNMVKLVIESTAEEFEAGLNTAYNKNKSKISLPGFRKGKAPRKMIEKMYG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHC
AEVFYEDAANSIIPDAYAKAADECGLELVSQPKINVTQLEAGKPFIFEAVVATKPEVELG
CHHHHHHHCCCCCCHHHHHHHHHHCHHHHCCCCCCEEEECCCCCCEEHHHHHCCCCCCCC
QYKGVEVTKADTEATDADVEEELKRVQDQNSRTVAVTDRAVKDGDNTVIDFEGFVDGVAF
CCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEECHHHHCCCCCEEEECCCCCCEEE
EGGKGTDYPLTIGSHSFIDTFEDQIIGMNIGDEKEINVTFPEEYHVDDLKGKPAMFKVSV
CCCCCCCCCEECCCCHHHHHHHHHEEECCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEH
KEIKEKQLPELNDEFAQDVSDFDTIAEYKDDLKKKIADRKSREAKAKQEDEAIAKIIEDS
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KMDIPDAMVDTQVNRMVEDFAQRLQQQGLSVEQYFQYTGMTADKIMEEMKPEAVKRIQSR
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHCHHHHHHHHHH
LVLEAVVKAENIETSDEDFEAELKKMAEAYKMELDQIKEFMGDYEKKQIKEDLAIQKAVD
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIVGSVVEK
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA