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The map label for this gene is rho [H]

Identifier: 238917370

GI number: 238917370

Start: 1499829

End: 1501286

Strand: Reverse

Name: rho [H]

Synonym: EUBELI_01447

Alternate gene names: 238917370

Gene position: 1501286-1499829 (Counterclockwise)

Preceding gene: 238917374

Following gene: 238917368

Centisome position: 70.02

GC content: 38.41

Gene sequence:

>1458_bases
ATGAATACAGAGGTGGATTATATGAAGGCAAAGTTAGAAACATTGTCAGTTAGTGTATTGCGTGAATTTGCAAAGGATAA
GAATATTAAGAATATCTCGACAATGAGAAAGAGTGAACTTATAGAGGCAATTATCGCAGCGGCAGAAGGCGAGGAAAAGA
ACGAGCCGGCTGAAGTTGCAAGTGTTGTTGCAGAAGATGAAGCTGACGGACAGAACAATCATAAGACATCAGAATATTCA
CATCAGAAGTCTGAACAGTATGTTAAAGAAGAACATAAAGATAATTATCATGCACAGTCACATAAGGGGTCGGAGCAGTC
AAAGCAGGAACAGGATATGCCTATGGCAGATGGTATTCTTGAAGTTTTGTCAGAAGGATATGGATTTATAAGATGTGAGA
ATTTTCTTCCCGGAGAGAATGATGTATATGTTTCTCCGTCACAGATAAGAAAGTTTAATTTAAAGACAGGTGATATTATT
CAGGGACCTAAGAGAATGAAAACATCGGGTGAAAAGTTTTCGGCACTTATGTATCTGGAAAGCGTTAATGGTAAGGATCC
TTCTGTTGCACAGAAAAGACCGGCATTTGAAAGCCTTACGCCAATTTTCCCTAATGAAAGAATTCATCTTGAAAAGAATT
CAAGTACAGTGGCAATGAGAATGGTTGACCTTATATCTCCTATAGGAAAAGGACAGCGAGGCATGATTGTTTCGCAGCCT
AAGTCGGGTAAGACAACACTTCTTAAGCAGATTGCCAATGCAGTAACTAAGAATAACCCAGAGATGCATCTTATGATTCT
TCTTATTGACGAACGACCAGAGGAAGTAACAGATATCAAAGAGTCAATAGTTGGAGATAATGTAGAAGTTATATATTCAA
CATTTGACGAGTTGCCAGAAAGACATAAGAGAGTATCAGAGATGGTTATTGAAAGAGCTAAGAGACTTGTTGAGCAGAAG
ACAGATGTTATAATACTTCTTGATAGTATTACAAGACTTGCAAGGGCATATAACCTTACAGTTCAGGCAAGTGGACGAAC
TCTTTCCGGAGGTCTTGATCCGGCAGCACTCCACATGCCTAAGAGATTCTTTGGTGCGGCAAGAAATATGCGTGAGGGTG
GAAGTCTTACAATTTTGGCAACAGCACTTGTTGAGACTGGAAGCAAGATGGATGATGTTGTATTCGAGGAGTTTAAGGGT
ACGGGTAATATGGAACTTGTCCTTGATAGAAAATTGTCAGAAAAGAGAGTATTTCCAGCTATTGATATAGCAAAGTCAAG
TACAAGACGCGAAGATTTACTGCTTAACAAAGAAGAACAGGAAGCTGTTGATATTATGAGAAAGGCACTTAACGGTTTAA
AGACGGATGAGGCAGCAGAGAGAATTCTTGACCTTTTTGTTAAAACCAAGAATAATGAAGAACTTGTAAGAACAGTAATT
AAGAATCATTATATTTAA

Upstream 100 bases:

>100_bases
CTAAAAAAAGCATTTTTAGTCTTTTCTTTTAGAATAATATGTTGTATAATTAATACATCTTTGATGCGTTTTCTGCATCA
TTTTCCCTATTGATTTTTAG

Downstream 100 bases:

>100_bases
AGTATACTTTCTTTCTGTAGCATTTTTATAACTTTTTGAAAAAGTGCTTGCATATAATGTAACATCGTGATACAATATGA
AAGCTGTTTATGACAGATAA

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MNTEVDYMKAKLETLSVSVLREFAKDKNIKNISTMRKSELIEAIIAAAEGEEKNEPAEVASVVAEDEADGQNNHKTSEYS
HQKSEQYVKEEHKDNYHAQSHKGSEQSKQEQDMPMADGILEVLSEGYGFIRCENFLPGENDVYVSPSQIRKFNLKTGDII
QGPKRMKTSGEKFSALMYLESVNGKDPSVAQKRPAFESLTPIFPNERIHLEKNSSTVAMRMVDLISPIGKGQRGMIVSQP
KSGKTTLLKQIANAVTKNNPEMHLMILLIDERPEEVTDIKESIVGDNVEVIYSTFDELPERHKRVSEMVIERAKRLVEQK
TDVIILLDSITRLARAYNLTVQASGRTLSGGLDPAALHMPKRFFGAARNMREGGSLTILATALVETGSKMDDVVFEEFKG
TGNMELVLDRKLSEKRVFPAIDIAKSSTRREDLLLNKEEQEAVDIMRKALNGLKTDEAAERILDLFVKTKNNEELVRTVI
KNHYI

Sequences:

>Translated_485_residues
MNTEVDYMKAKLETLSVSVLREFAKDKNIKNISTMRKSELIEAIIAAAEGEEKNEPAEVASVVAEDEADGQNNHKTSEYS
HQKSEQYVKEEHKDNYHAQSHKGSEQSKQEQDMPMADGILEVLSEGYGFIRCENFLPGENDVYVSPSQIRKFNLKTGDII
QGPKRMKTSGEKFSALMYLESVNGKDPSVAQKRPAFESLTPIFPNERIHLEKNSSTVAMRMVDLISPIGKGQRGMIVSQP
KSGKTTLLKQIANAVTKNNPEMHLMILLIDERPEEVTDIKESIVGDNVEVIYSTFDELPERHKRVSEMVIERAKRLVEQK
TDVIILLDSITRLARAYNLTVQASGRTLSGGLDPAALHMPKRFFGAARNMREGGSLTILATALVETGSKMDDVVFEEFKG
TGNMELVLDRKLSEKRVFPAIDIAKSSTRREDLLLNKEEQEAVDIMRKALNGLKTDEAAERILDLFVKTKNNEELVRTVI
KNHYI
>Mature_485_residues
MNTEVDYMKAKLETLSVSVLREFAKDKNIKNISTMRKSELIEAIIAAAEGEEKNEPAEVASVVAEDEADGQNNHKTSEYS
HQKSEQYVKEEHKDNYHAQSHKGSEQSKQEQDMPMADGILEVLSEGYGFIRCENFLPGENDVYVSPSQIRKFNLKTGDII
QGPKRMKTSGEKFSALMYLESVNGKDPSVAQKRPAFESLTPIFPNERIHLEKNSSTVAMRMVDLISPIGKGQRGMIVSQP
KSGKTTLLKQIANAVTKNNPEMHLMILLIDERPEEVTDIKESIVGDNVEVIYSTFDELPERHKRVSEMVIERAKRLVEQK
TDVIILLDSITRLARAYNLTVQASGRTLSGGLDPAALHMPKRFFGAARNMREGGSLTILATALVETGSKMDDVVFEEFKG
TGNMELVLDRKLSEKRVFPAIDIAKSSTRREDLLLNKEEQEAVDIMRKALNGLKTDEAAERILDLFVKTKNNEELVRTVI
KNHYI

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=376, Percent_Identity=54.5212765957447, Blast_Score=406, Evalue=1e-114,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 54514; Mature: 54514

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTEVDYMKAKLETLSVSVLREFAKDKNIKNISTMRKSELIEAIIAAAEGEEKNEPAEVA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
SVVAEDEADGQNNHKTSEYSHQKSEQYVKEEHKDNYHAQSHKGSEQSKQEQDMPMADGIL
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCHHHHHH
EVLSEGYGFIRCENFLPGENDVYVSPSQIRKFNLKTGDIIQGPKRMKTSGEKFSALMYLE
HHHHCCCCEEEECCCCCCCCCEEECHHHHHEECCCCCCCCCCCHHHHCCCHHHHHHHEEH
SVNGKDPSVAQKRPAFESLTPIFPNERIHLEKNSSTVAMRMVDLISPIGKGQRGMIVSQP
HCCCCCCCHHHHCCCHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEECC
KSGKTTLLKQIANAVTKNNPEMHLMILLIDERPEEVTDIKESIVGDNVEVIYSTFDELPE
CCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
RHKRVSEMVIERAKRLVEQKTDVIILLDSITRLARAYNLTVQASGRTLSGGLDPAALHMP
HHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCEEEEECCCEECCCCCHHHHHCC
KRFFGAARNMREGGSLTILATALVETGSKMDDVVFEEFKGTGNMELVLDRKLSEKRVFPA
HHHHHHHHCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCH
IDIAKSSTRREDLLLNKEEQEAVDIMRKALNGLKTDEAAERILDLFVKTKNNEELVRTVI
HHHHHCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
KNHYI
HHCCC
>Mature Secondary Structure
MNTEVDYMKAKLETLSVSVLREFAKDKNIKNISTMRKSELIEAIIAAAEGEEKNEPAEVA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
SVVAEDEADGQNNHKTSEYSHQKSEQYVKEEHKDNYHAQSHKGSEQSKQEQDMPMADGIL
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCHHHHHH
EVLSEGYGFIRCENFLPGENDVYVSPSQIRKFNLKTGDIIQGPKRMKTSGEKFSALMYLE
HHHHCCCCEEEECCCCCCCCCEEECHHHHHEECCCCCCCCCCCHHHHCCCHHHHHHHEEH
SVNGKDPSVAQKRPAFESLTPIFPNERIHLEKNSSTVAMRMVDLISPIGKGQRGMIVSQP
HCCCCCCCHHHHCCCHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEECC
KSGKTTLLKQIANAVTKNNPEMHLMILLIDERPEEVTDIKESIVGDNVEVIYSTFDELPE
CCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
RHKRVSEMVIERAKRLVEQKTDVIILLDSITRLARAYNLTVQASGRTLSGGLDPAALHMP
HHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCEEEEECCCEECCCCCHHHHHCC
KRFFGAARNMREGGSLTILATALVETGSKMDDVVFEEFKGTGNMELVLDRKLSEKRVFPA
HHHHHHHHCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCH
IDIAKSSTRREDLLLNKEEQEAVDIMRKALNGLKTDEAAERILDLFVKTKNNEELVRTVI
HHHHHCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
KNHYI
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423140; 9353933; 9384377 [H]