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The map label for this gene is tcyB [H]

Identifier: 238917322

GI number: 238917322

Start: 1442166

End: 1443698

Strand: Reverse

Name: tcyB [H]

Synonym: EUBELI_01397

Alternate gene names: 238917322

Gene position: 1443698-1442166 (Counterclockwise)

Preceding gene: 238917323

Following gene: 238917321

Centisome position: 67.33

GC content: 38.75

Gene sequence:

>1533_bases
ATGGATTTTTATATGGCTAAGAATATCAGAAAAGCCGTCCTGTTACTGGCAATGTTGTCTGTGATTGTGGGAATGTTCAC
AGCATGCAGTAATAAAAAGAGCGATGATGGACGAACTACATTTACAGTAGGATTTGATGCGGAATTCCCACCTTATGGTT
ACAAGGATGATAGTGGAGAGTATGTAGGTTTTGACCTTGATCTTGCACAGGAAGTATGTGAAAGAAATGGCTGGAATCTG
GTTAAGCAGCCTATTGACTGGGATTCTAAAGATATGGAGCTTAGTTCAGGCTCTATTGACTGTATATGGAACGGTTTCAC
GCTTAATGGAAGAGAGCGTGAGTATACATGGTCTAAGGCATATATTGATAATTCACAGGTTGTTATAGTTAAATCTGGTT
TAGGAATAAAGAAGTTAGAAGACCTTAAGGGTAAGGTTGTTATTGTACAGGCTGATTCATCAGCTCTTGCAGCATTTACA
GGAGAGGATGCAACAGAAGAAAATCTTGCACTTGCGGCACAATTTAAGACACTTCAGCAGGTATCTGATTATAATTCAGC
ATTTATGAACCTTGAATCAGGTTCGGCAGATGCTGTATGTATGGATATGGGTGTTGCAAAGTACCAGCTTGAGCAGAGAG
GTAACAAGTTCACAATGCTTGATGAGACTGTTTCATCCGAACAGTATGGTATTGGTTTTAAGTTAGGTAATACAGCATTA
AGAGATGAAGTTCAGACAAGCCTTAATGATATGCTTGCTGATGGCACATTTGACAAGATTGCTGAAAAATGGGGACTTAC
AGACAGCGTATGTCTTGGAGAAGAAGGAACAGACAGTGCCTATCTCCTTGACAGTACCAGTACAACTAAGGATTCATTTG
GTGAAAGACTTGTGGATATAGTTAAGCAGCTTTCATCAGGAATGTTAGCAACTCTTGCAATATTCTTCTTAACACTTATA
TTCTCAATGCCGCTAGGACTTCTTGTATGTGAAATAAGAAAGTCAAGAATAGGCATTGTAAGATGGATTGCGAGAATATA
CATATCTATAATGAGAGGAACTCCTCTTATGTTACAGCTTCTTGTTGTGTTCTTCGGACCATATTATCTGTTTGGAATGA
AGGTTACGGCAACATACAGATTCTATGCTGTAATTATTGGATTTGCACTTAATTATGCAGCATATTTTGCAGAAATATAC
AGAGGTGGAATTGAGAGCATACCTAAGGGACAGTACGAGGCAGCAGAGGTACTCGGATATTCCAAGGTACAGACATTCTG
GAAGATTATATTCCCTCAGATGGTTAAGAGAGTACTTCCTCCGGTTACTAACGAGGTTATAACACTTGTTAAGGATACAT
CACTTGCATTTGCACTTGCTTACACAGAAATGTTTACACTTGCCAAGCAGGTAGCGGCTTCAACAGCAAGTATCATGCCA
TTATTCATAGCAGGATTGTTCTATTACATTTTCAATTTCCTTGTTGCTATGGTTATGGAAAAGCTAGAAAAGCGTATGCA
GTATTTTGATTAA

Upstream 100 bases:

>100_bases
TATTTAGAATATTTATAGTGCTTTTATCAGGTAATTCATGTTATAATAGACAAGCTTGATTTACTGTGGTAAAGTGAAAG
CATATTAAAGCGAGAAAGGC

Downstream 100 bases:

>100_bases
GGAGGATTTTAATATGAAGAAATTATTAGAAATATCACATTTAAAGAAATCCTTCGGCGATACTGAGGTGCTTAAGGATA
TATCAATGGAAGTTAATGAG

Product: polar amino acid transport system substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MDFYMAKNIRKAVLLLAMLSVIVGMFTACSNKKSDDGRTTFTVGFDAEFPPYGYKDDSGEYVGFDLDLAQEVCERNGWNL
VKQPIDWDSKDMELSSGSIDCIWNGFTLNGREREYTWSKAYIDNSQVVIVKSGLGIKKLEDLKGKVVIVQADSSALAAFT
GEDATEENLALAAQFKTLQQVSDYNSAFMNLESGSADAVCMDMGVAKYQLEQRGNKFTMLDETVSSEQYGIGFKLGNTAL
RDEVQTSLNDMLADGTFDKIAEKWGLTDSVCLGEEGTDSAYLLDSTSTTKDSFGERLVDIVKQLSSGMLATLAIFFLTLI
FSMPLGLLVCEIRKSRIGIVRWIARIYISIMRGTPLMLQLLVVFFGPYYLFGMKVTATYRFYAVIIGFALNYAAYFAEIY
RGGIESIPKGQYEAAEVLGYSKVQTFWKIIFPQMVKRVLPPVTNEVITLVKDTSLAFALAYTEMFTLAKQVAASTASIMP
LFIAGLFYYIFNFLVAMVMEKLEKRMQYFD

Sequences:

>Translated_510_residues
MDFYMAKNIRKAVLLLAMLSVIVGMFTACSNKKSDDGRTTFTVGFDAEFPPYGYKDDSGEYVGFDLDLAQEVCERNGWNL
VKQPIDWDSKDMELSSGSIDCIWNGFTLNGREREYTWSKAYIDNSQVVIVKSGLGIKKLEDLKGKVVIVQADSSALAAFT
GEDATEENLALAAQFKTLQQVSDYNSAFMNLESGSADAVCMDMGVAKYQLEQRGNKFTMLDETVSSEQYGIGFKLGNTAL
RDEVQTSLNDMLADGTFDKIAEKWGLTDSVCLGEEGTDSAYLLDSTSTTKDSFGERLVDIVKQLSSGMLATLAIFFLTLI
FSMPLGLLVCEIRKSRIGIVRWIARIYISIMRGTPLMLQLLVVFFGPYYLFGMKVTATYRFYAVIIGFALNYAAYFAEIY
RGGIESIPKGQYEAAEVLGYSKVQTFWKIIFPQMVKRVLPPVTNEVITLVKDTSLAFALAYTEMFTLAKQVAASTASIMP
LFIAGLFYYIFNFLVAMVMEKLEKRMQYFD
>Mature_510_residues
MDFYMAKNIRKAVLLLAMLSVIVGMFTACSNKKSDDGRTTFTVGFDAEFPPYGYKDDSGEYVGFDLDLAQEVCERNGWNL
VKQPIDWDSKDMELSSGSIDCIWNGFTLNGREREYTWSKAYIDNSQVVIVKSGLGIKKLEDLKGKVVIVQADSSALAAFT
GEDATEENLALAAQFKTLQQVSDYNSAFMNLESGSADAVCMDMGVAKYQLEQRGNKFTMLDETVSSEQYGIGFKLGNTAL
RDEVQTSLNDMLADGTFDKIAEKWGLTDSVCLGEEGTDSAYLLDSTSTTKDSFGERLVDIVKQLSSGMLATLAIFFLTLI
FSMPLGLLVCEIRKSRIGIVRWIARIYISIMRGTPLMLQLLVVFFGPYYLFGMKVTATYRFYAVIIGFALNYAAYFAEIY
RGGIESIPKGQYEAAEVLGYSKVQTFWKIIFPQMVKRVLPPVTNEVITLVKDTSLAFALAYTEMFTLAKQVAASTASIMP
LFIAGLFYYIFNFLVAMVMEKLEKRMQYFD

Specific function: Part of the ABC transporter complex tcyABC involved in L-cystine import. Probably responsible for the translocation of the substrate across the membrane (Probable) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788226, Length=189, Percent_Identity=38.0952380952381, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87082239, Length=207, Percent_Identity=35.7487922705314, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787086, Length=222, Percent_Identity=28.8288288288288, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1787030, Length=209, Percent_Identity=30.622009569378, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787031, Length=252, Percent_Identity=27.7777777777778, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1788228, Length=256, Percent_Identity=28.125, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788645, Length=207, Percent_Identity=31.4009661835749, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1786873, Length=215, Percent_Identity=29.3023255813954, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1787087, Length=227, Percent_Identity=25.9911894273128, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787085, Length=229, Percent_Identity=27.9475982532751, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1788649, Length=265, Percent_Identity=26.4150943396226, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788646, Length=209, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI48994930, Length=126, Percent_Identity=31.7460317460317, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787088, Length=225, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788648, Length=235, Percent_Identity=28.5106382978723, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786874, Length=212, Percent_Identity=23.1132075471698, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 56985; Mature: 56985

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50928 ABC_TM1 ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFYMAKNIRKAVLLLAMLSVIVGMFTACSNKKSDDGRTTFTVGFDAEFPPYGYKDDSGE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCC
YVGFDLDLAQEVCERNGWNLVKQPIDWDSKDMELSSGSIDCIWNGFTLNGREREYTWSKA
EEEECHHHHHHHHHCCCCEEECCCCCCCCCCCEECCCCEEEEEECEEECCCCCCEEEHHE
YIDNSQVVIVKSGLGIKKLEDLKGKVVIVQADSSALAAFTGEDATEENLALAAQFKTLQQ
EECCCEEEEEECCCCHHHHHHCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHH
VSDYNSAFMNLESGSADAVCMDMGVAKYQLEQRGNKFTMLDETVSSEQYGIGFKLGNTAL
HHHHHHHHEEECCCCCCEEHHHCCHHHHHHHHCCCEEEEEHHHHCCCCCCCEEEECCHHH
RDEVQTSLNDMLADGTFDKIAEKWGLTDSVCLGEEGTDSAYLLDSTSTTKDSFGERLVDI
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCEECCCCCCCCEEEEECCCCCHHHHHHHHHHH
VKQLSSGMLATLAIFFLTLIFSMPLGLLVCEIRKSRIGIVRWIARIYISIMRGTPLMLQL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LVVFFGPYYLFGMKVTATYRFYAVIIGFALNYAAYFAEIYRGGIESIPKGQYEAAEVLGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHH
SKVQTFWKIIFPQMVKRVLPPVTNEVITLVKDTSLAFALAYTEMFTLAKQVAASTASIMP
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFIAGLFYYIFNFLVAMVMEKLEKRMQYFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDFYMAKNIRKAVLLLAMLSVIVGMFTACSNKKSDDGRTTFTVGFDAEFPPYGYKDDSGE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCC
YVGFDLDLAQEVCERNGWNLVKQPIDWDSKDMELSSGSIDCIWNGFTLNGREREYTWSKA
EEEECHHHHHHHHHCCCCEEECCCCCCCCCCCEECCCCEEEEEECEEECCCCCCEEEHHE
YIDNSQVVIVKSGLGIKKLEDLKGKVVIVQADSSALAAFTGEDATEENLALAAQFKTLQQ
EECCCEEEEEECCCCHHHHHHCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHH
VSDYNSAFMNLESGSADAVCMDMGVAKYQLEQRGNKFTMLDETVSSEQYGIGFKLGNTAL
HHHHHHHHEEECCCCCCEEHHHCCHHHHHHHHCCCEEEEEHHHHCCCCCCCEEEECCHHH
RDEVQTSLNDMLADGTFDKIAEKWGLTDSVCLGEEGTDSAYLLDSTSTTKDSFGERLVDI
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCEECCCCCCCCEEEEECCCCCHHHHHHHHHHH
VKQLSSGMLATLAIFFLTLIFSMPLGLLVCEIRKSRIGIVRWIARIYISIMRGTPLMLQL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LVVFFGPYYLFGMKVTATYRFYAVIIGFALNYAAYFAEIYRGGIESIPKGQYEAAEVLGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHH
SKVQTFWKIIFPQMVKRVLPPVTNEVITLVKDTSLAFALAYTEMFTLAKQVAASTASIMP
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFIAGLFYYIFNFLVAMVMEKLEKRMQYFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7551042; 8969502; 9384377 [H]