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The map label for this gene is metH [H]

Identifier: 238917307

GI number: 238917307

Start: 1422007

End: 1424412

Strand: Reverse

Name: metH [H]

Synonym: EUBELI_01382

Alternate gene names: 238917307

Gene position: 1424412-1422007 (Counterclockwise)

Preceding gene: 238917308

Following gene: 238917306

Centisome position: 66.43

GC content: 39.98

Gene sequence:

>2406_bases
GTGGCTGACAGATTCAGAGAACTCTTAAAAAATAGAGATTATATAATATTTGATGGTGCTATGGGTACAATGCTTCAGGC
AGCTGGCATGAAGATGGGAGAGACACCTGAAGTGCTTAATATAACAAGGCCGGAGCTTCTTGTTTCAATTGCAGAGCAGT
ATTTTAATGCCGGTTCAGATGTGGTATATGCCAATACATTTGGTGCAAACAGATATAAGCTTGAAGAATGCGGAAAAAGC
GTTGAGGAGCTTGTAACTGCCGGAATTGTCAATGCAAAGAAGGCAAGAGATGCAGTAAAGCCTGACGGACTTGTTGCACT
CGATGTCGGACCAATCGGACAACTTCTTGAGCCAACAGGCGTATTAAGCTTTGAAGAAGCATATGATATGTATGCCGAGA
TAGTAAAGGCTGGAGCTGCTGCGGGAGCAGACCTTGTTGTATTTGAGACAATGACCGACCTTTTAGATGTTAAGGCTGCT
GTACTTGCAGCTAAGGAAAACAGCGACCTTCCTATAGCTGCTACGATGACATTTGAACAGAATATGAGAACATTCACAGG
CTGTTCTATATCAGCAATGGCTCTTACGCTTACAGGACTTGGCGTTGATGCACTCGGTGTAAACTGTTCTTTAGGACCTA
AGGAATTAGAACCGGTTATTGAAGAACTTGTAAAATGGACTAATCTTCCAATAATAGTAAAGCCTAATGCAGGACTTCCT
GACCCTGAAACTAACCTTTATAATGTTACTGCAGTCCAGTTTGCAGATTTCATGAAGGATTTAAGAAAATATGGCATTAA
GATATTCGGCGGCTGCTGTGGAACTAATCCTGAATTCATTAAAGAATTATCAGAAATGTTAAAAAGAGAAGGCAATCCGG
CAGCACCACACAAATATATTCCGGGTGCAGTGTGTTCAGCAACAAGCACTGTTGTCGTTGATGAGCCAAGAATTATCGGT
GAAAGAATCAATCCTACAGGTAAGAAGCTTTTTAAGGAAGCACTTTTAAGACATGATATGGATTATATTCTTGGACAGGC
TTTAGAGCAGATATCAGGCGGAGCAGATATTCTTGATGTCAATGTAGGTCTTCCGGGAATTGATGAGCGTGAAATGATGA
TTGATACAATAAAGTCATTACAGGCAGTTGTAGATGTCCCACTTCAGATCGATTCTACAATACCTGAAGTACTTGAGGCA
GCACTCCGTGTATACAATGGTAAGCCGCTTGTTAACTCAGTTAATGGTGAGGAGGAGTCTCTTAACAATGTTCTTCCTCT
TGTTAAGAAATATGGTGCAGGAGTTATCGGACTTGCACTTGATAAAGATGGCATTCCTAAGAAGGCAGAGGACAGAGTTG
CAATAGCAAAGAAGATTATGGACAGGGCTGTTGCAATGGGAATCCCTAAAGAAGATATTTATATTGACTGCCTTACACTC
ACAGCTTCTGCTGAACAGGAGGGCGTAATGGAAACTCTTAATGCACTTCATACAGTTAAGAATGAGCTTGGCTTAAAGAC
AGTGCTTGGTGTTTCTAATATTTCATTCGGACTTCCAAACAGAGTGCTTGTAAACCACATTTTCCTTACAATGGCACTTA
CTAACGGACTTGACCTTGCGATTATAAATCCTAACATCCCAGAGATGACAGGTGCGGTAAGGGCGTACAAGCTTCTTGCC
AATATTGATAAGAATTCAGTTGATTATATAAAAAACTATGGCGCTATGCCAAATGTTTCTAAGATTGATCCTGTCAAGAA
GGAAAAGAAGGATGGGAACTATACAGGAGATGACCTTTTCTATGCCGTAGAAAAAGGACTTAAAAATGAAGGGGCTGAGA
TAACAGAGGCACTTCTTAAAACTATGGATTCGATGGAGATTGTTAATCAGGTGCTTATTCCTGCGCTTGATAAGATAGGT
GCTGAATTCGAGAAGGGTACATTGTTCCTTCCACAGCTTATTATGGCAGCATCAGTTACACAGGCAGCATTTGAAGTTAT
AAGAAAGCATATGGTTATGTCTGATAATGCTCCTGTAAGCAAGGGAAAGATTGTTATAGCTACAGTCAAGGGGGATGTCC
ATGATATCGGTAAGAATATTGTTAAGGTTCTTCTTGAAAATTATGGATATGATGTTATTGACCTTGGTAAAGATGTCGAG
TACCAGACAGTTGTTGATGCAATAAGAGATAATGATGTTAAACTGTGTGGACTTTCTGCACTTATGACAACAACACTTGT
ATCTATGAAGGAAACAATTGCACTTATAAGAGAAAATAATCTTGATTGTAAGGTTATGGTTGGCGGAGCAGTACTTACTC
CGGAATATGCTAAAGAGATAGATGCAGATTTCTATGCAAAGGATGCAAAAGAATCTGTTGATATTGCTAAAAGAGTTCTT
GGTTAA

Upstream 100 bases:

>100_bases
ATCGGACTTACTAAGGAGCCGGTAAGTACTGAGAACAGAGGTTGCGCAACCTGCAATCTTAATAAAACCTGCGCATACAG
AGGAATGGGAGGACATTGTA

Downstream 100 bases:

>100_bases
AATTTTGACATATCGAGAAAAAATGTTTATAATTATAGCACTTAGTATAATTGATTTATATAATATTTGATGTGTTTTTT
ATATATGCAATTGTAGTCTG

Product: 5-methyltetrahydrofolate--homocysteine methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 801; Mature: 800

Protein sequence:

>801_residues
MADRFRELLKNRDYIIFDGAMGTMLQAAGMKMGETPEVLNITRPELLVSIAEQYFNAGSDVVYANTFGANRYKLEECGKS
VEELVTAGIVNAKKARDAVKPDGLVALDVGPIGQLLEPTGVLSFEEAYDMYAEIVKAGAAAGADLVVFETMTDLLDVKAA
VLAAKENSDLPIAATMTFEQNMRTFTGCSISAMALTLTGLGVDALGVNCSLGPKELEPVIEELVKWTNLPIIVKPNAGLP
DPETNLYNVTAVQFADFMKDLRKYGIKIFGGCCGTNPEFIKELSEMLKREGNPAAPHKYIPGAVCSATSTVVVDEPRIIG
ERINPTGKKLFKEALLRHDMDYILGQALEQISGGADILDVNVGLPGIDEREMMIDTIKSLQAVVDVPLQIDSTIPEVLEA
ALRVYNGKPLVNSVNGEEESLNNVLPLVKKYGAGVIGLALDKDGIPKKAEDRVAIAKKIMDRAVAMGIPKEDIYIDCLTL
TASAEQEGVMETLNALHTVKNELGLKTVLGVSNISFGLPNRVLVNHIFLTMALTNGLDLAIINPNIPEMTGAVRAYKLLA
NIDKNSVDYIKNYGAMPNVSKIDPVKKEKKDGNYTGDDLFYAVEKGLKNEGAEITEALLKTMDSMEIVNQVLIPALDKIG
AEFEKGTLFLPQLIMAASVTQAAFEVIRKHMVMSDNAPVSKGKIVIATVKGDVHDIGKNIVKVLLENYGYDVIDLGKDVE
YQTVVDAIRDNDVKLCGLSALMTTTLVSMKETIALIRENNLDCKVMVGGAVLTPEYAKEIDADFYAKDAKESVDIAKRVL
G

Sequences:

>Translated_801_residues
MADRFRELLKNRDYIIFDGAMGTMLQAAGMKMGETPEVLNITRPELLVSIAEQYFNAGSDVVYANTFGANRYKLEECGKS
VEELVTAGIVNAKKARDAVKPDGLVALDVGPIGQLLEPTGVLSFEEAYDMYAEIVKAGAAAGADLVVFETMTDLLDVKAA
VLAAKENSDLPIAATMTFEQNMRTFTGCSISAMALTLTGLGVDALGVNCSLGPKELEPVIEELVKWTNLPIIVKPNAGLP
DPETNLYNVTAVQFADFMKDLRKYGIKIFGGCCGTNPEFIKELSEMLKREGNPAAPHKYIPGAVCSATSTVVVDEPRIIG
ERINPTGKKLFKEALLRHDMDYILGQALEQISGGADILDVNVGLPGIDEREMMIDTIKSLQAVVDVPLQIDSTIPEVLEA
ALRVYNGKPLVNSVNGEEESLNNVLPLVKKYGAGVIGLALDKDGIPKKAEDRVAIAKKIMDRAVAMGIPKEDIYIDCLTL
TASAEQEGVMETLNALHTVKNELGLKTVLGVSNISFGLPNRVLVNHIFLTMALTNGLDLAIINPNIPEMTGAVRAYKLLA
NIDKNSVDYIKNYGAMPNVSKIDPVKKEKKDGNYTGDDLFYAVEKGLKNEGAEITEALLKTMDSMEIVNQVLIPALDKIG
AEFEKGTLFLPQLIMAASVTQAAFEVIRKHMVMSDNAPVSKGKIVIATVKGDVHDIGKNIVKVLLENYGYDVIDLGKDVE
YQTVVDAIRDNDVKLCGLSALMTTTLVSMKETIALIRENNLDCKVMVGGAVLTPEYAKEIDADFYAKDAKESVDIAKRVL
G
>Mature_800_residues
ADRFRELLKNRDYIIFDGAMGTMLQAAGMKMGETPEVLNITRPELLVSIAEQYFNAGSDVVYANTFGANRYKLEECGKSV
EELVTAGIVNAKKARDAVKPDGLVALDVGPIGQLLEPTGVLSFEEAYDMYAEIVKAGAAAGADLVVFETMTDLLDVKAAV
LAAKENSDLPIAATMTFEQNMRTFTGCSISAMALTLTGLGVDALGVNCSLGPKELEPVIEELVKWTNLPIIVKPNAGLPD
PETNLYNVTAVQFADFMKDLRKYGIKIFGGCCGTNPEFIKELSEMLKREGNPAAPHKYIPGAVCSATSTVVVDEPRIIGE
RINPTGKKLFKEALLRHDMDYILGQALEQISGGADILDVNVGLPGIDEREMMIDTIKSLQAVVDVPLQIDSTIPEVLEAA
LRVYNGKPLVNSVNGEEESLNNVLPLVKKYGAGVIGLALDKDGIPKKAEDRVAIAKKIMDRAVAMGIPKEDIYIDCLTLT
ASAEQEGVMETLNALHTVKNELGLKTVLGVSNISFGLPNRVLVNHIFLTMALTNGLDLAIINPNIPEMTGAVRAYKLLAN
IDKNSVDYIKNYGAMPNVSKIDPVKKEKKDGNYTGDDLFYAVEKGLKNEGAEITEALLKTMDSMEIVNQVLIPALDKIGA
EFEKGTLFLPQLIMAASVTQAAFEVIRKHMVMSDNAPVSKGKIVIATVKGDVHDIGKNIVKVLLENYGYDVIDLGKDVEY
QTVVDAIRDNDVKLCGLSALMTTTLVSMKETIALIRENNLDCKVMVGGAVLTPEYAKEIDADFYAKDAKESVDIAKRVLG

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=894, Percent_Identity=27.5167785234899, Blast_Score=249, Evalue=8e-66,
Organism=Escherichia coli, GI1790450, Length=869, Percent_Identity=28.078250863061, Blast_Score=274, Evalue=2e-74,
Organism=Escherichia coli, GI1786456, Length=304, Percent_Identity=26.9736842105263, Blast_Score=81, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17535405, Length=890, Percent_Identity=27.1910112359551, Blast_Score=241, Evalue=1e-63,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 86706; Mature: 86574

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS50970 HCY ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRFRELLKNRDYIIFDGAMGTMLQAAGMKMGETPEVLNITRPELLVSIAEQYFNAGSD
CCHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
VVYANTFGANRYKLEECGKSVEELVTAGIVNAKKARDAVKPDGLVALDVGPIGQLLEPTG
EEEEECCCCCCEEHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCEEEEECCCCHHHCCCCC
VLSFEEAYDMYAEIVKAGAAAGADLVVFETMTDLLDVKAAVLAAKENSDLPIAATMTFEQ
CCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHEECCCCCCCEEEEEEHHH
NMRTFTGCSISAMALTLTGLGVDALGVNCSLGPKELEPVIEELVKWTNLPIIVKPNAGLP
CCHHHCCCCHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCC
DPETNLYNVTAVQFADFMKDLRKYGIKIFGGCCGTNPEFIKELSEMLKREGNPAAPHKYI
CCCCCEEEEHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
PGAVCSATSTVVVDEPRIIGERINPTGKKLFKEALLRHDMDYILGQALEQISGGADILDV
CCHHHCCCCEEEECCCHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEE
NVGLPGIDEREMMIDTIKSLQAVVDVPLQIDSTIPEVLEAALRVYNGKPLVNSVNGEEES
ECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCCHHH
LNNVLPLVKKYGAGVIGLALDKDGIPKKAEDRVAIAKKIMDRAVAMGIPKEDIYIDCLTL
HHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
TASAEQEGVMETLNALHTVKNELGLKTVLGVSNISFGLPNRVLVNHIFLTMALTNGLDLA
ECCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEE
IINPNIPEMTGAVRAYKLLANIDKNSVDYIKNYGAMPNVSKIDPVKKEKKDGNYTGDDLF
EECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCHHHH
YAVEKGLKNEGAEITEALLKTMDSMEIVNQVLIPALDKIGAEFEKGTLFLPQLIMAASVT
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
QAAFEVIRKHMVMSDNAPVSKGKIVIATVKGDVHDIGKNIVKVLLENYGYDVIDLGKDVE
HHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCCCCH
YQTVVDAIRDNDVKLCGLSALMTTTLVSMKETIALIRENNLDCKVMVGGAVLTPEYAKEI
HHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEECCHHHHHC
DADFYAKDAKESVDIAKRVLG
CCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADRFRELLKNRDYIIFDGAMGTMLQAAGMKMGETPEVLNITRPELLVSIAEQYFNAGSD
CHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
VVYANTFGANRYKLEECGKSVEELVTAGIVNAKKARDAVKPDGLVALDVGPIGQLLEPTG
EEEEECCCCCCEEHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCEEEEECCCCHHHCCCCC
VLSFEEAYDMYAEIVKAGAAAGADLVVFETMTDLLDVKAAVLAAKENSDLPIAATMTFEQ
CCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHEECCCCCCCEEEEEEHHH
NMRTFTGCSISAMALTLTGLGVDALGVNCSLGPKELEPVIEELVKWTNLPIIVKPNAGLP
CCHHHCCCCHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCC
DPETNLYNVTAVQFADFMKDLRKYGIKIFGGCCGTNPEFIKELSEMLKREGNPAAPHKYI
CCCCCEEEEHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
PGAVCSATSTVVVDEPRIIGERINPTGKKLFKEALLRHDMDYILGQALEQISGGADILDV
CCHHHCCCCEEEECCCHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEE
NVGLPGIDEREMMIDTIKSLQAVVDVPLQIDSTIPEVLEAALRVYNGKPLVNSVNGEEES
ECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCCHHH
LNNVLPLVKKYGAGVIGLALDKDGIPKKAEDRVAIAKKIMDRAVAMGIPKEDIYIDCLTL
HHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
TASAEQEGVMETLNALHTVKNELGLKTVLGVSNISFGLPNRVLVNHIFLTMALTNGLDLA
ECCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEE
IINPNIPEMTGAVRAYKLLANIDKNSVDYIKNYGAMPNVSKIDPVKKEKKDGNYTGDDLF
EECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCHHHH
YAVEKGLKNEGAEITEALLKTMDSMEIVNQVLIPALDKIGAEFEKGTLFLPQLIMAASVT
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
QAAFEVIRKHMVMSDNAPVSKGKIVIATVKGDVHDIGKNIVKVLLENYGYDVIDLGKDVE
HHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCCCCH
YQTVVDAIRDNDVKLCGLSALMTTTLVSMKETIALIRENNLDCKVMVGGAVLTPEYAKEI
HHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEECCHHHHHC
DADFYAKDAKESVDIAKRVLG
CCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]