Click here to switch to the map view.

The map label for this gene is grpE [H]

Identifier: 238917286

GI number: 238917286

Start: 1400632

End: 1401270

Strand: Reverse

Name: grpE [H]

Synonym: EUBELI_01361

Alternate gene names: 238917286

Gene position: 1401270-1400632 (Counterclockwise)

Preceding gene: 238917287

Following gene: 238917285

Centisome position: 65.35

GC content: 41.0

Gene sequence:

>639_bases
GTGTCAAAGGATGTAAAGAAAAAGAAATCAGAAGAGACAGTTCAGGATGAAGTAAAGAATCAGGAGACTGATGCTAAGAA
AGAAGAAGCCTGCAACTGCGAATCAGAATGTAAGGCTGATGAAAGTCAGAATACATGTGAAGCTGATGAATCAGACAAGG
CCGAGGCACAGGATGAGAGCACTGAGAGCTCTAAGAAGAAAGATCCTAAGGATGCAGTTATAGAAGAACTTCAGGACAGA
GTAAAGAGGCAGATGGCTGAGTTTGATAATTTCAGAAAGAGAACAGAGAAAGAGAAATCTACTATGTTCGAGATGGGAGC
CTCAGATATCATTAAGAAGCTTTTACCTATTGTTGATAATTTCGACAGAGGATTTAAGAGTGTGACAGATGAGGAACTTG
AAACTCCATTCGCTAAGGGAATGGATATGGTTCACAAGCAGCTTTTAAAGATGCTTGAGGATGCAGATGTTAAGCCTATT
GAAGCTTTAGGCGGTGAGTTCAATCCAGATTTCCACAATGCAGTAATGCATGTCGAGGATGATTCAGTTGGAGAGAACAT
CGTAGTTGAAGAGTTTGAGAAGGGCTACACATACAGAGACCAGGTTATAAGACACAGCATGGTCAAGGTTGCTAACTAG

Upstream 100 bases:

>100_bases
CGAAGCGTATGGATTATGAAAAGGTTGTTGATACCTTAAAAGAGATGCAGACGCATTTGGATGATGTATTTAAGAAAACT
TAGGAAAGGTTGGTATTGAA

Downstream 100 bases:

>100_bases
CGATAAGCCAGAAAGCATATGTAACACAAGGTATTAATCAATTTAATTATAGAAAGGACATTTTATCATGGGAAAGATTA
TTGGTATTGATTTAGGTACA

Product: molecular chaperone GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MSKDVKKKKSEETVQDEVKNQETDAKKEEACNCESECKADESQNTCEADESDKAEAQDESTESSKKKDPKDAVIEELQDR
VKRQMAEFDNFRKRTEKEKSTMFEMGASDIIKKLLPIVDNFDRGFKSVTDEELETPFAKGMDMVHKQLLKMLEDADVKPI
EALGGEFNPDFHNAVMHVEDDSVGENIVVEEFEKGYTYRDQVIRHSMVKVAN

Sequences:

>Translated_212_residues
MSKDVKKKKSEETVQDEVKNQETDAKKEEACNCESECKADESQNTCEADESDKAEAQDESTESSKKKDPKDAVIEELQDR
VKRQMAEFDNFRKRTEKEKSTMFEMGASDIIKKLLPIVDNFDRGFKSVTDEELETPFAKGMDMVHKQLLKMLEDADVKPI
EALGGEFNPDFHNAVMHVEDDSVGENIVVEEFEKGYTYRDQVIRHSMVKVAN
>Mature_211_residues
SKDVKKKKSEETVQDEVKNQETDAKKEEACNCESECKADESQNTCEADESDKAEAQDESTESSKKKDPKDAVIEELQDRV
KRQMAEFDNFRKRTEKEKSTMFEMGASDIIKKLLPIVDNFDRGFKSVTDEELETPFAKGMDMVHKQLLKMLEDADVKPIE
ALGGEFNPDFHNAVMHVEDDSVGENIVVEEFEKGYTYRDQVIRHSMVKVAN

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Escherichia coli, GI1788967, Length=166, Percent_Identity=29.5180722891566, Blast_Score=70, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24653432, Length=141, Percent_Identity=28.3687943262411, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 24256; Mature: 24125

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDVKKKKSEETVQDEVKNQETDAKKEEACNCESECKADESQNTCEADESDKAEAQDES
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHH
TESSKKKDPKDAVIEELQDRVKRQMAEFDNFRKRTEKEKSTMFEMGASDIIKKLLPIVDN
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
FDRGFKSVTDEELETPFAKGMDMVHKQLLKMLEDADVKPIEALGGEFNPDFHNAVMHVED
HHHHHHHHCHHHHHCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHEEECCC
DSVGENIVVEEFEKGYTYRDQVIRHSMVKVAN
CCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKDVKKKKSEETVQDEVKNQETDAKKEEACNCESECKADESQNTCEADESDKAEAQDES
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHH
TESSKKKDPKDAVIEELQDRVKRQMAEFDNFRKRTEKEKSTMFEMGASDIIKKLLPIVDN
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
FDRGFKSVTDEELETPFAKGMDMVHKQLLKMLEDADVKPIEALGGEFNPDFHNAVMHVED
HHHHHHHHCHHHHHCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHEEECCC
DSVGENIVVEEFEKGYTYRDQVIRHSMVKVAN
CCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA