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The map label for this gene is queA [H]

Identifier: 238917266

GI number: 238917266

Start: 1372580

End: 1373611

Strand: Reverse

Name: queA [H]

Synonym: EUBELI_01341

Alternate gene names: 238917266

Gene position: 1373611-1372580 (Counterclockwise)

Preceding gene: 238917267

Following gene: 238917265

Centisome position: 64.06

GC content: 39.53

Gene sequence:

>1032_bases
ATGGATTTACATGATTTTTATTATGATCTGCCAGAGGAGCTTATTGCACAGGATCCATTGTCGGACAGGTCAAGTTCAAG
ACTTATGGTGCTTGATAAGGAAACAGGAGAGATTGAACATAAGATATTCAGAGATATCGTAGATTATCTTAAGCCGGGTG
ATTGTCTTGTTGTTAATGACACTAAGGTAATTCCAGCGAGACTTATAGGTACTAAGGCTGATACAGGAGCTACTATTGAA
GTGCTTCTTCTTAAAAGACTTGAAGACAGACAGGATACATGGGAAGTTCTTGTTAAGCCGGGAAAGAAGGCTAAGGTTGG
AGCTAAGATAAGTTTTGGCGAAGGCAAGCTTATAGGAGAGATTATTGATATAGTTGAAGAAGGAAACAGACTTATCCAGT
TCACATATGACGGAATATTTGAAGAGATTCTTGATGAACTTGGACAGATGCCACTTCCACCATATATAACACATAAGCTT
GAGGATAAGAACAGATATCAGACTGTGTATGCAAAGCATGAGGGTTCTGCGGCGGCTCCTACTGCCGGTCTTCATTTTAC
AACAGAGCTACTTGAAAAAATCAAAGCTATGGGAGTTAACATTGCAAGAGTAACACTTCATGTGGGACTTGGAACATTCA
GACCTGTCAAGGTAGAGAATATTCTTGACCATCATATGCATTCGGAGTTTTATGTTGTTACACAGGAAGCAGCAGACATG
ATTAATAATACAAGAAAGAATGGTGGAAGGGTAATTGCTGTCGGAACAACAAGTACGAGAACACTTGAATCTGTTGCGCT
TGAAGATGGAACAATTCCAGCCAAGAGTGGATGGACAGAGATATTCATATATCCGGGATATAAGTTCAAGGCAATCGATT
GTCTTATAACTAATTTCCATCTTCCAGAATCAACACTTGTTATGCTTGTGTCGGCGCTTGCTGGAAGAGAACATGTACTT
CATGCATATGAAGTTGCGGTGCAGGAGAAATACAGATTCTTCAGCTTTGGAGATGCTATGTTTATTAAATAA

Upstream 100 bases:

>100_bases
AAAATCGGGAAGTTGTAGACGCGGATTGGACTTTAAAGCGGAGTGAGTCATGTAGGAAGTCCAATCCAGTGAAGTATAAA
GTACGGAAAGGAGTATAAGC

Downstream 100 bases:

>100_bases
TAATATATATTTATAGAAAAGACTTGCACATAAAAATAAAAAATGATATATTAAATGAGTAAAATGACTCATGAACACAG
ACGAACCCCCTCGGTGCCGT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MDLHDFYYDLPEELIAQDPLSDRSSSRLMVLDKETGEIEHKIFRDIVDYLKPGDCLVVNDTKVIPARLIGTKADTGATIE
VLLLKRLEDRQDTWEVLVKPGKKAKVGAKISFGEGKLIGEIIDIVEEGNRLIQFTYDGIFEEILDELGQMPLPPYITHKL
EDKNRYQTVYAKHEGSAAAPTAGLHFTTELLEKIKAMGVNIARVTLHVGLGTFRPVKVENILDHHMHSEFYVVTQEAADM
INNTRKNGGRVIAVGTTSTRTLESVALEDGTIPAKSGWTEIFIYPGYKFKAIDCLITNFHLPESTLVMLVSALAGREHVL
HAYEVAVQEKYRFFSFGDAMFIK

Sequences:

>Translated_343_residues
MDLHDFYYDLPEELIAQDPLSDRSSSRLMVLDKETGEIEHKIFRDIVDYLKPGDCLVVNDTKVIPARLIGTKADTGATIE
VLLLKRLEDRQDTWEVLVKPGKKAKVGAKISFGEGKLIGEIIDIVEEGNRLIQFTYDGIFEEILDELGQMPLPPYITHKL
EDKNRYQTVYAKHEGSAAAPTAGLHFTTELLEKIKAMGVNIARVTLHVGLGTFRPVKVENILDHHMHSEFYVVTQEAADM
INNTRKNGGRVIAVGTTSTRTLESVALEDGTIPAKSGWTEIFIYPGYKFKAIDCLITNFHLPESTLVMLVSALAGREHVL
HAYEVAVQEKYRFFSFGDAMFIK
>Mature_343_residues
MDLHDFYYDLPEELIAQDPLSDRSSSRLMVLDKETGEIEHKIFRDIVDYLKPGDCLVVNDTKVIPARLIGTKADTGATIE
VLLLKRLEDRQDTWEVLVKPGKKAKVGAKISFGEGKLIGEIIDIVEEGNRLIQFTYDGIFEEILDELGQMPLPPYITHKL
EDKNRYQTVYAKHEGSAAAPTAGLHFTTELLEKIKAMGVNIARVTLHVGLGTFRPVKVENILDHHMHSEFYVVTQEAADM
INNTRKNGGRVIAVGTTSTRTLESVALEDGTIPAKSGWTEIFIYPGYKFKAIDCLITNFHLPESTLVMLVSALAGREHVL
HAYEVAVQEKYRFFSFGDAMFIK

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=346, Percent_Identity=50, Blast_Score=329, Evalue=1e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38511; Mature: 38511

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLHDFYYDLPEELIAQDPLSDRSSSRLMVLDKETGEIEHKIFRDIVDYLKPGDCLVVND
CCCHHHHHCCCHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECC
TKVIPARLIGTKADTGATIEVLLLKRLEDRQDTWEVLVKPGKKAKVGAKISFGEGKLIGE
CCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEECCCCHHHHH
IIDIVEEGNRLIQFTYDGIFEEILDELGQMPLPPYITHKLEDKNRYQTVYAKHEGSAAAP
HHHHHHCCCEEEEEEHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCCC
TAGLHFTTELLEKIKAMGVNIARVTLHVGLGTFRPVKVENILDHHMHSEFYVVTQEAADM
CCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEHHHHHHHHCCCCEEEEEHHHHHH
INNTRKNGGRVIAVGTTSTRTLESVALEDGTIPAKSGWTEIFIYPGYKFKAIDCLITNFH
HHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEEEEEEECC
LPESTLVMLVSALAGREHVLHAYEVAVQEKYRFFSFGDAMFIK
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCEEEEC
>Mature Secondary Structure
MDLHDFYYDLPEELIAQDPLSDRSSSRLMVLDKETGEIEHKIFRDIVDYLKPGDCLVVND
CCCHHHHHCCCHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECC
TKVIPARLIGTKADTGATIEVLLLKRLEDRQDTWEVLVKPGKKAKVGAKISFGEGKLIGE
CCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEECCCCHHHHH
IIDIVEEGNRLIQFTYDGIFEEILDELGQMPLPPYITHKLEDKNRYQTVYAKHEGSAAAP
HHHHHHCCCEEEEEEHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCCC
TAGLHFTTELLEKIKAMGVNIARVTLHVGLGTFRPVKVENILDHHMHSEFYVVTQEAADM
CCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEHHHHHHHHCCCCEEEEEHHHHHH
INNTRKNGGRVIAVGTTSTRTLESVALEDGTIPAKSGWTEIFIYPGYKFKAIDCLITNFH
HHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEEEEEEECC
LPESTLVMLVSALAGREHVLHAYEVAVQEKYRFFSFGDAMFIK
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA