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The map label for this gene is topB [H]

Identifier: 238917173

GI number: 238917173

Start: 1265861

End: 1267927

Strand: Reverse

Name: topB [H]

Synonym: EUBELI_01246

Alternate gene names: 238917173

Gene position: 1267927-1265861 (Counterclockwise)

Preceding gene: 238917174

Following gene: 238917172

Centisome position: 59.13

GC content: 38.41

Gene sequence:

>2067_bases
ATGCTTCAGGAGAATAATAATTGTGTAATAGACACAGAAATGAGGGATATAATGGGAAAATCCGTATATATCGCGGAAAA
ACCAAGCGTGGCGCAGGAGTTTGCCAAGGCACTGCATGTGAACTTCACAAGAAAAGACGGATATCTTGAGGGTGGAGACC
ATATTGTGACATGGTGTGTCGGACATCTGGTAACTATGAGCTACCCGGATGCATATGATCCTAATCTGAAAAGATGGAGC
TTTGACACATTGCCATTCATACCAAAGGAATTCAAGTATGAGGTTATACCTGCAGTTGCGAAACAGTTTAATATAGTTAA
AGACATTCTTACAAGGAATGATGTTGAATATATATATGTGTGTACCGATTCAGGACGAGAGGGAGAATATATATACAGGC
TTGTGAGGCAGGAGGCAGGCGTTAAGGGAAAGAAAGAGCGCCGTGTGTGGATTGATTCGCAGACAGAGGAAGAGATTCTT
AAAGGAATTAATACAGCTAAAGATATATCTGAATATGATAATCTGAGTGATTCAGCGTATCTTCGTGCGAAGGAAGATTA
TCTTATGGGAATTAATTTCTCAAGAGTTCTTACATTGAAATATGGACGCAATATTGCCAATTATCTGCATCTTGACAGGG
CAGTTGTATCTGTCGGCAGGGTTATGACATGTGTTCTTGGAATGGTAGTAAGAAGGGAACGGGAGATAAGAAGCTTTGTT
AAAACTCCATTCTACAGGGTTATAGGTCAGGCACAGGTTGAGAATTCAACATTTGACGCAGAATGGCGTGTGAGTGATAA
GAGTATGTATGCGGGCACACCGTATTTGTATAAGGATAATGGTTTTAAGGACAGGGAGAAGGCTGAGGAGCTTGTTAAGT
TCTTATCTGACCCACTGCCGGCACAGGGAGTAGTTGATTCTATTGAGAGAAAGAAAGAAACCAAGAATCCACCACTTCTG
TACAACCTTGCCGAGATTCAGAATGAATGCAGCAAGTTGTTTAAGATAAGCCCTGATGAGACCCTTAATATTATTCAGGA
GCTGTATGAAAAGAAACTGGTTACTTATCCAAGAACTGATGCGAGAGTGTTATCAACAGCAGTAAGTAAGGAAATACATA
AGAATATAGGCGGACTTAGGAATTTTCCTCCTGTGAAGGAGATAGCAGAGCATATATTACAGAATAATATGCAGAAGGGA
ATTGAAAAGACAAGATACTGCAATGATAAAGCAATCACAGACCATTATGCGATTATTCCGACAGGACAGGGGTTTAATAA
TTTAAAGAACTTGTCAGTTACAGCTGCGAGAACCTATGAGATAATTGTAAGAAGATTTTTAAGTATATTCTATCCACCGG
CTATTTATCAGAAGGTAAGTATTATCAGTGATGTAAAGAATGAAAAGCTGTATGCTTCATTTAAAGTGCTTGTAGATGAA
GGTTATCTTAGCGTAGCTAAGAACTCATTTGCAAAGGCGAGAAATGATGCAAAAGTAAAGGATGATGATAGCGTCGATAA
TAAGGAAGATAATAAATGTGATGTAGCTTTATTTGAGGTATTGAAAAAATACAGAAAAGGTTCTACAATCACATTTAATG
GGTTTAATATCAAAGAAGGTGAAACATCACCTCCTAAGAGATATAATTCAGGTTCTATTATTCTTGCAATGGAGAATGCA
GGACAGCTTATTGAAGATGAGGAGCTGCGTGCCCAGATTAAGGGTAGTGGAATAGGAACAAGTGCTACAAGAGCAGAGAT
TCTTAAAAAACTTAATACTATTAAGTATATTTCTATTAATTCTAAGACACAGATTATTACGCCTACATTTTTAGGCGAGA
TAATATATGATGTTGTAGACAATTCTATCAGGCATCTGCTTAATCCGGAGCTTACGGCAAGCTGGGAAAAAGGACTTACA
GGTGTAGCAGAGGGGAATATTACAGCAGATGAGTATATGGAAAAGCTTGATGGTTTTGTTTCAAGAAGAACAAGGGCTGT
TATGGCATTAAGGAATCAGGTGGGGCTTGTAGGGCTTTTTAACGAGACTGCTAAGAATTATAAGTAA

Upstream 100 bases:

>100_bases
GATGCGGAAAAGACAATATATCAGGCTATTGTTGATATGAATATTCCGGAAGCAGATTATTTTATAAAAGAATATATACC
AGCTTATATGGGAACTCTTG

Downstream 100 bases:

>100_bases
ATGAGTGAAAGGAATTAAATATATGTGCGGAATTGCAGGATATGTAGGAGACAGAGAAAGCGCAGATGTACTTGTAGATG
CATTGTCTAAGCTTGAATAC

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 688; Mature: 688

Protein sequence:

>688_residues
MLQENNNCVIDTEMRDIMGKSVYIAEKPSVAQEFAKALHVNFTRKDGYLEGGDHIVTWCVGHLVTMSYPDAYDPNLKRWS
FDTLPFIPKEFKYEVIPAVAKQFNIVKDILTRNDVEYIYVCTDSGREGEYIYRLVRQEAGVKGKKERRVWIDSQTEEEIL
KGINTAKDISEYDNLSDSAYLRAKEDYLMGINFSRVLTLKYGRNIANYLHLDRAVVSVGRVMTCVLGMVVRREREIRSFV
KTPFYRVIGQAQVENSTFDAEWRVSDKSMYAGTPYLYKDNGFKDREKAEELVKFLSDPLPAQGVVDSIERKKETKNPPLL
YNLAEIQNECSKLFKISPDETLNIIQELYEKKLVTYPRTDARVLSTAVSKEIHKNIGGLRNFPPVKEIAEHILQNNMQKG
IEKTRYCNDKAITDHYAIIPTGQGFNNLKNLSVTAARTYEIIVRRFLSIFYPPAIYQKVSIISDVKNEKLYASFKVLVDE
GYLSVAKNSFAKARNDAKVKDDDSVDNKEDNKCDVALFEVLKKYRKGSTITFNGFNIKEGETSPPKRYNSGSIILAMENA
GQLIEDEELRAQIKGSGIGTSATRAEILKKLNTIKYISINSKTQIITPTFLGEIIYDVVDNSIRHLLNPELTASWEKGLT
GVAEGNITADEYMEKLDGFVSRRTRAVMALRNQVGLVGLFNETAKNYK

Sequences:

>Translated_688_residues
MLQENNNCVIDTEMRDIMGKSVYIAEKPSVAQEFAKALHVNFTRKDGYLEGGDHIVTWCVGHLVTMSYPDAYDPNLKRWS
FDTLPFIPKEFKYEVIPAVAKQFNIVKDILTRNDVEYIYVCTDSGREGEYIYRLVRQEAGVKGKKERRVWIDSQTEEEIL
KGINTAKDISEYDNLSDSAYLRAKEDYLMGINFSRVLTLKYGRNIANYLHLDRAVVSVGRVMTCVLGMVVRREREIRSFV
KTPFYRVIGQAQVENSTFDAEWRVSDKSMYAGTPYLYKDNGFKDREKAEELVKFLSDPLPAQGVVDSIERKKETKNPPLL
YNLAEIQNECSKLFKISPDETLNIIQELYEKKLVTYPRTDARVLSTAVSKEIHKNIGGLRNFPPVKEIAEHILQNNMQKG
IEKTRYCNDKAITDHYAIIPTGQGFNNLKNLSVTAARTYEIIVRRFLSIFYPPAIYQKVSIISDVKNEKLYASFKVLVDE
GYLSVAKNSFAKARNDAKVKDDDSVDNKEDNKCDVALFEVLKKYRKGSTITFNGFNIKEGETSPPKRYNSGSIILAMENA
GQLIEDEELRAQIKGSGIGTSATRAEILKKLNTIKYISINSKTQIITPTFLGEIIYDVVDNSIRHLLNPELTASWEKGLT
GVAEGNITADEYMEKLDGFVSRRTRAVMALRNQVGLVGLFNETAKNYK
>Mature_688_residues
MLQENNNCVIDTEMRDIMGKSVYIAEKPSVAQEFAKALHVNFTRKDGYLEGGDHIVTWCVGHLVTMSYPDAYDPNLKRWS
FDTLPFIPKEFKYEVIPAVAKQFNIVKDILTRNDVEYIYVCTDSGREGEYIYRLVRQEAGVKGKKERRVWIDSQTEEEIL
KGINTAKDISEYDNLSDSAYLRAKEDYLMGINFSRVLTLKYGRNIANYLHLDRAVVSVGRVMTCVLGMVVRREREIRSFV
KTPFYRVIGQAQVENSTFDAEWRVSDKSMYAGTPYLYKDNGFKDREKAEELVKFLSDPLPAQGVVDSIERKKETKNPPLL
YNLAEIQNECSKLFKISPDETLNIIQELYEKKLVTYPRTDARVLSTAVSKEIHKNIGGLRNFPPVKEIAEHILQNNMQKG
IEKTRYCNDKAITDHYAIIPTGQGFNNLKNLSVTAARTYEIIVRRFLSIFYPPAIYQKVSIISDVKNEKLYASFKVLVDE
GYLSVAKNSFAKARNDAKVKDDDSVDNKEDNKCDVALFEVLKKYRKGSTITFNGFNIKEGETSPPKRYNSGSIILAMENA
GQLIEDEELRAQIKGSGIGTSATRAEILKKLNTIKYISINSKTQIITPTFLGEIIYDVVDNSIRHLLNPELTASWEKGLT
GVAEGNITADEYMEKLDGFVSRRTRAVMALRNQVGLVGLFNETAKNYK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=596, Percent_Identity=22.6510067114094, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1788061, Length=644, Percent_Identity=30.4347826086957, Blast_Score=276, Evalue=4e-75,
Organism=Escherichia coli, GI1787529, Length=671, Percent_Identity=23.5469448584203, Blast_Score=115, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17555378, Length=671, Percent_Identity=23.9940387481371, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323263, Length=536, Percent_Identity=24.4402985074627, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585251, Length=610, Percent_Identity=23.1147540983607, Blast_Score=105, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 78315; Mature: 78315

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQENNNCVIDTEMRDIMGKSVYIAEKPSVAQEFAKALHVNFTRKDGYLEGGDHIVTWCV
CCCCCCCEEEECHHHHHCCCEEEEECCCHHHHHHHHHHHCEEECCCCCCCCCCHHHHHHH
GHLVTMSYPDAYDPNLKRWSFDTLPFIPKEFKYEVIPAVAKQFNIVKDILTRNDVEYIYV
HHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
CTDSGREGEYIYRLVRQEAGVKGKKERRVWIDSQTEEEILKGINTAKDISEYDNLSDSAY
ECCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHCCHHHHHHHHCCCCCCCE
LRAKEDYLMGINFSRVLTLKYGRNIANYLHLDRAVVSVGRVMTCVLGMVVRREREIRSFV
EEECCCEEECCCHHHEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTPFYRVIGQAQVENSTFDAEWRVSDKSMYAGTPYLYKDNGFKDREKAEELVKFLSDPLP
HCHHHHHHHHHHHCCCCCCCEEEECCCCEECCCCEEECCCCCCCHHHHHHHHHHHCCCCC
AQGVVDSIERKKETKNPPLLYNLAEIQNECSKLFKISPDETLNIIQELYEKKLVTYPRTD
CHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCH
ARVLSTAVSKEIHKNIGGLRNFPPVKEIAEHILQNNMQKGIEKTRYCNDKAITDHYAIIP
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
TGQGFNNLKNLSVTAARTYEIIVRRFLSIFYPPAIYQKVSIISDVKNEKLYASFKVLVDE
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHEEC
GYLSVAKNSFAKARNDAKVKDDDSVDNKEDNKCDVALFEVLKKYRKGSTITFNGFNIKEG
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCC
ETSPPKRYNSGSIILAMENAGQLIEDEELRAQIKGSGIGTSATRAEILKKLNTIKYISIN
CCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCEEEEEEC
SKTQIITPTFLGEIIYDVVDNSIRHLLNPELTASWEKGLTGVAEGNITADEYMEKLDGFV
CCCEEECHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHCCCCCCCCCCCHHHHHHHHHHHH
SRRTRAVMALRNQVGLVGLFNETAKNYK
HHHHHHHHHHHHCCCEEEEEHHHHCCCC
>Mature Secondary Structure
MLQENNNCVIDTEMRDIMGKSVYIAEKPSVAQEFAKALHVNFTRKDGYLEGGDHIVTWCV
CCCCCCCEEEECHHHHHCCCEEEEECCCHHHHHHHHHHHCEEECCCCCCCCCCHHHHHHH
GHLVTMSYPDAYDPNLKRWSFDTLPFIPKEFKYEVIPAVAKQFNIVKDILTRNDVEYIYV
HHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
CTDSGREGEYIYRLVRQEAGVKGKKERRVWIDSQTEEEILKGINTAKDISEYDNLSDSAY
ECCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHCCHHHHHHHHCCCCCCCE
LRAKEDYLMGINFSRVLTLKYGRNIANYLHLDRAVVSVGRVMTCVLGMVVRREREIRSFV
EEECCCEEECCCHHHEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTPFYRVIGQAQVENSTFDAEWRVSDKSMYAGTPYLYKDNGFKDREKAEELVKFLSDPLP
HCHHHHHHHHHHHCCCCCCCEEEECCCCEECCCCEEECCCCCCCHHHHHHHHHHHCCCCC
AQGVVDSIERKKETKNPPLLYNLAEIQNECSKLFKISPDETLNIIQELYEKKLVTYPRTD
CHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCH
ARVLSTAVSKEIHKNIGGLRNFPPVKEIAEHILQNNMQKGIEKTRYCNDKAITDHYAIIP
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
TGQGFNNLKNLSVTAARTYEIIVRRFLSIFYPPAIYQKVSIISDVKNEKLYASFKVLVDE
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHEEC
GYLSVAKNSFAKARNDAKVKDDDSVDNKEDNKCDVALFEVLKKYRKGSTITFNGFNIKEG
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCC
ETSPPKRYNSGSIILAMENAGQLIEDEELRAQIKGSGIGTSATRAEILKKLNTIKYISIN
CCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCEEEEEEC
SKTQIITPTFLGEIIYDVVDNSIRHLLNPELTASWEKGLTGVAEGNITADEYMEKLDGFV
CCCEEECHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHCCCCCCCCCCCHHHHHHHHHHHH
SRRTRAVMALRNQVGLVGLFNETAKNYK
HHHHHHHHHHHHCCCEEEEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]